Actinoplanes sp. ATCC 53533

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; unclassified Actinoplanes

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11557 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A428YRF3|A0A428YRF3_9ACTN ArsR family transcriptional regulator OS=Actinoplanes sp. ATCC 53533 OX=1288362 GN=DMB66_07285 PE=4 SV=1
MM1 pKa = 7.58SDD3 pKa = 2.83EE4 pKa = 5.08HH5 pKa = 6.41GWPDD9 pKa = 4.02FSDD12 pKa = 3.85PDD14 pKa = 4.0GPEE17 pKa = 3.77DD18 pKa = 3.48HH19 pKa = 7.37HH20 pKa = 8.04DD21 pKa = 3.79HH22 pKa = 6.9GGHH25 pKa = 6.59GDD27 pKa = 3.58QFGTPAAEE35 pKa = 4.32ADD37 pKa = 4.08SPFHH41 pKa = 7.46DD42 pKa = 5.13DD43 pKa = 5.09VFGHH47 pKa = 7.68DD48 pKa = 4.64DD49 pKa = 3.57DD50 pKa = 4.42QAQGFAGHH58 pKa = 7.27DD59 pKa = 3.7LDD61 pKa = 5.97GGPDD65 pKa = 3.63LDD67 pKa = 6.17GIDD70 pKa = 4.22GGHH73 pKa = 6.7GLDD76 pKa = 4.24EE77 pKa = 4.62SQDD80 pKa = 3.38AAGWDD85 pKa = 3.56DD86 pKa = 4.19HH87 pKa = 7.1EE88 pKa = 5.91PGTGHH93 pKa = 7.89DD94 pKa = 4.19DD95 pKa = 3.47AADD98 pKa = 3.79AGVVDD103 pKa = 4.31VTDD106 pKa = 3.81GHH108 pKa = 5.8TMGPVGADD116 pKa = 3.17PDD118 pKa = 3.73AATDD122 pKa = 4.09FEE124 pKa = 4.7QVSLFPPAVDD134 pKa = 3.25VGPLPEE140 pKa = 4.99PVDD143 pKa = 3.67GFPWIDD149 pKa = 3.27TGSLGLYY156 pKa = 10.4HH157 pKa = 7.15SGLDD161 pKa = 3.43HH162 pKa = 7.5AALAEE167 pKa = 4.18PAAEE171 pKa = 3.83PVAAQDD177 pKa = 3.64LAAYY181 pKa = 9.35AAEE184 pKa = 4.49DD185 pKa = 4.25LPPGVDD191 pKa = 3.2PWAALADD198 pKa = 3.91SDD200 pKa = 5.74DD201 pKa = 4.3PATSALAKK209 pKa = 8.62WWHH212 pKa = 6.48PEE214 pKa = 3.62SS215 pKa = 3.85

Molecular weight: 22.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A428YW62|A0A428YW62_9ACTN GGDEF-domain containing protein OS=Actinoplanes sp. ATCC 53533 OX=1288362 GN=DMB66_01575 PE=4 SV=1
MM1 pKa = 7.65AAPARR6 pKa = 11.84TSPRR10 pKa = 11.84RR11 pKa = 11.84ARR13 pKa = 11.84PPWPTPPARR22 pKa = 11.84RR23 pKa = 11.84PATRR27 pKa = 11.84RR28 pKa = 11.84SRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84PPRR35 pKa = 11.84LRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84PPRR45 pKa = 11.84RR46 pKa = 11.84SQRR49 pKa = 11.84AARR52 pKa = 11.84SPPTPCWRR60 pKa = 11.84SS61 pKa = 2.96

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11557

0

11557

3748102

15

4779

324.3

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.149 ± 0.037

0.76 ± 0.007

6.002 ± 0.018

4.85 ± 0.021

2.752 ± 0.014

9.265 ± 0.023

2.152 ± 0.01

3.597 ± 0.013

1.827 ± 0.015

10.353 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.678 ± 0.009

1.938 ± 0.013

6.01 ± 0.02

2.858 ± 0.013

8.142 ± 0.028

5.03 ± 0.017

6.319 ± 0.026

8.599 ± 0.02

1.6 ± 0.01

2.117 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski