Opisthocomus hoazin (Hoatzin) (Phasianus hoazin)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12773 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091X7Y6|A0A091X7Y6_OPIHO Small G protein signaling modulator 2 (Fragment) OS=Opisthocomus hoazin OX=30419 GN=N306_15615 PE=4 SV=1
SS1 pKa = 6.77 RR2 pKa = 11.84 LSWLEE7 pKa = 3.48 KK8 pKa = 10.42 SGVGFSLDD16 pKa = 3.46 YY17 pKa = 9.24 PTISLHH23 pKa = 6.08 AVSRR27 pKa = 11.84 DD28 pKa = 3.11 LSAYY32 pKa = 8.42 PCEE35 pKa = 4.18 HH36 pKa = 7.0 LYY38 pKa = 11.68 VMVNAKK44 pKa = 10.31 FEE46 pKa = 4.15 EE47 pKa = 4.78 EE48 pKa = 4.13 EE49 pKa = 4.25 TKK51 pKa = 10.06 EE52 pKa = 3.96 APMAEE57 pKa = 4.28 GEE59 pKa = 4.22 EE60 pKa = 4.57 EE61 pKa = 4.21 EE62 pKa = 6.05 DD63 pKa = 4.02 SDD65 pKa = 6.23 DD66 pKa = 3.89 DD67 pKa = 4.15 VEE69 pKa = 5.15 PIAEE73 pKa = 4.09 FRR75 pKa = 11.84 FVPSDD80 pKa = 3.33 KK81 pKa = 10.82 SALDD85 pKa = 3.45 AMFSAMCEE93 pKa = 4.27 CQALHH98 pKa = 7.41 PDD100 pKa = 4.21 PDD102 pKa = 5.77 DD103 pKa = 4.05 EE104 pKa = 7.61 DD105 pKa = 4.46 SDD107 pKa = 3.76 NDD109 pKa = 3.97 YY110 pKa = 11.37 EE111 pKa = 4.59 GEE113 pKa = 4.16 EE114 pKa = 4.41 YY115 pKa = 10.56 DD116 pKa = 4.4 VEE118 pKa = 4.23 AHH120 pKa = 6.13 EE121 pKa = 5.43 LEE123 pKa = 4.54 QGDD126 pKa = 3.63 IPTFYY131 pKa = 10.0 TYY133 pKa = 11.09 EE134 pKa = 4.17 EE135 pKa = 4.62 GLSHH139 pKa = 6.9 LTAEE143 pKa = 4.71 GQATLEE149 pKa = 4.11 RR150 pKa = 11.84 LEE152 pKa = 4.74 GMLAQSVSSQYY163 pKa = 11.66 NMAGVRR169 pKa = 11.84 TEE171 pKa = 3.7 DD172 pKa = 3.58 SIRR175 pKa = 11.84 EE176 pKa = 4.1 FEE178 pKa = 5.27 DD179 pKa = 3.49 GMEE182 pKa = 4.04 VDD184 pKa = 3.86 VAPVVAGQFEE194 pKa = 4.42 DD195 pKa = 5.26 AEE197 pKa = 4.32 VDD199 pKa = 3.59 HH200 pKa = 7.08
Molecular weight: 22.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.605
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.63
Grimsley 3.516
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.024
Thurlkill 3.63
EMBOSS 3.656
Sillero 3.91
Patrickios 1.837
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A091VA41|A0A091VA41_OPIHO Tetratricopeptide repeat protein 38 (Fragment) OS=Opisthocomus hoazin OX=30419 GN=N306_08777 PE=3 SV=1
KKK2 pKa = 10.12 RR3 pKa = 11.84 QGKKK7 pKa = 8.85 RR8 pKa = 11.84 QGKKK12 pKa = 8.73 RR13 pKa = 11.84 QGKKK17 pKa = 8.73 RR18 pKa = 11.84 QGKKK22 pKa = 8.73 RR23 pKa = 11.84 QGKKK27 pKa = 8.73 RR28 pKa = 11.84 QGKKK32 pKa = 8.73 RR33 pKa = 11.84 QGKKK37 pKa = 8.63 RR38 pKa = 11.84 QGNSRR43 pKa = 11.84 QGKKK47 pKa = 7.87 RR48 pKa = 11.84 KKK50 pKa = 9.33 KKK52 pKa = 10.31 RR53 pKa = 11.84
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.519
IPC2_protein 11.272
IPC_protein 12.837
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.822
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.544
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12773
0
12773
5377037
32
5562
421.0
47.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.422 ± 0.019
2.278 ± 0.018
5.06 ± 0.016
7.169 ± 0.028
3.964 ± 0.016
5.87 ± 0.029
2.541 ± 0.012
4.986 ± 0.016
6.502 ± 0.026
9.751 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.238 ± 0.01
4.138 ± 0.014
5.147 ± 0.026
4.601 ± 0.02
5.284 ± 0.016
8.132 ± 0.03
5.352 ± 0.014
6.368 ± 0.019
1.21 ± 0.007
2.984 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here