Amino acid dipepetide frequency for Staphylococcus phage IME-SA4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.2AlaAla: 2.2 ± 0.45
0.314AlaCys: 0.314 ± 0.15
3.693AlaAsp: 3.693 ± 0.606
3.772AlaGlu: 3.772 ± 0.601
2.515AlaPhe: 2.515 ± 0.598
3.379AlaGly: 3.379 ± 0.63
1.1AlaHis: 1.1 ± 0.313
5.422AlaIle: 5.422 ± 1.1
5.815AlaLys: 5.815 ± 0.737
5.029AlaLeu: 5.029 ± 0.599
1.729AlaMet: 1.729 ± 0.295
3.3AlaAsn: 3.3 ± 0.504
1.257AlaPro: 1.257 ± 0.23
2.043AlaGln: 2.043 ± 0.446
2.907AlaArg: 2.907 ± 0.39
2.357AlaSer: 2.357 ± 0.447
3.693AlaThr: 3.693 ± 0.643
4.008AlaVal: 4.008 ± 0.574
0.707AlaTrp: 0.707 ± 0.187
2.279AlaTyr: 2.279 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.393CysAla: 0.393 ± 0.187
0.0CysCys: 0.0 ± 0.0
0.157CysAsp: 0.157 ± 0.12
0.55CysGlu: 0.55 ± 0.232
0.079CysPhe: 0.079 ± 0.079
0.393CysGly: 0.393 ± 0.161
0.157CysHis: 0.157 ± 0.113
0.314CysIle: 0.314 ± 0.184
0.314CysLys: 0.314 ± 0.149
0.393CysLeu: 0.393 ± 0.201
0.079CysMet: 0.079 ± 0.085
0.393CysAsn: 0.393 ± 0.144
0.079CysPro: 0.079 ± 0.09
0.236CysGln: 0.236 ± 0.13
0.236CysArg: 0.236 ± 0.124
0.471CysSer: 0.471 ± 0.299
0.314CysThr: 0.314 ± 0.137
0.314CysVal: 0.314 ± 0.144
0.079CysTrp: 0.079 ± 0.082
0.314CysTyr: 0.314 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
2.436AspAla: 2.436 ± 0.442
0.157AspCys: 0.157 ± 0.115
5.815AspAsp: 5.815 ± 0.928
6.365AspGlu: 6.365 ± 0.877
3.222AspPhe: 3.222 ± 0.597
3.222AspGly: 3.222 ± 0.503
0.707AspHis: 0.707 ± 0.226
5.186AspIle: 5.186 ± 0.7
6.522AspLys: 6.522 ± 0.607
5.108AspLeu: 5.108 ± 0.851
1.572AspMet: 1.572 ± 0.422
3.065AspAsn: 3.065 ± 0.661
1.022AspPro: 1.022 ± 0.266
1.022AspGln: 1.022 ± 0.268
2.122AspArg: 2.122 ± 0.355
3.379AspSer: 3.379 ± 0.455
4.243AspThr: 4.243 ± 0.662
4.558AspVal: 4.558 ± 0.87
0.786AspTrp: 0.786 ± 0.216
3.3AspTyr: 3.3 ± 0.546
0.0AspXaa: 0.0 ± 0.0
Glu
3.536GluAla: 3.536 ± 0.51
0.629GluCys: 0.629 ± 0.198
3.615GluAsp: 3.615 ± 0.485
5.972GluGlu: 5.972 ± 0.845
2.75GluPhe: 2.75 ± 0.442
2.436GluGly: 2.436 ± 0.363
1.257GluHis: 1.257 ± 0.357
5.893GluIle: 5.893 ± 0.704
5.815GluLys: 5.815 ± 0.68
8.801GluLeu: 8.801 ± 1.146
2.436GluMet: 2.436 ± 0.516
3.929GluAsn: 3.929 ± 0.681
1.493GluPro: 1.493 ± 0.328
3.536GluGln: 3.536 ± 0.483
3.379GluArg: 3.379 ± 0.495
2.907GluSer: 2.907 ± 0.422
4.008GluThr: 4.008 ± 0.496
5.265GluVal: 5.265 ± 0.682
1.179GluTrp: 1.179 ± 0.302
4.008GluTyr: 4.008 ± 0.622
0.0GluXaa: 0.0 ± 0.0
Phe
2.907PheAla: 2.907 ± 0.485
0.157PheCys: 0.157 ± 0.116
3.222PheAsp: 3.222 ± 0.584
2.75PheGlu: 2.75 ± 0.479
1.493PhePhe: 1.493 ± 0.354
2.986PheGly: 2.986 ± 0.47
0.393PheHis: 0.393 ± 0.154
3.85PheIle: 3.85 ± 0.703
3.457PheLys: 3.457 ± 0.5
2.357PheLeu: 2.357 ± 0.444
1.179PheMet: 1.179 ± 0.323
4.793PheAsn: 4.793 ± 0.877
0.55PhePro: 0.55 ± 0.193
0.629PheGln: 0.629 ± 0.19
1.886PheArg: 1.886 ± 0.329
2.593PheSer: 2.593 ± 0.419
1.414PheThr: 1.414 ± 0.346
2.122PheVal: 2.122 ± 0.411
0.157PheTrp: 0.157 ± 0.114
2.122PheTyr: 2.122 ± 0.499
0.0PheXaa: 0.0 ± 0.0
Gly
3.772GlyAla: 3.772 ± 1.016
0.079GlyCys: 0.079 ± 0.069
2.122GlyAsp: 2.122 ± 0.498
2.357GlyGlu: 2.357 ± 0.512
3.379GlyPhe: 3.379 ± 0.54
4.322GlyGly: 4.322 ± 0.756
1.022GlyHis: 1.022 ± 0.271
4.322GlyIle: 4.322 ± 0.71
5.265GlyLys: 5.265 ± 0.639
4.558GlyLeu: 4.558 ± 0.761
1.65GlyMet: 1.65 ± 0.546
3.85GlyAsn: 3.85 ± 0.515
0.707GlyPro: 0.707 ± 0.269
2.515GlyGln: 2.515 ± 0.466
2.593GlyArg: 2.593 ± 0.387
2.672GlySer: 2.672 ± 0.451
4.872GlyThr: 4.872 ± 0.576
3.929GlyVal: 3.929 ± 0.709
1.336GlyTrp: 1.336 ± 0.348
2.672GlyTyr: 2.672 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
0.707HisAla: 0.707 ± 0.24
0.079HisCys: 0.079 ± 0.069
0.707HisAsp: 0.707 ± 0.248
0.943HisGlu: 0.943 ± 0.324
1.1HisPhe: 1.1 ± 0.335
0.786HisGly: 0.786 ± 0.303
0.55HisHis: 0.55 ± 0.213
1.257HisIle: 1.257 ± 0.277
1.1HisLys: 1.1 ± 0.283
1.65HisLeu: 1.65 ± 0.435
0.393HisMet: 0.393 ± 0.174
1.1HisAsn: 1.1 ± 0.25
0.55HisPro: 0.55 ± 0.212
0.707HisGln: 0.707 ± 0.221
0.943HisArg: 0.943 ± 0.308
1.257HisSer: 1.257 ± 0.277
1.257HisThr: 1.257 ± 0.32
0.943HisVal: 0.943 ± 0.326
0.157HisTrp: 0.157 ± 0.098
0.55HisTyr: 0.55 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
5.422IleAla: 5.422 ± 0.62
0.629IleCys: 0.629 ± 0.195
5.265IleAsp: 5.265 ± 0.667
6.758IleGlu: 6.758 ± 0.916
3.536IlePhe: 3.536 ± 0.609
4.479IleGly: 4.479 ± 0.58
1.493IleHis: 1.493 ± 0.471
5.736IleIle: 5.736 ± 0.649
7.072IleLys: 7.072 ± 0.935
3.929IleLeu: 3.929 ± 0.573
1.257IleMet: 1.257 ± 0.298
5.186IleAsn: 5.186 ± 0.786
2.122IlePro: 2.122 ± 0.414
2.122IleGln: 2.122 ± 0.42
2.122IleArg: 2.122 ± 0.378
5.343IleSer: 5.343 ± 0.662
4.243IleThr: 4.243 ± 0.523
4.558IleVal: 4.558 ± 0.574
0.864IleTrp: 0.864 ± 0.247
2.672IleTyr: 2.672 ± 0.599
0.0IleXaa: 0.0 ± 0.0
Lys
4.715LysAla: 4.715 ± 0.579
0.314LysCys: 0.314 ± 0.213
5.579LysAsp: 5.579 ± 0.676
7.465LysGlu: 7.465 ± 1.081
2.986LysPhe: 2.986 ± 0.426
6.679LysGly: 6.679 ± 0.689
1.729LysHis: 1.729 ± 0.409
5.815LysIle: 5.815 ± 0.785
8.094LysLys: 8.094 ± 1.057
7.151LysLeu: 7.151 ± 0.749
1.729LysMet: 1.729 ± 0.473
7.544LysAsn: 7.544 ± 1.215
3.065LysPro: 3.065 ± 0.477
5.265LysGln: 5.265 ± 0.653
5.029LysArg: 5.029 ± 0.696
4.793LysSer: 4.793 ± 0.605
5.579LysThr: 5.579 ± 0.473
5.658LysVal: 5.658 ± 0.566
0.943LysTrp: 0.943 ± 0.19
3.772LysTyr: 3.772 ± 0.516
0.0LysXaa: 0.0 ± 0.0
Leu
5.265LeuAla: 5.265 ± 0.688
0.314LeuCys: 0.314 ± 0.156
4.95LeuAsp: 4.95 ± 0.765
6.758LeuGlu: 6.758 ± 0.818
3.379LeuPhe: 3.379 ± 0.427
3.615LeuGly: 3.615 ± 0.512
0.864LeuHis: 0.864 ± 0.244
5.029LeuIle: 5.029 ± 0.692
8.408LeuLys: 8.408 ± 0.804
5.736LeuLeu: 5.736 ± 0.701
1.964LeuMet: 1.964 ± 0.43
6.601LeuAsn: 6.601 ± 0.735
2.593LeuPro: 2.593 ± 0.524
2.593LeuGln: 2.593 ± 0.456
3.065LeuArg: 3.065 ± 0.453
5.658LeuSer: 5.658 ± 0.931
4.793LeuThr: 4.793 ± 0.591
3.929LeuVal: 3.929 ± 0.42
0.707LeuTrp: 0.707 ± 0.319
2.357LeuTyr: 2.357 ± 0.538
0.0LeuXaa: 0.0 ± 0.0
Met
2.122MetAla: 2.122 ± 0.408
0.079MetCys: 0.079 ± 0.083
1.179MetAsp: 1.179 ± 0.362
1.807MetGlu: 1.807 ± 0.486
1.022MetPhe: 1.022 ± 0.278
0.864MetGly: 0.864 ± 0.264
0.314MetHis: 0.314 ± 0.136
1.414MetIle: 1.414 ± 0.408
1.964MetLys: 1.964 ± 0.33
2.122MetLeu: 2.122 ± 0.459
0.314MetMet: 0.314 ± 0.136
1.257MetAsn: 1.257 ± 0.27
0.629MetPro: 0.629 ± 0.196
1.65MetGln: 1.65 ± 0.37
1.022MetArg: 1.022 ± 0.321
1.729MetSer: 1.729 ± 0.341
2.2MetThr: 2.2 ± 0.299
1.022MetVal: 1.022 ± 0.288
0.157MetTrp: 0.157 ± 0.104
0.55MetTyr: 0.55 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
4.793AsnAla: 4.793 ± 0.649
0.314AsnCys: 0.314 ± 0.168
4.086AsnAsp: 4.086 ± 0.63
4.715AsnGlu: 4.715 ± 0.616
1.964AsnPhe: 1.964 ± 0.36
5.658AsnGly: 5.658 ± 0.822
1.257AsnHis: 1.257 ± 0.317
4.636AsnIle: 4.636 ± 0.499
5.343AsnLys: 5.343 ± 0.696
5.422AsnLeu: 5.422 ± 0.708
1.179AsnMet: 1.179 ± 0.219
4.243AsnAsn: 4.243 ± 0.764
2.279AsnPro: 2.279 ± 0.369
2.986AsnGln: 2.986 ± 0.421
3.065AsnArg: 3.065 ± 0.601
5.108AsnSer: 5.108 ± 0.717
3.772AsnThr: 3.772 ± 0.637
4.165AsnVal: 4.165 ± 0.561
0.707AsnTrp: 0.707 ± 0.209
2.357AsnTyr: 2.357 ± 0.443
0.0AsnXaa: 0.0 ± 0.0
Pro
0.943ProAla: 0.943 ± 0.325
0.079ProCys: 0.079 ± 0.082
1.414ProAsp: 1.414 ± 0.32
2.357ProGlu: 2.357 ± 0.573
1.257ProPhe: 1.257 ± 0.348
0.786ProGly: 0.786 ± 0.252
0.314ProHis: 0.314 ± 0.159
2.357ProIle: 2.357 ± 0.388
2.829ProLys: 2.829 ± 0.49
2.2ProLeu: 2.2 ± 0.408
0.471ProMet: 0.471 ± 0.166
1.572ProAsn: 1.572 ± 0.349
1.022ProPro: 1.022 ± 0.391
0.629ProGln: 0.629 ± 0.224
0.786ProArg: 0.786 ± 0.26
1.493ProSer: 1.493 ± 0.376
2.672ProThr: 2.672 ± 0.434
1.886ProVal: 1.886 ± 0.349
0.0ProTrp: 0.0 ± 0.0
1.179ProTyr: 1.179 ± 0.278
0.0ProXaa: 0.0 ± 0.0
Gln
3.85GlnAla: 3.85 ± 0.6
0.236GlnCys: 0.236 ± 0.12
2.436GlnAsp: 2.436 ± 0.332
2.515GlnGlu: 2.515 ± 0.546
1.493GlnPhe: 1.493 ± 0.271
1.807GlnGly: 1.807 ± 0.357
0.786GlnHis: 0.786 ± 0.22
2.672GlnIle: 2.672 ± 0.454
2.357GlnLys: 2.357 ± 0.403
2.75GlnLeu: 2.75 ± 0.492
0.55GlnMet: 0.55 ± 0.201
3.065GlnAsn: 3.065 ± 0.547
1.336GlnPro: 1.336 ± 0.376
2.122GlnGln: 2.122 ± 0.537
1.964GlnArg: 1.964 ± 0.574
2.986GlnSer: 2.986 ± 0.405
2.043GlnThr: 2.043 ± 0.409
2.515GlnVal: 2.515 ± 0.435
0.157GlnTrp: 0.157 ± 0.115
1.493GlnTyr: 1.493 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
2.593ArgAla: 2.593 ± 0.496
0.236ArgCys: 0.236 ± 0.118
2.829ArgAsp: 2.829 ± 0.486
2.907ArgGlu: 2.907 ± 0.515
1.729ArgPhe: 1.729 ± 0.311
2.043ArgGly: 2.043 ± 0.348
0.864ArgHis: 0.864 ± 0.223
3.143ArgIle: 3.143 ± 0.43
4.95ArgLys: 4.95 ± 0.595
3.065ArgLeu: 3.065 ± 0.512
1.179ArgMet: 1.179 ± 0.366
2.907ArgAsn: 2.907 ± 0.562
0.943ArgPro: 0.943 ± 0.263
1.964ArgGln: 1.964 ± 0.368
2.2ArgArg: 2.2 ± 0.515
2.907ArgSer: 2.907 ± 0.485
2.515ArgThr: 2.515 ± 0.482
2.593ArgVal: 2.593 ± 0.36
0.629ArgTrp: 0.629 ± 0.207
2.357ArgTyr: 2.357 ± 0.499
0.0ArgXaa: 0.0 ± 0.0
Ser
2.829SerAla: 2.829 ± 0.693
0.157SerCys: 0.157 ± 0.126
5.029SerAsp: 5.029 ± 0.776
3.85SerGlu: 3.85 ± 0.547
2.593SerPhe: 2.593 ± 0.434
4.165SerGly: 4.165 ± 0.923
0.943SerHis: 0.943 ± 0.248
4.479SerIle: 4.479 ± 0.483
6.051SerLys: 6.051 ± 0.685
4.715SerLeu: 4.715 ± 0.676
1.572SerMet: 1.572 ± 0.356
3.929SerAsn: 3.929 ± 0.533
1.65SerPro: 1.65 ± 0.274
2.672SerGln: 2.672 ± 0.432
2.515SerArg: 2.515 ± 0.495
2.75SerSer: 2.75 ± 0.483
3.85SerThr: 3.85 ± 0.512
3.065SerVal: 3.065 ± 0.506
0.629SerTrp: 0.629 ± 0.245
2.357SerTyr: 2.357 ± 0.503
0.0SerXaa: 0.0 ± 0.0
Thr
3.3ThrAla: 3.3 ± 0.465
0.314ThrCys: 0.314 ± 0.141
4.008ThrAsp: 4.008 ± 0.666
3.222ThrGlu: 3.222 ± 0.426
2.436ThrPhe: 2.436 ± 0.373
4.636ThrGly: 4.636 ± 0.712
1.1ThrHis: 1.1 ± 0.299
4.95ThrIle: 4.95 ± 0.51
5.579ThrLys: 5.579 ± 0.61
5.343ThrLeu: 5.343 ± 0.914
1.257ThrMet: 1.257 ± 0.305
3.222ThrAsn: 3.222 ± 0.528
2.043ThrPro: 2.043 ± 0.286
3.222ThrGln: 3.222 ± 0.565
2.986ThrArg: 2.986 ± 0.557
3.85ThrSer: 3.85 ± 0.428
3.85ThrThr: 3.85 ± 0.651
3.85ThrVal: 3.85 ± 0.423
1.414ThrTrp: 1.414 ± 0.544
2.357ThrTyr: 2.357 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
3.222ValAla: 3.222 ± 0.494
0.236ValCys: 0.236 ± 0.144
4.4ValAsp: 4.4 ± 0.625
4.322ValGlu: 4.322 ± 0.79
2.043ValPhe: 2.043 ± 0.411
2.986ValGly: 2.986 ± 0.54
0.943ValHis: 0.943 ± 0.369
4.086ValIle: 4.086 ± 0.701
7.465ValLys: 7.465 ± 0.795
4.4ValLeu: 4.4 ± 0.586
1.179ValMet: 1.179 ± 0.314
3.85ValAsn: 3.85 ± 0.644
2.043ValPro: 2.043 ± 0.486
1.257ValGln: 1.257 ± 0.263
2.672ValArg: 2.672 ± 0.342
4.715ValSer: 4.715 ± 0.594
4.715ValThr: 4.715 ± 0.568
2.672ValVal: 2.672 ± 0.491
0.471ValTrp: 0.471 ± 0.205
2.357ValTyr: 2.357 ± 0.558
0.0ValXaa: 0.0 ± 0.0
Trp
0.629TrpAla: 0.629 ± 0.252
0.157TrpCys: 0.157 ± 0.104
0.864TrpAsp: 0.864 ± 0.265
0.629TrpGlu: 0.629 ± 0.166
0.471TrpPhe: 0.471 ± 0.185
0.629TrpGly: 0.629 ± 0.246
0.314TrpHis: 0.314 ± 0.193
0.864TrpIle: 0.864 ± 0.31
1.257TrpLys: 1.257 ± 0.26
0.786TrpLeu: 0.786 ± 0.257
0.236TrpMet: 0.236 ± 0.148
1.572TrpAsn: 1.572 ± 0.699
0.079TrpPro: 0.079 ± 0.085
0.393TrpGln: 0.393 ± 0.174
0.707TrpArg: 0.707 ± 0.269
0.707TrpSer: 0.707 ± 0.275
0.393TrpThr: 0.393 ± 0.161
0.471TrpVal: 0.471 ± 0.243
0.0TrpTrp: 0.0 ± 0.0
0.707TrpTyr: 0.707 ± 0.293
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.886TyrAla: 1.886 ± 0.376
0.786TyrCys: 0.786 ± 0.287
2.436TyrAsp: 2.436 ± 0.456
2.829TyrGlu: 2.829 ± 0.547
1.572TyrPhe: 1.572 ± 0.367
2.122TyrGly: 2.122 ± 0.441
0.629TyrHis: 0.629 ± 0.171
3.379TyrIle: 3.379 ± 0.68
4.243TyrLys: 4.243 ± 0.559
3.065TyrLeu: 3.065 ± 0.435
1.414TyrMet: 1.414 ± 0.325
2.672TyrAsn: 2.672 ± 0.44
0.786TyrPro: 0.786 ± 0.312
1.572TyrGln: 1.572 ± 0.309
2.436TyrArg: 2.436 ± 0.504
2.122TyrSer: 2.122 ± 0.424
2.515TyrThr: 2.515 ± 0.532
2.515TyrVal: 2.515 ± 0.543
0.786TyrTrp: 0.786 ± 0.249
1.729TyrTyr: 1.729 ± 0.44
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12727 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski