Amino acid dipepetide frequency for Actinomyces sp. S6-Spd3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.841AlaAla: 12.841 ± 0.179
1.034AlaCys: 1.034 ± 0.05
6.055AlaAsp: 6.055 ± 0.113
6.218AlaGlu: 6.218 ± 0.122
3.493AlaPhe: 3.493 ± 0.089
8.942AlaGly: 8.942 ± 0.127
2.468AlaHis: 2.468 ± 0.071
5.728AlaIle: 5.728 ± 0.116
3.409AlaLys: 3.409 ± 0.082
12.54AlaLeu: 12.54 ± 0.192
2.783AlaMet: 2.783 ± 0.075
2.719AlaAsn: 2.719 ± 0.079
5.239AlaPro: 5.239 ± 0.143
5.281AlaGln: 5.281 ± 0.125
7.709AlaArg: 7.709 ± 0.16
7.941AlaSer: 7.941 ± 0.123
5.923AlaThr: 5.923 ± 0.123
7.903AlaVal: 7.903 ± 0.146
1.632AlaTrp: 1.632 ± 0.057
2.43AlaTyr: 2.43 ± 0.08
0.0AlaXaa: 0.0 ± 0.0
Cys
1.247CysAla: 1.247 ± 0.055
0.085CysCys: 0.085 ± 0.013
0.485CysAsp: 0.485 ± 0.028
0.565CysGlu: 0.565 ± 0.03
0.286CysPhe: 0.286 ± 0.02
0.882CysGly: 0.882 ± 0.046
0.165CysHis: 0.165 ± 0.017
0.35CysIle: 0.35 ± 0.027
0.128CysLys: 0.128 ± 0.016
0.693CysLeu: 0.693 ± 0.037
0.173CysMet: 0.173 ± 0.017
0.166CysAsn: 0.166 ± 0.017
0.379CysPro: 0.379 ± 0.028
0.236CysGln: 0.236 ± 0.021
0.436CysArg: 0.436 ± 0.029
0.554CysSer: 0.554 ± 0.027
0.478CysThr: 0.478 ± 0.029
0.703CysVal: 0.703 ± 0.032
0.087CysTrp: 0.087 ± 0.013
0.161CysTyr: 0.161 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
6.389AspAla: 6.389 ± 0.112
0.456AspCys: 0.456 ± 0.028
3.305AspAsp: 3.305 ± 0.082
4.372AspGlu: 4.372 ± 0.092
2.113AspPhe: 2.113 ± 0.061
4.644AspGly: 4.644 ± 0.111
1.252AspHis: 1.252 ± 0.048
3.029AspIle: 3.029 ± 0.076
1.367AspLys: 1.367 ± 0.055
5.967AspLeu: 5.967 ± 0.111
1.154AspMet: 1.154 ± 0.046
1.294AspAsn: 1.294 ± 0.052
3.639AspPro: 3.639 ± 0.082
2.091AspGln: 2.091 ± 0.066
3.284AspArg: 3.284 ± 0.082
3.608AspSer: 3.608 ± 0.092
2.795AspThr: 2.795 ± 0.084
4.403AspVal: 4.403 ± 0.094
0.783AspTrp: 0.783 ± 0.04
1.55AspTyr: 1.55 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
7.534GluAla: 7.534 ± 0.158
0.502GluCys: 0.502 ± 0.029
3.89GluAsp: 3.89 ± 0.102
5.127GluGlu: 5.127 ± 0.133
1.858GluPhe: 1.858 ± 0.057
4.725GluGly: 4.725 ± 0.098
1.457GluHis: 1.457 ± 0.057
3.559GluIle: 3.559 ± 0.087
2.241GluLys: 2.241 ± 0.065
6.45GluLeu: 6.45 ± 0.106
1.431GluMet: 1.431 ± 0.052
1.971GluAsn: 1.971 ± 0.057
2.092GluPro: 2.092 ± 0.069
2.326GluGln: 2.326 ± 0.07
4.484GluArg: 4.484 ± 0.099
3.39GluSer: 3.39 ± 0.083
3.003GluThr: 3.003 ± 0.073
4.881GluVal: 4.881 ± 0.101
0.83GluTrp: 0.83 ± 0.034
1.339GluTyr: 1.339 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.729PheAla: 3.729 ± 0.085
0.258PheCys: 0.258 ± 0.022
2.233PheAsp: 2.233 ± 0.069
2.007PheGlu: 2.007 ± 0.055
1.335PhePhe: 1.335 ± 0.049
2.92PheGly: 2.92 ± 0.076
0.67PheHis: 0.67 ± 0.032
1.715PheIle: 1.715 ± 0.058
0.878PheLys: 0.878 ± 0.04
3.074PheLeu: 3.074 ± 0.094
0.714PheMet: 0.714 ± 0.035
0.949PheAsn: 0.949 ± 0.039
1.711PhePro: 1.711 ± 0.056
0.958PheGln: 0.958 ± 0.045
1.569PheArg: 1.569 ± 0.06
2.51PheSer: 2.51 ± 0.065
2.203PheThr: 2.203 ± 0.06
2.716PheVal: 2.716 ± 0.073
0.395PheTrp: 0.395 ± 0.03
0.743PheTyr: 0.743 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
8.435GlyAla: 8.435 ± 0.132
0.689GlyCys: 0.689 ± 0.04
4.256GlyAsp: 4.256 ± 0.089
4.997GlyGlu: 4.997 ± 0.1
2.889GlyPhe: 2.889 ± 0.064
6.266GlyGly: 6.266 ± 0.132
1.827GlyHis: 1.827 ± 0.064
5.042GlyIle: 5.042 ± 0.109
3.057GlyLys: 3.057 ± 0.084
7.465GlyLeu: 7.465 ± 0.138
2.066GlyMet: 2.066 ± 0.068
2.16GlyAsn: 2.16 ± 0.058
2.941GlyPro: 2.941 ± 0.065
3.076GlyGln: 3.076 ± 0.095
5.087GlyArg: 5.087 ± 0.098
5.305GlySer: 5.305 ± 0.1
4.893GlyThr: 4.893 ± 0.101
6.647GlyVal: 6.647 ± 0.109
1.344GlyTrp: 1.344 ± 0.048
2.196GlyTyr: 2.196 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.239HisAla: 2.239 ± 0.064
0.23HisCys: 0.23 ± 0.021
1.193HisAsp: 1.193 ± 0.046
1.266HisGlu: 1.266 ± 0.049
0.772HisPhe: 0.772 ± 0.038
1.658HisGly: 1.658 ± 0.057
0.468HisHis: 0.468 ± 0.028
1.017HisIle: 1.017 ± 0.036
0.533HisLys: 0.533 ± 0.029
2.03HisLeu: 2.03 ± 0.062
0.537HisMet: 0.537 ± 0.031
0.528HisAsn: 0.528 ± 0.027
1.401HisPro: 1.401 ± 0.056
0.65HisGln: 0.65 ± 0.032
1.38HisArg: 1.38 ± 0.046
1.483HisSer: 1.483 ± 0.059
1.244HisThr: 1.244 ± 0.041
1.671HisVal: 1.671 ± 0.06
0.327HisTrp: 0.327 ± 0.025
0.553HisTyr: 0.553 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.8IleAla: 6.8 ± 0.122
0.497IleCys: 0.497 ± 0.028
3.772IleAsp: 3.772 ± 0.087
3.467IleGlu: 3.467 ± 0.086
1.772IlePhe: 1.772 ± 0.062
4.521IleGly: 4.521 ± 0.108
1.197IleHis: 1.197 ± 0.045
2.686IleIle: 2.686 ± 0.078
1.244IleLys: 1.244 ± 0.06
4.623IleLeu: 4.623 ± 0.101
1.069IleMet: 1.069 ± 0.049
1.389IleAsn: 1.389 ± 0.051
3.163IlePro: 3.163 ± 0.074
1.405IleGln: 1.405 ± 0.053
3.114IleArg: 3.114 ± 0.081
3.537IleSer: 3.537 ± 0.098
3.246IleThr: 3.246 ± 0.071
4.676IleVal: 4.676 ± 0.103
0.551IleTrp: 0.551 ± 0.028
1.077IleTyr: 1.077 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
3.228LysAla: 3.228 ± 0.084
0.163LysCys: 0.163 ± 0.018
1.876LysAsp: 1.876 ± 0.068
1.985LysGlu: 1.985 ± 0.067
0.733LysPhe: 0.733 ± 0.036
2.245LysGly: 2.245 ± 0.064
0.516LysHis: 0.516 ± 0.028
1.69LysIle: 1.69 ± 0.061
1.46LysLys: 1.46 ± 0.066
2.558LysLeu: 2.558 ± 0.077
0.788LysMet: 0.788 ± 0.041
1.015LysAsn: 1.015 ± 0.05
1.413LysPro: 1.413 ± 0.048
1.062LysGln: 1.062 ± 0.05
2.165LysArg: 2.165 ± 0.072
1.682LysSer: 1.682 ± 0.067
1.86LysThr: 1.86 ± 0.071
2.499LysVal: 2.499 ± 0.078
0.445LysTrp: 0.445 ± 0.028
0.736LysTyr: 0.736 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
11.508LeuAla: 11.508 ± 0.166
0.757LeuCys: 0.757 ± 0.043
5.778LeuAsp: 5.778 ± 0.104
5.851LeuGlu: 5.851 ± 0.119
3.002LeuPhe: 3.002 ± 0.082
8.308LeuGly: 8.308 ± 0.131
1.935LeuHis: 1.935 ± 0.06
5.286LeuIle: 5.286 ± 0.121
2.83LeuLys: 2.83 ± 0.086
8.929LeuLeu: 8.929 ± 0.152
2.146LeuMet: 2.146 ± 0.063
2.459LeuAsn: 2.459 ± 0.069
5.274LeuPro: 5.274 ± 0.107
2.491LeuGln: 2.491 ± 0.068
6.594LeuArg: 6.594 ± 0.134
7.207LeuSer: 7.207 ± 0.126
5.804LeuThr: 5.804 ± 0.106
7.843LeuVal: 7.843 ± 0.14
1.251LeuTrp: 1.251 ± 0.052
1.779LeuTyr: 1.779 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.072
0.192MetCys: 0.192 ± 0.017
1.225MetAsp: 1.225 ± 0.051
1.162MetGlu: 1.162 ± 0.046
0.646MetPhe: 0.646 ± 0.032
1.959MetGly: 1.959 ± 0.07
0.443MetHis: 0.443 ± 0.027
1.221MetIle: 1.221 ± 0.048
0.783MetLys: 0.783 ± 0.041
2.101MetLeu: 2.101 ± 0.068
0.594MetMet: 0.594 ± 0.039
0.84MetAsn: 0.84 ± 0.04
1.173MetPro: 1.173 ± 0.047
0.587MetGln: 0.587 ± 0.032
1.781MetArg: 1.781 ± 0.062
2.025MetSer: 2.025 ± 0.065
1.846MetThr: 1.846 ± 0.058
1.689MetVal: 1.689 ± 0.052
0.301MetTrp: 0.301 ± 0.027
0.417MetTyr: 0.417 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.003AsnAla: 3.003 ± 0.088
0.222AsnCys: 0.222 ± 0.019
1.427AsnAsp: 1.427 ± 0.054
1.488AsnGlu: 1.488 ± 0.057
0.876AsnPhe: 0.876 ± 0.039
2.298AsnGly: 2.298 ± 0.091
0.507AsnHis: 0.507 ± 0.031
1.37AsnIle: 1.37 ± 0.061
0.703AsnLys: 0.703 ± 0.038
2.51AsnLeu: 2.51 ± 0.065
0.62AsnMet: 0.62 ± 0.034
0.639AsnAsn: 0.639 ± 0.036
1.966AsnPro: 1.966 ± 0.056
0.908AsnGln: 0.908 ± 0.043
1.49AsnArg: 1.49 ± 0.052
1.699AsnSer: 1.699 ± 0.065
1.41AsnThr: 1.41 ± 0.057
1.971AsnVal: 1.971 ± 0.062
0.424AsnTrp: 0.424 ± 0.028
0.724AsnTyr: 0.724 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
5.411ProAla: 5.411 ± 0.142
0.3ProCys: 0.3 ± 0.02
2.718ProAsp: 2.718 ± 0.071
3.916ProGlu: 3.916 ± 0.098
1.67ProPhe: 1.67 ± 0.054
3.997ProGly: 3.997 ± 0.09
1.053ProHis: 1.053 ± 0.044
2.29ProIle: 2.29 ± 0.069
1.535ProLys: 1.535 ± 0.052
4.749ProLeu: 4.749 ± 0.093
0.982ProMet: 0.982 ± 0.043
1.358ProAsn: 1.358 ± 0.051
1.593ProPro: 1.593 ± 0.065
2.304ProGln: 2.304 ± 0.079
2.906ProArg: 2.906 ± 0.076
3.758ProSer: 3.758 ± 0.102
3.152ProThr: 3.152 ± 0.096
3.944ProVal: 3.944 ± 0.084
0.778ProTrp: 0.778 ± 0.037
1.19ProTyr: 1.19 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
4.337GlnAla: 4.337 ± 0.102
0.268GlnCys: 0.268 ± 0.021
1.656GlnAsp: 1.656 ± 0.053
2.078GlnGlu: 2.078 ± 0.06
1.001GlnPhe: 1.001 ± 0.037
2.889GlnGly: 2.889 ± 0.083
0.688GlnHis: 0.688 ± 0.033
2.118GlnIle: 2.118 ± 0.059
1.051GlnLys: 1.051 ± 0.045
3.473GlnLeu: 3.473 ± 0.085
0.996GlnMet: 0.996 ± 0.036
0.892GlnAsn: 0.892 ± 0.044
1.581GlnPro: 1.581 ± 0.081
1.372GlnGln: 1.372 ± 0.094
2.548GlnArg: 2.548 ± 0.073
2.11GlnSer: 2.11 ± 0.066
1.864GlnThr: 1.864 ± 0.056
3.029GlnVal: 3.029 ± 0.076
0.842GlnTrp: 0.842 ± 0.048
0.828GlnTyr: 0.828 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
7.221ArgAla: 7.221 ± 0.137
0.445ArgCys: 0.445 ± 0.032
3.353ArgAsp: 3.353 ± 0.092
4.761ArgGlu: 4.761 ± 0.113
2.193ArgPhe: 2.193 ± 0.066
4.798ArgGly: 4.798 ± 0.104
1.386ArgHis: 1.386 ± 0.044
3.53ArgIle: 3.53 ± 0.079
2.039ArgLys: 2.039 ± 0.068
6.157ArgLeu: 6.157 ± 0.122
1.666ArgMet: 1.666 ± 0.057
1.486ArgAsn: 1.486 ± 0.051
2.96ArgPro: 2.96 ± 0.085
2.378ArgGln: 2.378 ± 0.071
4.941ArgArg: 4.941 ± 0.124
4.382ArgSer: 4.382 ± 0.102
3.299ArgThr: 3.299 ± 0.079
5.007ArgVal: 5.007 ± 0.1
1.043ArgTrp: 1.043 ± 0.043
1.55ArgTyr: 1.55 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
7.881SerAla: 7.881 ± 0.133
0.544SerCys: 0.544 ± 0.032
3.812SerAsp: 3.812 ± 0.098
3.954SerGlu: 3.954 ± 0.083
2.551SerPhe: 2.551 ± 0.063
6.0SerGly: 6.0 ± 0.107
1.379SerHis: 1.379 ± 0.051
3.372SerIle: 3.372 ± 0.081
1.867SerLys: 1.867 ± 0.057
6.452SerLeu: 6.452 ± 0.124
1.671SerMet: 1.671 ± 0.057
1.583SerAsn: 1.583 ± 0.057
3.485SerPro: 3.485 ± 0.079
2.744SerGln: 2.744 ± 0.073
4.091SerArg: 4.091 ± 0.091
5.309SerSer: 5.309 ± 0.129
4.195SerThr: 4.195 ± 0.097
5.07SerVal: 5.07 ± 0.106
1.088SerTrp: 1.088 ± 0.043
1.656SerTyr: 1.656 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.613ThrAla: 5.613 ± 0.106
0.475ThrCys: 0.475 ± 0.03
3.104ThrAsp: 3.104 ± 0.081
2.792ThrGlu: 2.792 ± 0.07
1.964ThrPhe: 1.964 ± 0.062
4.597ThrGly: 4.597 ± 0.096
1.472ThrHis: 1.472 ± 0.057
3.204ThrIle: 3.204 ± 0.084
1.637ThrLys: 1.637 ± 0.058
5.771ThrLeu: 5.771 ± 0.112
1.249ThrMet: 1.249 ± 0.042
1.561ThrAsn: 1.561 ± 0.071
3.855ThrPro: 3.855 ± 0.123
2.274ThrGln: 2.274 ± 0.06
3.58ThrArg: 3.58 ± 0.079
3.935ThrSer: 3.935 ± 0.086
3.559ThrThr: 3.559 ± 0.121
4.599ThrVal: 4.599 ± 0.115
0.979ThrTrp: 0.979 ± 0.042
1.481ThrTyr: 1.481 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
8.501ValAla: 8.501 ± 0.139
0.824ValCys: 0.824 ± 0.045
5.07ValAsp: 5.07 ± 0.104
4.872ValGlu: 4.872 ± 0.104
2.749ValPhe: 2.749 ± 0.077
5.922ValGly: 5.922 ± 0.114
1.557ValHis: 1.557 ± 0.052
4.713ValIle: 4.713 ± 0.1
2.193ValLys: 2.193 ± 0.074
7.759ValLeu: 7.759 ± 0.16
1.746ValMet: 1.746 ± 0.067
2.106ValAsn: 2.106 ± 0.068
4.006ValPro: 4.006 ± 0.093
2.186ValGln: 2.186 ± 0.067
5.011ValArg: 5.011 ± 0.11
5.674ValSer: 5.674 ± 0.12
4.704ValThr: 4.704 ± 0.124
6.912ValVal: 6.912 ± 0.133
1.152ValTrp: 1.152 ± 0.047
1.522ValTyr: 1.522 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
1.567TrpAla: 1.567 ± 0.051
0.123TrpCys: 0.123 ± 0.016
0.986TrpAsp: 0.986 ± 0.042
0.849TrpGlu: 0.849 ± 0.044
0.535TrpPhe: 0.535 ± 0.029
1.124TrpGly: 1.124 ± 0.049
0.289TrpHis: 0.289 ± 0.022
0.878TrpIle: 0.878 ± 0.047
0.542TrpLys: 0.542 ± 0.033
1.446TrpLeu: 1.446 ± 0.053
0.459TrpMet: 0.459 ± 0.028
0.551TrpAsn: 0.551 ± 0.033
0.504TrpPro: 0.504 ± 0.027
0.514TrpGln: 0.514 ± 0.027
0.904TrpArg: 0.904 ± 0.04
0.944TrpSer: 0.944 ± 0.045
0.85TrpThr: 0.85 ± 0.039
1.207TrpVal: 1.207 ± 0.048
0.319TrpTrp: 0.319 ± 0.025
0.345TrpTyr: 0.345 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.442TyrAla: 2.442 ± 0.067
0.194TyrCys: 0.194 ± 0.019
1.301TyrAsp: 1.301 ± 0.054
1.363TyrGlu: 1.363 ± 0.048
0.849TyrPhe: 0.849 ± 0.045
1.945TyrGly: 1.945 ± 0.057
0.452TyrHis: 0.452 ± 0.028
1.006TyrIle: 1.006 ± 0.042
0.624TyrLys: 0.624 ± 0.041
2.283TyrLeu: 2.283 ± 0.067
0.504TyrMet: 0.504 ± 0.029
0.589TyrAsn: 0.589 ± 0.033
1.226TyrPro: 1.226 ± 0.046
0.781TyrGln: 0.781 ± 0.043
1.521TyrArg: 1.521 ± 0.058
1.651TyrSer: 1.651 ± 0.062
1.37TyrThr: 1.37 ± 0.055
1.808TyrVal: 1.808 ± 0.061
0.369TyrTrp: 0.369 ± 0.026
0.58TyrTyr: 0.58 ± 0.039
0.003TyrXaa: 0.003 ± 0.002
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.003XaaTyr: 0.003 ± 0.002
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1762 proteins (577363 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski