Neoasaia chiangmaiensis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2999 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9KSH3|A0A1U9KSH3_9PROT Phosphatase OS=Neoasaia chiangmaiensis OX=320497 GN=A0U93_12965 PE=4 SV=1
MM1 pKa = 7.76 AIVTVVGASGTHH13 pKa = 4.41 VQVTVDD19 pKa = 3.48 GGQTQRR25 pKa = 11.84 LAQEE29 pKa = 4.37 YY30 pKa = 7.78 ATSIQNAFSGGTLDD44 pKa = 5.16 VVDD47 pKa = 5.37 LAPGSNQFASSAMSTVQGQITVGGSYY73 pKa = 9.2 TLNGSYY79 pKa = 10.95 TNVAVGALASNDD91 pKa = 3.76 SPVTPLNGQVTINAAGSTGSSLSILTGNQGNTDD124 pKa = 3.48 VTVGNQSGTFVAAAGTNAFHH144 pKa = 7.05 GGDD147 pKa = 3.84 STGDD151 pKa = 3.22 WQIATGAGTTNTLSLGTGANYY172 pKa = 9.72 VASEE176 pKa = 4.07 GHH178 pKa = 5.97 DD179 pKa = 3.95 TIDD182 pKa = 3.6 GAAGGTDD189 pKa = 3.31 TVTLIGGSSLVSLQQNAAVVDD210 pKa = 4.7 LSTGNNISVGDD221 pKa = 3.76 NSTVFGGSGSSANFTGDD238 pKa = 2.38 SSTIVGALGDD248 pKa = 4.23 TISAASSLQVIHH260 pKa = 6.92 GSNNDD265 pKa = 2.86 ISVSGNLSFLNGTGTTTIAAGNATIFGASGLNAITSTMSSPALFVGNEE313 pKa = 4.0 GPEE316 pKa = 4.36 TVNGASSTSALHH328 pKa = 6.71 AFAGSGNDD336 pKa = 3.38 VLIGGSASDD345 pKa = 3.63 TLVGGVGNATLTGGSGASNLFAITKK370 pKa = 9.73 DD371 pKa = 3.51 HH372 pKa = 7.14 AGGNYY377 pKa = 9.03 TITDD381 pKa = 4.46 FGSAAGNITALYY393 pKa = 10.62 NYY395 pKa = 9.81 GLQNNNGLQSVLNNATVAGGNTTIQLSDD423 pKa = 3.07 NSKK426 pKa = 8.04 ITFVGVTDD434 pKa = 5.32 LKK436 pKa = 11.38 ASDD439 pKa = 4.38 FNLSS443 pKa = 3.09
Molecular weight: 43.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.706
IPC_protein 3.745
Toseland 3.503
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.202
Thurlkill 3.579
EMBOSS 3.757
Sillero 3.872
Patrickios 0.744
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A1U9KP12|A0A1U9KP12_9PROT Transcriptional regulator OS=Neoasaia chiangmaiensis OX=320497 GN=A0U93_05840 PE=3 SV=1
MM1 pKa = 7.67 RR2 pKa = 11.84 FKK4 pKa = 11.26 NFFFAAILGLATVAASPNTHH24 pKa = 6.5 SDD26 pKa = 2.94 AKK28 pKa = 9.71 TQTTKK33 pKa = 10.64 VASLRR38 pKa = 11.84 QWPAPNATRR47 pKa = 11.84 VASLRR52 pKa = 11.84 QWPAPNAIRR61 pKa = 11.84 VASLRR66 pKa = 11.84 QWPAPNAIRR75 pKa = 11.84 VASLRR80 pKa = 11.84 QWPAPNATRR89 pKa = 11.84 VASLRR94 pKa = 11.84 QWPAPNATRR103 pKa = 11.84 VASLRR108 pKa = 11.84 QWPAPNAIRR117 pKa = 11.84 VASLRR122 pKa = 11.84 QWPAPNAIRR131 pKa = 11.84 VASLRR136 pKa = 11.84 QWPAPNAIRR145 pKa = 11.84 VASLRR150 pKa = 11.84 QWPAPNAIRR159 pKa = 11.84 VASLRR164 pKa = 11.84 QWPAPNAIRR173 pKa = 11.84 VASLRR178 pKa = 11.84 QWPAPNAIRR187 pKa = 11.84 VASLRR192 pKa = 11.84 QWPAPNATRR201 pKa = 11.84 VAA203 pKa = 3.73
Molecular weight: 22.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.286
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.618
Grimsley 13.1
Solomon 13.568
Lehninger 13.466
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.568
Sillero 13.056
Patrickios 12.325
IPC_peptide 13.568
IPC2_peptide 12.559
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2999
0
2999
985694
26
2850
328.7
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.257 ± 0.056
0.921 ± 0.014
5.888 ± 0.034
4.872 ± 0.04
3.507 ± 0.032
8.471 ± 0.051
2.509 ± 0.023
5.073 ± 0.029
2.446 ± 0.033
10.287 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.02
2.725 ± 0.029
5.503 ± 0.033
3.463 ± 0.03
7.648 ± 0.046
5.513 ± 0.034
5.646 ± 0.03
7.105 ± 0.037
1.432 ± 0.02
2.228 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here