Rhizobium smilacinae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5526 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C4XJ63|A0A5C4XJ63_9RHIZ Inositol-1-monophosphatase OS=Rhizobium smilacinae OX=1395944 GN=FHP24_15605 PE=3 SV=1
MM1 pKa = 7.35TNSISNALGVALYY14 pKa = 10.71YY15 pKa = 10.76SGSSKK20 pKa = 10.9AWFSATGSGPIQYY33 pKa = 7.86GTAGNDD39 pKa = 3.8SMWGDD44 pKa = 3.38SSVNVTMTGGTGDD57 pKa = 4.7DD58 pKa = 2.99IYY60 pKa = 11.77YY61 pKa = 10.53LYY63 pKa = 10.76SDD65 pKa = 3.96KK66 pKa = 11.22NRR68 pKa = 11.84AEE70 pKa = 4.16EE71 pKa = 4.07KK72 pKa = 10.52AGEE75 pKa = 4.3GVDD78 pKa = 5.46TIDD81 pKa = 2.52TWMSYY86 pKa = 7.26TLPEE90 pKa = 3.96NFEE93 pKa = 4.28NLRR96 pKa = 11.84VTGDD100 pKa = 2.87NRR102 pKa = 11.84YY103 pKa = 10.34AFGNSADD110 pKa = 4.02NIITGSSSRR119 pKa = 11.84QTIDD123 pKa = 2.75GGAGNDD129 pKa = 3.49VLIGGGGADD138 pKa = 3.41TFIISKK144 pKa = 11.04GNGTDD149 pKa = 4.64LIADD153 pKa = 4.73FSSDD157 pKa = 3.78DD158 pKa = 3.98RR159 pKa = 11.84IRR161 pKa = 11.84LDD163 pKa = 3.67GYY165 pKa = 11.04GVTSFDD171 pKa = 3.53QLISQSSQQGNDD183 pKa = 2.59LWLNLGNGEE192 pKa = 4.46SVVLAGTTADD202 pKa = 5.27DD203 pKa = 4.19LSADD207 pKa = 3.67QFEE210 pKa = 5.15LNLDD214 pKa = 3.77RR215 pKa = 11.84SSLTLSFNEE224 pKa = 4.62DD225 pKa = 3.54FNSLSLFDD233 pKa = 4.41GQDD236 pKa = 3.8GVWEE240 pKa = 4.19AKK242 pKa = 10.07YY243 pKa = 9.14WWAPEE248 pKa = 4.0KK249 pKa = 10.69GASLHH254 pKa = 6.36TNGEE258 pKa = 4.26QQWYY262 pKa = 9.67VNPAYY267 pKa = 10.53DD268 pKa = 4.03PTASANPFSVTDD280 pKa = 3.93GVLTIRR286 pKa = 11.84AEE288 pKa = 4.03QTPDD292 pKa = 3.24ALSGEE297 pKa = 4.53VEE299 pKa = 4.55GYY301 pKa = 10.79DD302 pKa = 3.66YY303 pKa = 11.18TSGMLTTHH311 pKa = 7.26ASFAQTYY318 pKa = 9.42GYY320 pKa = 10.77FEE322 pKa = 4.72IRR324 pKa = 11.84ADD326 pKa = 3.68MPDD329 pKa = 3.47EE330 pKa = 4.14QGAWPAFWLLPEE342 pKa = 5.2DD343 pKa = 4.82GSWPPEE349 pKa = 3.64LDD351 pKa = 3.61VIEE354 pKa = 5.03MRR356 pKa = 11.84GQDD359 pKa = 4.05PNTLIMSAHH368 pKa = 6.06SNEE371 pKa = 4.43TGQQTSVIKK380 pKa = 10.72NVAVTDD386 pKa = 3.61TEE388 pKa = 4.62GFHH391 pKa = 6.81TYY393 pKa = 10.64GLLWDD398 pKa = 4.42EE399 pKa = 5.7DD400 pKa = 4.33HH401 pKa = 6.44ITWYY405 pKa = 10.65FDD407 pKa = 3.53DD408 pKa = 4.01VAVAQTDD415 pKa = 4.06TPADD419 pKa = 3.63MHH421 pKa = 7.35DD422 pKa = 3.79PMYY425 pKa = 10.22MIVNLAVGGMAGTPSDD441 pKa = 4.16EE442 pKa = 4.71LPDD445 pKa = 4.05GSEE448 pKa = 3.86MNIDD452 pKa = 3.97YY453 pKa = 10.66IRR455 pKa = 11.84AYY457 pKa = 10.96SLDD460 pKa = 3.9DD461 pKa = 4.07LEE463 pKa = 5.54ASNTASAVQPSEE475 pKa = 3.74WLLL478 pKa = 3.6

Molecular weight: 51.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C4XSC8|A0A5C4XSC8_9RHIZ Phosphonate ABC transporter substrate-binding protein OS=Rhizobium smilacinae OX=1395944 GN=FHP24_08570 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.22GGRR28 pKa = 11.84KK29 pKa = 9.06VLSARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ASLSAA44 pKa = 3.92

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5526

0

5526

1739106

24

2820

314.7

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.527 ± 0.04

0.765 ± 0.011

5.724 ± 0.027

5.723 ± 0.031

3.964 ± 0.026

8.302 ± 0.03

1.971 ± 0.017

5.927 ± 0.024

3.826 ± 0.028

9.945 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.015

2.899 ± 0.015

4.777 ± 0.026

3.173 ± 0.02

6.422 ± 0.029

6.034 ± 0.027

5.472 ± 0.03

7.334 ± 0.026

1.257 ± 0.013

2.342 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski