Vicugna pacos (Alpaca) (Lama pacos)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36880 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J3BDL2|A0A6J3BDL2_VICPA Isoform of A0A6J3BBS2 CTD small phosphatase-like protein isoform X5 OS=Vicugna pacos OX=30538 GN=CTDSPL PE=4 SV=1
MM1 pKa = 7.36 EE2 pKa = 5.31 AVSLSPYY9 pKa = 10.69 VLGLLLLPLLAVLLMALCVRR29 pKa = 11.84 CRR31 pKa = 11.84 EE32 pKa = 4.25 LPGSYY37 pKa = 8.66 DD38 pKa = 3.31 TAASDD43 pKa = 3.84 SLTPSSIVIKK53 pKa = 10.35 RR54 pKa = 11.84 PPTLAPWPPATSYY67 pKa = 11.11 PPVTSYY73 pKa = 11.3 PPLNQPDD80 pKa = 4.81 LLPIPRR86 pKa = 11.84 SPQPPGGSHH95 pKa = 6.82 RR96 pKa = 11.84 MPSSRR101 pKa = 11.84 QASDD105 pKa = 3.11 GANSVASYY113 pKa = 10.76 EE114 pKa = 4.13 NEE116 pKa = 3.86 EE117 pKa = 4.29 PVCEE121 pKa = 5.65 DD122 pKa = 3.23 EE123 pKa = 7.0 DD124 pKa = 4.15 EE125 pKa = 5.92 DD126 pKa = 4.66 EE127 pKa = 6.11 DD128 pKa = 4.81 EE129 pKa = 4.41 EE130 pKa = 5.69 DD131 pKa = 3.77 YY132 pKa = 11.56 HH133 pKa = 8.4 NEE135 pKa = 4.02 GYY137 pKa = 10.83 LVVLPDD143 pKa = 3.85 SVPATGTTVPPAPVSNNPGPRR164 pKa = 11.84 DD165 pKa = 3.34 SAFSMEE171 pKa = 4.23 SGEE174 pKa = 4.99 DD175 pKa = 3.63 YY176 pKa = 11.66 VNVPEE181 pKa = 4.68 SEE183 pKa = 4.29 EE184 pKa = 4.1 SADD187 pKa = 3.58 ASLDD191 pKa = 3.44 GSRR194 pKa = 11.84 EE195 pKa = 3.99 YY196 pKa = 11.72 VNVSQEE202 pKa = 4.06 LQPVAGTKK210 pKa = 9.68 PATPGSRR217 pKa = 11.84 EE218 pKa = 4.08 VEE220 pKa = 4.17 DD221 pKa = 4.96 EE222 pKa = 4.04 EE223 pKa = 6.11 APDD226 pKa = 4.29 YY227 pKa = 11.55 EE228 pKa = 4.24 NLQPNN233 pKa = 3.81
Molecular weight: 24.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.668
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.681
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.088
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.973
Patrickios 1.125
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A6J0AI98|A0A6J0AI98_VICPA forkhead box protein I3 OS=Vicugna pacos OX=30538 GN=FOXI3 PE=4 SV=1
MM1 pKa = 7.52 SPLKK5 pKa = 9.95 TFRR8 pKa = 11.84 TRR10 pKa = 11.84 RR11 pKa = 11.84 FLVKK15 pKa = 9.92 KK16 pKa = 9.95 QKK18 pKa = 9.18 QNRR21 pKa = 11.84 PTPQWVHH28 pKa = 4.65 MKK30 pKa = 8.71 TRR32 pKa = 11.84 SKK34 pKa = 10.24 IRR36 pKa = 11.84 HH37 pKa = 4.55 NSKK40 pKa = 8.85 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 9.9 WGLL51 pKa = 3.16
Molecular weight: 6.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19465
17415
36880
24174655
31
34501
655.5
73.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.98 ± 0.013
2.081 ± 0.009
4.898 ± 0.009
7.289 ± 0.018
3.451 ± 0.01
6.377 ± 0.017
2.574 ± 0.006
4.197 ± 0.011
5.855 ± 0.02
9.863 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.12 ± 0.005
3.539 ± 0.01
6.376 ± 0.022
4.945 ± 0.012
5.739 ± 0.013
8.579 ± 0.018
5.372 ± 0.011
6.012 ± 0.013
1.155 ± 0.004
2.566 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here