Ferrimonas lipolytica
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H1UBZ8|A0A6H1UBZ8_9GAMM Adenine phosphoribosyltransferase OS=Ferrimonas lipolytica OX=2724191 GN=apt PE=3 SV=1
MM1 pKa = 7.19 KK2 pKa = 10.31 RR3 pKa = 11.84 SLVTLGVIVALAGCDD18 pKa = 3.48 SDD20 pKa = 3.54 NHH22 pKa = 5.83 NVVTSDD28 pKa = 3.34 FTLQLLHH35 pKa = 6.33 VADD38 pKa = 4.35 MDD40 pKa = 4.1 GSNATVLEE48 pKa = 4.29 NASNMSALVEE58 pKa = 4.42 GFTSQHH64 pKa = 5.92 ANNTLFLSSGDD75 pKa = 3.48 NYY77 pKa = 10.52 IPGSRR82 pKa = 11.84 YY83 pKa = 9.73 DD84 pKa = 3.45 AAADD88 pKa = 3.51 EE89 pKa = 4.86 SMVNVDD95 pKa = 4.07 TIGVPGVARR104 pKa = 11.84 ADD106 pKa = 3.25 IAMLNAMGLQASAVGNHH123 pKa = 6.6 DD124 pKa = 4.28 LDD126 pKa = 4.5 GGSAEE131 pKa = 4.2 FASIINTQSASSCDD145 pKa = 3.72 ANISCSADD153 pKa = 3.33 YY154 pKa = 10.73 IGAQFPYY161 pKa = 10.35 LSANMDD167 pKa = 3.74 FATDD171 pKa = 3.43 EE172 pKa = 4.06 NTIALVATAGQSAANLANKK191 pKa = 8.22 LTASTVITVDD201 pKa = 3.43 GEE203 pKa = 4.4 HH204 pKa = 6.86 IGIVGITTPTNDD216 pKa = 5.62 DD217 pKa = 3.3 ITTVDD222 pKa = 4.88 DD223 pKa = 3.67 IAVYY227 pKa = 9.76 PLNDD231 pKa = 5.57 DD232 pKa = 3.46 IGEE235 pKa = 4.14 LAAIVQSEE243 pKa = 4.57 VNALTATGINKK254 pKa = 9.75 IIALAHH260 pKa = 5.3 MQSITYY266 pKa = 8.2 EE267 pKa = 4.0 KK268 pKa = 10.54 QLAEE272 pKa = 4.39 LLRR275 pKa = 11.84 DD276 pKa = 3.15 VDD278 pKa = 4.21 IIVAGGSNTLLANDD292 pKa = 5.48 DD293 pKa = 3.68 DD294 pKa = 4.84 RR295 pKa = 11.84 LWAGDD300 pKa = 3.76 TAAGSYY306 pKa = 10.01 PIIKK310 pKa = 9.8 QDD312 pKa = 3.48 ADD314 pKa = 3.8 GNDD317 pKa = 3.36 VAIVNVDD324 pKa = 2.99 ADD326 pKa = 3.83 YY327 pKa = 10.97 KK328 pKa = 9.88 YY329 pKa = 10.82 LGRR332 pKa = 11.84 LVVDD336 pKa = 4.78 FDD338 pKa = 4.92 SDD340 pKa = 3.7 GKK342 pKa = 11.26 LLVDD346 pKa = 5.21 SISAADD352 pKa = 3.96 SGPVITDD359 pKa = 3.23 GQMVDD364 pKa = 3.3 ATEE367 pKa = 4.42 GATPNSDD374 pKa = 3.35 VIALVDD380 pKa = 4.33 SINEE384 pKa = 3.74 VLIEE388 pKa = 4.15 SEE390 pKa = 4.69 SNVVGHH396 pKa = 6.53 TDD398 pKa = 2.87 VFLNGTRR405 pKa = 11.84 SYY407 pKa = 11.63 VRR409 pKa = 11.84 TEE411 pKa = 3.66 EE412 pKa = 4.28 TNLGNLSADD421 pKa = 3.27 ANLWYY426 pKa = 10.34 AQLHH430 pKa = 6.14 DD431 pKa = 4.15 ASVVISLKK439 pKa = 10.61 NGGGIRR445 pKa = 11.84 ADD447 pKa = 2.97 IGYY450 pKa = 9.33 SAFPAGSTDD459 pKa = 3.85 PDD461 pKa = 3.99 DD462 pKa = 3.72 LTYY465 pKa = 10.65 YY466 pKa = 10.22 PPAAYY471 pKa = 8.25 PAAQKK476 pKa = 11.19 AEE478 pKa = 4.05 GDD480 pKa = 3.08 ISQYY484 pKa = 11.27 DD485 pKa = 3.79 VQTALAFNNGLSVFDD500 pKa = 3.94 LTGEE504 pKa = 4.1 QLWDD508 pKa = 3.3 ILEE511 pKa = 4.38 SGVAGVEE518 pKa = 4.14 NISGGFHH525 pKa = 6.31 HH526 pKa = 7.24 VAGLCFSYY534 pKa = 10.9 DD535 pKa = 3.18 GTKK538 pKa = 9.21 TARR541 pKa = 11.84 QSDD544 pKa = 4.49 PEE546 pKa = 4.4 SGAVTVAGEE555 pKa = 4.21 RR556 pKa = 11.84 VQQIIVDD563 pKa = 3.8 TDD565 pKa = 3.18 SDD567 pKa = 4.54 GNCDD571 pKa = 3.63 KK572 pKa = 11.4 NSDD575 pKa = 4.01 DD576 pKa = 3.69 MVMAGGTLQLPDD588 pKa = 3.09 ATYY591 pKa = 8.74 RR592 pKa = 11.84 TVSLDD597 pKa = 3.47 YY598 pKa = 10.34 LASGAPYY605 pKa = 9.6 PCAGDD610 pKa = 4.64 DD611 pKa = 5.0 CDD613 pKa = 4.58 NQVQLGDD620 pKa = 4.02 EE621 pKa = 4.36 MTTDD625 pKa = 3.76 PQASNFAATGSEE637 pKa = 3.54 QDD639 pKa = 3.49 ALAEE643 pKa = 3.98 FLQAFYY649 pKa = 11.22 ADD651 pKa = 4.16 SDD653 pKa = 4.01 SAYY656 pKa = 10.61 NSSDD660 pKa = 3.46 SVDD663 pKa = 3.36 GGEE666 pKa = 3.78 ADD668 pKa = 3.46 ARR670 pKa = 11.84 VIRR673 pKa = 11.84 LDD675 pKa = 3.3 KK676 pKa = 11.25
Molecular weight: 70.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.139
Thurlkill 3.541
EMBOSS 3.694
Sillero 3.846
Patrickios 1.24
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A6H1UGT7|A0A6H1UGT7_9GAMM GGDEF domain-containing protein OS=Ferrimonas lipolytica OX=2724191 GN=HER31_16195 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3539
0
3539
1156617
28
4188
326.8
36.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.778 ± 0.049
1.117 ± 0.016
5.637 ± 0.044
5.746 ± 0.035
3.719 ± 0.029
7.234 ± 0.04
2.32 ± 0.023
5.62 ± 0.032
4.224 ± 0.036
10.827 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.021
3.965 ± 0.029
4.142 ± 0.027
5.464 ± 0.056
4.915 ± 0.036
6.252 ± 0.038
5.254 ± 0.042
7.022 ± 0.031
1.309 ± 0.019
2.809 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here