Amino acid dipepetide frequency for Clostridium sp. FS41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.751AlaAla: 8.751 ± 0.093
1.258AlaCys: 1.258 ± 0.026
4.469AlaAsp: 4.469 ± 0.057
4.807AlaGlu: 4.807 ± 0.055
3.154AlaPhe: 3.154 ± 0.045
7.527AlaGly: 7.527 ± 0.079
1.142AlaHis: 1.142 ± 0.029
4.76AlaIle: 4.76 ± 0.058
3.901AlaLys: 3.901 ± 0.051
7.359AlaLeu: 7.359 ± 0.073
2.837AlaMet: 2.837 ± 0.042
2.356AlaAsn: 2.356 ± 0.035
2.364AlaPro: 2.364 ± 0.06
2.591AlaGln: 2.591 ± 0.04
3.609AlaArg: 3.609 ± 0.043
4.353AlaSer: 4.353 ± 0.059
3.044AlaThr: 3.044 ± 0.047
7.075AlaVal: 7.075 ± 0.061
0.791AlaTrp: 0.791 ± 0.021
2.762AlaTyr: 2.762 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.132CysAla: 1.132 ± 0.026
0.343CysCys: 0.343 ± 0.016
0.767CysAsp: 0.767 ± 0.024
0.818CysGlu: 0.818 ± 0.021
0.703CysPhe: 0.703 ± 0.02
1.61CysGly: 1.61 ± 0.03
0.331CysHis: 0.331 ± 0.011
1.122CysIle: 1.122 ± 0.025
0.658CysLys: 0.658 ± 0.018
1.447CysLeu: 1.447 ± 0.03
0.523CysMet: 0.523 ± 0.016
0.59CysAsn: 0.59 ± 0.02
0.723CysPro: 0.723 ± 0.023
0.472CysGln: 0.472 ± 0.014
0.965CysArg: 0.965 ± 0.025
1.043CysSer: 1.043 ± 0.025
0.81CysThr: 0.81 ± 0.023
1.059CysVal: 1.059 ± 0.024
0.145CysTrp: 0.145 ± 0.009
0.59CysTyr: 0.59 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.84AspAla: 3.84 ± 0.056
0.866AspCys: 0.866 ± 0.024
2.675AspAsp: 2.675 ± 0.048
4.066AspGlu: 4.066 ± 0.055
2.403AspPhe: 2.403 ± 0.035
4.744AspGly: 4.744 ± 0.077
1.169AspHis: 1.169 ± 0.027
4.197AspIle: 4.197 ± 0.054
2.965AspLys: 2.965 ± 0.041
4.679AspLeu: 4.679 ± 0.049
2.167AspMet: 2.167 ± 0.039
2.03AspAsn: 2.03 ± 0.034
2.033AspPro: 2.033 ± 0.038
1.943AspGln: 1.943 ± 0.038
3.071AspArg: 3.071 ± 0.038
3.165AspSer: 3.165 ± 0.046
2.996AspThr: 2.996 ± 0.04
3.678AspVal: 3.678 ± 0.049
0.649AspTrp: 0.649 ± 0.019
2.533AspTyr: 2.533 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
5.56GluAla: 5.56 ± 0.068
0.84GluCys: 0.84 ± 0.022
4.276GluAsp: 4.276 ± 0.053
6.285GluGlu: 6.285 ± 0.083
2.411GluPhe: 2.411 ± 0.039
4.753GluGly: 4.753 ± 0.062
1.466GluHis: 1.466 ± 0.029
4.737GluIle: 4.737 ± 0.055
4.831GluLys: 4.831 ± 0.058
6.214GluLeu: 6.214 ± 0.07
2.406GluMet: 2.406 ± 0.033
3.261GluAsn: 3.261 ± 0.044
2.006GluPro: 2.006 ± 0.035
2.975GluGln: 2.975 ± 0.048
3.469GluArg: 3.469 ± 0.053
3.285GluSer: 3.285 ± 0.046
3.522GluThr: 3.522 ± 0.05
3.938GluVal: 3.938 ± 0.057
0.635GluTrp: 0.635 ± 0.019
2.852GluTyr: 2.852 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
2.972PheAla: 2.972 ± 0.044
0.752PheCys: 0.752 ± 0.021
2.28PheAsp: 2.28 ± 0.033
2.344PheGlu: 2.344 ± 0.033
1.734PhePhe: 1.734 ± 0.039
3.153PheGly: 3.153 ± 0.049
0.805PheHis: 0.805 ± 0.021
2.753PheIle: 2.753 ± 0.048
1.99PheLys: 1.99 ± 0.034
3.963PheLeu: 3.963 ± 0.057
1.357PheMet: 1.357 ± 0.03
1.495PheAsn: 1.495 ± 0.038
1.477PhePro: 1.477 ± 0.028
1.278PheGln: 1.278 ± 0.027
1.779PheArg: 1.779 ± 0.035
2.821PheSer: 2.821 ± 0.037
2.375PheThr: 2.375 ± 0.04
2.733PheVal: 2.733 ± 0.04
0.439PheTrp: 0.439 ± 0.017
1.733PheTyr: 1.733 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
5.902GlyAla: 5.902 ± 0.08
1.515GlyCys: 1.515 ± 0.032
3.927GlyAsp: 3.927 ± 0.055
4.937GlyGlu: 4.937 ± 0.056
3.402GlyPhe: 3.402 ± 0.042
5.928GlyGly: 5.928 ± 0.08
1.386GlyHis: 1.386 ± 0.028
6.709GlyIle: 6.709 ± 0.07
4.882GlyLys: 4.882 ± 0.059
6.87GlyLeu: 6.87 ± 0.059
2.972GlyMet: 2.972 ± 0.042
3.172GlyAsn: 3.172 ± 0.046
1.927GlyPro: 1.927 ± 0.058
2.653GlyGln: 2.653 ± 0.045
4.142GlyArg: 4.142 ± 0.051
4.794GlySer: 4.794 ± 0.064
4.693GlyThr: 4.693 ± 0.054
5.385GlyVal: 5.385 ± 0.054
0.93GlyTrp: 0.93 ± 0.031
3.449GlyTyr: 3.449 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
1.113HisAla: 1.113 ± 0.025
0.319HisCys: 0.319 ± 0.012
0.868HisAsp: 0.868 ± 0.022
1.049HisGlu: 1.049 ± 0.031
0.858HisPhe: 0.858 ± 0.024
1.489HisGly: 1.489 ± 0.03
0.409HisHis: 0.409 ± 0.017
1.362HisIle: 1.362 ± 0.028
0.871HisLys: 0.871 ± 0.024
1.604HisLeu: 1.604 ± 0.031
0.703HisMet: 0.703 ± 0.02
0.679HisAsn: 0.679 ± 0.022
0.92HisPro: 0.92 ± 0.022
0.557HisGln: 0.557 ± 0.018
0.889HisArg: 0.889 ± 0.023
0.992HisSer: 0.992 ± 0.022
0.966HisThr: 0.966 ± 0.023
1.278HisVal: 1.278 ± 0.027
0.2HisTrp: 0.2 ± 0.011
0.795HisTyr: 0.795 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.365IleAla: 5.365 ± 0.07
1.304IleCys: 1.304 ± 0.025
3.514IleAsp: 3.514 ± 0.05
3.927IleGlu: 3.927 ± 0.045
2.65IlePhe: 2.65 ± 0.044
5.176IleGly: 5.176 ± 0.06
1.403IleHis: 1.403 ± 0.027
4.599IleIle: 4.599 ± 0.063
3.488IleLys: 3.488 ± 0.049
6.897IleLeu: 6.897 ± 0.074
2.118IleMet: 2.118 ± 0.039
2.625IleAsn: 2.625 ± 0.043
3.366IlePro: 3.366 ± 0.044
2.4IleGln: 2.4 ± 0.04
4.028IleArg: 4.028 ± 0.054
4.724IleSer: 4.724 ± 0.054
4.112IleThr: 4.112 ± 0.053
4.419IleVal: 4.419 ± 0.06
0.677IleTrp: 0.677 ± 0.019
2.549IleTyr: 2.549 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
4.725LysAla: 4.725 ± 0.055
0.587LysCys: 0.587 ± 0.019
3.5LysAsp: 3.5 ± 0.047
5.138LysGlu: 5.138 ± 0.052
1.496LysPhe: 1.496 ± 0.032
4.221LysGly: 4.221 ± 0.047
0.91LysHis: 0.91 ± 0.023
3.573LysIle: 3.573 ± 0.046
4.36LysLys: 4.36 ± 0.047
4.487LysLeu: 4.487 ± 0.053
1.876LysMet: 1.876 ± 0.029
2.582LysAsn: 2.582 ± 0.038
1.853LysPro: 1.853 ± 0.032
2.138LysGln: 2.138 ± 0.034
2.931LysArg: 2.931 ± 0.042
2.762LysSer: 2.762 ± 0.041
3.093LysThr: 3.093 ± 0.043
3.531LysVal: 3.531 ± 0.052
0.568LysTrp: 0.568 ± 0.018
2.133LysTyr: 2.133 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
7.341LeuAla: 7.341 ± 0.065
1.613LeuCys: 1.613 ± 0.03
5.314LeuAsp: 5.314 ± 0.062
6.339LeuGlu: 6.339 ± 0.073
3.944LeuPhe: 3.944 ± 0.051
6.639LeuGly: 6.639 ± 0.072
1.472LeuHis: 1.472 ± 0.031
5.806LeuIle: 5.806 ± 0.064
5.702LeuLys: 5.702 ± 0.065
8.598LeuLeu: 8.598 ± 0.101
2.966LeuMet: 2.966 ± 0.045
3.534LeuAsn: 3.534 ± 0.048
3.777LeuPro: 3.777 ± 0.048
2.562LeuGln: 2.562 ± 0.04
3.881LeuArg: 3.881 ± 0.041
6.248LeuSer: 6.248 ± 0.07
5.085LeuThr: 5.085 ± 0.058
5.883LeuVal: 5.883 ± 0.057
0.841LeuTrp: 0.841 ± 0.023
3.403LeuTyr: 3.403 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
3.097MetAla: 3.097 ± 0.046
0.406MetCys: 0.406 ± 0.014
2.358MetAsp: 2.358 ± 0.032
2.995MetGlu: 2.995 ± 0.036
1.221MetPhe: 1.221 ± 0.027
2.766MetGly: 2.766 ± 0.038
0.426MetHis: 0.426 ± 0.015
2.354MetIle: 2.354 ± 0.041
2.415MetLys: 2.415 ± 0.036
2.878MetLeu: 2.878 ± 0.036
1.149MetMet: 1.149 ± 0.026
1.527MetAsn: 1.527 ± 0.029
1.294MetPro: 1.294 ± 0.028
0.986MetGln: 0.986 ± 0.024
1.439MetArg: 1.439 ± 0.032
1.893MetSer: 1.893 ± 0.033
1.904MetThr: 1.904 ± 0.032
2.426MetVal: 2.426 ± 0.043
0.26MetTrp: 0.26 ± 0.012
0.934MetTyr: 0.934 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.841AsnAla: 2.841 ± 0.041
0.595AsnCys: 0.595 ± 0.017
1.783AsnAsp: 1.783 ± 0.033
2.243AsnGlu: 2.243 ± 0.04
1.387AsnPhe: 1.387 ± 0.028
3.341AsnGly: 3.341 ± 0.054
0.79AsnHis: 0.79 ± 0.021
2.837AsnIle: 2.837 ± 0.04
1.92AsnLys: 1.92 ± 0.031
3.492AsnLeu: 3.492 ± 0.051
1.416AsnMet: 1.416 ± 0.027
1.438AsnAsn: 1.438 ± 0.028
1.96AsnPro: 1.96 ± 0.036
1.501AsnGln: 1.501 ± 0.028
2.209AsnArg: 2.209 ± 0.039
2.169AsnSer: 2.169 ± 0.047
2.115AsnThr: 2.115 ± 0.035
2.645AsnVal: 2.645 ± 0.034
0.427AsnTrp: 0.427 ± 0.015
1.572AsnTyr: 1.572 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
2.837ProAla: 2.837 ± 0.043
0.547ProCys: 0.547 ± 0.019
2.659ProAsp: 2.659 ± 0.044
3.187ProGlu: 3.187 ± 0.042
1.592ProPhe: 1.592 ± 0.031
3.173ProGly: 3.173 ± 0.042
0.693ProHis: 0.693 ± 0.021
2.058ProIle: 2.058 ± 0.035
1.617ProLys: 1.617 ± 0.035
3.123ProLeu: 3.123 ± 0.047
1.127ProMet: 1.127 ± 0.024
1.15ProAsn: 1.15 ± 0.023
0.969ProPro: 0.969 ± 0.025
1.253ProGln: 1.253 ± 0.029
1.294ProArg: 1.294 ± 0.026
2.128ProSer: 2.128 ± 0.035
1.509ProThr: 1.509 ± 0.033
3.555ProVal: 3.555 ± 0.087
0.387ProTrp: 0.387 ± 0.013
1.548ProTyr: 1.548 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.963GlnAla: 2.963 ± 0.035
0.414GlnCys: 0.414 ± 0.015
1.801GlnAsp: 1.801 ± 0.033
2.805GlnGlu: 2.805 ± 0.043
1.192GlnPhe: 1.192 ± 0.028
2.593GlnGly: 2.593 ± 0.054
0.467GlnHis: 0.467 ± 0.017
2.393GlnIle: 2.393 ± 0.037
2.117GlnLys: 2.117 ± 0.037
2.871GlnLeu: 2.871 ± 0.044
1.375GlnMet: 1.375 ± 0.028
1.373GlnAsn: 1.373 ± 0.031
1.076GlnPro: 1.076 ± 0.025
1.173GlnGln: 1.173 ± 0.025
1.481GlnArg: 1.481 ± 0.029
1.78GlnSer: 1.78 ± 0.029
1.73GlnThr: 1.73 ± 0.034
2.507GlnVal: 2.507 ± 0.035
0.435GlnTrp: 0.435 ± 0.017
1.413GlnTyr: 1.413 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
3.153ArgAla: 3.153 ± 0.04
0.713ArgCys: 0.713 ± 0.023
2.738ArgAsp: 2.738 ± 0.042
4.346ArgGlu: 4.346 ± 0.057
2.084ArgPhe: 2.084 ± 0.035
3.034ArgGly: 3.034 ± 0.041
0.911ArgHis: 0.911 ± 0.024
3.894ArgIle: 3.894 ± 0.045
3.067ArgLys: 3.067 ± 0.039
4.592ArgLeu: 4.592 ± 0.049
1.91ArgMet: 1.91 ± 0.028
2.044ArgAsn: 2.044 ± 0.036
1.631ArgPro: 1.631 ± 0.037
1.984ArgGln: 1.984 ± 0.034
2.744ArgArg: 2.744 ± 0.042
2.568ArgSer: 2.568 ± 0.037
2.584ArgThr: 2.584 ± 0.042
2.921ArgVal: 2.921 ± 0.041
0.484ArgTrp: 0.484 ± 0.019
2.121ArgTyr: 2.121 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
4.343SerAla: 4.343 ± 0.055
0.913SerCys: 0.913 ± 0.021
3.016SerAsp: 3.016 ± 0.05
3.323SerGlu: 3.323 ± 0.046
2.583SerPhe: 2.583 ± 0.043
5.732SerGly: 5.732 ± 0.069
1.174SerHis: 1.174 ± 0.027
4.058SerIle: 4.058 ± 0.048
2.651SerLys: 2.651 ± 0.041
5.659SerLeu: 5.659 ± 0.054
2.154SerMet: 2.154 ± 0.039
2.064SerAsn: 2.064 ± 0.034
2.07SerPro: 2.07 ± 0.037
2.108SerGln: 2.108 ± 0.037
3.252SerArg: 3.252 ± 0.042
3.817SerSer: 3.817 ± 0.057
2.819SerThr: 2.819 ± 0.039
4.336SerVal: 4.336 ± 0.048
0.64SerTrp: 0.64 ± 0.021
2.371SerTyr: 2.371 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
4.734ThrAla: 4.734 ± 0.058
0.722ThrCys: 0.722 ± 0.021
3.038ThrAsp: 3.038 ± 0.051
3.304ThrGlu: 3.304 ± 0.05
2.1ThrPhe: 2.1 ± 0.034
5.259ThrGly: 5.259 ± 0.068
0.812ThrHis: 0.812 ± 0.019
3.651ThrIle: 3.651 ± 0.047
2.369ThrLys: 2.369 ± 0.034
4.652ThrLeu: 4.652 ± 0.056
1.641ThrMet: 1.641 ± 0.031
1.782ThrAsn: 1.782 ± 0.03
2.272ThrPro: 2.272 ± 0.043
1.548ThrGln: 1.548 ± 0.027
2.261ThrArg: 2.261 ± 0.039
2.952ThrSer: 2.952 ± 0.038
2.642ThrThr: 2.642 ± 0.043
4.428ThrVal: 4.428 ± 0.051
0.531ThrTrp: 0.531 ± 0.019
2.018ThrTyr: 2.018 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
4.883ValAla: 4.883 ± 0.069
1.298ValCys: 1.298 ± 0.026
3.801ValAsp: 3.801 ± 0.052
4.415ValGlu: 4.415 ± 0.053
3.126ValPhe: 3.126 ± 0.042
4.508ValGly: 4.508 ± 0.06
1.107ValHis: 1.107 ± 0.029
5.243ValIle: 5.243 ± 0.059
3.948ValLys: 3.948 ± 0.052
6.9ValLeu: 6.9 ± 0.069
2.41ValMet: 2.41 ± 0.039
2.82ValAsn: 2.82 ± 0.045
2.822ValPro: 2.822 ± 0.036
2.0ValGln: 2.0 ± 0.031
3.409ValArg: 3.409 ± 0.042
4.725ValSer: 4.725 ± 0.053
4.137ValThr: 4.137 ± 0.057
4.901ValVal: 4.901 ± 0.062
0.71ValTrp: 0.71 ± 0.019
2.734ValTyr: 2.734 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.65TrpAla: 0.65 ± 0.019
0.166TrpCys: 0.166 ± 0.009
0.654TrpAsp: 0.654 ± 0.019
0.707TrpGlu: 0.707 ± 0.023
0.432TrpPhe: 0.432 ± 0.017
0.749TrpGly: 0.749 ± 0.022
0.187TrpHis: 0.187 ± 0.011
0.695TrpIle: 0.695 ± 0.021
0.76TrpLys: 0.76 ± 0.022
0.977TrpLeu: 0.977 ± 0.027
0.377TrpMet: 0.377 ± 0.014
0.583TrpAsn: 0.583 ± 0.018
0.275TrpPro: 0.275 ± 0.014
0.389TrpGln: 0.389 ± 0.015
0.442TrpArg: 0.442 ± 0.017
0.529TrpSer: 0.529 ± 0.017
0.467TrpThr: 0.467 ± 0.017
0.609TrpVal: 0.609 ± 0.02
0.132TrpTrp: 0.132 ± 0.008
0.486TrpTyr: 0.486 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.705TyrAla: 2.705 ± 0.042
0.678TyrCys: 0.678 ± 0.022
2.43TyrAsp: 2.43 ± 0.04
2.688TyrGlu: 2.688 ± 0.042
1.762TyrPhe: 1.762 ± 0.03
3.179TyrGly: 3.179 ± 0.041
0.863TyrHis: 0.863 ± 0.025
2.54TyrIle: 2.54 ± 0.035
1.951TyrLys: 1.951 ± 0.035
3.688TyrLeu: 3.688 ± 0.049
1.245TyrMet: 1.245 ± 0.026
1.577TyrAsn: 1.577 ± 0.032
1.59TyrPro: 1.59 ± 0.033
1.45TyrGln: 1.45 ± 0.031
2.231TyrArg: 2.231 ± 0.038
2.333TyrSer: 2.333 ± 0.035
2.113TyrThr: 2.113 ± 0.032
2.591TyrVal: 2.591 ± 0.037
0.382TyrTrp: 0.382 ± 0.014
1.914TyrTyr: 1.914 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5722 proteins (1853055 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski