Fusarium oxysporum f. sp. radicis-cucumerinum
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16940 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H3FS04|A0A2H3FS04_FUSOX FAD-binding FR-type domain-containing protein OS=Fusarium oxysporum f. sp. radicis-cucumerinum OX=327505 GN=AU210_016438 PE=4 SV=1
MM1 pKa = 7.8 ASTFEE6 pKa = 4.42 SPVGIDD12 pKa = 3.42 PKK14 pKa = 10.18 KK15 pKa = 8.89 TSLNSSSPTPEE26 pKa = 3.61 MLQYY30 pKa = 10.49 DD31 pKa = 4.49 APLQDD36 pKa = 3.68 MQLDD40 pKa = 3.95 DD41 pKa = 4.41 AQTVLLSLSGYY52 pKa = 10.22 EE53 pKa = 4.07 DD54 pKa = 5.35 ANGFWYY60 pKa = 10.26 CHH62 pKa = 5.81 NCPISHH68 pKa = 7.27 LDD70 pKa = 3.38 EE71 pKa = 4.71 AQYY74 pKa = 8.9 TAPVDD79 pKa = 3.73 GDD81 pKa = 3.77 EE82 pKa = 5.64 LNTFDD87 pKa = 5.78 PLTDD91 pKa = 3.42 MLGSKK96 pKa = 10.48 LCDD99 pKa = 3.25 LDD101 pKa = 4.22 SFEE104 pKa = 5.23 EE105 pKa = 4.34 KK106 pKa = 10.54 ASPSDD111 pKa = 4.54 GISTLCQFQGNDD123 pKa = 3.3 SVLEE127 pKa = 4.05 ADD129 pKa = 3.66 TPEE132 pKa = 3.98 IVIVQQGDD140 pKa = 2.92 ACYY143 pKa = 10.37 DD144 pKa = 3.13 IVRR147 pKa = 11.84 IQEE150 pKa = 4.11 NKK152 pKa = 9.46 SGRR155 pKa = 11.84 NLGEE159 pKa = 3.82 IADD162 pKa = 3.97 IGIDD166 pKa = 3.65 PAALHH171 pKa = 5.9 YY172 pKa = 10.3 SWKK175 pKa = 9.94 EE176 pKa = 3.86 CNN178 pKa = 3.39
Molecular weight: 19.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.897
Patrickios 0.604
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A2H3H0G6|A0A2H3H0G6_FUSOX Zn(2)-C6 fungal-type domain-containing protein OS=Fusarium oxysporum f. sp. radicis-cucumerinum OX=327505 GN=AU210_009703 PE=4 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.63 RR8 pKa = 11.84 HH9 pKa = 4.04 TAPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APAHH26 pKa = 4.84 SHH28 pKa = 5.33 RR29 pKa = 11.84 HH30 pKa = 4.03 TTTTTTTTTKK40 pKa = 9.5 PRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 GMFGGGAGRR53 pKa = 11.84 RR54 pKa = 11.84 THH56 pKa = 6.46 ATTTAPVHH64 pKa = 4.87 HH65 pKa = 6.63 HH66 pKa = 5.14 QRR68 pKa = 11.84 RR69 pKa = 11.84 PSMKK73 pKa = 10.02 DD74 pKa = 2.95 KK75 pKa = 11.41 VSGALLKK82 pKa = 11.04 LKK84 pKa = 10.68 GSLTRR89 pKa = 11.84 RR90 pKa = 11.84 PGVKK94 pKa = 9.89 AAGTRR99 pKa = 11.84 RR100 pKa = 11.84 MRR102 pKa = 11.84 GTDD105 pKa = 3.02 GRR107 pKa = 11.84 GARR110 pKa = 11.84 HH111 pKa = 5.87 HH112 pKa = 7.23 RR113 pKa = 11.84 YY114 pKa = 9.44
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.023
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.296
Grimsley 12.72
Solomon 13.173
Lehninger 13.086
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.018
IPC_peptide 13.188
IPC2_peptide 12.164
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16567
373
16940
8032873
66
7604
474.2
52.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.294 ± 0.015
1.345 ± 0.009
5.811 ± 0.013
6.159 ± 0.018
3.833 ± 0.013
6.744 ± 0.018
2.4 ± 0.009
5.207 ± 0.015
4.992 ± 0.019
8.934 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.007
3.804 ± 0.009
5.803 ± 0.023
3.997 ± 0.012
5.837 ± 0.019
8.033 ± 0.019
6.024 ± 0.027
6.117 ± 0.013
1.586 ± 0.007
2.853 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here