Ulvibacterium marinum
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5010 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B0C918|A0A3B0C918_9FLAO M23 family metallopeptidase OS=Ulvibacterium marinum OX=2419782 GN=D7Z94_02785 PE=4 SV=1
MM1 pKa = 7.0 YY2 pKa = 9.75 RR3 pKa = 11.84 YY4 pKa = 10.02 DD5 pKa = 5.62 AIDD8 pKa = 3.1 IMNFKK13 pKa = 9.96 VKK15 pKa = 10.52 VMFKK19 pKa = 10.46 RR20 pKa = 11.84 NTWGLIIWSTILLIGLGSCDD40 pKa = 3.5 SDD42 pKa = 4.08 DD43 pKa = 4.17 NAVADD48 pKa = 3.99 IPFTADD54 pKa = 2.48 VFNSVNGRR62 pKa = 11.84 QVAFQGITNNAVSWSWDD79 pKa = 3.35 FGDD82 pKa = 4.99 GNTSTSQNPVHH93 pKa = 7.09 RR94 pKa = 11.84 YY95 pKa = 10.09 AEE97 pKa = 4.13 GGYY100 pKa = 10.03 YY101 pKa = 10.11 VATLTATDD109 pKa = 3.89 EE110 pKa = 4.32 NGATLTSEE118 pKa = 4.06 VSLALDD124 pKa = 4.02 LPPYY128 pKa = 10.48 ALLVGNQTEE137 pKa = 4.0 EE138 pKa = 5.05 GYY140 pKa = 10.52 DD141 pKa = 3.31 GKK143 pKa = 8.55 TWRR146 pKa = 11.84 ISSEE150 pKa = 3.72 HH151 pKa = 6.14 SAFDD155 pKa = 3.46 YY156 pKa = 10.88 FAFSDD161 pKa = 3.77 ADD163 pKa = 3.58 LTLFDD168 pKa = 4.72 GTPAPLPAGIFGSGLGMGEE187 pKa = 4.02 IYY189 pKa = 10.6 NDD191 pKa = 3.06 EE192 pKa = 3.97 FTFYY196 pKa = 11.07 FDD198 pKa = 4.42 GSYY201 pKa = 11.45 KK202 pKa = 9.64 MDD204 pKa = 3.74 LKK206 pKa = 11.14 EE207 pKa = 5.32 DD208 pKa = 3.45 GTAFSGLVFQFLSFGGANIRR228 pKa = 11.84 NLGGEE233 pKa = 4.16 DD234 pKa = 4.22 FGLCTAAYY242 pKa = 8.15 TPAEE246 pKa = 4.3 DD247 pKa = 3.38 ATFTFTEE254 pKa = 4.44 SEE256 pKa = 4.21 DD257 pKa = 3.6 MTTTSAFGPGGMITYY272 pKa = 9.96 EE273 pKa = 4.26 GVTTLDD279 pKa = 3.35 FSGTEE284 pKa = 4.62 FVGFWDD290 pKa = 4.05 YY291 pKa = 11.42 EE292 pKa = 4.34 NKK294 pKa = 10.23 VILQDD299 pKa = 3.39 ITNSTMRR306 pKa = 11.84 LVVFVAASPDD316 pKa = 3.48 VPGINTNAIILTFEE330 pKa = 4.33 AVDD333 pKa = 3.37
Molecular weight: 36.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.567
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.91
Patrickios 1.214
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A3B0CAU5|A0A3B0CAU5_9FLAO Peptidase M14 OS=Ulvibacterium marinum OX=2419782 GN=D7Z94_09135 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.52 LSVSSEE47 pKa = 3.87 PRR49 pKa = 11.84 HH50 pKa = 5.92 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5010
0
5010
1768237
18
4831
352.9
39.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.358 ± 0.033
0.744 ± 0.011
5.759 ± 0.027
6.691 ± 0.032
5.194 ± 0.025
6.979 ± 0.035
1.942 ± 0.018
7.295 ± 0.029
7.068 ± 0.046
9.628 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.301 ± 0.016
5.549 ± 0.033
3.76 ± 0.022
3.379 ± 0.017
4.013 ± 0.022
6.341 ± 0.025
5.597 ± 0.038
6.194 ± 0.023
1.252 ± 0.013
3.957 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here