Asparagus virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8RCA5|B8RCA5_9BROM 2b protein OS=Asparagus virus 2 OX=39681 GN=2b PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 3.62AHH4 pKa = 6.46YY5 pKa = 9.92FLSEE9 pKa = 3.86LCDD12 pKa = 3.05IRR14 pKa = 11.84KK15 pKa = 8.66FVSTRR20 pKa = 11.84VIEE23 pKa = 3.91QSFDD27 pKa = 3.26IKK29 pKa = 11.08DD30 pKa = 4.1DD31 pKa = 3.54EE32 pKa = 4.68TTSYY36 pKa = 10.05LQAWMYY42 pKa = 11.19SRR44 pKa = 11.84ILVPVMKK51 pKa = 10.27FHH53 pKa = 7.66LCGEE57 pKa = 4.21VLLDD61 pKa = 4.33KK62 pKa = 11.03IEE64 pKa = 4.36DD65 pKa = 3.79VFRR68 pKa = 11.84EE69 pKa = 4.11TLGCEE74 pKa = 4.33AEE76 pKa = 4.4PNDD79 pKa = 4.6DD80 pKa = 3.22GYY82 pKa = 11.1MPVFIHH88 pKa = 7.44LDD90 pKa = 3.74DD91 pKa = 6.5DD92 pKa = 5.06DD93 pKa = 6.12DD94 pKa = 4.39EE95 pKa = 5.29PPPEE99 pKa = 4.93AYY101 pKa = 10.14LPKK104 pKa = 10.23EE105 pKa = 3.87EE106 pKa = 5.0RR107 pKa = 11.84EE108 pKa = 4.47AIHH111 pKa = 6.68AATFLGHH118 pKa = 5.63EE119 pKa = 4.36HH120 pKa = 6.52WASVTSGSDD129 pKa = 3.25FYY131 pKa = 11.91DD132 pKa = 4.94DD133 pKa = 4.71IEE135 pKa = 4.25MLADD139 pKa = 3.27EE140 pKa = 5.39TIYY143 pKa = 11.49DD144 pKa = 4.16DD145 pKa = 5.31LQTDD149 pKa = 3.75KK150 pKa = 10.81CAEE153 pKa = 4.07MPADD157 pKa = 4.06YY158 pKa = 10.89QIMWNDD164 pKa = 3.57GAIDD168 pKa = 4.21AVWSSQFEE176 pKa = 4.49CEE178 pKa = 4.45EE179 pKa = 3.97PPKK182 pKa = 10.65PKK184 pKa = 10.4FKK186 pKa = 10.19PQSVDD191 pKa = 2.99KK192 pKa = 11.15GCDD195 pKa = 3.21PVVIQAAIDD204 pKa = 4.38DD205 pKa = 4.42IFPFHH210 pKa = 7.96HH211 pKa = 6.58EE212 pKa = 3.42MDD214 pKa = 3.33DD215 pKa = 4.56RR216 pKa = 11.84YY217 pKa = 10.34FQTLVEE223 pKa = 4.32TQDD226 pKa = 2.99ISLEE230 pKa = 4.17VSKK233 pKa = 10.97CWIDD237 pKa = 3.22ASNFRR242 pKa = 11.84DD243 pKa = 4.1FVKK246 pKa = 10.9GQDD249 pKa = 3.44SYY251 pKa = 11.95AVPVIQSGATSKK263 pKa = 10.43RR264 pKa = 11.84VHH266 pKa = 5.39TQRR269 pKa = 11.84EE270 pKa = 3.7ALLAVKK276 pKa = 9.4KK277 pKa = 10.59RR278 pKa = 11.84NMNIPEE284 pKa = 4.22LQSTFDD290 pKa = 4.64LDD292 pKa = 4.98AEE294 pKa = 4.56VNTCFKK300 pKa = 10.81RR301 pKa = 11.84FLTHH305 pKa = 6.18VVDD308 pKa = 4.24VPRR311 pKa = 11.84LRR313 pKa = 11.84RR314 pKa = 11.84LQPMTGVEE322 pKa = 4.04VEE324 pKa = 4.59FFNQYY329 pKa = 11.22LLGKK333 pKa = 9.63NPPLKK338 pKa = 10.01EE339 pKa = 4.06YY340 pKa = 10.53QGPLPLCSLDD350 pKa = 4.25KK351 pKa = 10.73YY352 pKa = 9.09MHH354 pKa = 5.51MVKK357 pKa = 9.98TIVKK361 pKa = 8.75PVEE364 pKa = 4.43DD365 pKa = 3.85NSLKK369 pKa = 10.61YY370 pKa = 10.06EE371 pKa = 4.23RR372 pKa = 11.84PLCATITYY380 pKa = 8.26HH381 pKa = 6.97KK382 pKa = 9.85KK383 pKa = 10.32GIVMQSSPLFLSAMSRR399 pKa = 11.84LFYY402 pKa = 10.61VLKK405 pKa = 10.64SKK407 pKa = 10.19IHH409 pKa = 5.86IPSGKK414 pKa = 7.2WHH416 pKa = 6.3QLFTLDD422 pKa = 5.07AEE424 pKa = 4.7KK425 pKa = 10.61FDD427 pKa = 3.55AAKK430 pKa = 9.69WFKK433 pKa = 10.72EE434 pKa = 3.7VDD436 pKa = 3.71FSKK439 pKa = 10.64FDD441 pKa = 3.3KK442 pKa = 11.19SQGEE446 pKa = 4.14LHH448 pKa = 6.89HH449 pKa = 6.75KK450 pKa = 8.67VQEE453 pKa = 4.67KK454 pKa = 10.38IFEE457 pKa = 4.32CLKK460 pKa = 10.44LPPGFVEE467 pKa = 4.1MWFTALEE474 pKa = 4.04RR475 pKa = 11.84SHH477 pKa = 8.3IVDD480 pKa = 3.93RR481 pKa = 11.84EE482 pKa = 3.95AGIGFSVDD490 pKa = 3.65FQRR493 pKa = 11.84RR494 pKa = 11.84TGDD497 pKa = 3.07ANTYY501 pKa = 10.16LGNTLVTLICLARR514 pKa = 11.84VYY516 pKa = 10.41DD517 pKa = 4.03LCNPNITFVIASGDD531 pKa = 3.51DD532 pKa = 3.61SLIGSVDD539 pKa = 3.48EE540 pKa = 4.67LPRR543 pKa = 11.84GPEE546 pKa = 3.88DD547 pKa = 4.79LFTTLFNFEE556 pKa = 4.52AKK558 pKa = 10.1FPHH561 pKa = 5.93NQPFICSKK569 pKa = 10.53FLVSVDD575 pKa = 3.54LADD578 pKa = 4.2GGRR581 pKa = 11.84EE582 pKa = 4.11VIAVPNPAKK591 pKa = 10.65LLIRR595 pKa = 11.84MGRR598 pKa = 11.84RR599 pKa = 11.84DD600 pKa = 3.97CQYY603 pKa = 10.86QALDD607 pKa = 3.78DD608 pKa = 5.92LYY610 pKa = 10.84TSWLDD615 pKa = 3.25VIYY618 pKa = 10.19YY619 pKa = 10.13FRR621 pKa = 11.84DD622 pKa = 3.06SRR624 pKa = 11.84VCEE627 pKa = 4.17KK628 pKa = 10.72VASLCAFRR636 pKa = 11.84QTRR639 pKa = 11.84RR640 pKa = 11.84SSMYY644 pKa = 10.48LLSALLSLPSRR655 pKa = 11.84FANKK659 pKa = 10.05KK660 pKa = 8.24KK661 pKa = 10.66FKK663 pKa = 8.91WLCYY667 pKa = 9.73HH668 pKa = 6.61LTEE671 pKa = 4.61QEE673 pKa = 4.14CLKK676 pKa = 9.5ITKK679 pKa = 9.95QPGSSHH685 pKa = 4.08YY686 pKa = 9.02TKK688 pKa = 10.76KK689 pKa = 10.72NEE691 pKa = 4.02CSKK694 pKa = 10.9EE695 pKa = 4.19SVKK698 pKa = 10.76NFPHH702 pKa = 6.62RR703 pKa = 11.84RR704 pKa = 11.84SSCGRR709 pKa = 11.84WEE711 pKa = 5.18FIPKK715 pKa = 9.96IKK717 pKa = 10.64SDD719 pKa = 3.46DD720 pKa = 3.84DD721 pKa = 3.52YY722 pKa = 11.72RR723 pKa = 11.84IFPNGDD729 pKa = 3.02LSTIRR734 pKa = 11.84GSSVSKK740 pKa = 9.72TDD742 pKa = 3.31CGYY745 pKa = 11.31GKK747 pKa = 10.76CSDD750 pKa = 3.74TASSIGNKK758 pKa = 6.8NTRR761 pKa = 11.84PRR763 pKa = 11.84NRR765 pKa = 11.84FRR767 pKa = 11.84AEE769 pKa = 3.98GVHH772 pKa = 5.76QPSGCYY778 pKa = 9.66SNRR781 pKa = 11.84LPPNHH786 pKa = 6.59RR787 pKa = 11.84RR788 pKa = 11.84SSS790 pKa = 3.3

Molecular weight: 90.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65299|Q65299_9BROM Coat protein OS=Asparagus virus 2 OX=39681 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.53SGNAIEE7 pKa = 5.15VNGRR11 pKa = 11.84WYY13 pKa = 9.75TPAPNNAPTRR23 pKa = 11.84GRR25 pKa = 11.84GGRR28 pKa = 11.84RR29 pKa = 11.84QPTARR34 pKa = 11.84SRR36 pKa = 11.84QWAQGQANARR46 pKa = 11.84RR47 pKa = 11.84SQPQALMIGSVPTSLPTWKK66 pKa = 10.28SFPGEE71 pKa = 3.73QWHH74 pKa = 6.02EE75 pKa = 3.77VSGYY79 pKa = 10.36SFPNSWGSGTLAWLTMANEE98 pKa = 4.02LNKK101 pKa = 10.19IKK103 pKa = 9.63TLHH106 pKa = 6.71DD107 pKa = 3.35TTKK110 pKa = 10.29VYY112 pKa = 11.23SVMLGFTCNYY122 pKa = 9.62DD123 pKa = 3.24GYY125 pKa = 11.09AGFVEE130 pKa = 5.47GFSTSNQIHH139 pKa = 7.35PIAPDD144 pKa = 3.97RR145 pKa = 11.84IRR147 pKa = 11.84VKK149 pKa = 10.22KK150 pKa = 10.01GKK152 pKa = 10.24YY153 pKa = 8.57GARR156 pKa = 11.84QQVFPTGTTVSEE168 pKa = 4.53VRR170 pKa = 11.84SNWTFVWNFDD180 pKa = 3.53AAPPTGSKK188 pKa = 10.65DD189 pKa = 3.39AITVTKK195 pKa = 9.51FYY197 pKa = 10.44IATSPLPGVKK207 pKa = 9.77PPSNFLVVEE216 pKa = 4.35EE217 pKa = 4.43

Molecular weight: 23.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2557

193

1076

511.4

57.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.118 ± 0.683

1.995 ± 0.496

6.844 ± 0.888

5.514 ± 0.504

4.654 ± 0.562

5.084 ± 0.703

2.307 ± 0.44

5.436 ± 0.556

6.453 ± 0.323

8.174 ± 0.601

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.222

3.715 ± 0.487

5.28 ± 0.839

2.816 ± 0.411

5.984 ± 0.198

7.822 ± 0.344

6.023 ± 0.631

7.352 ± 0.493

1.525 ± 0.351

3.285 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski