Clostridium sp. FS41

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0CK99|A0A0F0CK99_9CLOT Uncharacterized protein OS=Clostridium sp. FS41 OX=1609975 GN=CLFS41_12430 PE=4 SV=1
MM1 pKa = 7.25QMRR4 pKa = 11.84GCGSQGEE11 pKa = 4.46WVDD14 pKa = 3.61PYY16 pKa = 11.39KK17 pKa = 11.43VNTCFLDD24 pKa = 2.94TGYY27 pKa = 11.14FDD29 pKa = 3.34TCTFEE34 pKa = 3.83KK35 pKa = 10.29TPYY38 pKa = 10.41SNARR42 pKa = 11.84YY43 pKa = 8.73PYY45 pKa = 9.25QTVCDD50 pKa = 3.76NGLGLGYY57 pKa = 10.63YY58 pKa = 7.75EE59 pKa = 4.43VRR61 pKa = 11.84CPEE64 pKa = 3.81NSEE67 pKa = 4.27FDD69 pKa = 3.99PSTLRR74 pKa = 11.84CKK76 pKa = 10.33SVCEE80 pKa = 4.04YY81 pKa = 11.13GKK83 pKa = 10.26NPDD86 pKa = 3.94GTCMDD91 pKa = 3.88ACQFKK96 pKa = 10.92KK97 pKa = 10.76SIDD100 pKa = 4.02EE101 pKa = 4.15IKK103 pKa = 10.48SLQWLAYY110 pKa = 9.88VYY112 pKa = 10.64GEE114 pKa = 4.11QVTGSCYY121 pKa = 10.68GDD123 pKa = 3.56YY124 pKa = 11.09GATRR128 pKa = 11.84CEE130 pKa = 4.25LEE132 pKa = 4.18RR133 pKa = 11.84TPSDD137 pKa = 3.21STLCTGVDD145 pKa = 3.31SGQWTQNTICHH156 pKa = 6.32GNFQFTGNQCEE167 pKa = 4.28GGTLFWGKK175 pKa = 10.11DD176 pKa = 3.62GPDD179 pKa = 3.31TPIIPDD185 pKa = 4.37DD186 pKa = 5.14PIHH189 pKa = 7.61DD190 pKa = 4.88PDD192 pKa = 5.88DD193 pKa = 3.89PTGDD197 pKa = 3.64IEE199 pKa = 5.54DD200 pKa = 4.5PSILPDD206 pKa = 3.61GSTNTVNPPDD216 pKa = 4.02TDD218 pKa = 3.65SEE220 pKa = 4.63PDD222 pKa = 3.48VEE224 pKa = 5.55EE225 pKa = 5.22PDD227 pKa = 3.63TDD229 pKa = 3.49EE230 pKa = 4.45STDD233 pKa = 3.64TAVLKK238 pKa = 10.91AITGMNKK245 pKa = 10.0DD246 pKa = 3.38VNKK249 pKa = 10.54ALNDD253 pKa = 3.47MNIDD257 pKa = 3.4INQANADD264 pKa = 3.75VQNQIIALNASMVTNTQAIQKK285 pKa = 7.84QQINDD290 pKa = 3.14NKK292 pKa = 10.37IYY294 pKa = 10.49EE295 pKa = 4.16NTKK298 pKa = 10.95ALIQQANADD307 pKa = 3.49ITTAMNKK314 pKa = 7.52NTNAVNGVGDD324 pKa = 4.45DD325 pKa = 3.72VEE327 pKa = 5.55KK328 pKa = 10.74IAGAMDD334 pKa = 5.45GIAEE338 pKa = 4.26DD339 pKa = 3.65VSGISDD345 pKa = 3.9TLDD348 pKa = 4.1GIANTDD354 pKa = 3.08TSGAGTGGTCIEE366 pKa = 4.43SQSCTGFYY374 pKa = 10.69EE375 pKa = 4.04SGYY378 pKa = 9.68PDD380 pKa = 3.62GLGGLVSGQLDD391 pKa = 3.73DD392 pKa = 5.58LKK394 pKa = 11.35HH395 pKa = 4.84NTIDD399 pKa = 3.42NFVNSFGDD407 pKa = 3.73LDD409 pKa = 4.02LSSAKK414 pKa = 10.1RR415 pKa = 11.84PSFVLPVPFFGDD427 pKa = 3.76FSFEE431 pKa = 4.02EE432 pKa = 4.39QISFDD437 pKa = 3.32WVFGFIRR444 pKa = 11.84AVLIMTSVFAARR456 pKa = 11.84RR457 pKa = 11.84IIFGGG462 pKa = 3.39

Molecular weight: 50.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0CI08|A0A0F0CI08_9CLOT Orotate phosphoribosyltransferase OS=Clostridium sp. FS41 OX=1609975 GN=pyrE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.82TVGGRR28 pKa = 11.84KK29 pKa = 9.22VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5722

0

5722

1853055

29

4083

323.8

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.891 ± 0.033

1.585 ± 0.013

5.587 ± 0.028

6.972 ± 0.035

4.023 ± 0.022

7.957 ± 0.031

1.792 ± 0.013

6.934 ± 0.029

5.646 ± 0.027

9.095 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.473 ± 0.016

3.868 ± 0.018

3.64 ± 0.022

3.396 ± 0.019

4.953 ± 0.029

5.908 ± 0.023

5.271 ± 0.023

6.99 ± 0.027

1.026 ± 0.012

3.991 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski