Aliicoccus persicus
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1983 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A662Z3Y1|A0A662Z3Y1_9STAP Uncharacterized protein OS=Aliicoccus persicus OX=930138 GN=SAMN05192557_0616 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.67 KK3 pKa = 9.42 MIKK6 pKa = 10.25 LFTVSMLIGTTVAYY20 pKa = 10.4 FGTTTHH26 pKa = 6.5 AQDD29 pKa = 3.34 EE30 pKa = 4.66 ADD32 pKa = 3.51 TVEE35 pKa = 3.91 ISMTIGNDD43 pKa = 2.89 IDD45 pKa = 4.16 SLDD48 pKa = 3.95 PFLAQATLTDD58 pKa = 3.69 SVIYY62 pKa = 10.6 NIFEE66 pKa = 4.28 GLLDD70 pKa = 4.18 AEE72 pKa = 4.82 TDD74 pKa = 3.73 GSLVPHH80 pKa = 7.26 LAHH83 pKa = 7.48 DD84 pKa = 4.16 YY85 pKa = 10.33 EE86 pKa = 5.59 ISDD89 pKa = 4.58 DD90 pKa = 3.68 ALTYY94 pKa = 10.59 TFFLEE99 pKa = 4.39 EE100 pKa = 4.32 GVTFHH105 pKa = 7.57 DD106 pKa = 4.92 GSDD109 pKa = 3.69 FTAEE113 pKa = 4.07 DD114 pKa = 3.31 VVYY117 pKa = 9.27 TYY119 pKa = 11.52 SKK121 pKa = 10.82 LAGLDD126 pKa = 3.77 GEE128 pKa = 5.4 DD129 pKa = 3.59 ALSDD133 pKa = 2.95 KK134 pKa = 10.77 WEE136 pKa = 4.08 VVEE139 pKa = 5.35 SVEE142 pKa = 4.29 ATDD145 pKa = 4.79 DD146 pKa = 3.73 YY147 pKa = 11.05 TVEE150 pKa = 4.31 VTLSNPDD157 pKa = 3.11 SGFLARR163 pKa = 11.84 TIRR166 pKa = 11.84 AIVPADD172 pKa = 3.81 YY173 pKa = 10.54 DD174 pKa = 3.68 DD175 pKa = 5.13 HH176 pKa = 6.33 ATNPVGTGPYY186 pKa = 9.34 TFVEE190 pKa = 4.15 HH191 pKa = 7.36 SIDD194 pKa = 4.11 QRR196 pKa = 11.84 VTLEE200 pKa = 3.98 KK201 pKa = 10.46 FDD203 pKa = 6.78 DD204 pKa = 4.0 YY205 pKa = 11.61 FQGNDD210 pKa = 3.03 FDD212 pKa = 4.79 VDD214 pKa = 3.94 VVNFDD219 pKa = 3.62 VMPDD223 pKa = 3.32 EE224 pKa = 4.46 EE225 pKa = 4.44 TALLAFQAGEE235 pKa = 4.0 VDD237 pKa = 5.33 FITAVSDD244 pKa = 3.6 QNLSRR249 pKa = 11.84 VPEE252 pKa = 4.34 DD253 pKa = 3.47 ANVVSGPLNMVMMLAMNHH271 pKa = 5.2 EE272 pKa = 4.53 VEE274 pKa = 4.63 PLDD277 pKa = 3.85 SVEE280 pKa = 4.34 VRR282 pKa = 11.84 QAMNMVVDD290 pKa = 3.97 KK291 pKa = 11.59 QEE293 pKa = 4.48 VIDD296 pKa = 4.4 VAIDD300 pKa = 3.67 GNGDD304 pKa = 3.67 ALHH307 pKa = 6.44 TFMSPAMDD315 pKa = 4.35 FYY317 pKa = 11.49 FNEE320 pKa = 4.23 EE321 pKa = 3.85 LVDD324 pKa = 3.98 YY325 pKa = 7.96 YY326 pKa = 11.14 TVDD329 pKa = 3.15 VEE331 pKa = 4.62 TAQSLMAEE339 pKa = 4.43 AGYY342 pKa = 11.03 EE343 pKa = 3.99 DD344 pKa = 4.46 GFSIEE349 pKa = 4.16 MKK351 pKa = 10.45 VPNNAQLYY359 pKa = 9.01 IDD361 pKa = 4.61 AAQVLQAQLALINIDD376 pKa = 3.41 LTLDD380 pKa = 3.62 VIEE383 pKa = 4.61 FSTWLEE389 pKa = 3.64 DD390 pKa = 3.6 VYY392 pKa = 11.4 NEE394 pKa = 4.61 RR395 pKa = 11.84 EE396 pKa = 4.07 FEE398 pKa = 4.1 STIIGFTGKK407 pKa = 10.24 LDD409 pKa = 4.22 PYY411 pKa = 11.1 DD412 pKa = 3.82 VMIRR416 pKa = 11.84 MEE418 pKa = 3.94 TGYY421 pKa = 10.89 AYY423 pKa = 11.29 NFMNVDD429 pKa = 2.94 IEE431 pKa = 4.81 GYY433 pKa = 10.5 NEE435 pKa = 3.86 ALEE438 pKa = 4.39 AAISAPSPEE447 pKa = 4.34 EE448 pKa = 3.64 SATLYY453 pKa = 10.91 KK454 pKa = 10.18 EE455 pKa = 4.14 AQEE458 pKa = 4.59 LLTEE462 pKa = 3.92 NAAAVFLMDD471 pKa = 4.32 PARR474 pKa = 11.84 NSVMRR479 pKa = 11.84 SGISGFEE486 pKa = 3.62 MYY488 pKa = 9.97 PYY490 pKa = 10.43 EE491 pKa = 4.92 RR492 pKa = 11.84 YY493 pKa = 9.26 VVKK496 pKa = 10.81 DD497 pKa = 3.41 LVVEE501 pKa = 4.45
Molecular weight: 55.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.605
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.834
Patrickios 1.151
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A662Z256|A0A662Z256_9STAP Transcriptional regulator LacI family OS=Aliicoccus persicus OX=930138 GN=SAMN05192557_0664 PE=4 SV=1
MM1 pKa = 7.49 VKK3 pKa = 9.1 RR4 pKa = 11.84 TYY6 pKa = 10.35 QPNKK10 pKa = 8.16 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.87 VHH17 pKa = 5.77 GFRR20 pKa = 11.84 KK21 pKa = 10.02 RR22 pKa = 11.84 MSTKK26 pKa = 10.16 NGRR29 pKa = 11.84 HH30 pKa = 3.62 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 8.94 VLSAA45 pKa = 4.11
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1983
0
1983
597384
30
2252
301.3
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.311 ± 0.066
0.493 ± 0.015
6.541 ± 0.069
8.158 ± 0.074
4.602 ± 0.047
6.271 ± 0.048
2.218 ± 0.029
8.152 ± 0.057
6.022 ± 0.06
9.216 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.9 ± 0.026
5.012 ± 0.042
3.182 ± 0.026
3.207 ± 0.029
4.126 ± 0.041
6.021 ± 0.037
5.639 ± 0.043
7.351 ± 0.044
0.732 ± 0.017
3.847 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here