Vibrio metschnikovii CIP 69.14

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio metschnikovii

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9P976|C9P976_VIBME Peptidyl-prolyl cis-trans isomerase OS=Vibrio metschnikovii CIP 69.14 OX=675813 GN=VIB_003071 PE=3 SV=1
MM1 pKa = 7.53GGAEE5 pKa = 4.0YY6 pKa = 10.83VGDD9 pKa = 4.8HH10 pKa = 6.68ISDD13 pKa = 3.79VLQQAGLDD21 pKa = 3.94TQIHH25 pKa = 5.37NQPVLADD32 pKa = 3.84IPAQGTWLIVTSTHH46 pKa = 6.86GAGDD50 pKa = 4.05YY51 pKa = 10.2PDD53 pKa = 5.25NIQPFINDD61 pKa = 3.91LQQTPPNTQQLSYY74 pKa = 10.83AVVAIGDD81 pKa = 3.64SSYY84 pKa = 11.09DD85 pKa = 3.6TFCAAGKK92 pKa = 9.45HH93 pKa = 6.12ADD95 pKa = 4.89DD96 pKa = 4.52LLEE99 pKa = 5.77DD100 pKa = 3.81IGATPIADD108 pKa = 4.29CLTIDD113 pKa = 3.89IQHH116 pKa = 6.57HH117 pKa = 6.08PVPEE121 pKa = 4.34DD122 pKa = 3.82AVEE125 pKa = 3.98EE126 pKa = 4.19WLSEE130 pKa = 4.24FIQRR134 pKa = 11.84VAKK137 pKa = 10.53

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9P941|C9P941_VIBME Methionyl-tRNA formyltransferase OS=Vibrio metschnikovii CIP 69.14 OX=675813 GN=fmt PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.42RR13 pKa = 11.84KK14 pKa = 9.36RR15 pKa = 11.84SHH17 pKa = 6.15GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 9.34VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.74GRR40 pKa = 11.84KK41 pKa = 8.91RR42 pKa = 11.84LSKK45 pKa = 10.84

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3034

0

3034

1011234

37

2822

333.3

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.963 ± 0.043

1.029 ± 0.014

5.241 ± 0.039

5.884 ± 0.045

3.994 ± 0.037

6.73 ± 0.045

2.511 ± 0.024

6.517 ± 0.04

4.664 ± 0.035

10.797 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.021

3.938 ± 0.031

4.024 ± 0.024

5.457 ± 0.045

4.895 ± 0.034

6.31 ± 0.032

5.325 ± 0.026

6.795 ± 0.031

1.278 ± 0.017

3.013 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski