Alces alces faeces associated microvirus MP15 5067
Average proteome isoelectric point is 5.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI08|A0A2Z5CI08_9VIRU Replication initiation protein OS=Alces alces faeces associated microvirus MP15 5067 OX=2219136 PE=4 SV=1
MM1 pKa = 7.27 AVEE4 pKa = 4.38 SKK6 pKa = 10.47 IMRR9 pKa = 11.84 GCCDD13 pKa = 3.17 VFDD16 pKa = 4.58 VSAHH20 pKa = 5.35 YY21 pKa = 8.98 PYY23 pKa = 9.93 EE24 pKa = 4.22 QNVGDD29 pKa = 4.11 YY30 pKa = 10.51 LVEE33 pKa = 4.65 LGTYY37 pKa = 10.29 LPLRR41 pKa = 11.84 KK42 pKa = 9.33 QLEE45 pKa = 4.11 DD46 pKa = 3.48 LKK48 pKa = 11.12 RR49 pKa = 11.84 AGVDD53 pKa = 3.29 LRR55 pKa = 11.84 AQRR58 pKa = 11.84 EE59 pKa = 4.36 ANSLYY64 pKa = 10.34 QAGEE68 pKa = 4.35 TIDD71 pKa = 3.75 LTQEE75 pKa = 4.12 YY76 pKa = 10.52 VDD78 pKa = 4.17 PLQVDD83 pKa = 4.11 EE84 pKa = 5.54 FDD86 pKa = 4.83 LDD88 pKa = 3.93 AKK90 pKa = 10.87 RR91 pKa = 11.84 EE92 pKa = 3.98 LYY94 pKa = 10.67 YY95 pKa = 11.17 RR96 pKa = 11.84 MIVEE100 pKa = 3.94 QEE102 pKa = 3.81 QARR105 pKa = 11.84 KK106 pKa = 9.37 SEE108 pKa = 4.23 RR109 pKa = 11.84 KK110 pKa = 9.29 RR111 pKa = 11.84 RR112 pKa = 11.84 EE113 pKa = 3.58 AEE115 pKa = 3.5 FAEE118 pKa = 4.51 FQKK121 pKa = 10.96 QKK123 pKa = 10.47 AQEE126 pKa = 4.16 SAKK129 pKa = 10.53 KK130 pKa = 10.57 SVDD133 pKa = 3.21 TTEE136 pKa = 4.26 VDD138 pKa = 3.63 SQQ140 pKa = 3.55
Molecular weight: 16.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.805
IPC2_protein 4.825
IPC_protein 4.724
Toseland 4.571
ProMoST 4.813
Dawson 4.66
Bjellqvist 4.8
Wikipedia 4.507
Rodwell 4.558
Grimsley 4.482
Solomon 4.647
Lehninger 4.609
Nozaki 4.762
DTASelect 4.902
Thurlkill 4.571
EMBOSS 4.533
Sillero 4.825
Patrickios 4.024
IPC_peptide 4.66
IPC2_peptide 4.825
IPC2.peptide.svr19 4.806
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ78|A0A2Z5CJ78_9VIRU Major capsid protein OS=Alces alces faeces associated microvirus MP15 5067 OX=2219136 PE=3 SV=1
MM1 pKa = 7.49 SCLSPTFIPVPANKK15 pKa = 10.27 DD16 pKa = 3.4 GVKK19 pKa = 10.41 KK20 pKa = 10.58 LVEE23 pKa = 3.87 NPITHH28 pKa = 6.39 KK29 pKa = 10.85 LEE31 pKa = 3.99 YY32 pKa = 10.91 GMLVPCGKK40 pKa = 10.52 CIPCKK45 pKa = 8.31 VQKK48 pKa = 10.5 ARR50 pKa = 11.84 EE51 pKa = 3.65 WSMRR55 pKa = 11.84 CMLEE59 pKa = 3.77 LPYY62 pKa = 10.08 WYY64 pKa = 10.01 DD65 pKa = 3.36 ADD67 pKa = 4.8 FVTLTYY73 pKa = 10.92 DD74 pKa = 4.88 DD75 pKa = 3.75 EE76 pKa = 4.72 HH77 pKa = 8.34 LPEE80 pKa = 4.89 NGSLVKK86 pKa = 10.26 KK87 pKa = 10.63 DD88 pKa = 3.16 LTDD91 pKa = 3.81 FFKK94 pKa = 10.79 RR95 pKa = 11.84 LRR97 pKa = 11.84 RR98 pKa = 11.84 DD99 pKa = 3.67 LEE101 pKa = 4.11 PLNIKK106 pKa = 10.16 YY107 pKa = 7.73 FACGEE112 pKa = 4.13 YY113 pKa = 10.9 GEE115 pKa = 4.45 NTMRR119 pKa = 11.84 PHH121 pKa = 4.7 YY122 pKa = 9.75 HH123 pKa = 6.96 AIIFGVGLSEE133 pKa = 5.46 RR134 pKa = 11.84 EE135 pKa = 4.11 NKK137 pKa = 10.35 SLGCFYY143 pKa = 11.08 SDD145 pKa = 4.33 NNLIEE150 pKa = 4.96 NNWPYY155 pKa = 11.75 GNVFNGQVTPNSCRR169 pKa = 11.84 YY170 pKa = 7.89 CAEE173 pKa = 4.15 YY174 pKa = 10.96 VFKK177 pKa = 10.77 KK178 pKa = 11.02 YY179 pKa = 10.34 NGKK182 pKa = 10.09 KK183 pKa = 10.14 KK184 pKa = 10.48 IEE186 pKa = 4.18 VYY188 pKa = 10.93 DD189 pKa = 3.83 NLGLQQPFQVQSNGIGKK206 pKa = 10.21 RR207 pKa = 11.84 YY208 pKa = 9.83 LDD210 pKa = 3.98 EE211 pKa = 4.36 NMNDD215 pKa = 3.37 LFQRR219 pKa = 11.84 GYY221 pKa = 10.8 VLYY224 pKa = 10.2 HH225 pKa = 6.08 GKK227 pKa = 9.65 PYY229 pKa = 10.74 SFPRR233 pKa = 11.84 YY234 pKa = 8.91 FADD237 pKa = 3.63 KK238 pKa = 10.55 LKK240 pKa = 11.37 SNDD243 pKa = 3.19 PQRR246 pKa = 11.84 YY247 pKa = 8.82 KK248 pKa = 11.2 DD249 pKa = 3.69 CLDD252 pKa = 3.99 NIKK255 pKa = 10.62 VGSYY259 pKa = 11.05 EE260 pKa = 4.59 DD261 pKa = 3.37 FCEE264 pKa = 4.11 DD265 pKa = 3.33 VKK267 pKa = 10.99 EE268 pKa = 4.42 SEE270 pKa = 4.47 MMSQYY275 pKa = 11.82 DD276 pKa = 3.53 LMQFYY281 pKa = 8.3 KK282 pKa = 10.17 TKK284 pKa = 10.35 KK285 pKa = 9.31 DD286 pKa = 3.29 RR287 pKa = 11.84 EE288 pKa = 4.22 KK289 pKa = 10.87 AIHH292 pKa = 6.9 EE293 pKa = 4.42 DD294 pKa = 3.88 YY295 pKa = 10.78 KK296 pKa = 11.53 SKK298 pKa = 10.58 FYY300 pKa = 11.29 SNRR303 pKa = 11.84 KK304 pKa = 8.28
Molecular weight: 35.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.954
IPC2_protein 7.22
IPC_protein 7.102
Toseland 6.751
ProMoST 7.775
Dawson 7.805
Bjellqvist 8.141
Wikipedia 7.717
Rodwell 7.819
Grimsley 6.781
Solomon 7.849
Lehninger 7.863
Nozaki 8.331
DTASelect 7.922
Thurlkill 7.966
EMBOSS 8.024
Sillero 8.302
Patrickios 3.732
IPC_peptide 7.849
IPC2_peptide 7.249
IPC2.peptide.svr19 7.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1375
140
626
343.8
39.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.4 ± 2.486
1.382 ± 0.692
7.418 ± 0.636
6.764 ± 1.254
5.091 ± 1.174
5.527 ± 0.948
2.036 ± 0.241
3.709 ± 0.525
5.745 ± 1.627
7.636 ± 0.33
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.618 ± 0.259
5.6 ± 0.763
3.927 ± 0.794
4.727 ± 1.147
5.673 ± 0.439
7.127 ± 1.154
5.818 ± 1.176
6.255 ± 0.347
0.727 ± 0.22
5.818 ± 0.911
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here