Flavobacterium cyanobacteriorum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A256A2H5|A0A256A2H5_9FLAO Uncharacterized protein OS=Flavobacterium cyanobacteriorum OX=2022802 GN=CHU92_00935 PE=4 SV=1
MM1 pKa = 7.5ALSVYY6 pKa = 10.23VISCSPDD13 pKa = 3.24NNNGEE18 pKa = 4.28APPRR22 pKa = 11.84DD23 pKa = 3.75YY24 pKa = 11.34AVQYY28 pKa = 10.86ASEE31 pKa = 4.28KK32 pKa = 10.92ADD34 pKa = 3.23IEE36 pKa = 4.27NFLKK40 pKa = 9.1KK41 pKa = 9.17TYY43 pKa = 9.68MVVDD47 pKa = 3.89EE48 pKa = 4.59TTWDD52 pKa = 3.63VVIDD56 pKa = 4.3SIGPDD61 pKa = 3.56TPQVSIWDD69 pKa = 3.55QTEE72 pKa = 3.84YY73 pKa = 10.72PLQQKK78 pKa = 9.25IVRR81 pKa = 11.84SNNVDD86 pKa = 3.22YY87 pKa = 10.03TVYY90 pKa = 10.92YY91 pKa = 8.76ITFNEE96 pKa = 4.92GVGSAPIKK104 pKa = 10.29ADD106 pKa = 3.12NVFISYY112 pKa = 10.37RR113 pKa = 11.84GIRR116 pKa = 11.84LDD118 pKa = 3.74TEE120 pKa = 3.87QFTYY124 pKa = 11.03VPFPANYY131 pKa = 9.9SSLLSTIEE139 pKa = 3.79GWQEE143 pKa = 3.66IIPLFKK149 pKa = 10.86AGIEE153 pKa = 4.51TNTNPQDD160 pKa = 3.51PASFSDD166 pKa = 3.7YY167 pKa = 10.81GAGVMFLPSGLGYY180 pKa = 10.92YY181 pKa = 10.23NISRR185 pKa = 11.84VGIPSYY191 pKa = 11.14SSLIFSFKK199 pKa = 10.61LFAIQRR205 pKa = 11.84ADD207 pKa = 3.39NDD209 pKa = 3.81RR210 pKa = 11.84DD211 pKa = 3.62GVLSVNEE218 pKa = 4.1TGGFADD224 pKa = 4.2IDD226 pKa = 4.71DD227 pKa = 4.7YY228 pKa = 12.15DD229 pKa = 5.78SDD231 pKa = 5.52DD232 pKa = 5.65DD233 pKa = 6.12DD234 pKa = 3.53IANYY238 pKa = 10.41RR239 pKa = 11.84DD240 pKa = 3.39VDD242 pKa = 4.04DD243 pKa = 6.03DD244 pKa = 4.35NDD246 pKa = 4.9GILTKK251 pKa = 10.42NEE253 pKa = 3.94KK254 pKa = 10.17QGTGSPEE261 pKa = 4.81DD262 pKa = 4.59LDD264 pKa = 4.01TDD266 pKa = 3.4NDD268 pKa = 4.81GIKK271 pKa = 10.58NYY273 pKa = 10.55LDD275 pKa = 3.71TDD277 pKa = 4.13DD278 pKa = 6.02DD279 pKa = 5.19GDD281 pKa = 4.6GIPTKK286 pKa = 10.8DD287 pKa = 3.91EE288 pKa = 4.54LNLPPCHH295 pKa = 6.68NNPAVPRR302 pKa = 11.84YY303 pKa = 10.02LNSSCC308 pKa = 5.02

Molecular weight: 34.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A256A801|A0A256A801_9FLAO HTH araC/xylS-type domain-containing protein OS=Flavobacterium cyanobacteriorum OX=2022802 GN=CHU92_00355 PE=4 SV=1
AA1 pKa = 7.44LRR3 pKa = 11.84AFRR6 pKa = 11.84CNPSRR11 pKa = 11.84GVGGEE16 pKa = 3.73RR17 pKa = 11.84GVGSFHH23 pKa = 8.0KK24 pKa = 10.68YY25 pKa = 9.44LLKK28 pKa = 10.65ININPLYY35 pKa = 9.17IWFVVLEE42 pKa = 4.29IPSGFPHH49 pKa = 6.81NAPAGLPTASSSPPSGRR66 pKa = 11.84GCRR69 pKa = 11.84PHH71 pKa = 6.29HH72 pKa = 5.92TAFLSPIAPPP82 pKa = 3.62

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2995

0

2995

945582

29

2789

315.7

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.258 ± 0.047

0.799 ± 0.016

5.244 ± 0.034

6.357 ± 0.067

5.128 ± 0.041

6.653 ± 0.052

1.779 ± 0.025

7.469 ± 0.042

7.21 ± 0.083

9.12 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.029

5.873 ± 0.056

3.736 ± 0.032

3.468 ± 0.025

4.004 ± 0.033

6.024 ± 0.044

5.978 ± 0.088

6.425 ± 0.032

1.011 ± 0.019

4.235 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski