Roseivirga spongicola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Roseivirgaceae; Roseivirga

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150X5K0|A0A150X5K0_9BACT Phenylacetate-CoA oxygenase subunit PaaJ OS=Roseivirga spongicola OX=333140 GN=AWW68_15115 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84ILLVGCFILTCHH14 pKa = 6.21FVCAQGTFTSANSGSWTDD32 pKa = 3.47PGSWTLVGTDD42 pKa = 3.54SDD44 pKa = 4.18GNNYY48 pKa = 9.39PDD50 pKa = 4.61GNDD53 pKa = 2.99NVIINGHH60 pKa = 7.21AITVSSTEE68 pKa = 3.8ATSTLEE74 pKa = 3.82LATGIVEE81 pKa = 4.46FSGAAAQLNVRR92 pKa = 11.84GTLSSSGASEE102 pKa = 4.2VTGTSTNHH110 pKa = 6.48RR111 pKa = 11.84LVIVNNGTTVLGNISVNGGTFTLDD135 pKa = 3.41GARR138 pKa = 11.84LTNNGEE144 pKa = 3.93LTVADD149 pKa = 4.32GATFLIDD156 pKa = 4.76DD157 pKa = 4.06STAPTVVANHH167 pKa = 7.29LFTDD171 pKa = 3.66IDD173 pKa = 3.79INSNGTFQNNDD184 pKa = 3.48AIQIIIRR191 pKa = 11.84GTITNNGTFNGCGDD205 pKa = 3.95DD206 pKa = 3.94SCNYY210 pKa = 8.17TYY212 pKa = 11.32NVAGSFVVDD221 pKa = 3.89GTTTVNMPRR230 pKa = 11.84VLVYY234 pKa = 10.83NGATLTNQGLISTIDD249 pKa = 3.52LLGNGVGVNTFVNEE263 pKa = 3.76GTLTVNTSGTIDD275 pKa = 3.63EE276 pKa = 5.13ADD278 pKa = 3.42LVLDD282 pKa = 4.3FDD284 pKa = 4.83AVGNTLVYY292 pKa = 10.3EE293 pKa = 4.93GSTTEE298 pKa = 3.97QLFSSTFYY306 pKa = 10.57NVEE309 pKa = 3.93VNLEE313 pKa = 4.34DD314 pKa = 3.54GTLINLNNGVSSTINNNLTVTSGDD338 pKa = 3.18ARR340 pKa = 11.84IINSANTLTVLGNLSIEE357 pKa = 4.36GDD359 pKa = 3.37GSFFMNTTSVGEE371 pKa = 4.53GISITGDD378 pKa = 3.03FDD380 pKa = 4.22VSSTNATAVDD390 pKa = 4.7LNNGTITFTNMTITNGADD408 pKa = 3.08VFTGVADD415 pKa = 3.95LTSMGTITVTNGSVNPDD432 pKa = 2.85GTATPTFNNILVEE445 pKa = 4.78ANGSWLSTSVYY456 pKa = 10.87DD457 pKa = 3.83PTITGNITNNGTFTACLNTSGCDD480 pKa = 3.39YY481 pKa = 10.36TLTSGSGTLGGSSTISNMANIFLNDD506 pKa = 3.41GASYY510 pKa = 10.58TNEE513 pKa = 3.74NTSAGGIQVGTGIQTTSGAGSFINGANATLDD544 pKa = 3.68YY545 pKa = 11.18SGTLANFSVTNFTASAAGNTVIFSRR570 pKa = 11.84PDD572 pKa = 3.04ANQSLRR578 pKa = 11.84ATADD582 pKa = 3.18ANNEE586 pKa = 4.17YY587 pKa = 11.35VNVTINKK594 pKa = 9.51ADD596 pKa = 3.69GFDD599 pKa = 4.48VILDD603 pKa = 3.85DD604 pKa = 5.24NITVSGTLTLTAGDD618 pKa = 3.81VQLNAFDD625 pKa = 4.1LTLADD630 pKa = 3.98GATVSGGNSTSYY642 pKa = 10.8IKK644 pKa = 11.14LNGAGLLNQEE654 pKa = 4.27YY655 pKa = 9.76SAVGATLSFPIGDD668 pKa = 3.66VDD670 pKa = 5.19DD671 pKa = 5.07FSPINSLTLHH681 pKa = 6.31SATLGASPSIEE692 pKa = 4.19FDD694 pKa = 2.8ITDD697 pKa = 3.76AANSNRR703 pKa = 11.84DD704 pKa = 3.03VDD706 pKa = 3.85NTGAGGDD713 pKa = 4.12DD714 pKa = 4.96DD715 pKa = 4.61GTAATSFLSRR725 pKa = 11.84FWTLTPTDD733 pKa = 3.43ISDD736 pKa = 3.57VTFSASYY743 pKa = 9.29TYY745 pKa = 11.3VDD747 pKa = 4.08ADD749 pKa = 3.79VAGATANEE757 pKa = 4.09AEE759 pKa = 4.72LVGSVYY765 pKa = 9.29RR766 pKa = 11.84TPIGEE771 pKa = 4.52GFLDD775 pKa = 3.66WSVEE779 pKa = 4.03EE780 pKa = 4.54AVNATSNLVTVSNVSSTALGVLYY803 pKa = 11.06AMDD806 pKa = 3.78NTLEE810 pKa = 4.1RR811 pKa = 11.84LPVEE815 pKa = 4.28LLSFTAITRR824 pKa = 11.84LQEE827 pKa = 4.11VVLNWEE833 pKa = 4.38TTSEE837 pKa = 4.16EE838 pKa = 4.31NNSHH842 pKa = 5.06FTIEE846 pKa = 3.89RR847 pKa = 11.84SANGISFQSIGEE859 pKa = 4.13LEE861 pKa = 4.25GKK863 pKa = 10.34GNSSEE868 pKa = 4.38LISYY872 pKa = 9.31SFIDD876 pKa = 4.07EE877 pKa = 4.43FPLTGRR883 pKa = 11.84AYY885 pKa = 10.69YY886 pKa = 10.08RR887 pKa = 11.84LKK889 pKa = 10.71QVDD892 pKa = 3.77FSGAFEE898 pKa = 3.99YY899 pKa = 11.09SEE901 pKa = 4.74VISAVYY907 pKa = 10.36DD908 pKa = 3.46GAVNYY913 pKa = 9.95NFRR916 pKa = 11.84LFPNPVNSGGEE927 pKa = 3.93LTLSFEE933 pKa = 4.41RR934 pKa = 11.84TQTNSEE940 pKa = 4.23VVKK943 pKa = 9.76WQLLSITGEE952 pKa = 3.85QLIEE956 pKa = 3.91QVGSDD961 pKa = 3.59KK962 pKa = 11.03EE963 pKa = 4.26YY964 pKa = 10.43IIDD967 pKa = 3.74TSKK970 pKa = 11.25LSLGAYY976 pKa = 9.07LVRR979 pKa = 11.84VVYY982 pKa = 10.77SDD984 pKa = 3.71GRR986 pKa = 11.84VDD988 pKa = 3.61SQRR991 pKa = 11.84LVVNN995 pKa = 4.52

Molecular weight: 103.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150XBP5|A0A150XBP5_9BACT Uncharacterized protein OS=Roseivirga spongicola OX=333140 GN=AWW68_10030 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84TTLLSNSIFLITSSFLRR19 pKa = 11.84ISILQKK25 pKa = 9.37YY26 pKa = 9.41RR27 pKa = 11.84GRR29 pKa = 11.84KK30 pKa = 7.84HH31 pKa = 6.25VKK33 pKa = 9.65SEE35 pKa = 3.73NYY37 pKa = 9.46QIRR40 pKa = 11.84EE41 pKa = 4.3LNSSDD46 pKa = 4.1LKK48 pKa = 11.01HH49 pKa = 6.92SNVDD53 pKa = 3.72FWTIAGRR60 pKa = 11.84IAIAAGLLRR69 pKa = 11.84AAEE72 pKa = 4.28DD73 pKa = 3.5VFLRR77 pKa = 11.84GTT79 pKa = 3.46

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3854

0

3854

1330867

52

4624

345.3

38.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.798 ± 0.041

0.654 ± 0.011

5.621 ± 0.034

7.216 ± 0.044

5.09 ± 0.03

6.807 ± 0.044

1.742 ± 0.02

7.104 ± 0.031

6.743 ± 0.058

9.543 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.025

5.395 ± 0.04

3.514 ± 0.021

3.712 ± 0.022

4.029 ± 0.031

6.828 ± 0.04

5.372 ± 0.064

6.499 ± 0.034

1.147 ± 0.015

3.824 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski