Capybara microvirus Cap3_SP_374
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8F7|A0A4P8W8F7_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_374 OX=2585437 PE=3 SV=1
MM1 pKa = 7.39 SKK3 pKa = 10.66 NVFIEE8 pKa = 4.27 RR9 pKa = 11.84 NDD11 pKa = 3.51 FEE13 pKa = 6.21 NDD15 pKa = 3.12 VKK17 pKa = 10.99 RR18 pKa = 11.84 SAFDD22 pKa = 3.84 LSHH25 pKa = 6.73 NNHH28 pKa = 6.7 LSLKK32 pKa = 9.33 LGKK35 pKa = 8.4 ITPVFLQEE43 pKa = 4.31 GLPGSTFKK51 pKa = 10.44 IDD53 pKa = 3.21 PVMALKK59 pKa = 10.19 AAPLVFPMQTKK70 pKa = 10.41 LKK72 pKa = 10.49 ASLSFFKK79 pKa = 11.28 VNTRR83 pKa = 11.84 TLWKK87 pKa = 10.11 DD88 pKa = 2.66 WTKK91 pKa = 10.82 FITNVEE97 pKa = 4.31 HH98 pKa = 7.63 DD99 pKa = 3.45 STMPKK104 pKa = 10.12 VSVSADD110 pKa = 3.3 SNFWKK115 pKa = 9.46 TGEE118 pKa = 4.04 LADD121 pKa = 3.86 YY122 pKa = 10.78 LGIPTAIYY130 pKa = 10.34 SSSEE134 pKa = 3.62 LTDD137 pKa = 3.2 YY138 pKa = 8.72 RR139 pKa = 11.84 TLHH142 pKa = 6.77 ALTEE146 pKa = 4.2 VQDD149 pKa = 5.01 LIKK152 pKa = 10.51 GWYY155 pKa = 8.63 LRR157 pKa = 11.84 YY158 pKa = 9.99 RR159 pKa = 11.84 EE160 pKa = 4.85 DD161 pKa = 4.67 DD162 pKa = 3.02 ASYY165 pKa = 10.51 WMKK168 pKa = 11.11 APINTTPSMNMKK180 pKa = 10.18 GYY182 pKa = 10.15 IGVAPSIDD190 pKa = 3.61 QTRR193 pKa = 11.84 SLYY196 pKa = 9.99 EE197 pKa = 3.67 QILVGRR203 pKa = 11.84 NDD205 pKa = 3.7 MVQQPYY211 pKa = 10.51 GFPAMSLTGYY221 pKa = 9.97 DD222 pKa = 3.89 GPGSSQQLTKK232 pKa = 10.62 SFPRR236 pKa = 11.84 ISRR239 pKa = 11.84 VFAITEE245 pKa = 3.87 QVKK248 pKa = 10.58 IGDD251 pKa = 3.96 VTCSFNSNTNTAVINFVRR269 pKa = 11.84 ADD271 pKa = 3.42 GTMLFYY277 pKa = 10.42 YY278 pKa = 9.88 TGTITAHH285 pKa = 7.03 KK286 pKa = 9.28 YY287 pKa = 10.61 SCTLTQANVNVINNEE302 pKa = 3.71 IANGSPVFVFAYY314 pKa = 8.86 TNQNFTTEE322 pKa = 4.59 ASFQPDD328 pKa = 3.16 TVEE331 pKa = 4.44 YY332 pKa = 10.12 IGEE335 pKa = 4.3 TLTVPVALTYY345 pKa = 10.61 TKK347 pKa = 10.85 DD348 pKa = 3.33 FTDD351 pKa = 3.04 ISEE354 pKa = 4.4 DD355 pKa = 3.49 PEE357 pKa = 4.19 RR358 pKa = 11.84 NPFANGSTQRR368 pKa = 11.84 NVVPISALPFRR379 pKa = 11.84 AYY381 pKa = 9.7 EE382 pKa = 3.96 AVYY385 pKa = 9.5 NAYY388 pKa = 9.96 YY389 pKa = 10.36 RR390 pKa = 11.84 NEE392 pKa = 4.11 RR393 pKa = 11.84 VDD395 pKa = 3.57 PLIINGRR402 pKa = 11.84 KK403 pKa = 9.68 EE404 pKa = 3.52 YY405 pKa = 10.8 DD406 pKa = 3.43 LYY408 pKa = 11.37 CNQEE412 pKa = 3.26 GGYY415 pKa = 10.7 DD416 pKa = 3.27 HH417 pKa = 7.38 TEE419 pKa = 4.12 YY420 pKa = 11.03 LLHH423 pKa = 6.86 DD424 pKa = 5.13 APWEE428 pKa = 4.03 TDD430 pKa = 3.18 VYY432 pKa = 9.55 TSCLPSPMDD441 pKa = 4.06 GPAPLIGLQSNLIGDD456 pKa = 5.14 KK457 pKa = 9.49 ITATMTTEE465 pKa = 3.54 SGEE468 pKa = 4.14 THH470 pKa = 7.28 DD471 pKa = 4.35 YY472 pKa = 11.09 EE473 pKa = 5.63 IGAVTDD479 pKa = 3.4 STGNITGFTYY489 pKa = 10.64 SEE491 pKa = 5.28 DD492 pKa = 3.2 IPYY495 pKa = 10.72 DD496 pKa = 3.41 LQRR499 pKa = 11.84 QLNQKK504 pKa = 8.47 VLSGISVNTIRR515 pKa = 11.84 NASAFEE521 pKa = 3.99 RR522 pKa = 11.84 YY523 pKa = 8.97 LRR525 pKa = 11.84 NGLRR529 pKa = 11.84 RR530 pKa = 11.84 GKK532 pKa = 10.51 KK533 pKa = 10.31 YY534 pKa = 9.79 IDD536 pKa = 3.22 NMKK539 pKa = 10.29 SHH541 pKa = 7.49 FGDD544 pKa = 3.12 THH546 pKa = 6.59 IQYY549 pKa = 10.98 DD550 pKa = 4.35 SLQMPTFHH558 pKa = 7.15 GGITSVFNVNQISSTTEE575 pKa = 3.34 NPAIDD580 pKa = 4.22 LALGSYY586 pKa = 9.48 AGQMSLIDD594 pKa = 4.28 NPNHH598 pKa = 6.54 TISIYY603 pKa = 10.65 CEE605 pKa = 3.68 EE606 pKa = 4.03 HH607 pKa = 6.85 CYY609 pKa = 10.36 IIGLLTVTPVPIYY622 pKa = 10.77 NQTLPKK628 pKa = 10.38 LFSKK632 pKa = 10.53 FDD634 pKa = 3.27 KK635 pKa = 10.76 FDD637 pKa = 4.15 FYY639 pKa = 11.23 TPEE642 pKa = 4.06 FDD644 pKa = 3.74 QLSMQPIKK652 pKa = 10.4 RR653 pKa = 11.84 QEE655 pKa = 3.81 VSVTNTYY662 pKa = 11.38 AEE664 pKa = 4.37 GGYY667 pKa = 6.66 TACNEE672 pKa = 4.1 TFGYY676 pKa = 8.96 QLPWYY681 pKa = 8.45 EE682 pKa = 4.29 YY683 pKa = 11.19 KK684 pKa = 10.72 NALDD688 pKa = 3.75 TTHH691 pKa = 6.31 GQMRR695 pKa = 11.84 TTMKK699 pKa = 10.11 DD700 pKa = 3.26 YY701 pKa = 11.04 NMNRR705 pKa = 11.84 VFHH708 pKa = 7.04 GIPQLSSDD716 pKa = 4.92 FIYY719 pKa = 10.96 CDD721 pKa = 3.73 GEE723 pKa = 4.14 TLNNVFAVADD733 pKa = 3.79 DD734 pKa = 4.37 TEE736 pKa = 5.0 DD737 pKa = 3.35 KK738 pKa = 11.38 VFGQIYY744 pKa = 9.95 FKK746 pKa = 8.93 TTVAHH751 pKa = 5.52 PMSRR755 pKa = 11.84 IGEE758 pKa = 4.48 PTWHH762 pKa = 6.71
Molecular weight: 86.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.917
IPC2_protein 5.041
IPC_protein 5.029
Toseland 5.003
ProMoST 5.169
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.952
Rodwell 4.965
Grimsley 4.94
Solomon 5.054
Lehninger 5.016
Nozaki 5.194
DTASelect 5.372
Thurlkill 5.029
EMBOSS 5.016
Sillero 5.258
Patrickios 3.325
IPC_peptide 5.067
IPC2_peptide 5.258
IPC2.peptide.svr19 5.161
Protein with the highest isoelectric point:
>tr|A0A4P8W8E6|A0A4P8W8E6_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_374 OX=2585437 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 5.59 CLNPVTLIIEE12 pKa = 4.27 KK13 pKa = 9.27 TVYY16 pKa = 10.49 NYY18 pKa = 9.05 EE19 pKa = 4.01 KK20 pKa = 9.91 QIKK23 pKa = 8.86 YY24 pKa = 10.72 AVIDD28 pKa = 3.57 NQRR31 pKa = 11.84 YY32 pKa = 9.78 DD33 pKa = 3.37 GLSAYY38 pKa = 10.18 DD39 pKa = 3.33 IIFYY43 pKa = 10.12 SRR45 pKa = 11.84 KK46 pKa = 9.78 ALEE49 pKa = 4.21 NRR51 pKa = 11.84 QSFPRR56 pKa = 11.84 DD57 pKa = 3.16 EE58 pKa = 4.17 QNRR61 pKa = 11.84 ILRR64 pKa = 11.84 YY65 pKa = 9.66 NPRR68 pKa = 11.84 EE69 pKa = 3.64 HH70 pKa = 6.13 QFYY73 pKa = 10.48 IRR75 pKa = 11.84 VEE77 pKa = 4.01 GVIDD81 pKa = 3.61 GRR83 pKa = 11.84 YY84 pKa = 7.39 KK85 pKa = 10.38 QKK87 pKa = 10.81 SIYY90 pKa = 8.92 FTQQVACRR98 pKa = 11.84 HH99 pKa = 5.69 CVLCVEE105 pKa = 5.12 RR106 pKa = 11.84 QRR108 pKa = 11.84 QDD110 pKa = 2.31 ITNRR114 pKa = 11.84 ILFEE118 pKa = 4.25 TEE120 pKa = 3.91 TYY122 pKa = 10.0 GPPIISTLTYY132 pKa = 10.66 NNQSLPTDD140 pKa = 3.72 GLNYY144 pKa = 10.46 SDD146 pKa = 3.63 VQKK149 pKa = 10.7 LFKK152 pKa = 10.59 VLRR155 pKa = 11.84 QTITRR160 pKa = 11.84 HH161 pKa = 4.72 SAIFTDD167 pKa = 4.95 GSGFRR172 pKa = 11.84 YY173 pKa = 9.37 FVAGEE178 pKa = 4.1 YY179 pKa = 11.16 GSITHH184 pKa = 7.05 RR185 pKa = 11.84 AHH187 pKa = 5.89 YY188 pKa = 10.12 HH189 pKa = 5.2 LLLWNFPTPQQCKK202 pKa = 6.59 EE203 pKa = 3.94 WYY205 pKa = 8.32 LKK207 pKa = 10.53 KK208 pKa = 10.98 YY209 pKa = 8.05 NTEE212 pKa = 3.56 LRR214 pKa = 11.84 VAYY217 pKa = 10.05 DD218 pKa = 3.53 PNPNNKK224 pKa = 8.29 NWYY227 pKa = 9.17 FPHH230 pKa = 7.04 LNNEE234 pKa = 4.13 YY235 pKa = 10.26 IIAEE239 pKa = 4.19 KK240 pKa = 10.22 KK241 pKa = 10.4 GRR243 pKa = 11.84 TFPVIEE249 pKa = 4.34 STSYY253 pKa = 10.9 LYY255 pKa = 10.1 QDD257 pKa = 3.38 YY258 pKa = 10.35 FIRR261 pKa = 11.84 YY262 pKa = 6.22 HH263 pKa = 6.04 WKK265 pKa = 9.99 RR266 pKa = 11.84 GLTLTEE272 pKa = 4.1 SMRR275 pKa = 11.84 GANTQSAATYY285 pKa = 8.3 CAKK288 pKa = 10.39 YY289 pKa = 9.8 VNKK292 pKa = 10.28 SASPHH297 pKa = 6.44 PNPNMNPTFYY307 pKa = 10.58 RR308 pKa = 11.84 SSTGNGGLGSRR319 pKa = 11.84 YY320 pKa = 7.66 IRR322 pKa = 11.84 EE323 pKa = 4.07 YY324 pKa = 9.5 IRR326 pKa = 11.84 PYY328 pKa = 10.64 ILTQYY333 pKa = 9.93 HH334 pKa = 5.72 ITGQVPLEE342 pKa = 4.02 YY343 pKa = 10.31 VYY345 pKa = 10.93 RR346 pKa = 11.84 PTNTTQIIHH355 pKa = 5.18 VPIRR359 pKa = 11.84 GYY361 pKa = 11.26 LLDD364 pKa = 3.4 QCFPTVSQLLPARR377 pKa = 11.84 YY378 pKa = 8.44 IEE380 pKa = 4.32 YY381 pKa = 8.84 YY382 pKa = 7.71 QTVIARR388 pKa = 11.84 SDD390 pKa = 3.18 TLQRR394 pKa = 11.84 LKK396 pKa = 11.26 VKK398 pKa = 10.59 DD399 pKa = 3.72 LAPNNIIRR407 pKa = 11.84 PMNTPRR413 pKa = 11.84 GNNLNEE419 pKa = 3.64 KK420 pKa = 9.49 EE421 pKa = 3.98 YY422 pKa = 11.02 RR423 pKa = 11.84 VFKK426 pKa = 10.76 RR427 pKa = 11.84 KK428 pKa = 9.79 LSLPEE433 pKa = 3.91 YY434 pKa = 10.8 NEE436 pKa = 3.98 MIQYY440 pKa = 10.87 LKK442 pKa = 10.92 DD443 pKa = 3.23 KK444 pKa = 11.25 SKK446 pKa = 11.19 DD447 pKa = 3.07 IDD449 pKa = 3.86 FKK451 pKa = 11.37 QVANMLQLRR460 pKa = 11.84 KK461 pKa = 9.66 IYY463 pKa = 10.4 LEE465 pKa = 3.98 NVAQQEE471 pKa = 4.16 LPYY474 pKa = 10.44 RR475 pKa = 11.84 DD476 pKa = 4.3 DD477 pKa = 5.83 LSDD480 pKa = 4.04 KK481 pKa = 10.43 SIKK484 pKa = 9.59 YY485 pKa = 8.71 EE486 pKa = 3.7 KK487 pKa = 10.43 LINFRR492 pKa = 11.84 KK493 pKa = 10.09 NRR495 pKa = 11.84 DD496 pKa = 3.48 KK497 pKa = 11.1 II498 pKa = 3.8
Molecular weight: 59.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.786
IPC2_protein 9.063
IPC_protein 9.004
Toseland 9.253
ProMoST 9.238
Dawson 9.633
Bjellqvist 9.458
Wikipedia 9.882
Rodwell 9.736
Grimsley 9.765
Solomon 9.663
Lehninger 9.589
Nozaki 9.37
DTASelect 9.414
Thurlkill 9.443
EMBOSS 9.721
Sillero 9.575
Patrickios 4.075
IPC_peptide 9.648
IPC2_peptide 8.244
IPC2.peptide.svr19 7.878
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1697
88
762
424.3
48.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.246 ± 1.458
0.943 ± 0.24
5.48 ± 0.524
5.716 ± 0.589
3.654 ± 0.986
5.127 ± 0.653
1.768 ± 0.419
6.364 ± 0.516
5.716 ± 0.711
7.778 ± 0.467
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.298 ± 0.432
7.307 ± 0.6
4.655 ± 0.728
6.011 ± 1.294
5.186 ± 1.143
6.011 ± 0.642
7.778 ± 1.153
4.891 ± 0.526
0.884 ± 0.108
6.187 ± 1.073
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here