Staphylococcus phage IME-SA4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D4DCC1|A0A0D4DCC1_9CAUD Putative membrane protein OS=Staphylococcus phage IME-SA4 OX=1610872 GN=IME_024 PE=4 SV=1
MM1 pKa = 7.95AYY3 pKa = 10.15SQIEE7 pKa = 4.33TTTDD11 pKa = 2.77EE12 pKa = 4.23EE13 pKa = 4.88MVVEE17 pKa = 5.09GYY19 pKa = 11.0AIIFDD24 pKa = 4.28TLSDD28 pKa = 3.73DD29 pKa = 3.86LGGFKK34 pKa = 10.43EE35 pKa = 4.61IISPTALSQVDD46 pKa = 3.93VTDD49 pKa = 3.85VKK51 pKa = 11.19CLINHH56 pKa = 7.7DD57 pKa = 4.05YY58 pKa = 10.29NQIVGRR64 pKa = 11.84TLANTLEE71 pKa = 4.23LTVDD75 pKa = 3.72DD76 pKa = 4.2KK77 pKa = 11.88GLYY80 pKa = 8.94FKK82 pKa = 11.0CFLPNTSYY90 pKa = 11.49ARR92 pKa = 11.84DD93 pKa = 3.19IYY95 pKa = 11.41EE96 pKa = 4.31NIKK99 pKa = 10.32AGNVNQCSFFYY110 pKa = 10.24TLPINDD116 pKa = 3.5DD117 pKa = 3.75TARR120 pKa = 11.84TWSKK124 pKa = 11.1INGEE128 pKa = 4.23YY129 pKa = 10.35VQTINTIDD137 pKa = 3.66EE138 pKa = 4.37LLEE141 pKa = 3.95VSIVTLPAYY150 pKa = 10.18RR151 pKa = 11.84DD152 pKa = 3.42TSVVVGQRR160 pKa = 11.84AKK162 pKa = 11.05GLDD165 pKa = 3.28KK166 pKa = 10.95FKK168 pKa = 10.9EE169 pKa = 4.02LEE171 pKa = 4.1KK172 pKa = 11.26YY173 pKa = 10.3KK174 pKa = 11.14LEE176 pKa = 4.42FDD178 pKa = 4.18LEE180 pKa = 4.29SLRR183 pKa = 11.84VDD185 pKa = 3.46TT186 pKa = 5.92

Molecular weight: 21.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D4DCX1|A0A0D4DCX1_9CAUD dUTP diphosphatase OS=Staphylococcus phage IME-SA4 OX=1610872 GN=IME_056 PE=4 SV=1
MM1 pKa = 7.43ILSDD5 pKa = 4.94TINQRR10 pKa = 11.84YY11 pKa = 9.1RR12 pKa = 11.84YY13 pKa = 7.45ATQGKK18 pKa = 7.42TPTQIQQEE26 pKa = 4.13LRR28 pKa = 11.84KK29 pKa = 9.72IGVNGFVVKK38 pKa = 10.52VAGSRR43 pKa = 11.84VTMKK47 pKa = 10.46VEE49 pKa = 3.88KK50 pKa = 10.76EE51 pKa = 4.0NIRR54 pKa = 11.84KK55 pKa = 8.63NRR57 pKa = 11.84EE58 pKa = 3.75CLRR61 pKa = 3.98

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12727

46

1795

205.3

23.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.752 ± 0.459

0.526 ± 0.12

6.38 ± 0.512

7.284 ± 0.555

4.102 ± 0.208

5.799 ± 0.483

1.674 ± 0.163

7.48 ± 0.211

9.389 ± 0.345

7.857 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.149

6.804 ± 0.34

2.624 ± 0.166

3.913 ± 0.199

4.463 ± 0.233

5.979 ± 0.293

6.294 ± 0.293

6.003 ± 0.201

1.186 ± 0.196

4.149 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski