Actinomyces sp. S6-Spd3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095XXY9|A0A095XXY9_9ACTO Glycogen debranching protein OS=Actinomyces sp. S6-Spd3 OX=1284680 GN=HMPREF1627_09000 PE=3 SV=1
MM1 pKa = 7.57TYY3 pKa = 10.6VIAQPCVDD11 pKa = 3.66VKK13 pKa = 11.25DD14 pKa = 3.94RR15 pKa = 11.84ACVDD19 pKa = 3.71EE20 pKa = 5.5CPVDD24 pKa = 4.23CIYY27 pKa = 10.95EE28 pKa = 4.28GEE30 pKa = 4.17RR31 pKa = 11.84TLYY34 pKa = 9.79IHH36 pKa = 7.51PDD38 pKa = 3.07EE39 pKa = 5.5CVDD42 pKa = 4.38CGACEE47 pKa = 3.97PVCPTEE53 pKa = 4.64AIFYY57 pKa = 10.42EE58 pKa = 4.83DD59 pKa = 4.84DD60 pKa = 5.65LPGEE64 pKa = 4.06WSDD67 pKa = 3.89YY68 pKa = 10.55LQANADD74 pKa = 3.75FFVDD78 pKa = 4.05LGSPGGAQKK87 pKa = 9.57TGPQSFDD94 pKa = 3.57VPWISALPPQNEE106 pKa = 4.45DD107 pKa = 2.34WDD109 pKa = 4.09KK110 pKa = 11.79

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095WU91|A0A095WU91_9ACTO Membrane protein OS=Actinomyces sp. S6-Spd3 OX=1284680 GN=HMPREF1627_02535 PE=4 SV=1
MM1 pKa = 6.05TTKK4 pKa = 9.87RR5 pKa = 11.84TYY7 pKa = 10.06QPNNRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84SKK16 pKa = 8.58THH18 pKa = 5.46GFRR21 pKa = 11.84LRR23 pKa = 11.84MSTRR27 pKa = 11.84AGRR30 pKa = 11.84AILAARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.64GRR41 pKa = 11.84ARR43 pKa = 11.84LSAA46 pKa = 3.91

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1762

0

1762

577363

37

3046

327.7

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.229 ± 0.077

0.826 ± 0.02

5.749 ± 0.051

6.309 ± 0.061

3.329 ± 0.041

8.064 ± 0.046

2.118 ± 0.027

5.38 ± 0.043

3.012 ± 0.04

9.703 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.027

2.626 ± 0.035

4.96 ± 0.037

3.562 ± 0.04

6.46 ± 0.06

6.87 ± 0.056

5.77 ± 0.057

7.918 ± 0.053

1.494 ± 0.028

2.286 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski