Amino acid dipepetide frequency for Salmonella phage epsilon34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.959AlaAla: 8.959 ± 1.59
1.093AlaCys: 1.093 ± 0.366
6.41AlaAsp: 6.41 ± 0.851
6.92AlaGlu: 6.92 ± 0.772
3.423AlaPhe: 3.423 ± 0.557
8.304AlaGly: 8.304 ± 1.355
1.093AlaHis: 1.093 ± 0.325
5.39AlaIle: 5.39 ± 0.87
5.39AlaLys: 5.39 ± 0.628
6.774AlaLeu: 6.774 ± 0.897
2.768AlaMet: 2.768 ± 0.526
4.953AlaAsn: 4.953 ± 0.819
2.549AlaPro: 2.549 ± 0.498
3.205AlaGln: 3.205 ± 0.551
5.172AlaArg: 5.172 ± 0.597
5.244AlaSer: 5.244 ± 0.524
5.099AlaThr: 5.099 ± 0.764
5.9AlaVal: 5.9 ± 0.725
1.311AlaTrp: 1.311 ± 0.271
2.404AlaTyr: 2.404 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
1.238CysAla: 1.238 ± 0.325
0.219CysCys: 0.219 ± 0.122
0.51CysAsp: 0.51 ± 0.198
0.874CysGlu: 0.874 ± 0.278
0.51CysPhe: 0.51 ± 0.202
1.238CysGly: 1.238 ± 0.307
0.583CysHis: 0.583 ± 0.232
0.656CysIle: 0.656 ± 0.229
0.801CysLys: 0.801 ± 0.225
0.874CysLeu: 0.874 ± 0.27
0.219CysMet: 0.219 ± 0.119
0.291CysAsn: 0.291 ± 0.141
0.219CysPro: 0.219 ± 0.124
0.219CysGln: 0.219 ± 0.119
1.093CysArg: 1.093 ± 0.325
0.728CysSer: 0.728 ± 0.295
0.51CysThr: 0.51 ± 0.214
0.801CysVal: 0.801 ± 0.225
0.146CysTrp: 0.146 ± 0.118
0.51CysTyr: 0.51 ± 0.188
0.0CysXaa: 0.0 ± 0.0
Asp
6.992AspAla: 6.992 ± 0.912
0.51AspCys: 0.51 ± 0.19
4.589AspAsp: 4.589 ± 0.632
3.423AspGlu: 3.423 ± 0.476
2.404AspPhe: 2.404 ± 0.368
5.099AspGly: 5.099 ± 0.689
1.165AspHis: 1.165 ± 0.287
4.152AspIle: 4.152 ± 0.532
3.278AspLys: 3.278 ± 0.506
5.172AspLeu: 5.172 ± 0.839
1.894AspMet: 1.894 ± 0.386
1.821AspAsn: 1.821 ± 0.301
2.039AspPro: 2.039 ± 0.457
1.384AspGln: 1.384 ± 0.29
2.549AspArg: 2.549 ± 0.406
3.278AspSer: 3.278 ± 0.488
1.457AspThr: 1.457 ± 0.301
4.807AspVal: 4.807 ± 0.604
1.02AspTrp: 1.02 ± 0.328
2.258AspTyr: 2.258 ± 0.411
0.0AspXaa: 0.0 ± 0.0
Glu
5.026GluAla: 5.026 ± 0.634
1.02GluCys: 1.02 ± 0.246
2.914GluAsp: 2.914 ± 0.48
4.152GluGlu: 4.152 ± 0.732
2.185GluPhe: 2.185 ± 0.401
4.006GluGly: 4.006 ± 0.495
1.238GluHis: 1.238 ± 0.309
4.807GluIle: 4.807 ± 0.619
3.569GluLys: 3.569 ± 0.533
6.483GluLeu: 6.483 ± 0.739
1.967GluMet: 1.967 ± 0.357
2.695GluAsn: 2.695 ± 0.572
2.112GluPro: 2.112 ± 0.376
3.933GluGln: 3.933 ± 0.509
4.152GluArg: 4.152 ± 0.593
3.205GluSer: 3.205 ± 0.454
2.841GluThr: 2.841 ± 0.549
3.788GluVal: 3.788 ± 0.678
1.748GluTrp: 1.748 ± 0.371
1.894GluTyr: 1.894 ± 0.408
0.0GluXaa: 0.0 ± 0.0
Phe
2.549PheAla: 2.549 ± 0.405
0.583PheCys: 0.583 ± 0.279
2.695PheAsp: 2.695 ± 0.423
1.967PheGlu: 1.967 ± 0.36
1.967PhePhe: 1.967 ± 0.552
2.768PheGly: 2.768 ± 0.421
0.728PheHis: 0.728 ± 0.24
2.622PheIle: 2.622 ± 0.588
2.258PheLys: 2.258 ± 0.387
1.894PheLeu: 1.894 ± 0.401
0.947PheMet: 0.947 ± 0.199
2.404PheAsn: 2.404 ± 0.339
1.384PhePro: 1.384 ± 0.289
1.311PheGln: 1.311 ± 0.398
1.748PheArg: 1.748 ± 0.431
3.496PheSer: 3.496 ± 0.536
2.549PheThr: 2.549 ± 0.483
2.112PheVal: 2.112 ± 0.341
0.947PheTrp: 0.947 ± 0.234
1.675PheTyr: 1.675 ± 0.428
0.0PheXaa: 0.0 ± 0.0
Gly
6.628GlyAla: 6.628 ± 1.107
0.801GlyCys: 0.801 ± 0.233
3.788GlyAsp: 3.788 ± 0.599
4.589GlyGlu: 4.589 ± 0.596
3.205GlyPhe: 3.205 ± 0.593
5.172GlyGly: 5.172 ± 0.949
1.165GlyHis: 1.165 ± 0.343
4.953GlyIle: 4.953 ± 0.858
4.88GlyLys: 4.88 ± 0.608
4.225GlyLeu: 4.225 ± 0.591
2.404GlyMet: 2.404 ± 0.436
3.715GlyAsn: 3.715 ± 0.442
1.384GlyPro: 1.384 ± 0.288
4.37GlyGln: 4.37 ± 0.788
5.026GlyArg: 5.026 ± 0.673
3.569GlySer: 3.569 ± 0.583
3.715GlyThr: 3.715 ± 0.542
5.609GlyVal: 5.609 ± 0.585
1.675GlyTrp: 1.675 ± 0.325
2.112GlyTyr: 2.112 ± 0.427
0.0GlyXaa: 0.0 ± 0.0
His
1.093HisAla: 1.093 ± 0.288
0.437HisCys: 0.437 ± 0.189
1.093HisAsp: 1.093 ± 0.311
1.675HisGlu: 1.675 ± 0.366
1.02HisPhe: 1.02 ± 0.297
1.748HisGly: 1.748 ± 0.368
0.51HisHis: 0.51 ± 0.19
0.728HisIle: 0.728 ± 0.246
1.093HisLys: 1.093 ± 0.312
1.821HisLeu: 1.821 ± 0.424
0.437HisMet: 0.437 ± 0.161
0.728HisAsn: 0.728 ± 0.202
0.874HisPro: 0.874 ± 0.25
0.874HisGln: 0.874 ± 0.253
1.093HisArg: 1.093 ± 0.276
1.457HisSer: 1.457 ± 0.392
0.656HisThr: 0.656 ± 0.24
0.801HisVal: 0.801 ± 0.231
0.291HisTrp: 0.291 ± 0.177
0.728HisTyr: 0.728 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
6.701IleAla: 6.701 ± 0.799
0.656IleCys: 0.656 ± 0.231
4.297IleAsp: 4.297 ± 0.598
3.205IleGlu: 3.205 ± 0.442
1.675IlePhe: 1.675 ± 0.393
4.589IleGly: 4.589 ± 0.595
1.53IleHis: 1.53 ± 0.337
3.788IleIle: 3.788 ± 0.747
3.132IleLys: 3.132 ± 0.585
3.642IleLeu: 3.642 ± 0.717
1.02IleMet: 1.02 ± 0.285
3.423IleAsn: 3.423 ± 0.577
2.841IlePro: 2.841 ± 0.529
2.768IleGln: 2.768 ± 0.394
3.423IleArg: 3.423 ± 0.425
4.443IleSer: 4.443 ± 0.554
4.297IleThr: 4.297 ± 0.519
2.841IleVal: 2.841 ± 0.348
1.02IleTrp: 1.02 ± 0.276
1.894IleTyr: 1.894 ± 0.507
0.0IleXaa: 0.0 ± 0.0
Lys
5.244LysAla: 5.244 ± 0.818
0.801LysCys: 0.801 ± 0.207
3.059LysAsp: 3.059 ± 0.42
5.099LysGlu: 5.099 ± 0.703
2.331LysPhe: 2.331 ± 0.362
4.37LysGly: 4.37 ± 0.572
1.238LysHis: 1.238 ± 0.368
2.986LysIle: 2.986 ± 0.601
4.152LysLys: 4.152 ± 0.635
5.536LysLeu: 5.536 ± 0.855
1.675LysMet: 1.675 ± 0.359
1.748LysAsn: 1.748 ± 0.36
2.914LysPro: 2.914 ± 0.48
3.496LysGln: 3.496 ± 0.579
4.152LysArg: 4.152 ± 0.561
4.006LysSer: 4.006 ± 0.546
3.059LysThr: 3.059 ± 0.524
2.768LysVal: 2.768 ± 0.459
0.583LysTrp: 0.583 ± 0.215
1.894LysTyr: 1.894 ± 0.391
0.0LysXaa: 0.0 ± 0.0
Leu
6.92LeuAla: 6.92 ± 0.821
0.874LeuCys: 0.874 ± 0.294
3.642LeuAsp: 3.642 ± 0.558
4.953LeuGlu: 4.953 ± 0.738
3.351LeuPhe: 3.351 ± 0.614
4.006LeuGly: 4.006 ± 0.585
1.311LeuHis: 1.311 ± 0.286
4.297LeuIle: 4.297 ± 0.729
5.172LeuLys: 5.172 ± 0.65
5.39LeuLeu: 5.39 ± 0.931
1.967LeuMet: 1.967 ± 0.377
4.735LeuAsn: 4.735 ± 0.562
3.278LeuPro: 3.278 ± 0.446
3.132LeuGln: 3.132 ± 0.504
5.026LeuArg: 5.026 ± 0.777
5.609LeuSer: 5.609 ± 0.629
4.225LeuThr: 4.225 ± 0.464
4.662LeuVal: 4.662 ± 0.559
1.384LeuTrp: 1.384 ± 0.334
2.039LeuTyr: 2.039 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
3.496MetAla: 3.496 ± 0.492
0.291MetCys: 0.291 ± 0.148
1.748MetAsp: 1.748 ± 0.284
1.238MetGlu: 1.238 ± 0.292
0.51MetPhe: 0.51 ± 0.177
1.748MetGly: 1.748 ± 0.336
0.364MetHis: 0.364 ± 0.164
1.602MetIle: 1.602 ± 0.389
2.185MetLys: 2.185 ± 0.371
2.404MetLeu: 2.404 ± 0.435
0.656MetMet: 0.656 ± 0.206
1.238MetAsn: 1.238 ± 0.276
1.53MetPro: 1.53 ± 0.289
1.238MetGln: 1.238 ± 0.379
1.894MetArg: 1.894 ± 0.408
2.039MetSer: 2.039 ± 0.39
1.53MetThr: 1.53 ± 0.336
1.093MetVal: 1.093 ± 0.248
0.073MetTrp: 0.073 ± 0.07
1.093MetTyr: 1.093 ± 0.316
0.0MetXaa: 0.0 ± 0.0
Asn
4.953AsnAla: 4.953 ± 0.68
0.437AsnCys: 0.437 ± 0.179
2.477AsnAsp: 2.477 ± 0.443
2.986AsnGlu: 2.986 ± 0.36
0.801AsnPhe: 0.801 ± 0.231
4.37AsnGly: 4.37 ± 0.515
1.238AsnHis: 1.238 ± 0.322
2.768AsnIle: 2.768 ± 0.44
2.914AsnLys: 2.914 ± 0.413
3.059AsnLeu: 3.059 ± 0.478
1.384AsnMet: 1.384 ± 0.294
2.039AsnAsn: 2.039 ± 0.466
2.841AsnPro: 2.841 ± 0.402
2.768AsnGln: 2.768 ± 0.557
2.404AsnArg: 2.404 ± 0.389
2.622AsnSer: 2.622 ± 0.345
2.185AsnThr: 2.185 ± 0.458
2.622AsnVal: 2.622 ± 0.656
0.437AsnTrp: 0.437 ± 0.177
1.894AsnTyr: 1.894 ± 0.425
0.0AsnXaa: 0.0 ± 0.0
Pro
2.914ProAla: 2.914 ± 0.386
0.437ProCys: 0.437 ± 0.171
2.768ProAsp: 2.768 ± 0.399
4.443ProGlu: 4.443 ± 0.552
1.675ProPhe: 1.675 ± 0.328
1.821ProGly: 1.821 ± 0.287
0.437ProHis: 0.437 ± 0.212
2.477ProIle: 2.477 ± 0.449
2.914ProLys: 2.914 ± 0.474
1.894ProLeu: 1.894 ± 0.326
0.728ProMet: 0.728 ± 0.234
1.748ProAsn: 1.748 ± 0.324
1.675ProPro: 1.675 ± 0.378
1.675ProGln: 1.675 ± 0.362
1.457ProArg: 1.457 ± 0.305
3.351ProSer: 3.351 ± 0.477
2.258ProThr: 2.258 ± 0.427
2.986ProVal: 2.986 ± 0.448
0.364ProTrp: 0.364 ± 0.164
1.457ProTyr: 1.457 ± 0.353
0.0ProXaa: 0.0 ± 0.0
Gln
4.225GlnAla: 4.225 ± 0.807
0.51GlnCys: 0.51 ± 0.226
2.841GlnAsp: 2.841 ± 0.517
2.477GlnGlu: 2.477 ± 0.472
1.53GlnPhe: 1.53 ± 0.344
2.914GlnGly: 2.914 ± 0.638
0.874GlnHis: 0.874 ± 0.219
3.278GlnIle: 3.278 ± 0.504
2.258GlnLys: 2.258 ± 0.397
3.788GlnLeu: 3.788 ± 0.598
1.53GlnMet: 1.53 ± 0.346
2.695GlnAsn: 2.695 ± 0.679
1.602GlnPro: 1.602 ± 0.319
3.351GlnGln: 3.351 ± 0.755
2.331GlnArg: 2.331 ± 0.378
3.423GlnSer: 3.423 ± 0.506
1.821GlnThr: 1.821 ± 0.425
2.331GlnVal: 2.331 ± 0.416
0.947GlnTrp: 0.947 ± 0.241
2.112GlnTyr: 2.112 ± 0.394
0.0GlnXaa: 0.0 ± 0.0
Arg
5.172ArgAla: 5.172 ± 0.58
1.238ArgCys: 1.238 ± 0.293
4.443ArgAsp: 4.443 ± 0.575
4.006ArgGlu: 4.006 ± 0.631
1.748ArgPhe: 1.748 ± 0.33
3.132ArgGly: 3.132 ± 0.423
1.457ArgHis: 1.457 ± 0.334
3.351ArgIle: 3.351 ± 0.426
3.715ArgLys: 3.715 ± 0.705
5.536ArgLeu: 5.536 ± 0.707
2.185ArgMet: 2.185 ± 0.404
2.549ArgAsn: 2.549 ± 0.291
1.894ArgPro: 1.894 ± 0.335
2.914ArgGln: 2.914 ± 0.522
3.059ArgArg: 3.059 ± 0.493
3.351ArgSer: 3.351 ± 0.475
2.622ArgThr: 2.622 ± 0.326
3.569ArgVal: 3.569 ± 0.415
0.656ArgTrp: 0.656 ± 0.24
2.331ArgTyr: 2.331 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
6.118SerAla: 6.118 ± 1.043
0.146SerCys: 0.146 ± 0.083
3.351SerAsp: 3.351 ± 0.447
3.278SerGlu: 3.278 ± 0.563
2.841SerPhe: 2.841 ± 0.638
5.973SerGly: 5.973 ± 0.718
1.238SerHis: 1.238 ± 0.373
4.152SerIle: 4.152 ± 0.974
3.569SerLys: 3.569 ± 0.494
5.172SerLeu: 5.172 ± 0.621
2.404SerMet: 2.404 ± 0.395
2.549SerAsn: 2.549 ± 0.369
3.205SerPro: 3.205 ± 0.611
2.768SerGln: 2.768 ± 0.424
3.788SerArg: 3.788 ± 0.535
3.278SerSer: 3.278 ± 0.553
2.986SerThr: 2.986 ± 0.485
2.986SerVal: 2.986 ± 0.392
1.165SerTrp: 1.165 ± 0.301
2.477SerTyr: 2.477 ± 0.379
0.0SerXaa: 0.0 ± 0.0
Thr
4.807ThrAla: 4.807 ± 0.517
0.51ThrCys: 0.51 ± 0.181
3.059ThrAsp: 3.059 ± 0.555
2.549ThrGlu: 2.549 ± 0.464
2.185ThrPhe: 2.185 ± 0.447
5.244ThrGly: 5.244 ± 0.62
0.801ThrHis: 0.801 ± 0.211
2.986ThrIle: 2.986 ± 0.498
3.496ThrLys: 3.496 ± 0.451
3.423ThrLeu: 3.423 ± 0.467
1.311ThrMet: 1.311 ± 0.27
2.112ThrAsn: 2.112 ± 0.376
2.914ThrPro: 2.914 ± 0.45
2.404ThrGln: 2.404 ± 0.32
2.695ThrArg: 2.695 ± 0.53
3.351ThrSer: 3.351 ± 0.567
2.258ThrThr: 2.258 ± 0.349
2.695ThrVal: 2.695 ± 0.414
0.437ThrTrp: 0.437 ± 0.155
1.384ThrTyr: 1.384 ± 0.321
0.0ThrXaa: 0.0 ± 0.0
Val
5.536ValAla: 5.536 ± 0.614
0.801ValCys: 0.801 ± 0.215
2.695ValAsp: 2.695 ± 0.32
3.715ValGlu: 3.715 ± 0.67
2.841ValPhe: 2.841 ± 0.504
3.569ValGly: 3.569 ± 0.593
0.947ValHis: 0.947 ± 0.312
3.642ValIle: 3.642 ± 0.519
3.788ValLys: 3.788 ± 0.559
3.933ValLeu: 3.933 ± 0.492
1.238ValMet: 1.238 ± 0.314
3.788ValAsn: 3.788 ± 0.542
2.404ValPro: 2.404 ± 0.401
2.258ValGln: 2.258 ± 0.389
3.569ValArg: 3.569 ± 0.422
3.788ValSer: 3.788 ± 0.435
4.225ValThr: 4.225 ± 0.565
4.006ValVal: 4.006 ± 0.589
0.874ValTrp: 0.874 ± 0.275
1.967ValTyr: 1.967 ± 0.505
0.0ValXaa: 0.0 ± 0.0
Trp
0.947TrpAla: 0.947 ± 0.266
0.291TrpCys: 0.291 ± 0.131
0.801TrpAsp: 0.801 ± 0.278
0.583TrpGlu: 0.583 ± 0.223
0.656TrpPhe: 0.656 ± 0.215
0.801TrpGly: 0.801 ± 0.213
0.437TrpHis: 0.437 ± 0.209
0.583TrpIle: 0.583 ± 0.202
0.947TrpLys: 0.947 ± 0.325
2.258TrpLeu: 2.258 ± 0.506
0.437TrpMet: 0.437 ± 0.154
0.656TrpAsn: 0.656 ± 0.219
0.583TrpPro: 0.583 ± 0.194
1.093TrpGln: 1.093 ± 0.27
1.457TrpArg: 1.457 ± 0.288
0.874TrpSer: 0.874 ± 0.249
0.583TrpThr: 0.583 ± 0.186
1.165TrpVal: 1.165 ± 0.266
0.437TrpTrp: 0.437 ± 0.19
0.437TrpTyr: 0.437 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.841TyrAla: 2.841 ± 0.406
0.583TyrCys: 0.583 ± 0.198
2.112TyrAsp: 2.112 ± 0.438
1.602TyrGlu: 1.602 ± 0.442
1.748TyrPhe: 1.748 ± 0.407
2.112TyrGly: 2.112 ± 0.504
0.801TyrHis: 0.801 ± 0.237
1.967TyrIle: 1.967 ± 0.427
1.602TyrLys: 1.602 ± 0.253
2.695TyrLeu: 2.695 ± 0.523
0.728TyrMet: 0.728 ± 0.232
1.311TyrAsn: 1.311 ± 0.385
1.238TyrPro: 1.238 ± 0.409
1.675TyrGln: 1.675 ± 0.328
2.841TyrArg: 2.841 ± 0.493
2.331TyrSer: 2.331 ± 0.449
1.748TyrThr: 1.748 ± 0.34
2.039TyrVal: 2.039 ± 0.393
0.51TyrTrp: 0.51 ± 0.214
0.801TyrTyr: 0.801 ± 0.259
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (13730 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski