Amino acid dipepetide frequency for Roseburia sp. 499

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.427AlaAla: 5.427 ± 0.102	0.905AlaCys: 0.905 ± 0.028	3.903AlaAsp: 3.903 ± 0.071	5.698AlaGlu: 5.698 ± 0.079	2.745AlaPhe: 2.745 ± 0.055	5.216AlaGly: 5.216 ± 0.08	0.993AlaHis: 0.993 ± 0.031	5.09AlaIle: 5.09 ± 0.089	4.906AlaLys: 4.906 ± 0.086	6.052AlaLeu: 6.052 ± 0.087	2.299AlaMet: 2.299 ± 0.051	2.622AlaAsn: 2.622 ± 0.062	1.696AlaPro: 1.696 ± 0.04	2.301AlaGln: 2.301 ± 0.058	2.465AlaArg: 2.465 ± 0.05	3.613AlaSer: 3.613 ± 0.068	3.213AlaThr: 3.213 ± 0.078	5.513AlaVal: 5.513 ± 0.073	0.531AlaTrp: 0.531 ± 0.023	2.762AlaTyr: 2.762 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.879CysAla: 0.879 ± 0.029	0.236CysCys: 0.236 ± 0.018	0.765CysAsp: 0.765 ± 0.031	1.0CysGlu: 1.0 ± 0.03	0.637CysPhe: 0.637 ± 0.031	1.331CysGly: 1.331 ± 0.039	0.281CysHis: 0.281 ± 0.018	1.185CysIle: 1.185 ± 0.037	0.944CysLys: 0.944 ± 0.025	1.062CysLeu: 1.062 ± 0.041	0.48CysMet: 0.48 ± 0.02	0.62CysAsn: 0.62 ± 0.028	0.565CysPro: 0.565 ± 0.028	0.484CysGln: 0.484 ± 0.021	0.597CysArg: 0.597 ± 0.025	0.876CysSer: 0.876 ± 0.037	0.672CysThr: 0.672 ± 0.032	0.993CysVal: 0.993 ± 0.032	0.112CysTrp: 0.112 ± 0.011	0.637CysTyr: 0.637 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.814AspAla: 3.814 ± 0.071	0.732AspCys: 0.732 ± 0.028	2.64AspAsp: 2.64 ± 0.064	4.69AspGlu: 4.69 ± 0.079	2.611AspPhe: 2.611 ± 0.054	4.122AspGly: 4.122 ± 0.08	0.588AspHis: 0.588 ± 0.028	4.835AspIle: 4.835 ± 0.077	3.799AspLys: 3.799 ± 0.066	3.944AspLeu: 3.944 ± 0.068	2.014AspMet: 2.014 ± 0.05	2.482AspAsn: 2.482 ± 0.051	1.206AspPro: 1.206 ± 0.036	0.991AspGln: 0.991 ± 0.026	1.84AspArg: 1.84 ± 0.044	2.998AspSer: 2.998 ± 0.062	3.103AspThr: 3.103 ± 0.064	3.793AspVal: 3.793 ± 0.064	0.514AspTrp: 0.514 ± 0.024	2.992AspTyr: 2.992 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	5.518GluAla: 5.518 ± 0.086	0.97GluCys: 0.97 ± 0.032	4.497GluAsp: 4.497 ± 0.072	9.541GluGlu: 9.541 ± 0.148	2.965GluPhe: 2.965 ± 0.057	4.691GluGly: 4.691 ± 0.063	1.44GluHis: 1.44 ± 0.044	6.487GluIle: 6.487 ± 0.087	8.472GluLys: 8.472 ± 0.121	7.506GluLeu: 7.506 ± 0.102	2.787GluMet: 2.787 ± 0.063	5.113GluAsn: 5.113 ± 0.074	1.921GluPro: 1.921 ± 0.054	3.851GluGln: 3.851 ± 0.081	3.703GluArg: 3.703 ± 0.071	3.864GluSer: 3.864 ± 0.066	3.987GluThr: 3.987 ± 0.071	5.422GluVal: 5.422 ± 0.081	0.732GluTrp: 0.732 ± 0.028	3.683GluTyr: 3.683 ± 0.079	0.0GluXaa: 0.0 ± 0.0
Phe	2.753PheAla: 2.753 ± 0.062	0.782PheCys: 0.782 ± 0.035	2.367PheAsp: 2.367 ± 0.051	2.96PheGlu: 2.96 ± 0.055	1.824PhePhe: 1.824 ± 0.052	2.856PheGly: 2.856 ± 0.06	0.829PheHis: 0.829 ± 0.03	2.744PheIle: 2.744 ± 0.065	2.031PheLys: 2.031 ± 0.042	3.92PheLeu: 3.92 ± 0.074	1.168PheMet: 1.168 ± 0.038	1.494PheAsn: 1.494 ± 0.036	1.278PhePro: 1.278 ± 0.043	1.579PheGln: 1.579 ± 0.044	1.534PheArg: 1.534 ± 0.039	2.945PheSer: 2.945 ± 0.065	2.186PheThr: 2.186 ± 0.048	2.922PheVal: 2.922 ± 0.057	0.449PheTrp: 0.449 ± 0.022	1.879PheTyr: 1.879 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.458GlyAla: 4.458 ± 0.084	1.122GlyCys: 1.122 ± 0.034	3.109GlyAsp: 3.109 ± 0.063	4.947GlyGlu: 4.947 ± 0.074	2.958GlyPhe: 2.958 ± 0.065	4.104GlyGly: 4.104 ± 0.074	1.109GlyHis: 1.109 ± 0.039	6.332GlyIle: 6.332 ± 0.083	5.832GlyLys: 5.832 ± 0.08	5.108GlyLeu: 5.108 ± 0.093	2.576GlyMet: 2.576 ± 0.051	3.4GlyAsn: 3.4 ± 0.071	0.99GlyPro: 0.99 ± 0.036	1.933GlyGln: 1.933 ± 0.043	2.565GlyArg: 2.565 ± 0.054	3.584GlySer: 3.584 ± 0.065	4.126GlyThr: 4.126 ± 0.091	4.756GlyVal: 4.756 ± 0.075	0.65GlyTrp: 0.65 ± 0.028	3.259GlyTyr: 3.259 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	0.903HisAla: 0.903 ± 0.033	0.283HisCys: 0.283 ± 0.017	0.862HisAsp: 0.862 ± 0.033	1.016HisGlu: 1.016 ± 0.035	0.801HisPhe: 0.801 ± 0.031	1.112HisGly: 1.112 ± 0.04	0.391HisHis: 0.391 ± 0.025	1.354HisIle: 1.354 ± 0.036	1.006HisLys: 1.006 ± 0.032	1.369HisLeu: 1.369 ± 0.039	0.596HisMet: 0.596 ± 0.024	0.76HisAsn: 0.76 ± 0.027	0.769HisPro: 0.769 ± 0.032	0.552HisGln: 0.552 ± 0.022	0.651HisArg: 0.651 ± 0.028	0.917HisSer: 0.917 ± 0.034	0.893HisThr: 0.893 ± 0.031	1.01HisVal: 1.01 ± 0.031	0.176HisTrp: 0.176 ± 0.014	0.752HisTyr: 0.752 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.727IleAla: 5.727 ± 0.079	1.33IleCys: 1.33 ± 0.04	4.254IleAsp: 4.254 ± 0.065	5.937IleGlu: 5.937 ± 0.098	3.072IlePhe: 3.072 ± 0.06	5.341IleGly: 5.341 ± 0.084	1.386IleHis: 1.386 ± 0.034	5.506IleIle: 5.506 ± 0.086	4.547IleLys: 4.547 ± 0.076	7.344IleLeu: 7.344 ± 0.1	2.268IleMet: 2.268 ± 0.056	3.23IleAsn: 3.23 ± 0.064	3.028IlePro: 3.028 ± 0.054	2.612IleGln: 2.612 ± 0.043	3.252IleArg: 3.252 ± 0.072	5.325IleSer: 5.325 ± 0.082	4.453IleThr: 4.453 ± 0.071	5.177IleVal: 5.177 ± 0.076	0.64IleTrp: 0.64 ± 0.026	3.025IleTyr: 3.025 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.745LysAla: 4.745 ± 0.073	0.84LysCys: 0.84 ± 0.035	3.948LysAsp: 3.948 ± 0.061	8.231LysGlu: 8.231 ± 0.114	2.072LysPhe: 2.072 ± 0.047	4.296LysGly: 4.296 ± 0.064	1.086LysHis: 1.086 ± 0.031	5.642LysIle: 5.642 ± 0.081	6.702LysLys: 6.702 ± 0.076	5.665LysLeu: 5.665 ± 0.079	2.484LysMet: 2.484 ± 0.055	4.052LysAsn: 4.052 ± 0.058	1.843LysPro: 1.843 ± 0.043	2.779LysGln: 2.779 ± 0.055	3.303LysArg: 3.303 ± 0.064	3.747LysSer: 3.747 ± 0.062	3.606LysThr: 3.606 ± 0.061	4.88LysVal: 4.88 ± 0.077	0.594LysTrp: 0.594 ± 0.023	3.296LysTyr: 3.296 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	6.038LeuAla: 6.038 ± 0.077	1.361LeuCys: 1.361 ± 0.041	4.753LeuAsp: 4.753 ± 0.078	7.459LeuGlu: 7.459 ± 0.089	3.584LeuPhe: 3.584 ± 0.076	5.468LeuGly: 5.468 ± 0.085	1.36LeuHis: 1.36 ± 0.038	5.799LeuIle: 5.799 ± 0.09	6.416LeuLys: 6.416 ± 0.085	8.208LeuLeu: 8.208 ± 0.126	2.59LeuMet: 2.59 ± 0.056	3.888LeuAsn: 3.888 ± 0.064	3.085LeuPro: 3.085 ± 0.058	3.106LeuGln: 3.106 ± 0.064	3.352LeuArg: 3.352 ± 0.061	5.8LeuSer: 5.8 ± 0.082	4.656LeuThr: 4.656 ± 0.079	5.541LeuVal: 5.541 ± 0.075	0.708LeuTrp: 0.708 ± 0.027	3.299LeuTyr: 3.299 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	2.364MetAla: 2.364 ± 0.058	0.369MetCys: 0.369 ± 0.019	1.874MetAsp: 1.874 ± 0.045	3.282MetGlu: 3.282 ± 0.066	1.07MetPhe: 1.07 ± 0.036	2.11MetGly: 2.11 ± 0.039	0.432MetHis: 0.432 ± 0.02	2.398MetIle: 2.398 ± 0.054	2.85MetLys: 2.85 ± 0.05	2.779MetLeu: 2.779 ± 0.062	0.965MetMet: 0.965 ± 0.032	1.681MetAsn: 1.681 ± 0.043	1.136MetPro: 1.136 ± 0.035	1.291MetGln: 1.291 ± 0.039	1.258MetArg: 1.258 ± 0.038	1.776MetSer: 1.776 ± 0.053	1.632MetThr: 1.632 ± 0.039	2.111MetVal: 2.111 ± 0.051	0.214MetTrp: 0.214 ± 0.014	1.056MetTyr: 1.056 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	3.14AsnAla: 3.14 ± 0.058	0.702AsnCys: 0.702 ± 0.029	2.207AsnAsp: 2.207 ± 0.057	3.307AsnGlu: 3.307 ± 0.064	1.753AsnPhe: 1.753 ± 0.045	3.716AsnGly: 3.716 ± 0.083	0.926AsnHis: 0.926 ± 0.03	4.057AsnIle: 4.057 ± 0.077	2.865AsnLys: 2.865 ± 0.057	4.052AsnLeu: 4.052 ± 0.069	1.657AsnMet: 1.657 ± 0.042	2.088AsnAsn: 2.088 ± 0.05	2.005AsnPro: 2.005 ± 0.047	1.893AsnGln: 1.893 ± 0.048	1.999AsnArg: 1.999 ± 0.043	2.574AsnSer: 2.574 ± 0.061	2.54AsnThr: 2.54 ± 0.059	3.169AsnVal: 3.169 ± 0.059	0.469AsnTrp: 0.469 ± 0.02	2.128AsnTyr: 2.128 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	1.798ProAla: 1.798 ± 0.042	0.399ProCys: 0.399 ± 0.022	1.904ProAsp: 1.904 ± 0.047	3.285ProGlu: 3.285 ± 0.066	1.339ProPhe: 1.339 ± 0.037	1.868ProGly: 1.868 ± 0.043	0.443ProHis: 0.443 ± 0.02	2.107ProIle: 2.107 ± 0.039	1.912ProLys: 1.912 ± 0.044	2.328ProLeu: 2.328 ± 0.05	0.932ProMet: 0.932 ± 0.03	1.248ProAsn: 1.248 ± 0.038	0.571ProPro: 0.571 ± 0.026	1.022ProGln: 1.022 ± 0.038	0.847ProArg: 0.847 ± 0.032	1.493ProSer: 1.493 ± 0.036	1.445ProThr: 1.445 ± 0.044	2.616ProVal: 2.616 ± 0.05	0.291ProTrp: 0.291 ± 0.021	1.358ProTyr: 1.358 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.412GlnAla: 2.412 ± 0.057	0.392GlnCys: 0.392 ± 0.018	1.553GlnAsp: 1.553 ± 0.043	3.593GlnGlu: 3.593 ± 0.068	1.238GlnPhe: 1.238 ± 0.036	2.077GlnGly: 2.077 ± 0.047	0.466GlnHis: 0.466 ± 0.023	2.834GlnIle: 2.834 ± 0.057	3.255GlnLys: 3.255 ± 0.048	3.054GlnLeu: 3.054 ± 0.057	1.33GlnMet: 1.33 ± 0.038	1.904GlnAsn: 1.904 ± 0.051	0.832GlnPro: 0.832 ± 0.032	1.645GlnGln: 1.645 ± 0.05	1.433GlnArg: 1.433 ± 0.038	1.656GlnSer: 1.656 ± 0.041	1.662GlnThr: 1.662 ± 0.045	2.339GlnVal: 2.339 ± 0.053	0.322GlnTrp: 0.322 ± 0.019	1.419GlnTyr: 1.419 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.237ArgAla: 2.237 ± 0.047	0.53ArgCys: 0.53 ± 0.025	1.926ArgAsp: 1.926 ± 0.047	3.855ArgGlu: 3.855 ± 0.07	1.672ArgPhe: 1.672 ± 0.041	2.15ArgGly: 2.15 ± 0.052	0.573ArgHis: 0.573 ± 0.024	3.394ArgIle: 3.394 ± 0.07	3.583ArgLys: 3.583 ± 0.059	3.286ArgLeu: 3.286 ± 0.065	1.533ArgMet: 1.533 ± 0.038	2.104ArgAsn: 2.104 ± 0.041	1.016ArgPro: 1.016 ± 0.039	1.562ArgGln: 1.562 ± 0.036	1.891ArgArg: 1.891 ± 0.051	1.863ArgSer: 1.863 ± 0.044	2.006ArgThr: 2.006 ± 0.042	2.516ArgVal: 2.516 ± 0.065	0.31ArgTrp: 0.31 ± 0.02	1.68ArgTyr: 1.68 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.75SerAla: 3.75 ± 0.073	0.803SerCys: 0.803 ± 0.028	3.172SerAsp: 3.172 ± 0.071	4.366SerGlu: 4.366 ± 0.076	2.618SerPhe: 2.618 ± 0.049	4.489SerGly: 4.489 ± 0.077	0.984SerHis: 0.984 ± 0.031	4.428SerIle: 4.428 ± 0.068	3.549SerLys: 3.549 ± 0.062	4.837SerLeu: 4.837 ± 0.078	1.872SerMet: 1.872 ± 0.043	2.596SerAsn: 2.596 ± 0.057	1.533SerPro: 1.533 ± 0.038	1.958SerGln: 1.958 ± 0.045	2.299SerArg: 2.299 ± 0.051	3.32SerSer: 3.32 ± 0.066	2.802SerThr: 2.802 ± 0.057	4.262SerVal: 4.262 ± 0.07	0.494SerTrp: 0.494 ± 0.022	2.61SerTyr: 2.61 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.856ThrAla: 3.856 ± 0.069	0.624ThrCys: 0.624 ± 0.026	2.961ThrAsp: 2.961 ± 0.066	4.334ThrGlu: 4.334 ± 0.073	2.097ThrPhe: 2.097 ± 0.054	4.294ThrGly: 4.294 ± 0.078	0.784ThrHis: 0.784 ± 0.029	4.096ThrIle: 4.096 ± 0.065	3.121ThrLys: 3.121 ± 0.057	4.737ThrLeu: 4.737 ± 0.081	1.469ThrMet: 1.469 ± 0.036	2.215ThrAsn: 2.215 ± 0.054	1.95ThrPro: 1.95 ± 0.047	1.571ThrGln: 1.571 ± 0.04	1.857ThrArg: 1.857 ± 0.05	2.978ThrSer: 2.978 ± 0.067	2.942ThrThr: 2.942 ± 0.066	4.223ThrVal: 4.223 ± 0.078	0.42ThrTrp: 0.42 ± 0.021	2.227ThrTyr: 2.227 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	4.879ValAla: 4.879 ± 0.078	1.189ValCys: 1.189 ± 0.035	3.779ValAsp: 3.779 ± 0.06	5.435ValGlu: 5.435 ± 0.081	2.996ValPhe: 2.996 ± 0.066	4.263ValGly: 4.263 ± 0.078	1.038ValHis: 1.038 ± 0.033	5.376ValIle: 5.376 ± 0.074	4.794ValLys: 4.794 ± 0.078	6.556ValLeu: 6.556 ± 0.094	2.066ValMet: 2.066 ± 0.048	3.155ValAsn: 3.155 ± 0.06	2.252ValPro: 2.252 ± 0.042	2.06ValGln: 2.06 ± 0.051	2.592ValArg: 2.592 ± 0.053	4.474ValSer: 4.474 ± 0.081	4.149ValThr: 4.149 ± 0.08	5.203ValVal: 5.203 ± 0.088	0.637ValTrp: 0.637 ± 0.026	2.781ValTyr: 2.781 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.494TrpAla: 0.494 ± 0.022	0.136TrpCys: 0.136 ± 0.013	0.509TrpAsp: 0.509 ± 0.022	0.662TrpGlu: 0.662 ± 0.027	0.433TrpPhe: 0.433 ± 0.018	0.549TrpGly: 0.549 ± 0.023	0.171TrpHis: 0.171 ± 0.014	0.646TrpIle: 0.646 ± 0.025	0.779TrpLys: 0.779 ± 0.029	0.82TrpLeu: 0.82 ± 0.032	0.316TrpMet: 0.316 ± 0.019	0.588TrpAsn: 0.588 ± 0.028	0.152TrpPro: 0.152 ± 0.014	0.338TrpGln: 0.338 ± 0.021	0.34TrpArg: 0.34 ± 0.019	0.465TrpSer: 0.465 ± 0.022	0.341TrpThr: 0.341 ± 0.02	0.491TrpVal: 0.491 ± 0.025	0.091TrpTrp: 0.091 ± 0.01	0.388TrpTyr: 0.388 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.755TyrAla: 2.755 ± 0.056	0.649TyrCys: 0.649 ± 0.029	2.727TyrAsp: 2.727 ± 0.059	3.552TyrGlu: 3.552 ± 0.059	2.017TyrPhe: 2.017 ± 0.047	2.868TyrGly: 2.868 ± 0.057	0.895TyrHis: 0.895 ± 0.031	3.144TyrIle: 3.144 ± 0.062	2.448TyrLys: 2.448 ± 0.053	3.845TyrLeu: 3.845 ± 0.074	1.246TyrMet: 1.246 ± 0.038	2.006TyrAsn: 2.006 ± 0.045	1.377TyrPro: 1.377 ± 0.04	1.885TyrGln: 1.885 ± 0.046	1.912TyrArg: 1.912 ± 0.047	2.476TyrSer: 2.476 ± 0.056	2.339TyrThr: 2.339 ± 0.054	2.737TyrVal: 2.737 ± 0.052	0.352TyrTrp: 0.352 ± 0.018	2.198TyrTyr: 2.198 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3105 proteins (974933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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