Amino acid dipepetide frequency for Bacillus phage vB_BtS_BMBtp15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.761AlaAla: 4.761 ± 1.226
0.373AlaCys: 0.373 ± 0.176
4.201AlaAsp: 4.201 ± 0.61
5.227AlaGlu: 5.227 ± 0.901
2.52AlaPhe: 2.52 ± 0.458
2.894AlaGly: 2.894 ± 0.462
1.027AlaHis: 1.027 ± 0.326
3.08AlaIle: 3.08 ± 0.515
6.907AlaLys: 6.907 ± 0.822
4.574AlaLeu: 4.574 ± 0.689
2.52AlaMet: 2.52 ± 0.458
2.894AlaAsn: 2.894 ± 0.481
1.4AlaPro: 1.4 ± 0.338
2.614AlaGln: 2.614 ± 0.516
1.867AlaArg: 1.867 ± 0.353
3.174AlaSer: 3.174 ± 0.524
3.174AlaThr: 3.174 ± 0.735
3.454AlaVal: 3.454 ± 0.757
1.494AlaTrp: 1.494 ± 0.674
3.174AlaTyr: 3.174 ± 0.33
0.0AlaXaa: 0.0 ± 0.0
Cys
0.373CysAla: 0.373 ± 0.205
0.373CysCys: 0.373 ± 0.191
0.467CysAsp: 0.467 ± 0.177
0.747CysGlu: 0.747 ± 0.219
0.56CysPhe: 0.56 ± 0.218
0.467CysGly: 0.467 ± 0.255
0.187CysHis: 0.187 ± 0.132
0.653CysIle: 0.653 ± 0.295
0.84CysLys: 0.84 ± 0.312
0.187CysLeu: 0.187 ± 0.122
0.28CysMet: 0.28 ± 0.137
0.28CysAsn: 0.28 ± 0.174
0.56CysPro: 0.56 ± 0.412
0.373CysGln: 0.373 ± 0.18
0.467CysArg: 0.467 ± 0.204
0.467CysSer: 0.467 ± 0.178
0.56CysThr: 0.56 ± 0.25
0.187CysVal: 0.187 ± 0.155
0.0CysTrp: 0.0 ± 0.0
0.56CysTyr: 0.56 ± 0.236
0.0CysXaa: 0.0 ± 0.0
Asp
2.8AspAla: 2.8 ± 0.566
0.373AspCys: 0.373 ± 0.187
3.827AspAsp: 3.827 ± 0.506
4.574AspGlu: 4.574 ± 0.573
3.267AspPhe: 3.267 ± 0.565
4.574AspGly: 4.574 ± 0.542
0.56AspHis: 0.56 ± 0.261
5.881AspIle: 5.881 ± 0.691
5.507AspLys: 5.507 ± 0.796
5.881AspLeu: 5.881 ± 0.88
1.96AspMet: 1.96 ± 0.473
2.614AspAsn: 2.614 ± 0.542
3.267AspPro: 3.267 ± 0.55
1.307AspGln: 1.307 ± 0.365
2.054AspArg: 2.054 ± 0.514
2.8AspSer: 2.8 ± 0.43
3.36AspThr: 3.36 ± 0.586
4.761AspVal: 4.761 ± 0.608
1.4AspTrp: 1.4 ± 0.446
2.334AspTyr: 2.334 ± 0.56
0.0AspXaa: 0.0 ± 0.0
Glu
5.601GluAla: 5.601 ± 1.063
0.747GluCys: 0.747 ± 0.39
4.574GluAsp: 4.574 ± 0.706
9.614GluGlu: 9.614 ± 1.352
2.894GluPhe: 2.894 ± 0.454
4.294GluGly: 4.294 ± 0.589
2.24GluHis: 2.24 ± 0.405
6.067GluIle: 6.067 ± 0.973
8.588GluLys: 8.588 ± 1.019
7.374GluLeu: 7.374 ± 0.743
2.8GluMet: 2.8 ± 0.554
5.134GluAsn: 5.134 ± 0.516
1.774GluPro: 1.774 ± 0.302
3.08GluGln: 3.08 ± 0.703
4.014GluArg: 4.014 ± 0.736
4.201GluSer: 4.201 ± 0.649
4.201GluThr: 4.201 ± 0.699
5.414GluVal: 5.414 ± 1.036
1.4GluTrp: 1.4 ± 0.556
2.8GluTyr: 2.8 ± 0.511
0.0GluXaa: 0.0 ± 0.0
Phe
2.52PheAla: 2.52 ± 0.462
0.373PheCys: 0.373 ± 0.179
2.52PheAsp: 2.52 ± 0.483
3.36PheGlu: 3.36 ± 0.839
0.84PhePhe: 0.84 ± 0.457
2.8PheGly: 2.8 ± 0.679
0.56PheHis: 0.56 ± 0.285
2.987PheIle: 2.987 ± 0.519
3.36PheLys: 3.36 ± 0.576
2.614PheLeu: 2.614 ± 0.568
0.933PheMet: 0.933 ± 0.284
2.147PheAsn: 2.147 ± 0.423
1.4PhePro: 1.4 ± 0.322
1.68PheGln: 1.68 ± 0.365
1.4PheArg: 1.4 ± 0.405
2.24PheSer: 2.24 ± 0.67
2.707PheThr: 2.707 ± 0.52
3.454PheVal: 3.454 ± 0.595
0.28PheTrp: 0.28 ± 0.186
1.96PheTyr: 1.96 ± 0.46
0.0PheXaa: 0.0 ± 0.0
Gly
4.014GlyAla: 4.014 ± 0.89
0.56GlyCys: 0.56 ± 0.243
3.267GlyAsp: 3.267 ± 0.61
5.227GlyGlu: 5.227 ± 0.483
2.52GlyPhe: 2.52 ± 0.394
3.827GlyGly: 3.827 ± 0.653
1.027GlyHis: 1.027 ± 0.313
3.92GlyIle: 3.92 ± 0.771
5.601GlyLys: 5.601 ± 0.735
4.574GlyLeu: 4.574 ± 0.646
1.587GlyMet: 1.587 ± 0.349
3.08GlyAsn: 3.08 ± 0.677
0.56GlyPro: 0.56 ± 0.211
2.52GlyGln: 2.52 ± 0.66
2.147GlyArg: 2.147 ± 0.484
3.36GlySer: 3.36 ± 0.536
2.8GlyThr: 2.8 ± 0.477
4.387GlyVal: 4.387 ± 0.584
0.933GlyTrp: 0.933 ± 0.201
3.64GlyTyr: 3.64 ± 0.734
0.0GlyXaa: 0.0 ± 0.0
His
0.84HisAla: 0.84 ± 0.267
0.187HisCys: 0.187 ± 0.12
0.653HisAsp: 0.653 ± 0.239
2.054HisGlu: 2.054 ± 0.482
0.933HisPhe: 0.933 ± 0.245
1.213HisGly: 1.213 ± 0.323
0.56HisHis: 0.56 ± 0.287
1.96HisIle: 1.96 ± 0.337
1.12HisLys: 1.12 ± 0.297
1.494HisLeu: 1.494 ± 0.461
0.187HisMet: 0.187 ± 0.13
0.84HisAsn: 0.84 ± 0.214
1.027HisPro: 1.027 ± 0.409
0.467HisGln: 0.467 ± 0.25
0.28HisArg: 0.28 ± 0.163
0.187HisSer: 0.187 ± 0.115
0.84HisThr: 0.84 ± 0.298
1.587HisVal: 1.587 ± 0.35
0.093HisTrp: 0.093 ± 0.087
0.747HisTyr: 0.747 ± 0.288
0.0HisXaa: 0.0 ± 0.0
Ile
4.667IleAla: 4.667 ± 0.705
0.933IleCys: 0.933 ± 0.303
4.107IleAsp: 4.107 ± 0.701
6.254IleGlu: 6.254 ± 0.882
2.24IlePhe: 2.24 ± 0.535
3.92IleGly: 3.92 ± 0.45
0.84IleHis: 0.84 ± 0.326
4.294IleIle: 4.294 ± 0.683
6.907IleLys: 6.907 ± 0.93
5.414IleLeu: 5.414 ± 0.838
2.52IleMet: 2.52 ± 0.521
4.107IleAsn: 4.107 ± 0.445
2.52IlePro: 2.52 ± 0.541
2.707IleGln: 2.707 ± 0.597
3.267IleArg: 3.267 ± 0.626
4.667IleSer: 4.667 ± 0.635
4.947IleThr: 4.947 ± 0.82
4.294IleVal: 4.294 ± 0.566
1.213IleTrp: 1.213 ± 0.377
2.894IleTyr: 2.894 ± 0.618
0.0IleXaa: 0.0 ± 0.0
Lys
5.694LysAla: 5.694 ± 0.784
0.653LysCys: 0.653 ± 0.292
5.414LysAsp: 5.414 ± 0.709
7.841LysGlu: 7.841 ± 0.996
3.547LysPhe: 3.547 ± 0.626
5.414LysGly: 5.414 ± 0.755
1.68LysHis: 1.68 ± 0.513
7.094LysIle: 7.094 ± 0.876
9.614LysLys: 9.614 ± 1.243
8.214LysLeu: 8.214 ± 1.119
2.52LysMet: 2.52 ± 0.486
6.067LysAsn: 6.067 ± 0.721
2.52LysPro: 2.52 ± 0.642
4.387LysGln: 4.387 ± 0.719
5.321LysArg: 5.321 ± 0.914
4.107LysSer: 4.107 ± 0.602
4.481LysThr: 4.481 ± 0.598
5.787LysVal: 5.787 ± 0.806
1.12LysTrp: 1.12 ± 0.259
2.987LysTyr: 2.987 ± 0.538
0.0LysXaa: 0.0 ± 0.0
Leu
4.854LeuAla: 4.854 ± 0.922
0.747LeuCys: 0.747 ± 0.274
5.974LeuAsp: 5.974 ± 0.701
6.534LeuGlu: 6.534 ± 0.796
3.267LeuPhe: 3.267 ± 0.649
4.761LeuGly: 4.761 ± 0.833
0.933LeuHis: 0.933 ± 0.258
6.161LeuIle: 6.161 ± 0.634
7.654LeuLys: 7.654 ± 1.108
6.254LeuLeu: 6.254 ± 0.912
1.68LeuMet: 1.68 ± 0.401
4.761LeuAsn: 4.761 ± 0.779
1.867LeuPro: 1.867 ± 0.361
2.987LeuGln: 2.987 ± 0.722
3.92LeuArg: 3.92 ± 0.509
4.761LeuSer: 4.761 ± 0.707
4.387LeuThr: 4.387 ± 0.58
3.36LeuVal: 3.36 ± 0.432
0.747LeuTrp: 0.747 ± 0.35
2.52LeuTyr: 2.52 ± 0.582
0.0LeuXaa: 0.0 ± 0.0
Met
1.4MetAla: 1.4 ± 0.396
0.093MetCys: 0.093 ± 0.098
2.147MetAsp: 2.147 ± 0.585
1.96MetGlu: 1.96 ± 0.373
0.56MetPhe: 0.56 ± 0.191
1.867MetGly: 1.867 ± 0.378
0.467MetHis: 0.467 ± 0.252
1.96MetIle: 1.96 ± 0.435
2.52MetLys: 2.52 ± 0.392
1.867MetLeu: 1.867 ± 0.465
0.653MetMet: 0.653 ± 0.317
2.427MetAsn: 2.427 ± 0.537
0.653MetPro: 0.653 ± 0.295
0.653MetGln: 0.653 ± 0.286
1.213MetArg: 1.213 ± 0.306
1.4MetSer: 1.4 ± 0.414
1.96MetThr: 1.96 ± 0.432
1.4MetVal: 1.4 ± 0.342
0.093MetTrp: 0.093 ± 0.111
0.747MetTyr: 0.747 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.36AsnAla: 3.36 ± 0.701
0.653AsnCys: 0.653 ± 0.303
4.107AsnAsp: 4.107 ± 0.502
4.667AsnGlu: 4.667 ± 0.591
2.054AsnPhe: 2.054 ± 0.386
5.321AsnGly: 5.321 ± 0.861
0.84AsnHis: 0.84 ± 0.241
4.574AsnIle: 4.574 ± 0.646
4.854AsnLys: 4.854 ± 0.669
4.574AsnLeu: 4.574 ± 0.633
1.12AsnMet: 1.12 ± 0.316
3.174AsnAsn: 3.174 ± 0.604
2.52AsnPro: 2.52 ± 0.495
1.68AsnGln: 1.68 ± 0.387
1.494AsnArg: 1.494 ± 0.556
2.334AsnSer: 2.334 ± 0.573
2.054AsnThr: 2.054 ± 0.439
3.36AsnVal: 3.36 ± 0.546
0.747AsnTrp: 0.747 ± 0.291
2.8AsnTyr: 2.8 ± 0.507
0.0AsnXaa: 0.0 ± 0.0
Pro
1.867ProAla: 1.867 ± 0.496
0.093ProCys: 0.093 ± 0.111
1.867ProAsp: 1.867 ± 0.454
2.24ProGlu: 2.24 ± 0.741
1.307ProPhe: 1.307 ± 0.364
1.774ProGly: 1.774 ± 0.362
0.84ProHis: 0.84 ± 0.229
2.52ProIle: 2.52 ± 0.411
3.08ProLys: 3.08 ± 0.827
3.267ProLeu: 3.267 ± 0.59
0.187ProMet: 0.187 ± 0.172
1.867ProAsn: 1.867 ± 0.63
0.84ProPro: 0.84 ± 0.257
0.747ProGln: 0.747 ± 0.272
1.027ProArg: 1.027 ± 0.28
1.307ProSer: 1.307 ± 0.323
2.147ProThr: 2.147 ± 0.518
2.614ProVal: 2.614 ± 0.71
0.187ProTrp: 0.187 ± 0.139
1.213ProTyr: 1.213 ± 0.444
0.0ProXaa: 0.0 ± 0.0
Gln
2.894GlnAla: 2.894 ± 0.618
0.373GlnCys: 0.373 ± 0.189
1.494GlnAsp: 1.494 ± 0.336
3.08GlnGlu: 3.08 ± 0.63
2.614GlnPhe: 2.614 ± 0.503
1.494GlnGly: 1.494 ± 0.356
0.653GlnHis: 0.653 ± 0.23
2.614GlnIle: 2.614 ± 0.376
3.547GlnLys: 3.547 ± 0.715
3.454GlnLeu: 3.454 ± 0.559
0.56GlnMet: 0.56 ± 0.189
1.494GlnAsn: 1.494 ± 0.427
1.12GlnPro: 1.12 ± 0.297
1.4GlnGln: 1.4 ± 0.426
1.774GlnArg: 1.774 ± 0.353
1.774GlnSer: 1.774 ± 0.317
1.68GlnThr: 1.68 ± 0.424
2.334GlnVal: 2.334 ± 0.542
0.653GlnTrp: 0.653 ± 0.255
2.147GlnTyr: 2.147 ± 0.492
0.0GlnXaa: 0.0 ± 0.0
Arg
2.8ArgAla: 2.8 ± 0.539
0.56ArgCys: 0.56 ± 0.264
2.427ArgAsp: 2.427 ± 0.39
3.454ArgGlu: 3.454 ± 0.612
1.494ArgPhe: 1.494 ± 0.391
2.147ArgGly: 2.147 ± 0.484
1.213ArgHis: 1.213 ± 0.4
2.707ArgIle: 2.707 ± 0.683
3.734ArgLys: 3.734 ± 0.625
2.147ArgLeu: 2.147 ± 0.533
1.96ArgMet: 1.96 ± 0.304
2.614ArgAsn: 2.614 ± 0.561
1.494ArgPro: 1.494 ± 0.408
1.4ArgGln: 1.4 ± 0.266
1.587ArgArg: 1.587 ± 0.414
1.96ArgSer: 1.96 ± 0.451
1.774ArgThr: 1.774 ± 0.336
2.987ArgVal: 2.987 ± 0.647
0.467ArgTrp: 0.467 ± 0.193
1.68ArgTyr: 1.68 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
3.454SerAla: 3.454 ± 0.586
0.093SerCys: 0.093 ± 0.092
3.547SerAsp: 3.547 ± 0.705
4.107SerGlu: 4.107 ± 0.855
2.24SerPhe: 2.24 ± 0.498
3.08SerGly: 3.08 ± 0.722
0.653SerHis: 0.653 ± 0.222
3.827SerIle: 3.827 ± 0.549
5.227SerLys: 5.227 ± 0.706
3.08SerLeu: 3.08 ± 0.529
1.12SerMet: 1.12 ± 0.363
2.894SerAsn: 2.894 ± 0.651
0.84SerPro: 0.84 ± 0.276
2.427SerGln: 2.427 ± 0.366
1.774SerArg: 1.774 ± 0.418
3.174SerSer: 3.174 ± 0.703
4.294SerThr: 4.294 ± 0.821
2.987SerVal: 2.987 ± 0.43
0.747SerTrp: 0.747 ± 0.283
2.8SerTyr: 2.8 ± 0.439
0.0SerXaa: 0.0 ± 0.0
Thr
4.014ThrAla: 4.014 ± 0.686
0.467ThrCys: 0.467 ± 0.205
4.481ThrAsp: 4.481 ± 0.494
3.827ThrGlu: 3.827 ± 0.553
1.96ThrPhe: 1.96 ± 0.34
3.547ThrGly: 3.547 ± 0.922
0.653ThrHis: 0.653 ± 0.251
3.547ThrIle: 3.547 ± 0.571
3.734ThrLys: 3.734 ± 0.569
4.854ThrLeu: 4.854 ± 0.664
1.027ThrMet: 1.027 ± 0.334
2.987ThrAsn: 2.987 ± 0.639
2.52ThrPro: 2.52 ± 0.529
2.427ThrGln: 2.427 ± 0.55
2.24ThrArg: 2.24 ± 0.361
2.987ThrSer: 2.987 ± 0.542
2.427ThrThr: 2.427 ± 0.394
3.64ThrVal: 3.64 ± 0.714
0.84ThrTrp: 0.84 ± 0.334
1.96ThrTyr: 1.96 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
2.8ValAla: 2.8 ± 0.439
0.28ValCys: 0.28 ± 0.169
3.92ValAsp: 3.92 ± 0.555
6.441ValGlu: 6.441 ± 1.034
2.894ValPhe: 2.894 ± 0.638
2.987ValGly: 2.987 ± 0.721
1.027ValHis: 1.027 ± 0.286
4.014ValIle: 4.014 ± 0.659
6.254ValLys: 6.254 ± 0.82
3.92ValLeu: 3.92 ± 0.536
1.587ValMet: 1.587 ± 0.551
3.92ValAsn: 3.92 ± 0.641
2.52ValPro: 2.52 ± 0.488
2.614ValGln: 2.614 ± 0.549
2.147ValArg: 2.147 ± 0.451
4.201ValSer: 4.201 ± 0.468
3.734ValThr: 3.734 ± 0.591
4.947ValVal: 4.947 ± 0.701
0.653ValTrp: 0.653 ± 0.2
3.08ValTyr: 3.08 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
0.653TrpAla: 0.653 ± 0.271
0.0TrpCys: 0.0 ± 0.0
1.12TrpAsp: 1.12 ± 0.375
1.213TrpGlu: 1.213 ± 0.286
0.653TrpPhe: 0.653 ± 0.246
0.933TrpGly: 0.933 ± 0.321
0.56TrpHis: 0.56 ± 0.321
1.213TrpIle: 1.213 ± 0.269
1.4TrpLys: 1.4 ± 0.432
1.213TrpLeu: 1.213 ± 0.371
0.187TrpMet: 0.187 ± 0.149
0.373TrpAsn: 0.373 ± 0.254
0.0TrpPro: 0.0 ± 0.0
0.373TrpGln: 0.373 ± 0.148
0.84TrpArg: 0.84 ± 0.306
1.12TrpSer: 1.12 ± 0.338
0.747TrpThr: 0.747 ± 0.282
0.467TrpVal: 0.467 ± 0.245
0.373TrpTrp: 0.373 ± 0.302
0.56TrpTyr: 0.56 ± 0.279
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.96TyrAla: 1.96 ± 0.491
0.653TyrCys: 0.653 ± 0.269
2.987TyrAsp: 2.987 ± 0.725
4.387TyrGlu: 4.387 ± 0.851
1.774TyrPhe: 1.774 ± 0.3
2.24TyrGly: 2.24 ± 0.421
0.747TyrHis: 0.747 ± 0.252
3.64TyrIle: 3.64 ± 0.668
4.107TyrLys: 4.107 ± 0.878
3.174TyrLeu: 3.174 ± 0.68
0.56TyrMet: 0.56 ± 0.166
2.614TyrAsn: 2.614 ± 0.484
1.587TyrPro: 1.587 ± 0.383
1.307TyrGln: 1.307 ± 0.282
1.774TyrArg: 1.774 ± 0.38
2.147TyrSer: 2.147 ± 0.489
1.867TyrThr: 1.867 ± 0.359
2.52TyrVal: 2.52 ± 0.397
0.56TyrTrp: 0.56 ± 0.237
1.774TyrTyr: 1.774 ± 0.588
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (10714 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski