Flavivirga rizhaonensis
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4045 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S1E2P9|A0A4S1E2P9_9FLAO AhpC/TSA family protein OS=Flavivirga rizhaonensis OX=2559571 GN=EM932_01795 PE=4 SV=1
MM1 pKa = 7.24 NKK3 pKa = 9.42 IMMHH7 pKa = 6.86 LLQKK11 pKa = 9.94 KK12 pKa = 9.1 LQMLTIVFLSSMGAMAQAEE31 pKa = 4.92 YY32 pKa = 9.02 ITTWNTTNAGVSNSNSIIIPAIGGRR57 pKa = 11.84 YY58 pKa = 9.69 DD59 pKa = 3.41 VDD61 pKa = 3.14 IGNDD65 pKa = 3.41 GSYY68 pKa = 11.05 EE69 pKa = 3.98 LLNQTGRR76 pKa = 11.84 ITIDD80 pKa = 3.3 VTAHH84 pKa = 6.39 GYY86 pKa = 8.9 AAGVIQIALRR96 pKa = 11.84 NAISGAGNLEE106 pKa = 3.98 QIQFEE111 pKa = 4.18 PSGTNTDD118 pKa = 2.89 NGKK121 pKa = 9.47 IVSIDD126 pKa = 2.98 QWGNSIVWLSMHH138 pKa = 6.34 SAYY141 pKa = 10.33 HH142 pKa = 5.28 SCINLDD148 pKa = 3.38 VVAFDD153 pKa = 5.16 APNLSQVFNMILMFANCTSMVGTPAFNTWDD183 pKa = 3.56 VSNVTTTGGMFGDD196 pKa = 4.15 TPFNQPLNNWDD207 pKa = 3.45 VSNVTNMGFMFSNCPFNQPIGNWVTSSVTNLTGMFFNNTVFNQPIGNWDD256 pKa = 3.3 VSLVTNMQGVFNGATSFNQPLNNWNVSSVIVMDD289 pKa = 4.25 DD290 pKa = 3.06 MFRR293 pKa = 11.84 RR294 pKa = 11.84 ATSFNQPLNNWDD306 pKa = 3.33 VSNVVDD312 pKa = 4.06 MNLMFLSSAFNQPIGNWDD330 pKa = 3.44 VSSVTNMGGMFFNNTVFNQPIGSWDD355 pKa = 3.46 VSSVIDD361 pKa = 3.82 MNSMFNNATTFNQSLGAWNIGNLTSGVGFLDD392 pKa = 3.66 NSGLSLANWDD402 pKa = 3.63 ATLTGWDD409 pKa = 3.38 AQGFGNPNVTIGANGLQYY427 pKa = 10.86 CSAQTQRR434 pKa = 11.84 DD435 pKa = 3.5 AMQTAGIFTFVGDD448 pKa = 4.12 VLGCTPIITCGDD460 pKa = 3.59 AFNASSISKK469 pKa = 9.44 FSNRR473 pKa = 11.84 FEE475 pKa = 4.39 VTLHH479 pKa = 6.63 LDD481 pKa = 3.47 APTGLAFNTDD491 pKa = 3.38 GTKK494 pKa = 9.49 MFAIAEE500 pKa = 4.27 GLGGNDD506 pKa = 5.5 DD507 pKa = 5.37 IIAEE511 pKa = 4.31 YY512 pKa = 10.39 NLSIGFDD519 pKa = 3.42 TTTAVYY525 pKa = 10.43 AGAAEE530 pKa = 4.0 QFFVGGQEE538 pKa = 4.1 SDD540 pKa = 3.29 PHH542 pKa = 7.48 GLTFNAEE549 pKa = 3.97 GTKK552 pKa = 9.76 MFVIGLNGDD561 pKa = 3.57 AVVEE565 pKa = 4.3 YY566 pKa = 10.54 NLGVAFDD573 pKa = 3.86 VSTAIHH579 pKa = 6.55 AGAAEE584 pKa = 4.1 EE585 pKa = 4.36 FFVGGQEE592 pKa = 4.0 TSPTGLAFNTDD603 pKa = 3.42 GTKK606 pKa = 9.48 MFAVGVDD613 pKa = 3.47 DD614 pKa = 4.52 GAVVEE619 pKa = 4.79 YY620 pKa = 10.73 NLSTGFDD627 pKa = 3.34 VSTAVYY633 pKa = 10.09 AGASEE638 pKa = 4.21 EE639 pKa = 4.24 FFVGGQEE646 pKa = 4.2 SSPTGLTFNTDD657 pKa = 2.93 GTKK660 pKa = 9.66 MFVIGLNGDD669 pKa = 3.57 AVVEE673 pKa = 4.35 YY674 pKa = 10.32 NLSAGFDD681 pKa = 3.52 VSTAVYY687 pKa = 10.16 AGTSEE692 pKa = 4.1 EE693 pKa = 4.32 FSLLGYY699 pKa = 9.54 SASPVDD705 pKa = 5.64 LVFNTNGTKK714 pKa = 10.36 LYY716 pKa = 10.97 VLDD719 pKa = 4.36 GLFGGNYY726 pKa = 9.35 VLEE729 pKa = 4.62 FVQDD733 pKa = 3.04 VDD735 pKa = 3.99 NYY737 pKa = 11.24 HH738 pKa = 6.86 EE739 pKa = 4.34 ATATNGNIMANTKK752 pKa = 9.64 PLVYY756 pKa = 10.13 TIINDD761 pKa = 3.91 TFSDD765 pKa = 3.9 TNNDD769 pKa = 3.52 DD770 pKa = 3.78 VLDD773 pKa = 4.25 GGFTISNLPSGLTAEE788 pKa = 5.07 FVLSHH793 pKa = 6.83 GDD795 pKa = 3.23 TRR797 pKa = 11.84 ATLVLSGNAANNNSSNSIATLNIVFNATAVTSNTAPTLNCSSTTGIDD844 pKa = 4.17 FIDD847 pKa = 3.93 SAIVCANIFDD857 pKa = 4.05 VSTSEE862 pKa = 4.26 FSNQFSVNAQEE873 pKa = 3.93 NQLRR877 pKa = 11.84 GVEE880 pKa = 4.3 FNTDD884 pKa = 2.99 GTKK887 pKa = 9.63 MFVIGDD893 pKa = 3.63 SDD895 pKa = 4.06 DD896 pKa = 5.92 AIVEE900 pKa = 4.47 YY901 pKa = 10.86 NLSTGFDD908 pKa = 3.34 VSTAVYY914 pKa = 10.18 AGASQEE920 pKa = 4.19 FFFGGQVPNPLNFAFSTDD938 pKa = 3.21 GTKK941 pKa = 10.84 LFIINGIGPGGSSGLYY957 pKa = 9.63 QYY959 pKa = 11.88 NLGTGFDD966 pKa = 3.36 VSTAVFAGGFHH977 pKa = 7.0 RR978 pKa = 11.84 RR979 pKa = 11.84 FISQEE984 pKa = 3.9 SFPTDD989 pKa = 3.09 LAFNTDD995 pKa = 3.18 GSKK998 pKa = 9.73 MFIIGVSDD1006 pKa = 3.84 DD1007 pKa = 3.95 AVIEE1011 pKa = 4.33 YY1012 pKa = 10.28 ILRR1015 pKa = 11.84 ANFDD1019 pKa = 3.4 ISTMVYY1025 pKa = 10.12 AGAAEE1030 pKa = 4.26 EE1031 pKa = 4.26 FSVVAQEE1038 pKa = 4.7 SEE1040 pKa = 4.2 PTGLAFNTDD1049 pKa = 3.22 GTKK1052 pKa = 9.6 MFVIGNSGDD1061 pKa = 3.52 AVVEE1065 pKa = 4.38 YY1066 pKa = 10.74 NLSTGFDD1073 pKa = 3.31 VSTAVYY1079 pKa = 10.01 SGASEE1084 pKa = 4.26 EE1085 pKa = 4.41 FSVTTFEE1092 pKa = 5.14 AIPRR1096 pKa = 11.84 GLAFNANGSKK1106 pKa = 10.49 LYY1108 pKa = 10.91 VSGLVRR1114 pKa = 11.84 DD1115 pKa = 4.53 SIIEE1119 pKa = 4.09 FAVGEE1124 pKa = 3.98 ADD1126 pKa = 3.31 NYY1128 pKa = 11.02 EE1129 pKa = 4.03 EE1130 pKa = 5.09 ASINNGSIATNTSPFIYY1147 pKa = 10.1 NVSNDD1152 pKa = 2.97 TFADD1156 pKa = 3.73 VNNDD1160 pKa = 4.03 DD1161 pKa = 4.38 ILDD1164 pKa = 3.94 GGFTIGNLSPGLTPQFVLSNGDD1186 pKa = 3.25 TTATLVLSGNATNNSSSDD1204 pKa = 3.59 NIADD1208 pKa = 4.06 LNISFNTTAITSTTVPILDD1227 pKa = 4.36 CNTIVGIDD1235 pKa = 3.67 FLDD1238 pKa = 4.27 NINLNGRR1245 pKa = 11.84 LRR1247 pKa = 11.84 HH1248 pKa = 6.02 GKK1250 pKa = 9.64 FFLNGSEE1257 pKa = 4.03 QPMVTGRR1264 pKa = 11.84 NANN1267 pKa = 3.36
Molecular weight: 134.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.91
Patrickios 1.163
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A4S1DXL5|A0A4S1DXL5_9FLAO SRPBCC domain-containing protein OS=Flavivirga rizhaonensis OX=2559571 GN=EM932_08855 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.76 GRR40 pKa = 11.84 KK41 pKa = 7.91 RR42 pKa = 11.84 LSVSSEE48 pKa = 3.87 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.544
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.296
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.018
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4045
0
4045
1423285
23
4414
351.9
39.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.829 ± 0.035
0.778 ± 0.013
5.732 ± 0.03
6.31 ± 0.039
5.256 ± 0.029
6.266 ± 0.039
1.8 ± 0.016
8.377 ± 0.037
8.195 ± 0.056
9.168 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.005 ± 0.019
6.753 ± 0.043
3.289 ± 0.025
3.104 ± 0.021
3.303 ± 0.024
6.701 ± 0.028
5.881 ± 0.047
5.967 ± 0.025
1.083 ± 0.014
4.2 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here