Haliea sp.
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5874 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519IFT1|A0A519IFT1_9GAMM VOC family protein OS=Haliea sp. OX=1932666 GN=EOO54_21010 PE=4 SV=1
MM1 pKa = 8.0 ALMCPRR7 pKa = 11.84 CGADD11 pKa = 3.04 NGDD14 pKa = 3.44 TARR17 pKa = 11.84 FCKK20 pKa = 10.57 SCGEE24 pKa = 4.21 GLQAAAPVVQSSGTAPTEE42 pKa = 3.7 PSAAPVCSSCGAVNSLGARR61 pKa = 11.84 FCKK64 pKa = 10.52 SCGTLLPAAASTAAAAAVVTPLPVTSPLQASGAAAFSPASPSLFTEE110 pKa = 5.1 PPADD114 pKa = 3.7 PVSTALPSPVAADD127 pKa = 3.89 TSHH130 pKa = 6.3 SPLWILLIAALLVLALGGGGYY151 pKa = 9.68 WYY153 pKa = 10.21 VQNRR157 pKa = 11.84 GMPAFGALMGDD168 pKa = 3.69 SDD170 pKa = 4.38 PAAGSSDD177 pKa = 3.07 AAEE180 pKa = 4.05 VEE182 pKa = 4.26 KK183 pKa = 11.01 QEE185 pKa = 4.89 EE186 pKa = 4.67 VLTDD190 pKa = 3.26 TAEE193 pKa = 4.21 AADD196 pKa = 4.01 EE197 pKa = 4.09 EE198 pKa = 5.3 VITEE202 pKa = 4.22 VEE204 pKa = 3.62 VDD206 pKa = 3.53 AAADD210 pKa = 3.76 TAADD214 pKa = 3.69 AAADD218 pKa = 3.52 AAAPAEE224 pKa = 4.57 TQASPAA230 pKa = 3.69
Molecular weight: 22.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.884
Patrickios 0.655
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A519IE07|A0A519IE07_9GAMM AAA family ATPase (Fragment) OS=Haliea sp. OX=1932666 GN=EOO54_21355 PE=4 SV=1
MM1 pKa = 7.52 NLRR4 pKa = 11.84 HH5 pKa = 5.81 MEE7 pKa = 3.9 VFRR10 pKa = 11.84 AVMLTGGVVGAAEE23 pKa = 4.88 LLHH26 pKa = 6.64 ISQPAVSKK34 pKa = 10.73 ILAQAQRR41 pKa = 11.84 QLGFALFVRR50 pKa = 11.84 IKK52 pKa = 10.81 GRR54 pKa = 11.84 LVPTAEE60 pKa = 4.15 GQALHH65 pKa = 7.01 AEE67 pKa = 4.56 VEE69 pKa = 4.58 TLWRR73 pKa = 11.84 SVQRR77 pKa = 11.84 VQATPRR83 pKa = 11.84 SGTLTLAVSASLAPYY98 pKa = 9.74 LVPRR102 pKa = 11.84 TLATLARR109 pKa = 11.84 RR110 pKa = 11.84 FPGLQTRR117 pKa = 11.84 MEE119 pKa = 4.17 ILVTPIMVDD128 pKa = 3.0 ALLDD132 pKa = 3.5 RR133 pKa = 11.84 TADD136 pKa = 3.05 IGVAILPNDD145 pKa = 3.92 HH146 pKa = 7.28 PNLVQVSRR154 pKa = 11.84 HH155 pKa = 5.79 DD156 pKa = 3.57 CGFACVLPEE165 pKa = 3.8 GHH167 pKa = 7.08 KK168 pKa = 10.02 LAKK171 pKa = 9.78 KK172 pKa = 10.45 RR173 pKa = 11.84 IVTPDD178 pKa = 3.5 DD179 pKa = 3.56 LVGEE183 pKa = 4.27 RR184 pKa = 11.84 LIGSPTDD191 pKa = 3.46 TPYY194 pKa = 11.43 GQALLRR200 pKa = 11.84 AYY202 pKa = 9.84 GPHH205 pKa = 7.13 ASTLNVQSLVRR216 pKa = 11.84 SATSACWQAQAGGGIAVVDD235 pKa = 4.66 RR236 pKa = 11.84 AAVAGTTFRR245 pKa = 11.84 QLVVRR250 pKa = 11.84 PFQTATRR257 pKa = 11.84 LPIAVLHH264 pKa = 5.47 NRR266 pKa = 11.84 YY267 pKa = 9.4 RR268 pKa = 11.84 PLSVVQQAFCDD279 pKa = 3.4 AFAAVWKK286 pKa = 10.6 RR287 pKa = 11.84 EE288 pKa = 3.95 MPRR291 pKa = 3.2
Molecular weight: 31.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.502
IPC_protein 10.482
Toseland 10.584
ProMoST 10.599
Dawson 10.687
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.745
Grimsley 10.745
Solomon 10.847
Lehninger 10.804
Nozaki 10.599
DTASelect 10.438
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.628
Patrickios 10.452
IPC_peptide 10.847
IPC2_peptide 9.721
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5874
0
5874
1496469
24
1916
254.8
27.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.268 ± 0.036
0.949 ± 0.01
5.062 ± 0.026
4.856 ± 0.027
3.572 ± 0.02
8.649 ± 0.031
2.252 ± 0.016
4.234 ± 0.025
3.106 ± 0.027
10.817 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.018
2.563 ± 0.02
5.366 ± 0.028
4.045 ± 0.021
6.637 ± 0.034
5.44 ± 0.025
5.278 ± 0.026
7.693 ± 0.029
1.481 ± 0.014
2.179 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here