Streptococcus varani
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2422 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E4CTC7|A0A0E4CTC7_9STRE Sensor histidine kinase OS=Streptococcus varani OX=1608583 GN=BN1356_01945 PE=4 SV=1
MM1 pKa = 7.21 NKK3 pKa = 9.55 SEE5 pKa = 4.06 KK6 pKa = 9.57 MLYY9 pKa = 9.29 TLEE12 pKa = 4.14 QQDD15 pKa = 4.06 LEE17 pKa = 4.15 AAQAYY22 pKa = 9.79 FEE24 pKa = 4.35 EE25 pKa = 5.39 ALLEE29 pKa = 4.61 DD30 pKa = 5.12 DD31 pKa = 5.81 EE32 pKa = 4.57 EE33 pKa = 4.35 TLYY36 pKa = 11.27 DD37 pKa = 3.8 LGTYY41 pKa = 10.14 LEE43 pKa = 4.96 SIGFYY48 pKa = 10.02 PQAKK52 pKa = 9.58 EE53 pKa = 3.77 IYY55 pKa = 8.47 LQLVEE60 pKa = 5.55 DD61 pKa = 4.71 FPEE64 pKa = 4.94 LYY66 pKa = 10.97 LNLAQIANEE75 pKa = 4.15 DD76 pKa = 3.79 GAIEE80 pKa = 3.84 EE81 pKa = 4.17 AFAYY85 pKa = 10.43 LEE87 pKa = 4.76 NISKK91 pKa = 10.56 SSPYY95 pKa = 9.9 YY96 pKa = 10.75 VEE98 pKa = 4.59 ALLVKK103 pKa = 10.46 ADD105 pKa = 4.7 LYY107 pKa = 10.93 QSEE110 pKa = 4.27 GLADD114 pKa = 3.59 VARR117 pKa = 11.84 EE118 pKa = 3.93 KK119 pKa = 11.03 LLEE122 pKa = 4.31 ATSLAEE128 pKa = 5.06 DD129 pKa = 5.08 PILTFGLAEE138 pKa = 4.8 LDD140 pKa = 3.64 MEE142 pKa = 4.9 LGNFKK147 pKa = 10.31 EE148 pKa = 4.63 AIQGYY153 pKa = 7.93 VQLDD157 pKa = 3.22 NRR159 pKa = 11.84 EE160 pKa = 4.08 IYY162 pKa = 11.33 DD163 pKa = 3.53 MVGVSTYY170 pKa = 9.44 QRR172 pKa = 11.84 IGLAYY177 pKa = 9.37 ASLGKK182 pKa = 10.44 FEE184 pKa = 4.18 VAIEE188 pKa = 4.0 FLEE191 pKa = 4.26 KK192 pKa = 10.42 AVEE195 pKa = 4.31 LEE197 pKa = 4.12 YY198 pKa = 11.1 DD199 pKa = 3.55 DD200 pKa = 3.59 QTVYY204 pKa = 11.03 EE205 pKa = 4.77 LATLLYY211 pKa = 10.55 DD212 pKa = 4.58 QEE214 pKa = 4.45 EE215 pKa = 4.44 CQKK218 pKa = 11.38 ANLYY222 pKa = 9.66 FKK224 pKa = 10.63 QLDD227 pKa = 4.07 TINPDD232 pKa = 3.27 FEE234 pKa = 5.47 GYY236 pKa = 10.07 EE237 pKa = 3.83 YY238 pKa = 10.81 AYY240 pKa = 10.38 AQSLHH245 pKa = 6.83 AEE247 pKa = 4.28 HH248 pKa = 7.12 KK249 pKa = 10.46 LDD251 pKa = 3.48 EE252 pKa = 4.52 ALAMVEE258 pKa = 4.29 KK259 pKa = 10.78 GLVKK263 pKa = 10.89 NEE265 pKa = 3.79 FDD267 pKa = 4.74 SVLLLLASQYY277 pKa = 11.4 AYY279 pKa = 10.3 EE280 pKa = 4.12 NHH282 pKa = 7.15 DD283 pKa = 3.5 NQAAEE288 pKa = 4.79 DD289 pKa = 4.01 YY290 pKa = 10.67 LLKK293 pKa = 10.81 AKK295 pKa = 10.31 EE296 pKa = 4.57 DD297 pKa = 3.95 ADD299 pKa = 4.58 DD300 pKa = 3.62 MDD302 pKa = 5.23 EE303 pKa = 4.99 IILRR307 pKa = 11.84 LTNLYY312 pKa = 10.46 LEE314 pKa = 4.39 QEE316 pKa = 4.16 RR317 pKa = 11.84 FDD319 pKa = 4.16 AVVALASDD327 pKa = 4.08 DD328 pKa = 4.13 LDD330 pKa = 3.58 NVLARR335 pKa = 11.84 WNIAKK340 pKa = 10.09 AYY342 pKa = 8.77 QALEE346 pKa = 4.13 EE347 pKa = 4.52 DD348 pKa = 4.48 EE349 pKa = 4.74 KK350 pKa = 11.73 ALDD353 pKa = 4.42 LFDD356 pKa = 3.95 EE357 pKa = 5.03 LKK359 pKa = 10.98 VEE361 pKa = 4.4 LADD364 pKa = 3.94 NPEE367 pKa = 3.78 FLADD371 pKa = 3.52 YY372 pKa = 10.27 VQALKK377 pKa = 10.81 QEE379 pKa = 4.46 GSISEE384 pKa = 3.97 ARR386 pKa = 11.84 EE387 pKa = 3.43 WAHH390 pKa = 7.11 RR391 pKa = 11.84 YY392 pKa = 8.53 LHH394 pKa = 6.6 LVPDD398 pKa = 5.53 DD399 pKa = 3.69 IAMQEE404 pKa = 4.39 FYY406 pKa = 10.99 NQEE409 pKa = 3.93 DD410 pKa = 3.44
Molecular weight: 47.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.732
ProMoST 4.024
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.745
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 4.024
Patrickios 1.214
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|A0A0E3WEJ7|A0A0E3WEJ7_9STRE Phage replication protein OS=Streptococcus varani OX=1608583 GN=BN1356_00167 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.26 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.77 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MATKK25 pKa = 9.7 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.68 GRR39 pKa = 11.84 KK40 pKa = 8.47 VLAVAA45 pKa = 4.94
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2422
0
2422
711518
30
3228
293.8
32.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.585 ± 0.064
0.578 ± 0.015
5.713 ± 0.044
6.865 ± 0.047
4.602 ± 0.042
6.641 ± 0.05
1.977 ± 0.023
7.326 ± 0.053
6.683 ± 0.04
10.062 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.025
4.407 ± 0.04
3.293 ± 0.025
4.187 ± 0.039
4.057 ± 0.035
6.324 ± 0.048
5.571 ± 0.055
6.754 ± 0.039
0.947 ± 0.017
3.811 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here