Salmonella phage epsilon34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9UDI5|B9UDI5_9CAUD Uncharacterized protein 59 OS=Salmonella phage epsilon34 OX=348058 GN=59 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.61EE9 pKa = 3.97QEE11 pKa = 4.12SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 5.94VCEE35 pKa = 4.54HH36 pKa = 6.71CCAEE40 pKa = 5.41LMSDD44 pKa = 4.6PNNSMYY50 pKa = 10.74EE51 pKa = 4.03EE52 pKa = 4.59EE53 pKa = 5.1DD54 pKa = 3.95DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9UDI4|B9UDI4_9CAUD NinF OS=Salmonella phage epsilon34 OX=348058 GN=58 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.48RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.55PKK29 pKa = 10.29PIPKK33 pKa = 9.96EE34 pKa = 3.93SDD36 pKa = 3.19VKK38 pKa = 9.6TFNYY42 pKa = 7.51TAHH45 pKa = 6.73LWDD48 pKa = 3.61IRR50 pKa = 11.84WLRR53 pKa = 11.84EE54 pKa = 3.55RR55 pKa = 11.84ARR57 pKa = 11.84KK58 pKa = 7.22TRR60 pKa = 3.32

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13730

26

725

188.1

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.235 ± 0.608

1.224 ± 0.169

5.929 ± 0.256

6.373 ± 0.398

3.86 ± 0.26

7.298 ± 0.502

1.916 ± 0.185

6.118 ± 0.368

6.111 ± 0.324

7.597 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.136

4.72 ± 0.212

3.948 ± 0.181

4.683 ± 0.379

5.892 ± 0.314

6.344 ± 0.337

5.062 ± 0.252

5.958 ± 0.241

1.602 ± 0.157

3.336 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski