Streptococcus satellite phage Javan285

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIM1|A0A4D5ZIM1_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan285 OX=2558607 GN=JavanS285_0001 PE=3 SV=1
MM1 pKa = 7.61TNTNKK6 pKa = 7.94TTAPKK11 pKa = 9.89TLTEE15 pKa = 4.16LKK17 pKa = 10.37AWTKK21 pKa = 7.97EE22 pKa = 3.45ARR24 pKa = 11.84NWVEE28 pKa = 3.69YY29 pKa = 10.69LQDD32 pKa = 3.71EE33 pKa = 4.74YY34 pKa = 11.06HH35 pKa = 7.11GYY37 pKa = 8.76MPTGHH42 pKa = 6.14QEE44 pKa = 3.99AFGSITNRR52 pKa = 11.84IYY54 pKa = 11.35NCLDD58 pKa = 3.54CLTEE62 pKa = 4.1MFDD65 pKa = 3.92NDD67 pKa = 3.45GLEE70 pKa = 4.1LVEE73 pKa = 4.13YY74 pKa = 9.74TIDD77 pKa = 3.72PQEE80 pKa = 3.98TMIDD84 pKa = 3.57DD85 pKa = 3.88NQANEE90 pKa = 3.79MAQYY94 pKa = 10.55NEE96 pKa = 5.46LMAQYY101 pKa = 10.41NEE103 pKa = 3.98LMKK106 pKa = 10.63EE107 pKa = 4.13VEE109 pKa = 4.64DD110 pKa = 3.69SDD112 pKa = 4.32KK113 pKa = 10.63EE114 pKa = 4.14ARR116 pKa = 11.84EE117 pKa = 3.61IAYY120 pKa = 10.24GDD122 pKa = 4.16TIDD125 pKa = 5.02KK126 pKa = 11.06YY127 pKa = 9.96IDD129 pKa = 3.23KK130 pKa = 10.33RR131 pKa = 11.84AKK133 pKa = 8.97QLKK136 pKa = 8.43EE137 pKa = 3.37QATFDD142 pKa = 3.8EE143 pKa = 5.04LVDD146 pKa = 3.93KK147 pKa = 11.06VALYY151 pKa = 10.43EE152 pKa = 4.89SEE154 pKa = 4.11LLDD157 pKa = 3.57YY158 pKa = 11.28AEE160 pKa = 5.48RR161 pKa = 11.84LLSDD165 pKa = 4.64DD166 pKa = 4.48PLIADD171 pKa = 3.97SEE173 pKa = 4.7TAIGTLEE180 pKa = 3.99MLDD183 pKa = 3.96NEE185 pKa = 5.27AIDD188 pKa = 4.13LFKK191 pKa = 11.29SLDD194 pKa = 3.42IDD196 pKa = 3.65NEE198 pKa = 4.42YY199 pKa = 10.91QGLEE203 pKa = 4.14YY204 pKa = 11.01YY205 pKa = 8.17DD206 pKa = 3.56TSLNKK211 pKa = 9.96EE212 pKa = 4.17DD213 pKa = 3.66

Molecular weight: 24.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNB4|A0A4D5ZNB4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan285 OX=2558607 GN=JavanS285_0009 PE=4 SV=1
MM1 pKa = 7.39FLHH4 pKa = 6.8FPIILDD10 pKa = 4.48GIIEE14 pKa = 4.21ADD16 pKa = 3.38EE17 pKa = 4.25TFFAISYY24 pKa = 9.35KK25 pKa = 10.68GNHH28 pKa = 6.13SKK30 pKa = 10.92SKK32 pKa = 8.95TFAMPRR38 pKa = 11.84KK39 pKa = 8.88AYY41 pKa = 9.67KK42 pKa = 9.82RR43 pKa = 11.84GHH45 pKa = 4.97STHH48 pKa = 6.36IRR50 pKa = 11.84GLSQEE55 pKa = 4.4KK56 pKa = 10.23VCVPCAVNRR65 pKa = 11.84NGLSISKK72 pKa = 8.26ITNTGRR78 pKa = 11.84VSTRR82 pKa = 11.84DD83 pKa = 3.09LHH85 pKa = 7.18HH86 pKa = 7.14IYY88 pKa = 10.55DD89 pKa = 4.01GRR91 pKa = 11.84IKK93 pKa = 10.08TNSTLVTDD101 pKa = 4.3KK102 pKa = 10.3MNSYY106 pKa = 10.45VRR108 pKa = 11.84FTNANGIDD116 pKa = 4.04LVQLKK121 pKa = 8.99TGKK124 pKa = 9.91AKK126 pKa = 10.54KK127 pKa = 10.1GIYY130 pKa = 9.6NIQHH134 pKa = 5.91INSYY138 pKa = 10.3HH139 pKa = 5.54SQLKK143 pKa = 9.97RR144 pKa = 11.84FMRR147 pKa = 11.84GFNGVSTKK155 pKa = 10.69YY156 pKa = 10.69LNNYY160 pKa = 7.83LVWNNLVNYY169 pKa = 9.92AKK171 pKa = 10.47EE172 pKa = 3.76SDD174 pKa = 3.53MEE176 pKa = 4.07KK177 pKa = 10.79RR178 pKa = 11.84NIFLTFVLATLKK190 pKa = 9.45TAKK193 pKa = 10.3CRR195 pKa = 11.84DD196 pKa = 3.51LSNRR200 pKa = 11.84PAVPLVAA207 pKa = 5.64

Molecular weight: 23.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2731

52

468

170.7

19.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.163 ± 0.541

0.696 ± 0.211

6.225 ± 0.718

8.275 ± 0.875

3.881 ± 0.368

4.76 ± 0.322

2.417 ± 0.594

7.653 ± 0.549

8.825 ± 0.545

9.667 ± 0.711

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.254

5.785 ± 0.524

2.673 ± 0.344

3.881 ± 0.416

5.126 ± 0.611

4.65 ± 0.446

6.518 ± 0.47

5.419 ± 0.428

0.952 ± 0.16

5.016 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski