Mossman virus
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6WGM3|Q6WGM3_9MONO Isoform of Q6WGM5 C protein OS=Mossman virus OX=241630 GN=C PE=4 SV=1
MM1 pKa = 7.39 SGVLSALRR9 pKa = 11.84 EE10 pKa = 4.13 FKK12 pKa = 10.49 DD13 pKa = 3.52 ARR15 pKa = 11.84 LASKK19 pKa = 11.28 GEE21 pKa = 3.85 GLTRR25 pKa = 11.84 GAITGIKK32 pKa = 9.61 QKK34 pKa = 10.2 IAVIIPGQEE43 pKa = 3.68 GSKK46 pKa = 10.52 VRR48 pKa = 11.84 WQLLRR53 pKa = 11.84 LLLGVIWSQEE63 pKa = 3.61 ASPGVITGAFLSLISLISEE82 pKa = 4.3 SPGNMVRR89 pKa = 11.84 RR90 pKa = 11.84 LNNDD94 pKa = 3.08 PDD96 pKa = 3.75 LAITIIEE103 pKa = 4.19 FTIGPDD109 pKa = 3.31 SEE111 pKa = 4.9 YY112 pKa = 11.13 KK113 pKa = 9.53 FASRR117 pKa = 11.84 GMSYY121 pKa = 10.46 EE122 pKa = 3.87 EE123 pKa = 4.1 QMAHH127 pKa = 5.78 YY128 pKa = 9.87 LHH130 pKa = 7.01 LRR132 pKa = 11.84 DD133 pKa = 4.76 TPPQSAPDD141 pKa = 3.8 DD142 pKa = 4.17 FPFEE146 pKa = 4.07 EE147 pKa = 4.46 SEE149 pKa = 3.91 AWRR152 pKa = 11.84 QDD154 pKa = 3.73 DD155 pKa = 3.67 MPMDD159 pKa = 4.18 EE160 pKa = 5.01 FLVANMTVQVQLWTLLIKK178 pKa = 10.63 AVTAPDD184 pKa = 3.53 TARR187 pKa = 11.84 DD188 pKa = 3.86 GEE190 pKa = 4.08 QRR192 pKa = 11.84 RR193 pKa = 11.84 WLKK196 pKa = 10.05 FVQQRR201 pKa = 11.84 RR202 pKa = 11.84 VEE204 pKa = 4.27 SFYY207 pKa = 11.4 KK208 pKa = 10.63 LHH210 pKa = 5.67 TVWMDD215 pKa = 3.86 RR216 pKa = 11.84 ARR218 pKa = 11.84 MHH220 pKa = 6.64 IAASLSIRR228 pKa = 11.84 RR229 pKa = 11.84 YY230 pKa = 7.29 MVKK233 pKa = 9.14 TLIEE237 pKa = 4.17 IQRR240 pKa = 11.84 MGQGKK245 pKa = 9.37 GRR247 pKa = 11.84 LLEE250 pKa = 4.35 VIADD254 pKa = 3.39 IGNYY258 pKa = 9.27 IEE260 pKa = 4.31 EE261 pKa = 4.44 SGLAGFQLTIRR272 pKa = 11.84 FGIEE276 pKa = 3.36 TKK278 pKa = 10.7 YY279 pKa = 10.9 AALALNEE286 pKa = 4.07 FQGDD290 pKa = 3.14 IATIEE295 pKa = 4.18 RR296 pKa = 11.84 LMKK299 pKa = 10.46 LYY301 pKa = 11.08 LEE303 pKa = 5.38 LGPTAPFMVLLEE315 pKa = 4.94 DD316 pKa = 4.9 SIQTRR321 pKa = 11.84 FAPGNYY327 pKa = 8.54 PLLWSYY333 pKa = 12.11 AMGVGSALDD342 pKa = 3.0 RR343 pKa = 11.84 AMANLNFNRR352 pKa = 11.84 SYY354 pKa = 11.45 LDD356 pKa = 2.93 YY357 pKa = 11.02 GYY359 pKa = 10.82 FRR361 pKa = 11.84 LGYY364 pKa = 9.65 RR365 pKa = 11.84 IVRR368 pKa = 11.84 QSEE371 pKa = 4.36 GSVDD375 pKa = 2.9 TRR377 pKa = 11.84 MARR380 pKa = 11.84 EE381 pKa = 3.8 LGITDD386 pKa = 4.02 EE387 pKa = 4.24 EE388 pKa = 4.34 QQRR391 pKa = 11.84 LRR393 pKa = 11.84 RR394 pKa = 11.84 LVADD398 pKa = 4.47 LGNRR402 pKa = 11.84 GDD404 pKa = 4.22 SEE406 pKa = 3.95 AAAYY410 pKa = 9.91 QGGAFQLANIQDD422 pKa = 4.06 FEE424 pKa = 5.02 NDD426 pKa = 3.27 DD427 pKa = 3.75 AFAANPQQAPRR438 pKa = 11.84 NNRR441 pKa = 11.84 NRR443 pKa = 11.84 RR444 pKa = 11.84 RR445 pKa = 11.84 RR446 pKa = 11.84 GQPDD450 pKa = 2.97 QGDD453 pKa = 4.19 DD454 pKa = 3.75 DD455 pKa = 6.25 DD456 pKa = 6.45 SDD458 pKa = 3.99 EE459 pKa = 5.73 GDD461 pKa = 3.66 DD462 pKa = 4.43 QGGSGYY468 pKa = 10.67 AAAVHH473 pKa = 6.24 AVLHH477 pKa = 5.95 SSGDD481 pKa = 3.55 GGDD484 pKa = 4.49 DD485 pKa = 3.54 LTSGADD491 pKa = 3.69 GSIDD495 pKa = 3.76 PPTSGLGANYY505 pKa = 9.92 RR506 pKa = 11.84 YY507 pKa = 10.45 SPGLNDD513 pKa = 4.82 SKK515 pKa = 11.32 PKK517 pKa = 10.78 FNDD520 pKa = 3.04 ADD522 pKa = 4.19 LLGLGDD528 pKa = 3.76
Molecular weight: 58.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.973
IPC2_protein 4.952
IPC_protein 4.914
Toseland 4.787
ProMoST 4.991
Dawson 4.876
Bjellqvist 5.029
Wikipedia 4.762
Rodwell 4.774
Grimsley 4.698
Solomon 4.876
Lehninger 4.838
Nozaki 4.991
DTASelect 5.181
Thurlkill 4.787
EMBOSS 4.787
Sillero 5.054
Patrickios 4.266
IPC_peptide 4.889
IPC2_peptide 5.041
IPC2.peptide.svr19 5.061
Protein with the highest isoelectric point:
>tr|Q6WGM5|Q6WGM5_9MONO Phosphoprotein OS=Mossman virus OX=241630 GN=C PE=4 SV=1
MM1 pKa = 7.15 ATMHH5 pKa = 6.49 EE6 pKa = 4.7 FLPGTWVNRR15 pKa = 11.84 GLLEE19 pKa = 5.16 DD20 pKa = 3.88 IRR22 pKa = 11.84 PEE24 pKa = 3.78 YY25 pKa = 10.6 DD26 pKa = 2.95 HH27 pKa = 7.03 EE28 pKa = 4.74 GKK30 pKa = 10.13 IKK32 pKa = 10.35 PRR34 pKa = 11.84 VRR36 pKa = 11.84 VIDD39 pKa = 4.02 PGAGTRR45 pKa = 11.84 KK46 pKa = 10.05 SSGFMYY52 pKa = 10.52 LHH54 pKa = 6.31 LQGIIEE60 pKa = 4.15 DD61 pKa = 4.16 WIGDD65 pKa = 3.85 FEE67 pKa = 4.74 PATRR71 pKa = 11.84 PPGRR75 pKa = 11.84 TLAAYY80 pKa = 8.55 PLGVGQSIAGPYY92 pKa = 9.53 EE93 pKa = 4.28 LLTACLEE100 pKa = 4.29 LNVVVRR106 pKa = 11.84 RR107 pKa = 11.84 TVGSTEE113 pKa = 3.66 KK114 pKa = 10.91 VVFYY118 pKa = 11.35 NNAALDD124 pKa = 3.81 VLSPWKK130 pKa = 10.13 NVLMNGCIFDD140 pKa = 3.87 ANKK143 pKa = 8.87 VCKK146 pKa = 9.78 RR147 pKa = 11.84 VEE149 pKa = 4.69 DD150 pKa = 3.57 IPLEE154 pKa = 4.1 KK155 pKa = 10.11 EE156 pKa = 3.69 QRR158 pKa = 11.84 FRR160 pKa = 11.84 PIYY163 pKa = 9.55 LTITLLTDD171 pKa = 3.08 SGLYY175 pKa = 7.73 KK176 pKa = 9.81 TPSMIQDD183 pKa = 2.95 IRR185 pKa = 11.84 ANNAVAFNLLVYY197 pKa = 8.57 LTAGNGIMEE206 pKa = 4.88 KK207 pKa = 10.18 LYY209 pKa = 9.81 PHH211 pKa = 7.02 TGSEE215 pKa = 4.32 PEE217 pKa = 3.9 QTVVSFMVHH226 pKa = 5.06 VGLFMRR232 pKa = 11.84 GRR234 pKa = 11.84 KK235 pKa = 8.78 KK236 pKa = 10.57 AYY238 pKa = 9.08 SHH240 pKa = 7.34 DD241 pKa = 3.74 YY242 pKa = 10.74 CRR244 pKa = 11.84 LKK246 pKa = 10.62 VEE248 pKa = 4.45 RR249 pKa = 11.84 MNLQFGLGGVGGVSFHH265 pKa = 6.32 MRR267 pKa = 11.84 VQGKK271 pKa = 9.45 LSKK274 pKa = 10.44 SLHH277 pKa = 5.67 AQLGFHH283 pKa = 7.16 RR284 pKa = 11.84 SVCYY288 pKa = 10.67 SLMDD292 pKa = 4.56 INPALNKK299 pKa = 10.23 LLWRR303 pKa = 11.84 TQCSIQRR310 pKa = 11.84 VVAVFQPSVPSEE322 pKa = 3.33 FRR324 pKa = 11.84 IYY326 pKa = 11.23 NDD328 pKa = 3.15 VLIDD332 pKa = 3.62 NTGKK336 pKa = 10.49 IMTYY340 pKa = 10.69
Molecular weight: 38.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.476
IPC2_protein 8.404
IPC_protein 8.331
Toseland 8.653
ProMoST 8.858
Dawson 9.092
Bjellqvist 9.121
Wikipedia 9.224
Rodwell 9.18
Grimsley 8.975
Solomon 9.194
Lehninger 9.165
Nozaki 9.194
DTASelect 8.96
Thurlkill 9.033
EMBOSS 9.238
Sillero 9.253
Patrickios 4.418
IPC_peptide 9.194
IPC2_peptide 7.98
IPC2.peptide.svr19 7.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5246
152
2205
655.8
73.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.538 ± 0.43
1.639 ± 0.303
5.471 ± 0.349
5.623 ± 0.601
2.993 ± 0.409
6.348 ± 0.721
2.402 ± 0.506
7.301 ± 0.743
4.727 ± 0.366
9.379 ± 0.622
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.213
5.032 ± 0.311
4.918 ± 0.211
4.175 ± 0.412
5.49 ± 0.465
8.254 ± 0.678
6.271 ± 0.641
6.176 ± 0.497
1.106 ± 0.186
3.565 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here