Alteromonadales bacterium alter-6D02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; unclassified Alteromonadales

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0WML2|A0A2N0WML2_9GAMM Chain length determinant family protein OS=Alteromonadales bacterium alter-6D02 OX=2058087 GN=CW748_14130 PE=4 SV=1
MM1 pKa = 7.05NVAMNTPPTPGAQTPAQEE19 pKa = 4.41VIKK22 pKa = 10.27VTGQTPLTNDD32 pKa = 2.97QQGPYY37 pKa = 10.3LLGVAQKK44 pKa = 8.56ITAEE48 pKa = 4.12EE49 pKa = 4.21IEE51 pKa = 4.31RR52 pKa = 11.84SNAKK56 pKa = 8.41TLTQQLKK63 pKa = 8.29NTLVSVNINDD73 pKa = 3.59VQNNPFQPDD82 pKa = 3.13VQYY85 pKa = 11.16RR86 pKa = 11.84GFTASPLLGLPQGISVYY103 pKa = 10.87LDD105 pKa = 3.38GIRR108 pKa = 11.84FNEE111 pKa = 4.1PFGDD115 pKa = 3.78TVNWDD120 pKa = 4.91LIALTAIDD128 pKa = 4.72NVTLYY133 pKa = 10.75SGSNPVFGQNTLGGALALSVKK154 pKa = 10.71DD155 pKa = 3.22GFTYY159 pKa = 10.51DD160 pKa = 3.31GHH162 pKa = 6.28EE163 pKa = 4.57LSAQAGSFGEE173 pKa = 4.18KK174 pKa = 9.63QLNVQSGGTSGNWGYY189 pKa = 11.18YY190 pKa = 10.49LNLNHH195 pKa = 6.39YY196 pKa = 9.86EE197 pKa = 3.87EE198 pKa = 5.67DD199 pKa = 3.21GWRR202 pKa = 11.84DD203 pKa = 3.43GSPSEE208 pKa = 4.23VKK210 pKa = 10.41QYY212 pKa = 10.95LANLSYY218 pKa = 9.75KK219 pKa = 10.06TDD221 pKa = 4.07DD222 pKa = 3.6LTADD226 pKa = 4.43LLFATNNNTMIGNGTVPIEE245 pKa = 4.05LLEE248 pKa = 4.25QEE250 pKa = 4.7SSTAIYY256 pKa = 7.54TQPDD260 pKa = 3.38QTKK263 pKa = 9.68TNLNMLGLNITAALTEE279 pKa = 4.47DD280 pKa = 3.2SSLAVNAYY288 pKa = 8.37YY289 pKa = 10.49RR290 pKa = 11.84KK291 pKa = 10.31NEE293 pKa = 3.65ISSINGDD300 pKa = 3.36DD301 pKa = 3.65SDD303 pKa = 4.4YY304 pKa = 11.64DD305 pKa = 3.53EE306 pKa = 6.24CEE308 pKa = 3.89FGTAVTLCEE317 pKa = 5.32GEE319 pKa = 5.4DD320 pKa = 4.35DD321 pKa = 6.08DD322 pKa = 7.0DD323 pKa = 6.76DD324 pKa = 5.51EE325 pKa = 6.44IDD327 pKa = 4.48GIEE330 pKa = 4.15SHH332 pKa = 7.04GDD334 pKa = 3.26DD335 pKa = 4.33DD336 pKa = 6.32DD337 pKa = 4.51EE338 pKa = 6.59GEE340 pKa = 4.39EE341 pKa = 3.99IEE343 pKa = 4.29PVHH346 pKa = 5.71FVGYY350 pKa = 10.81NEE352 pKa = 4.11GQYY355 pKa = 11.08LSDD358 pKa = 4.41IMPALDD364 pKa = 4.94ADD366 pKa = 5.45DD367 pKa = 5.22IDD369 pKa = 3.87GTYY372 pKa = 8.99NTGATDD378 pKa = 3.48NEE380 pKa = 4.68SFGFSAQLVTQWGNGHH396 pKa = 5.99EE397 pKa = 4.34LIIGGGADD405 pKa = 3.17KK406 pKa = 11.26ADD408 pKa = 3.08ISFASDD414 pKa = 3.1TQFAILHH421 pKa = 6.48NDD423 pKa = 3.01TAEE426 pKa = 4.72DD427 pKa = 3.89DD428 pKa = 4.3RR429 pKa = 11.84SVTPIPGLFDD439 pKa = 3.27QEE441 pKa = 4.46STVRR445 pKa = 11.84LDD447 pKa = 3.6TEE449 pKa = 4.02TTQYY453 pKa = 11.42YY454 pKa = 9.84FYY456 pKa = 10.7LANRR460 pKa = 11.84FKK462 pKa = 10.86LASDD466 pKa = 3.85LHH468 pKa = 8.15LDD470 pKa = 3.15IAGRR474 pKa = 11.84YY475 pKa = 8.0HH476 pKa = 8.01DD477 pKa = 5.91SNILMNDD484 pKa = 4.39LIDD487 pKa = 4.86DD488 pKa = 4.54GEE490 pKa = 4.9GSLDD494 pKa = 3.57GDD496 pKa = 3.69HH497 pKa = 7.4DD498 pKa = 4.63FSRR501 pKa = 11.84FNPAIGLSYY510 pKa = 10.45QISPQLTAKK519 pKa = 10.63LSYY522 pKa = 10.38SEE524 pKa = 4.61SSRR527 pKa = 11.84NPSPAEE533 pKa = 3.73LSCADD538 pKa = 4.11EE539 pKa = 5.82DD540 pKa = 5.48DD541 pKa = 4.21PCKK544 pKa = 10.66LPNGFVSDD552 pKa = 4.38PPLKK556 pKa = 10.01QVVVNTVEE564 pKa = 4.64LSLLGKK570 pKa = 10.04DD571 pKa = 4.18DD572 pKa = 3.77NLNYY576 pKa = 10.67GVTFFSTQSQDD587 pKa = 4.97DD588 pKa = 4.39IIFQQAGEE596 pKa = 4.29HH597 pKa = 6.43PARR600 pKa = 11.84GYY602 pKa = 10.03FVNIDD607 pKa = 3.17EE608 pKa = 4.5TQRR611 pKa = 11.84RR612 pKa = 11.84GVEE615 pKa = 3.87LSASYY620 pKa = 10.25RR621 pKa = 11.84GEE623 pKa = 4.07SFDD626 pKa = 3.21ITSSYY631 pKa = 11.36NYY633 pKa = 10.42LDD635 pKa = 3.62ATFEE639 pKa = 4.54SPFVSFSPVNPLGPNRR655 pKa = 11.84QVSPGDD661 pKa = 3.8TIPGQPQHH669 pKa = 5.9QIKK672 pKa = 10.95LIGQYY677 pKa = 9.76QATEE681 pKa = 4.03SFDD684 pKa = 3.26IGSEE688 pKa = 3.8LSYY691 pKa = 11.2ASEE694 pKa = 4.02SYY696 pKa = 10.98YY697 pKa = 10.92RR698 pKa = 11.84GDD700 pKa = 3.39EE701 pKa = 4.12ANEE704 pKa = 3.7NQQIGSHH711 pKa = 4.7TVVNLFANYY720 pKa = 10.17QITQQLALEE729 pKa = 4.26LRR731 pKa = 11.84VDD733 pKa = 3.77NVFDD737 pKa = 3.55RR738 pKa = 11.84QYY740 pKa = 10.37FTFGAYY746 pKa = 10.04GEE748 pKa = 4.17ADD750 pKa = 3.05EE751 pKa = 5.0VLEE754 pKa = 4.08NVYY757 pKa = 10.63PDD759 pKa = 3.35IEE761 pKa = 4.38DD762 pKa = 4.08PYY764 pKa = 11.18FVGPAKK770 pKa = 9.99PRR772 pKa = 11.84SVSVEE777 pKa = 3.63LSYY780 pKa = 11.19QFF782 pKa = 4.51

Molecular weight: 85.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0WMW9|A0A2N0WMW9_9GAMM Cysteine--tRNA ligase OS=Alteromonadales bacterium alter-6D02 OX=2058087 GN=cysS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.35GFRR19 pKa = 11.84ARR21 pKa = 11.84SATKK25 pKa = 10.08GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.49GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3415

0

3415

1160949

23

3442

340.0

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.321 ± 0.044

0.988 ± 0.013

5.558 ± 0.036

5.944 ± 0.041

4.187 ± 0.029

6.374 ± 0.04

2.304 ± 0.021

6.707 ± 0.034

5.446 ± 0.037

10.57 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.019

4.62 ± 0.032

3.666 ± 0.029

5.282 ± 0.044

4.18 ± 0.033

7.003 ± 0.034

5.515 ± 0.042

6.807 ± 0.036

1.112 ± 0.014

3.083 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski