Acidisarcina polymorpha

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Acidisarcina

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7005 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z5G851|A0A2Z5G851_9BACT Uncharacterized protein OS=Acidisarcina polymorpha OX=2211140 GN=ACPOL_5508 PE=4 SV=1
MM1 pKa = 7.7LAPGIRR7 pKa = 11.84GSAAAGTATTLAVTSGGSATTTVAAGSAITLTAKK41 pKa = 8.61VTSGAMALTTGQVNFCDD58 pKa = 4.34ASVPYY63 pKa = 9.35CTDD66 pKa = 2.42IHH68 pKa = 7.54SLGTAQLTSAGTAIMRR84 pKa = 11.84LIPGIGSHH92 pKa = 5.73SYY94 pKa = 10.45KK95 pKa = 10.57VVFSGTIAYY104 pKa = 7.63STSSSAASSLAVTFAGPYY122 pKa = 8.79TSATTFTSSGNPGGYY137 pKa = 7.8TLTASVVGSPRR148 pKa = 11.84SATVAPTGVISFLNTTSNNQVVTTATLTPSAQPLSYY184 pKa = 11.14SMPDD188 pKa = 3.06PGVYY192 pKa = 9.72ILNPSAMVTGDD203 pKa = 3.48FNEE206 pKa = 4.61DD207 pKa = 3.31GKK209 pKa = 10.27PDD211 pKa = 3.95LLLSGIDD218 pKa = 3.82SNFQSSGFAMALGNGDD234 pKa = 3.46GTFTHH239 pKa = 5.91STVANITEE247 pKa = 4.48NEE249 pKa = 3.98TVMAVGDD256 pKa = 4.06FNQDD260 pKa = 2.45GHH262 pKa = 9.11LDD264 pKa = 3.57VAALGSSGLTILLGNGDD281 pKa = 3.61GTFNNNAIVVTPSVPEE297 pKa = 3.68YY298 pKa = 10.27ALWVAGDD305 pKa = 3.65FSGDD309 pKa = 3.72GILDD313 pKa = 4.09LAYY316 pKa = 9.94TNIPLPGVTVLLGNGDD332 pKa = 3.8GTFAAPITGTTAPADD347 pKa = 3.85GALVTGDD354 pKa = 3.64FNGDD358 pKa = 3.25GKK360 pKa = 11.3LDD362 pKa = 3.94LALSSPANGTITVLLGQGNGTFVAAAPVSLVNADD396 pKa = 4.45PIVTGDD402 pKa = 3.44FNGDD406 pKa = 3.23GKK408 pKa = 11.27LDD410 pKa = 3.74LAAASSDD417 pKa = 3.48QTLTLL422 pKa = 3.9

Molecular weight:
41.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z5FX03|A0A2Z5FX03_9BACT Multi-sensor hybrid histidine kinase OS=Acidisarcina polymorpha OX=2211140 GN=ACPOL_1580 PE=4 SV=1
MM1 pKa = 7.66ASNSRR6 pKa = 11.84RR7 pKa = 11.84VIRR10 pKa = 11.84IGRR13 pKa = 11.84VVQGNPPGGQTHH25 pKa = 7.23WIGRR29 pKa = 11.84VQPFQNIRR37 pKa = 11.84QQ38 pKa = 3.64

Molecular weight:
4.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7005

0

7005

2052594

37

2598

293.0

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.615 ± 0.038

0.876 ± 0.009

5.128 ± 0.022

5.504 ± 0.034

3.965 ± 0.021

8.029 ± 0.027

2.244 ± 0.015

5.136 ± 0.02

3.62 ± 0.027

10.054 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.136 ± 0.016

3.421 ± 0.031

5.304 ± 0.024

3.869 ± 0.02

6.199 ± 0.035

6.831 ± 0.031

5.838 ± 0.034

7.063 ± 0.022

1.377 ± 0.013

2.791 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski