Candidatus Tokpelaia sp. JSC161

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Tokpelaia; unclassified Candidatus Tokpelaia

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368A1K7|A0A368A1K7_9RHIZ Phosphoglycerate kinase OS=Candidatus Tokpelaia sp. JSC161 OX=2079007 GN=pgk PE=3 SV=1
MM1 pKa = 7.53TYY3 pKa = 10.67VITDD7 pKa = 3.14NCIGCKK13 pKa = 9.08YY14 pKa = 7.85TDD16 pKa = 3.95CVEE19 pKa = 4.15VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.21NMLVIHH36 pKa = 7.24PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.44PEE49 pKa = 5.05CPAEE53 pKa = 4.43AIKK56 pKa = 10.68PDD58 pKa = 3.9TDD60 pKa = 3.8PGLEE64 pKa = 3.7KK65 pKa = 10.35WLTLNAEE72 pKa = 4.68YY73 pKa = 10.67SLKK76 pKa = 10.0WPNISIKK83 pKa = 9.92TSPLPQAEE91 pKa = 4.27TMDD94 pKa = 4.82GINSKK99 pKa = 10.27LEE101 pKa = 4.05KK102 pKa = 10.64YY103 pKa = 10.09FSKK106 pKa = 11.04NPGKK110 pKa = 10.89GDD112 pKa = 3.28VRR114 pKa = 11.84EE115 pKa = 4.13PP116 pKa = 3.34

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368A5B1|A0A368A5B1_9RHIZ Riboflavin synthase OS=Candidatus Tokpelaia sp. JSC161 OX=2079007 GN=ribE PE=4 SV=1
MM1 pKa = 7.36NLMVYY6 pKa = 10.45ALDD9 pKa = 3.56GRR11 pKa = 11.84VIGEE15 pKa = 3.88IAISEE20 pKa = 4.66SIFCLEE26 pKa = 4.04PRR28 pKa = 11.84RR29 pKa = 11.84DD30 pKa = 3.32LLQRR34 pKa = 11.84VVSWQLACRR43 pKa = 11.84QRR45 pKa = 11.84GTHH48 pKa = 5.17QSQSRR53 pKa = 11.84ADD55 pKa = 3.71VSCTGSKK62 pKa = 9.41MYY64 pKa = 9.72KK65 pKa = 9.56QKK67 pKa = 9.16GTGRR71 pKa = 11.84ARR73 pKa = 11.84HH74 pKa = 5.93SSSSVPQFRR83 pKa = 11.84GGGKK87 pKa = 9.23AHH89 pKa = 6.41GPVSRR94 pKa = 11.84SHH96 pKa = 7.17AYY98 pKa = 8.3TIPKK102 pKa = 9.33KK103 pKa = 10.71VRR105 pKa = 11.84ALALRR110 pKa = 11.84HH111 pKa = 5.45ALSVKK116 pKa = 9.14MQMKK120 pKa = 10.6KK121 pKa = 10.88LMILDD126 pKa = 4.69AFTLSEE132 pKa = 4.33PKK134 pKa = 9.63TRR136 pKa = 11.84LLVNFFIGLRR146 pKa = 11.84LKK148 pKa = 10.11NALMIGARR156 pKa = 11.84EE157 pKa = 3.39IDD159 pKa = 3.72LNFRR163 pKa = 11.84RR164 pKa = 11.84AVSNIPTIDD173 pKa = 3.55TLPVQGVNVYY183 pKa = 10.73DD184 pKa = 3.44ILRR187 pKa = 11.84RR188 pKa = 11.84DD189 pKa = 3.45NLVISRR195 pKa = 11.84GALEE199 pKa = 4.08LLEE202 pKa = 4.66EE203 pKa = 4.19RR204 pKa = 11.84LKK206 pKa = 11.29

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

891

0

891

290539

29

2464

326.1

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.512 ± 0.081

1.186 ± 0.03

4.954 ± 0.059

5.895 ± 0.083

4.697 ± 0.074

6.768 ± 0.089

2.425 ± 0.036

8.659 ± 0.081

6.002 ± 0.063

10.352 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.04

4.2 ± 0.068

3.745 ± 0.045

3.351 ± 0.06

5.909 ± 0.066

7.045 ± 0.062

4.794 ± 0.062

6.048 ± 0.093

1.0 ± 0.025

2.889 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski