Streptococcus phage DCC1738
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060QSY2|A0A060QSY2_9CAUD Phage antirepressor protein OS=Streptococcus phage DCC1738 OX=1448273 PE=4 SV=1
MM1 pKa = 8.39 LMFNYY6 pKa = 10.01 DD7 pKa = 3.94 RR8 pKa = 11.84 DD9 pKa = 3.49 IMQPPEE15 pKa = 3.98 EE16 pKa = 4.61 RR17 pKa = 11.84 EE18 pKa = 4.03 EE19 pKa = 4.62 LDD21 pKa = 3.41 PADD24 pKa = 4.33 WIFSAGQWIYY34 pKa = 11.59 VGDD37 pKa = 4.05 CC38 pKa = 3.39
Molecular weight: 4.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 3.732
IPC_protein 3.567
Toseland 3.401
ProMoST 3.795
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.325
Solomon 3.528
Lehninger 3.49
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.706
Patrickios 1.837
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A060QS92|A0A060QS92_9CAUD Phage protein OS=Streptococcus phage DCC1738 OX=1448273 PE=4 SV=1
MM1 pKa = 6.96 QRR3 pKa = 11.84 RR4 pKa = 11.84 EE5 pKa = 3.9 IMNDD9 pKa = 3.93 FIKK12 pKa = 10.46 EE13 pKa = 3.43 IGMAILWMFLGYY25 pKa = 10.88 LLGEE29 pKa = 4.21 RR30 pKa = 11.84 STRR33 pKa = 11.84 GGQIRR38 pKa = 3.63
Molecular weight: 4.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.253
IPC_protein 10.014
Toseland 10.028
ProMoST 10.716
Dawson 10.233
Bjellqvist 9.955
Wikipedia 10.467
Rodwell 10.321
Grimsley 10.321
Solomon 10.394
Lehninger 10.365
Nozaki 9.955
DTASelect 9.97
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.145
Patrickios 10.423
IPC_peptide 10.379
IPC2_peptide 8.799
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
11034
38
2130
197.0
22.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.033 ± 0.703
0.598 ± 0.105
6.272 ± 0.324
8.347 ± 0.712
4.069 ± 0.298
6.543 ± 0.705
1.296 ± 0.186
6.38 ± 0.268
8.637 ± 0.437
8.32 ± 0.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.556 ± 0.302
5.057 ± 0.294
2.529 ± 0.249
4.541 ± 0.296
4.586 ± 0.378
6.154 ± 0.47
5.773 ± 0.485
6.426 ± 0.26
1.314 ± 0.143
3.571 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here