Bacillus phage vB_BtS_BMBtp15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P7M0|A0A1B1P7M0_9CAUD Integrase OS=Bacillus phage vB_BtS_BMBtp15 OX=1868827 GN=BMBtpLA4_1 PE=4 SV=1
MM1 pKa = 7.33NKK3 pKa = 9.44VADD6 pKa = 3.95HH7 pKa = 7.19PIEE10 pKa = 4.99DD11 pKa = 4.46FYY13 pKa = 11.76GDD15 pKa = 4.34EE16 pKa = 4.12ILSGEE21 pKa = 4.27TYY23 pKa = 10.5FIFGEE28 pKa = 4.23HH29 pKa = 5.76VVLEE33 pKa = 4.54EE34 pKa = 3.83NLKK37 pKa = 10.86LYY39 pKa = 10.39LIQHH43 pKa = 5.6QNVEE47 pKa = 4.33CFRR50 pKa = 11.84AVV52 pKa = 2.85

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P7M4|A0A1B1P7M4_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp15 OX=1868827 GN=BMBtpLA4_16 PE=4 SV=1
MM1 pKa = 7.39NGVLSASKK9 pKa = 10.42LMKK12 pKa = 10.2ASQVRR17 pKa = 11.84KK18 pKa = 9.39QCAEE22 pKa = 3.74MRR24 pKa = 11.84NNPALLLAMEE34 pKa = 5.05LEE36 pKa = 4.72VKK38 pKa = 10.63RR39 pKa = 11.84NIYY42 pKa = 9.56EE43 pKa = 3.97INRR46 pKa = 11.84KK47 pKa = 9.19VSSS50 pKa = 3.45

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10714

45

1173

228.0

26.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.132 ± 0.469

0.859 ± 0.176

6.31 ± 0.282

8.68 ± 0.461

4.013 ± 0.201

5.955 ± 0.289

1.727 ± 0.184

7.271 ± 0.284

9.455 ± 0.341

7.812 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.229

5.628 ± 0.18

3.099 ± 0.224

3.715 ± 0.31

4.06 ± 0.288

5.236 ± 0.266

5.413 ± 0.233

6.394 ± 0.241

1.4 ± 0.211

4.237 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski