Amino acid dipepetide frequency for Flavobacterium cyanobacteriorum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.956AlaAla: 5.956 ± 0.097	0.651AlaCys: 0.651 ± 0.035	3.966AlaAsp: 3.966 ± 0.074	4.841AlaGlu: 4.841 ± 0.082	3.494AlaPhe: 3.494 ± 0.068	5.648AlaGly: 5.648 ± 0.103	1.162AlaHis: 1.162 ± 0.038	5.382AlaIle: 5.382 ± 0.081	4.701AlaLys: 4.701 ± 0.095	6.799AlaLeu: 6.799 ± 0.091	1.685AlaMet: 1.685 ± 0.045	3.457AlaAsn: 3.457 ± 0.078	2.372AlaPro: 2.372 ± 0.062	2.667AlaGln: 2.667 ± 0.053	2.751AlaArg: 2.751 ± 0.061	4.262AlaSer: 4.262 ± 0.084	4.37AlaThr: 4.37 ± 0.108	5.033AlaVal: 5.033 ± 0.071	0.703AlaTrp: 0.703 ± 0.029	2.677AlaTyr: 2.677 ± 0.062	0.003AlaXaa: 0.003 ± 0.002
Cys	0.491CysAla: 0.491 ± 0.026	0.106CysCys: 0.106 ± 0.01	0.431CysAsp: 0.431 ± 0.023	0.427CysGlu: 0.427 ± 0.023	0.448CysPhe: 0.448 ± 0.024	0.701CysGly: 0.701 ± 0.036	0.186CysHis: 0.186 ± 0.015	0.608CysIle: 0.608 ± 0.028	0.452CysLys: 0.452 ± 0.021	0.672CysLeu: 0.672 ± 0.028	0.15CysMet: 0.15 ± 0.012	0.455CysAsn: 0.455 ± 0.024	0.35CysPro: 0.35 ± 0.02	0.224CysGln: 0.224 ± 0.016	0.339CysArg: 0.339 ± 0.02	0.63CysSer: 0.63 ± 0.029	0.496CysThr: 0.496 ± 0.037	0.451CysVal: 0.451 ± 0.021	0.06CysTrp: 0.06 ± 0.008	0.313CysTyr: 0.313 ± 0.019	0.002CysXaa: 0.002 ± 0.001
Asp	3.763AspAla: 3.763 ± 0.069	0.396AspCys: 0.396 ± 0.02	2.714AspAsp: 2.714 ± 0.062	3.593AspGlu: 3.593 ± 0.069	3.392AspPhe: 3.392 ± 0.056	3.345AspGly: 3.345 ± 0.069	0.753AspHis: 0.753 ± 0.031	4.359AspIle: 4.359 ± 0.072	4.03AspLys: 4.03 ± 0.084	4.246AspLeu: 4.246 ± 0.072	1.164AspMet: 1.164 ± 0.03	3.287AspAsn: 3.287 ± 0.057	1.673AspPro: 1.673 ± 0.046	1.124AspGln: 1.124 ± 0.037	2.119AspArg: 2.119 ± 0.052	2.719AspSer: 2.719 ± 0.061	2.93AspThr: 2.93 ± 0.054	3.467AspVal: 3.467 ± 0.064	0.643AspTrp: 0.643 ± 0.025	2.726AspTyr: 2.726 ± 0.052	0.001AspXaa: 0.001 ± 0.001
Glu	4.803GluAla: 4.803 ± 0.08	0.353GluCys: 0.353 ± 0.023	3.195GluAsp: 3.195 ± 0.069	4.689GluGlu: 4.689 ± 0.097	2.789GluPhe: 2.789 ± 0.06	4.0GluGly: 4.0 ± 0.079	1.145GluHis: 1.145 ± 0.037	4.886GluIle: 4.886 ± 0.086	5.681GluLys: 5.681 ± 0.092	5.768GluLeu: 5.768 ± 0.09	1.546GluMet: 1.546 ± 0.046	4.04GluAsn: 4.04 ± 0.07	1.818GluPro: 1.818 ± 0.043	2.518GluGln: 2.518 ± 0.058	2.677GluArg: 2.677 ± 0.06	2.869GluSer: 2.869 ± 0.05	3.431GluThr: 3.431 ± 0.055	4.198GluVal: 4.198 ± 0.072	0.639GluTrp: 0.639 ± 0.028	2.525GluTyr: 2.525 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.292PheAla: 3.292 ± 0.067	0.504PheCys: 0.504 ± 0.024	3.118PheAsp: 3.118 ± 0.06	3.283PheGlu: 3.283 ± 0.064	2.741PhePhe: 2.741 ± 0.066	3.567PheGly: 3.567 ± 0.065	0.814PheHis: 0.814 ± 0.029	3.918PheIle: 3.918 ± 0.081	3.035PheLys: 3.035 ± 0.065	4.408PheLeu: 4.408 ± 0.086	1.194PheMet: 1.194 ± 0.038	3.183PheAsn: 3.183 ± 0.073	1.727PhePro: 1.727 ± 0.043	1.242PheGln: 1.242 ± 0.035	2.272PheArg: 2.272 ± 0.048	3.847PheSer: 3.847 ± 0.074	3.579PheThr: 3.579 ± 0.087	2.831PheVal: 2.831 ± 0.057	0.52PheTrp: 0.52 ± 0.022	2.203PheTyr: 2.203 ± 0.044	0.001PheXaa: 0.001 ± 0.001
Gly	4.412GlyAla: 4.412 ± 0.083	0.719GlyCys: 0.719 ± 0.037	3.065GlyAsp: 3.065 ± 0.067	3.669GlyGlu: 3.669 ± 0.07	3.62GlyPhe: 3.62 ± 0.074	4.606GlyGly: 4.606 ± 0.107	1.276GlyHis: 1.276 ± 0.043	5.441GlyIle: 5.441 ± 0.086	4.837GlyLys: 4.837 ± 0.087	5.965GlyLeu: 5.965 ± 0.095	1.611GlyMet: 1.611 ± 0.047	3.941GlyAsn: 3.941 ± 0.103	1.517GlyPro: 1.517 ± 0.06	2.27GlyGln: 2.27 ± 0.052	2.786GlyArg: 2.786 ± 0.061	4.246GlySer: 4.246 ± 0.09	4.634GlyThr: 4.634 ± 0.141	4.117GlyVal: 4.117 ± 0.081	0.8GlyTrp: 0.8 ± 0.031	2.994GlyTyr: 2.994 ± 0.064	0.001GlyXaa: 0.001 ± 0.001
His	1.046HisAla: 1.046 ± 0.036	0.179HisCys: 0.179 ± 0.014	0.886HisAsp: 0.886 ± 0.032	0.997HisGlu: 0.997 ± 0.033	1.092HisPhe: 1.092 ± 0.035	1.06HisGly: 1.06 ± 0.04	0.482HisHis: 0.482 ± 0.028	1.37HisIle: 1.37 ± 0.039	1.231HisLys: 1.231 ± 0.044	1.747HisLeu: 1.747 ± 0.042	0.347HisMet: 0.347 ± 0.017	1.049HisAsn: 1.049 ± 0.033	0.86HisPro: 0.86 ± 0.037	0.67HisGln: 0.67 ± 0.025	0.773HisArg: 0.773 ± 0.029	0.977HisSer: 0.977 ± 0.033	0.999HisThr: 0.999 ± 0.034	0.935HisVal: 0.935 ± 0.035	0.195HisTrp: 0.195 ± 0.016	0.897HisTyr: 0.897 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.76IleAla: 5.76 ± 0.091	0.603IleCys: 0.603 ± 0.024	4.32IleAsp: 4.32 ± 0.077	4.685IleGlu: 4.685 ± 0.092	3.437IlePhe: 3.437 ± 0.068	4.484IleGly: 4.484 ± 0.081	1.297IleHis: 1.297 ± 0.037	5.941IleIle: 5.941 ± 0.104	5.361IleLys: 5.361 ± 0.096	6.307IleLeu: 6.307 ± 0.107	1.494IleMet: 1.494 ± 0.047	4.531IleAsn: 4.531 ± 0.076	3.113IlePro: 3.113 ± 0.056	2.377IleGln: 2.377 ± 0.053	2.816IleArg: 2.816 ± 0.058	5.016IleSer: 5.016 ± 0.076	5.109IleThr: 5.109 ± 0.098	4.588IleVal: 4.588 ± 0.084	0.584IleTrp: 0.584 ± 0.022	2.861IleTyr: 2.861 ± 0.053	0.001IleXaa: 0.001 ± 0.001
Lys	5.475LysAla: 5.475 ± 0.082	0.334LysCys: 0.334 ± 0.02	4.106LysAsp: 4.106 ± 0.09	5.416LysGlu: 5.416 ± 0.097	2.854LysPhe: 2.854 ± 0.065	4.492LysGly: 4.492 ± 0.084	1.237LysHis: 1.237 ± 0.036	5.538LysIle: 5.538 ± 0.087	6.431LysLys: 6.431 ± 0.125	6.067LysLeu: 6.067 ± 0.101	1.926LysMet: 1.926 ± 0.049	4.487LysAsn: 4.487 ± 0.094	2.613LysPro: 2.613 ± 0.066	2.686LysGln: 2.686 ± 0.068	2.707LysArg: 2.707 ± 0.055	3.752LysSer: 3.752 ± 0.06	3.915LysThr: 3.915 ± 0.075	4.45LysVal: 4.45 ± 0.087	0.698LysTrp: 0.698 ± 0.03	2.911LysTyr: 2.911 ± 0.056	0.006LysXaa: 0.006 ± 0.003
Leu	6.332LeuAla: 6.332 ± 0.086	0.742LeuCys: 0.742 ± 0.033	4.608LeuAsp: 4.608 ± 0.085	5.682LeuGlu: 5.682 ± 0.097	4.827LeuPhe: 4.827 ± 0.091	5.534LeuGly: 5.534 ± 0.096	1.742LeuHis: 1.742 ± 0.05	6.027LeuIle: 6.027 ± 0.109	6.99LeuLys: 6.99 ± 0.106	9.288LeuLeu: 9.288 ± 0.151	2.068LeuMet: 2.068 ± 0.053	5.113LeuAsn: 5.113 ± 0.085	3.961LeuPro: 3.961 ± 0.064	3.499LeuGln: 3.499 ± 0.064	3.63LeuArg: 3.63 ± 0.064	6.284LeuSer: 6.284 ± 0.079	5.24LeuThr: 5.24 ± 0.083	5.294LeuVal: 5.294 ± 0.09	0.831LeuTrp: 0.831 ± 0.031	3.5LeuTyr: 3.5 ± 0.075	0.003LeuXaa: 0.003 ± 0.002
Met	1.943MetAla: 1.943 ± 0.048	0.136MetCys: 0.136 ± 0.011	1.055MetAsp: 1.055 ± 0.031	1.476MetGlu: 1.476 ± 0.043	0.898MetPhe: 0.898 ± 0.034	1.386MetGly: 1.386 ± 0.041	0.436MetHis: 0.436 ± 0.022	1.401MetIle: 1.401 ± 0.042	2.195MetLys: 2.195 ± 0.051	2.077MetLeu: 2.077 ± 0.054	0.601MetMet: 0.601 ± 0.031	1.132MetAsn: 1.132 ± 0.034	0.984MetPro: 0.984 ± 0.036	0.904MetGln: 0.904 ± 0.029	0.957MetArg: 0.957 ± 0.036	1.276MetSer: 1.276 ± 0.039	1.052MetThr: 1.052 ± 0.035	1.378MetVal: 1.378 ± 0.04	0.172MetTrp: 0.172 ± 0.015	0.792MetTyr: 0.792 ± 0.029	0.001MetXaa: 0.001 ± 0.001
Asn	3.991AsnAla: 3.991 ± 0.069	0.462AsnCys: 0.462 ± 0.033	2.898AsnAsp: 2.898 ± 0.059	3.353AsnGlu: 3.353 ± 0.063	3.061AsnPhe: 3.061 ± 0.068	4.047AsnGly: 4.047 ± 0.104	0.974AsnHis: 0.974 ± 0.035	4.655AsnIle: 4.655 ± 0.073	3.752AsnLys: 3.752 ± 0.078	4.965AsnLeu: 4.965 ± 0.096	1.149AsnMet: 1.149 ± 0.041	3.928AsnAsn: 3.928 ± 0.099	3.031AsnPro: 3.031 ± 0.07	1.83AsnGln: 1.83 ± 0.043	2.577AsnArg: 2.577 ± 0.056	3.485AsnSer: 3.485 ± 0.065	3.686AsnThr: 3.686 ± 0.098	3.493AsnVal: 3.493 ± 0.076	0.669AsnTrp: 0.669 ± 0.029	2.726AsnTyr: 2.726 ± 0.077	0.001AsnXaa: 0.001 ± 0.001
Pro	3.058ProAla: 3.058 ± 0.082	0.245ProCys: 0.245 ± 0.016	2.31ProAsp: 2.31 ± 0.055	3.027ProGlu: 3.027 ± 0.067	1.986ProPhe: 1.986 ± 0.046	2.751ProGly: 2.751 ± 0.076	0.665ProHis: 0.665 ± 0.028	2.063ProIle: 2.063 ± 0.051	2.349ProLys: 2.349 ± 0.052	3.267ProLeu: 3.267 ± 0.065	0.73ProMet: 0.73 ± 0.03	1.98ProAsn: 1.98 ± 0.058	1.126ProPro: 1.126 ± 0.042	1.419ProGln: 1.419 ± 0.04	1.139ProArg: 1.139 ± 0.035	2.099ProSer: 2.099 ± 0.054	1.858ProThr: 1.858 ± 0.056	3.355ProVal: 3.355 ± 0.07	0.326ProTrp: 0.326 ± 0.021	1.606ProTyr: 1.606 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	2.475GlnAla: 2.475 ± 0.058	0.22GlnCys: 0.22 ± 0.016	1.667GlnAsp: 1.667 ± 0.045	2.271GlnGlu: 2.271 ± 0.056	1.611GlnPhe: 1.611 ± 0.046	1.95GlnGly: 1.95 ± 0.042	0.672GlnHis: 0.672 ± 0.027	2.177GlnIle: 2.177 ± 0.055	2.718GlnLys: 2.718 ± 0.063	3.593GlnLeu: 3.593 ± 0.073	0.857GlnMet: 0.857 ± 0.029	2.074GlnAsn: 2.074 ± 0.055	1.386GlnPro: 1.386 ± 0.046	1.72GlnGln: 1.72 ± 0.049	1.371GlnArg: 1.371 ± 0.038	1.857GlnSer: 1.857 ± 0.045	1.764GlnThr: 1.764 ± 0.052	2.31GlnVal: 2.31 ± 0.045	0.407GlnTrp: 0.407 ± 0.02	1.582GlnTyr: 1.582 ± 0.047	0.001GlnXaa: 0.001 ± 0.001
Arg	2.461ArgAla: 2.461 ± 0.051	0.254ArgCys: 0.254 ± 0.018	2.001ArgAsp: 2.001 ± 0.047	2.78ArgGlu: 2.78 ± 0.066	2.174ArgPhe: 2.174 ± 0.05	2.167ArgGly: 2.167 ± 0.052	0.871ArgHis: 0.871 ± 0.028	3.138ArgIle: 3.138 ± 0.055	3.11ArgLys: 3.11 ± 0.067	3.933ArgLeu: 3.933 ± 0.075	1.094ArgMet: 1.094 ± 0.031	2.452ArgAsn: 2.452 ± 0.054	1.368ArgPro: 1.368 ± 0.041	1.634ArgGln: 1.634 ± 0.042	1.712ArgArg: 1.712 ± 0.044	2.039ArgSer: 2.039 ± 0.046	2.004ArgThr: 2.004 ± 0.041	2.511ArgVal: 2.511 ± 0.054	0.458ArgTrp: 0.458 ± 0.022	1.873ArgTyr: 1.873 ± 0.046	0.002ArgXaa: 0.002 ± 0.001
Ser	4.349SerAla: 4.349 ± 0.082	0.635SerCys: 0.635 ± 0.03	2.787SerAsp: 2.787 ± 0.053	3.266SerGlu: 3.266 ± 0.067	3.612SerPhe: 3.612 ± 0.069	5.02SerGly: 5.02 ± 0.108	1.059SerHis: 1.059 ± 0.033	4.317SerIle: 4.317 ± 0.081	3.729SerLys: 3.729 ± 0.074	5.751SerLeu: 5.751 ± 0.081	1.214SerMet: 1.214 ± 0.039	3.318SerAsn: 3.318 ± 0.077	2.278SerPro: 2.278 ± 0.056	2.038SerGln: 2.038 ± 0.047	2.478SerArg: 2.478 ± 0.057	3.742SerSer: 3.742 ± 0.092	3.296SerThr: 3.296 ± 0.086	4.035SerVal: 4.035 ± 0.076	0.63SerTrp: 0.63 ± 0.027	2.685SerTyr: 2.685 ± 0.054	0.003SerXaa: 0.003 ± 0.002
Thr	4.782ThrAla: 4.782 ± 0.126	0.437ThrCys: 0.437 ± 0.028	3.296ThrAsp: 3.296 ± 0.062	3.385ThrGlu: 3.385 ± 0.058	3.008ThrPhe: 3.008 ± 0.073	5.086ThrGly: 5.086 ± 0.103	0.955ThrHis: 0.955 ± 0.033	4.704ThrIle: 4.704 ± 0.102	3.292ThrLys: 3.292 ± 0.063	5.488ThrLeu: 5.488 ± 0.083	0.955ThrMet: 0.955 ± 0.034	3.08ThrAsn: 3.08 ± 0.091	2.768ThrPro: 2.768 ± 0.072	1.955ThrGln: 1.955 ± 0.066	1.977ThrArg: 1.977 ± 0.039	3.491ThrSer: 3.491 ± 0.094	3.77ThrThr: 3.77 ± 0.117	4.393ThrVal: 4.393 ± 0.094	0.582ThrTrp: 0.582 ± 0.03	2.378ThrTyr: 2.378 ± 0.06	0.001ThrXaa: 0.001 ± 0.001
Val	4.898ValAla: 4.898 ± 0.081	0.565ValCys: 0.565 ± 0.026	3.191ValAsp: 3.191 ± 0.065	3.717ValGlu: 3.717 ± 0.082	3.156ValPhe: 3.156 ± 0.064	3.512ValGly: 3.512 ± 0.062	1.039ValHis: 1.039 ± 0.032	4.966ValIle: 4.966 ± 0.072	4.533ValLys: 4.533 ± 0.08	6.02ValLeu: 6.02 ± 0.083	1.397ValMet: 1.397 ± 0.043	3.782ValAsn: 3.782 ± 0.08	2.622ValPro: 2.622 ± 0.058	2.028ValGln: 2.028 ± 0.053	2.419ValArg: 2.419 ± 0.054	4.316ValSer: 4.316 ± 0.075	4.402ValThr: 4.402 ± 0.125	4.465ValVal: 4.465 ± 0.084	0.606ValTrp: 0.606 ± 0.024	2.615ValTyr: 2.615 ± 0.053	0.001ValXaa: 0.001 ± 0.001
Trp	0.672TrpAla: 0.672 ± 0.03	0.1TrpCys: 0.1 ± 0.01	0.584TrpAsp: 0.584 ± 0.028	0.62TrpGlu: 0.62 ± 0.024	0.587TrpPhe: 0.587 ± 0.025	0.637TrpGly: 0.637 ± 0.031	0.234TrpHis: 0.234 ± 0.016	0.734TrpIle: 0.734 ± 0.031	0.748TrpLys: 0.748 ± 0.027	0.987TrpLeu: 0.987 ± 0.039	0.272TrpMet: 0.272 ± 0.017	0.665TrpAsn: 0.665 ± 0.03	0.217TrpPro: 0.217 ± 0.017	0.427TrpGln: 0.427 ± 0.025	0.414TrpArg: 0.414 ± 0.021	0.539TrpSer: 0.539 ± 0.024	0.489TrpThr: 0.489 ± 0.025	0.615TrpVal: 0.615 ± 0.027	0.135TrpTrp: 0.135 ± 0.013	0.437TrpTyr: 0.437 ± 0.02	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.622TyrAla: 2.622 ± 0.053	0.349TyrCys: 0.349 ± 0.019	2.247TyrAsp: 2.247 ± 0.051	2.385TyrGlu: 2.385 ± 0.052	2.495TyrPhe: 2.495 ± 0.055	2.531TyrGly: 2.531 ± 0.06	0.793TyrHis: 0.793 ± 0.033	3.053TyrIle: 3.053 ± 0.059	2.928TyrLys: 2.928 ± 0.063	3.848TyrLeu: 3.848 ± 0.08	0.8TyrMet: 0.8 ± 0.032	2.78TyrAsn: 2.78 ± 0.064	1.575TyrPro: 1.575 ± 0.048	1.446TyrGln: 1.446 ± 0.045	2.123TyrArg: 2.123 ± 0.052	2.792TyrSer: 2.792 ± 0.064	2.756TyrThr: 2.756 ± 0.08	2.326TyrVal: 2.326 ± 0.053	0.454TyrTrp: 0.454 ± 0.023	2.046TyrTyr: 2.046 ± 0.057	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.001XaaAla: 0.001 ± 0.001	0.002XaaCys: 0.002 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.008XaaGlu: 0.008 ± 0.003	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.005XaaIle: 0.005 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.002XaaMet: 0.002 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2995 proteins (945582 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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