Oleomonas sp. K1W22B-8
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5024 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418WDE3|A0A418WDE3_9PROT DNA topoisomerase 4 subunit A OS=Oleomonas sp. K1W22B-8 OX=2320859 GN=parC PE=3 SV=1
MM1 pKa = 7.63 PAFAEE6 pKa = 4.39 TFDD9 pKa = 5.02 LADD12 pKa = 4.33 LDD14 pKa = 4.44 GEE16 pKa = 4.5 NGFHH20 pKa = 6.52 LVSEE24 pKa = 4.43 AGSYY28 pKa = 10.19 NFGYY32 pKa = 10.48 SIATLGDD39 pKa = 3.49 VNGDD43 pKa = 3.61 GFDD46 pKa = 5.12 DD47 pKa = 3.61 IAVNSFDD54 pKa = 4.81 GPTILLGGQGPFAPSTTVSNEE75 pKa = 3.65 FPFEE79 pKa = 4.03 TRR81 pKa = 11.84 TATLPDD87 pKa = 4.53 DD88 pKa = 3.68 LTEE91 pKa = 4.16 YY92 pKa = 10.44 EE93 pKa = 4.23 SWIVGIGDD101 pKa = 3.5 VNGDD105 pKa = 3.33 GFADD109 pKa = 4.07 FDD111 pKa = 3.61 HH112 pKa = 6.8 HH113 pKa = 7.21 AFYY116 pKa = 9.18 ATEE119 pKa = 4.02 TYY121 pKa = 10.64 YY122 pKa = 10.61 EE123 pKa = 4.13 YY124 pKa = 10.46 EE125 pKa = 4.47 YY126 pKa = 11.1 GDD128 pKa = 3.31 SWTEE132 pKa = 3.96 IFFHH136 pKa = 7.48 DD137 pKa = 4.4 YY138 pKa = 11.31 GRR140 pKa = 11.84 IQYY143 pKa = 10.26 GGGSAATTGKK153 pKa = 10.44 RR154 pKa = 11.84 FGDD157 pKa = 3.56 RR158 pKa = 11.84 GLEE161 pKa = 4.06 DD162 pKa = 4.68 VDD164 pKa = 4.47 PSWLVPLGDD173 pKa = 3.75 VNGDD177 pKa = 3.42 GFDD180 pKa = 3.74 DD181 pKa = 3.89 VVVYY185 pKa = 10.66 VQDD188 pKa = 3.82 GSHH191 pKa = 6.71 PKK193 pKa = 10.13 VVFGSATGLPDD204 pKa = 3.29 NVNRR208 pKa = 11.84 GSAPSLAFTGASPFEE223 pKa = 4.29 INGAGDD229 pKa = 3.52 VNGDD233 pKa = 4.0 GITDD237 pKa = 3.69 VVVGVSDD244 pKa = 3.52 SSGVDD249 pKa = 2.95 HH250 pKa = 6.11 YY251 pKa = 11.63 VVYY254 pKa = 10.61 GGAGLAPGFFDD265 pKa = 4.64 LTSLDD270 pKa = 3.39 GTNGFKK276 pKa = 10.26 LTDD279 pKa = 3.41 ARR281 pKa = 11.84 TVGTVGDD288 pKa = 3.79 VDD290 pKa = 4.99 GDD292 pKa = 4.01 GLDD295 pKa = 4.0 DD296 pKa = 4.78 LVVSMSGRR304 pKa = 11.84 TSSDD308 pKa = 2.7 PYY310 pKa = 8.83 YY311 pKa = 10.84 VVYY314 pKa = 10.63 GRR316 pKa = 11.84 AAGPAAIDD324 pKa = 3.54 ASAVDD329 pKa = 4.37 GSDD332 pKa = 2.85 GFQIVNAGTRR342 pKa = 11.84 WVGRR346 pKa = 11.84 AGDD349 pKa = 3.77 INADD353 pKa = 3.63 GLDD356 pKa = 4.16 DD357 pKa = 4.54 FLIGNDD363 pKa = 3.26 YY364 pKa = 11.32 VVFGRR369 pKa = 11.84 ADD371 pKa = 3.21 VGTGGLDD378 pKa = 3.19 ATSVDD383 pKa = 3.97 GDD385 pKa = 3.5 NGFQIVATGIDD396 pKa = 3.87 TTSSWGGLIVQAAGDD411 pKa = 3.93 VNGDD415 pKa = 3.57 GVDD418 pKa = 3.8 DD419 pKa = 4.56 LVAGTRR425 pKa = 11.84 LEE427 pKa = 4.17 NSVHH431 pKa = 6.23 VILGHH436 pKa = 6.78 AASAVNWVGTSARR449 pKa = 11.84 EE450 pKa = 3.9 NHH452 pKa = 6.48 GGSPEE457 pKa = 3.97 NDD459 pKa = 3.45 VLNGAGGNDD468 pKa = 4.13 YY469 pKa = 11.13 LRR471 pKa = 11.84 GWAVTTCC478 pKa = 3.65
Molecular weight: 49.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.452
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.139
Thurlkill 3.516
EMBOSS 3.681
Sillero 3.834
Patrickios 1.38
IPC_peptide 3.706
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A418WTK9|A0A418WTK9_9PROT Uncharacterized protein OS=Oleomonas sp. K1W22B-8 OX=2320859 GN=D3874_01865 PE=4 SV=1
MM1 pKa = 7.93 PSPSSRR7 pKa = 11.84 KK8 pKa = 6.83 PWRR11 pKa = 11.84 WRR13 pKa = 11.84 LSWRR17 pKa = 11.84 KK18 pKa = 9.38 SPPRR22 pKa = 11.84 KK23 pKa = 9.29 PPPRR27 pKa = 11.84 PSRR30 pKa = 11.84 RR31 pKa = 11.84 PLTAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 SPVVNRR45 pKa = 11.84 RR46 pKa = 11.84 PKK48 pKa = 9.9 VVRR51 pKa = 11.84 PPPMPGAANRR61 pKa = 11.84 FSVANRR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 SNGGPRR75 pKa = 11.84 WRR77 pKa = 11.84 SVGRR81 pKa = 11.84 RR82 pKa = 11.84 GPAANSNRR90 pKa = 11.84 FNRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 GLQAGDD102 pKa = 3.69 PWVSPAGRR110 pKa = 11.84 CRR112 pKa = 11.84 HH113 pKa = 5.21 KK114 pKa = 11.07 SMRR117 pKa = 11.84 KK118 pKa = 9.49 DD119 pKa = 3.76 FPRR122 pKa = 5.34
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.676
Toseland 12.837
ProMoST 13.334
Dawson 12.837
Bjellqvist 12.837
Wikipedia 13.305
Rodwell 12.413
Grimsley 12.866
Solomon 13.334
Lehninger 13.232
Nozaki 12.837
DTASelect 12.837
Thurlkill 12.837
EMBOSS 13.334
Sillero 12.837
Patrickios 12.135
IPC_peptide 13.334
IPC2_peptide 12.325
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5024
0
5024
1531126
26
3535
304.8
32.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.388 ± 0.057
0.821 ± 0.011
5.925 ± 0.039
5.126 ± 0.032
3.647 ± 0.023
9.284 ± 0.055
1.984 ± 0.02
4.948 ± 0.024
2.906 ± 0.03
10.369 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.26 ± 0.02
2.421 ± 0.025
5.437 ± 0.031
2.975 ± 0.017
7.206 ± 0.04
4.836 ± 0.028
5.356 ± 0.028
7.56 ± 0.03
1.357 ± 0.015
2.194 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here