Amino acid dipepetide frequency for Actinoplanes sp. ATCC 53533

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.008AlaAla: 24.008 ± 0.143
1.025AlaCys: 1.025 ± 0.02
8.703AlaAsp: 8.703 ± 0.049
8.213AlaGlu: 8.213 ± 0.066
3.445AlaPhe: 3.445 ± 0.031
15.012AlaGly: 15.012 ± 0.08
2.526AlaHis: 2.526 ± 0.027
4.497AlaIle: 4.497 ± 0.042
2.574AlaLys: 2.574 ± 0.035
14.112AlaLeu: 14.112 ± 0.095
2.599AlaMet: 2.599 ± 0.028
2.363AlaAsn: 2.363 ± 0.031
6.687AlaPro: 6.687 ± 0.05
3.805AlaGln: 3.805 ± 0.037
10.265AlaArg: 10.265 ± 0.065
5.814AlaSer: 5.814 ± 0.044
8.303AlaThr: 8.303 ± 0.063
12.711AlaVal: 12.711 ± 0.069
2.018AlaTrp: 2.018 ± 0.022
2.814AlaTyr: 2.814 ± 0.031
0.001AlaXaa: 0.001 ± 0.0
Cys
1.005CysAla: 1.005 ± 0.017
0.101CysCys: 0.101 ± 0.006
0.483CysAsp: 0.483 ± 0.013
0.338CysGlu: 0.338 ± 0.009
0.207CysPhe: 0.207 ± 0.008
0.894CysGly: 0.894 ± 0.019
0.195CysHis: 0.195 ± 0.007
0.153CysIle: 0.153 ± 0.007
0.095CysLys: 0.095 ± 0.005
0.727CysLeu: 0.727 ± 0.016
0.117CysMet: 0.117 ± 0.006
0.134CysAsn: 0.134 ± 0.006
0.482CysPro: 0.482 ± 0.011
0.184CysGln: 0.184 ± 0.007
0.661CysArg: 0.661 ± 0.013
0.41CysSer: 0.41 ± 0.012
0.503CysThr: 0.503 ± 0.015
0.604CysVal: 0.604 ± 0.014
0.14CysTrp: 0.14 ± 0.007
0.169CysTyr: 0.169 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.409AspAla: 7.409 ± 0.048
0.393AspCys: 0.393 ± 0.011
3.92AspAsp: 3.92 ± 0.041
3.524AspGlu: 3.524 ± 0.036
1.581AspPhe: 1.581 ± 0.024
6.252AspGly: 6.252 ± 0.052
1.33AspHis: 1.33 ± 0.02
1.884AspIle: 1.884 ± 0.025
1.078AspLys: 1.078 ± 0.021
6.678AspLeu: 6.678 ± 0.044
0.75AspMet: 0.75 ± 0.016
1.175AspAsn: 1.175 ± 0.021
4.64AspPro: 4.64 ± 0.034
1.836AspGln: 1.836 ± 0.023
5.165AspArg: 5.165 ± 0.037
2.463AspSer: 2.463 ± 0.029
3.011AspThr: 3.011 ± 0.031
4.777AspVal: 4.777 ± 0.041
0.963AspTrp: 0.963 ± 0.015
1.193AspTyr: 1.193 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
6.285GluAla: 6.285 ± 0.053
0.305GluCys: 0.305 ± 0.01
2.083GluAsp: 2.083 ± 0.026
2.237GluGlu: 2.237 ± 0.029
1.397GluPhe: 1.397 ± 0.02
2.958GluGly: 2.958 ± 0.031
1.371GluHis: 1.371 ± 0.02
2.232GluIle: 2.232 ± 0.027
0.984GluLys: 0.984 ± 0.017
6.167GluLeu: 6.167 ± 0.049
0.826GluMet: 0.826 ± 0.016
0.858GluAsn: 0.858 ± 0.016
3.199GluPro: 3.199 ± 0.03
2.201GluGln: 2.201 ± 0.027
4.599GluArg: 4.599 ± 0.039
2.247GluSer: 2.247 ± 0.027
2.557GluThr: 2.557 ± 0.026
4.278GluVal: 4.278 ± 0.035
0.707GluTrp: 0.707 ± 0.014
1.006GluTyr: 1.006 ± 0.016
0.0GluXaa: 0.0 ± 0.0
Phe
3.94PheAla: 3.94 ± 0.029
0.254PheCys: 0.254 ± 0.008
2.067PheAsp: 2.067 ± 0.025
1.247PheGlu: 1.247 ± 0.017
0.856PhePhe: 0.856 ± 0.018
2.948PheGly: 2.948 ± 0.033
0.565PheHis: 0.565 ± 0.012
0.816PheIle: 0.816 ± 0.015
0.46PheLys: 0.46 ± 0.012
2.563PheLeu: 2.563 ± 0.03
0.356PheMet: 0.356 ± 0.01
0.606PheAsn: 0.606 ± 0.015
1.349PhePro: 1.349 ± 0.019
0.705PheGln: 0.705 ± 0.014
1.865PheArg: 1.865 ± 0.022
1.444PheSer: 1.444 ± 0.022
2.066PheThr: 2.066 ± 0.024
2.367PheVal: 2.367 ± 0.028
0.429PheTrp: 0.429 ± 0.012
0.616PheTyr: 0.616 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
10.95GlyAla: 10.95 ± 0.064
0.829GlyCys: 0.829 ± 0.015
5.346GlyAsp: 5.346 ± 0.042
4.412GlyGlu: 4.412 ± 0.033
2.882GlyPhe: 2.882 ± 0.029
8.471GlyGly: 8.471 ± 0.072
2.14GlyHis: 2.14 ± 0.025
3.461GlyIle: 3.461 ± 0.032
2.184GlyLys: 2.184 ± 0.03
9.37GlyLeu: 9.37 ± 0.061
1.821GlyMet: 1.821 ± 0.023
1.918GlyAsn: 1.918 ± 0.03
5.073GlyPro: 5.073 ± 0.036
2.747GlyGln: 2.747 ± 0.026
7.677GlyArg: 7.677 ± 0.057
5.242GlySer: 5.242 ± 0.043
6.142GlyThr: 6.142 ± 0.055
7.549GlyVal: 7.549 ± 0.056
1.927GlyTrp: 1.927 ± 0.027
2.508GlyTyr: 2.508 ± 0.026
0.0GlyXaa: 0.0 ± 0.0
His
2.57HisAla: 2.57 ± 0.032
0.186HisCys: 0.186 ± 0.007
1.377HisAsp: 1.377 ± 0.019
1.076HisGlu: 1.076 ± 0.016
0.574HisPhe: 0.574 ± 0.013
2.106HisGly: 2.106 ± 0.025
0.647HisHis: 0.647 ± 0.014
0.642HisIle: 0.642 ± 0.013
0.298HisLys: 0.298 ± 0.009
2.419HisLeu: 2.419 ± 0.029
0.263HisMet: 0.263 ± 0.008
0.407HisAsn: 0.407 ± 0.012
1.665HisPro: 1.665 ± 0.022
0.665HisGln: 0.665 ± 0.013
2.134HisArg: 2.134 ± 0.025
0.917HisSer: 0.917 ± 0.016
1.169HisThr: 1.169 ± 0.019
1.599HisVal: 1.599 ± 0.02
0.339HisTrp: 0.339 ± 0.011
0.469HisTyr: 0.469 ± 0.012
0.0HisXaa: 0.0 ± 0.0
Ile
5.492IleAla: 5.492 ± 0.041
0.308IleCys: 0.308 ± 0.01
2.674IleAsp: 2.674 ± 0.025
2.011IleGlu: 2.011 ± 0.022
0.93IlePhe: 0.93 ± 0.017
3.85IleGly: 3.85 ± 0.036
0.619IleHis: 0.619 ± 0.014
1.173IleIle: 1.173 ± 0.023
0.719IleLys: 0.719 ± 0.015
2.768IleLeu: 2.768 ± 0.034
0.512IleMet: 0.512 ± 0.012
0.88IleAsn: 0.88 ± 0.016
1.785IlePro: 1.785 ± 0.023
0.79IleGln: 0.79 ± 0.016
2.643IleArg: 2.643 ± 0.028
1.924IleSer: 1.924 ± 0.024
2.532IleThr: 2.532 ± 0.026
3.242IleVal: 3.242 ± 0.027
0.474IleTrp: 0.474 ± 0.012
0.646IleTyr: 0.646 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.419LysAla: 2.419 ± 0.035
0.122LysCys: 0.122 ± 0.006
0.955LysAsp: 0.955 ± 0.017
0.738LysGlu: 0.738 ± 0.014
0.47LysPhe: 0.47 ± 0.011
1.308LysGly: 1.308 ± 0.019
0.372LysHis: 0.372 ± 0.01
0.88LysIle: 0.88 ± 0.017
0.574LysLys: 0.574 ± 0.017
1.936LysLeu: 1.936 ± 0.024
0.324LysMet: 0.324 ± 0.009
0.458LysAsn: 0.458 ± 0.013
1.221LysPro: 1.221 ± 0.018
0.659LysGln: 0.659 ± 0.014
1.279LysArg: 1.279 ± 0.02
1.0LysSer: 1.0 ± 0.018
1.219LysThr: 1.219 ± 0.022
1.66LysVal: 1.66 ± 0.023
0.265LysTrp: 0.265 ± 0.01
0.416LysTyr: 0.416 ± 0.011
0.0LysXaa: 0.0 ± 0.0
Leu
16.068LeuAla: 16.068 ± 0.103
0.775LeuCys: 0.775 ± 0.016
6.551LeuAsp: 6.551 ± 0.047
3.884LeuGlu: 3.884 ± 0.036
2.62LeuPhe: 2.62 ± 0.032
9.227LeuGly: 9.227 ± 0.058
2.229LeuHis: 2.229 ± 0.029
3.992LeuIle: 3.992 ± 0.037
1.588LeuLys: 1.588 ± 0.02
11.286LeuLeu: 11.286 ± 0.085
1.482LeuMet: 1.482 ± 0.021
1.809LeuAsn: 1.809 ± 0.022
6.325LeuPro: 6.325 ± 0.048
2.196LeuGln: 2.196 ± 0.025
9.017LeuArg: 9.017 ± 0.057
5.475LeuSer: 5.475 ± 0.042
7.162LeuThr: 7.162 ± 0.046
8.842LeuVal: 8.842 ± 0.062
1.273LeuTrp: 1.273 ± 0.018
1.726LeuTyr: 1.726 ± 0.023
0.0LeuXaa: 0.0 ± 0.0
Met
2.137MetAla: 2.137 ± 0.027
0.11MetCys: 0.11 ± 0.006
0.755MetAsp: 0.755 ± 0.013
0.563MetGlu: 0.563 ± 0.012
0.489MetPhe: 0.489 ± 0.011
1.063MetGly: 1.063 ± 0.02
0.331MetHis: 0.331 ± 0.01
0.787MetIle: 0.787 ± 0.017
0.352MetLys: 0.352 ± 0.01
1.859MetLeu: 1.859 ± 0.023
0.296MetMet: 0.296 ± 0.008
0.388MetAsn: 0.388 ± 0.01
1.143MetPro: 1.143 ± 0.018
0.418MetGln: 0.418 ± 0.01
1.476MetArg: 1.476 ± 0.02
1.187MetSer: 1.187 ± 0.018
1.566MetThr: 1.566 ± 0.021
1.333MetVal: 1.333 ± 0.02
0.229MetTrp: 0.229 ± 0.008
0.299MetTyr: 0.299 ± 0.009
0.0MetXaa: 0.0 ± 0.0
Asn
2.44AsnAla: 2.44 ± 0.029
0.174AsnCys: 0.174 ± 0.007
1.102AsnAsp: 1.102 ± 0.021
0.834AsnGlu: 0.834 ± 0.015
0.535AsnPhe: 0.535 ± 0.013
2.071AsnGly: 2.071 ± 0.031
0.417AsnHis: 0.417 ± 0.012
0.64AsnIle: 0.64 ± 0.013
0.396AsnLys: 0.396 ± 0.013
1.907AsnLeu: 1.907 ± 0.025
0.293AsnMet: 0.293 ± 0.008
0.533AsnAsn: 0.533 ± 0.014
1.52AsnPro: 1.52 ± 0.022
0.603AsnGln: 0.603 ± 0.017
1.46AsnArg: 1.46 ± 0.021
0.982AsnSer: 0.982 ± 0.018
1.2AsnThr: 1.2 ± 0.02
1.5AsnVal: 1.5 ± 0.021
0.324AsnTrp: 0.324 ± 0.011
0.447AsnTyr: 0.447 ± 0.011
0.0AsnXaa: 0.0 ± 0.0
Pro
9.816ProAla: 9.816 ± 0.06
0.309ProCys: 0.309 ± 0.009
4.386ProAsp: 4.386 ± 0.036
3.45ProGlu: 3.45 ± 0.035
1.492ProPhe: 1.492 ± 0.023
6.583ProGly: 6.583 ± 0.046
1.19ProHis: 1.19 ± 0.019
1.776ProIle: 1.776 ± 0.022
0.962ProLys: 0.962 ± 0.017
4.981ProLeu: 4.981 ± 0.039
1.022ProMet: 1.022 ± 0.018
1.018ProAsn: 1.018 ± 0.017
3.745ProPro: 3.745 ± 0.045
1.594ProGln: 1.594 ± 0.023
3.876ProArg: 3.876 ± 0.037
2.991ProSer: 2.991 ± 0.03
3.443ProThr: 3.443 ± 0.038
5.342ProVal: 5.342 ± 0.041
0.91ProTrp: 0.91 ± 0.016
1.212ProTyr: 1.212 ± 0.018
0.001ProXaa: 0.001 ± 0.0
Gln
4.022GlnAla: 4.022 ± 0.034
0.164GlnCys: 0.164 ± 0.006
1.248GlnAsp: 1.248 ± 0.018
1.177GlnGlu: 1.177 ± 0.016
0.837GlnPhe: 0.837 ± 0.015
1.908GlnGly: 1.908 ± 0.025
0.68GlnHis: 0.68 ± 0.015
1.231GlnIle: 1.231 ± 0.018
0.473GlnLys: 0.473 ± 0.013
3.245GlnLeu: 3.245 ± 0.032
0.458GlnMet: 0.458 ± 0.01
0.556GlnAsn: 0.556 ± 0.016
1.973GlnPro: 1.973 ± 0.026
1.328GlnGln: 1.328 ± 0.024
2.667GlnArg: 2.667 ± 0.028
1.351GlnSer: 1.351 ± 0.021
1.465GlnThr: 1.465 ± 0.022
2.686GlnVal: 2.686 ± 0.027
0.529GlnTrp: 0.529 ± 0.014
0.584GlnTyr: 0.584 ± 0.012
0.0GlnXaa: 0.0 ± 0.0
Arg
10.055ArgAla: 10.055 ± 0.061
0.629ArgCys: 0.629 ± 0.013
4.558ArgAsp: 4.558 ± 0.038
4.048ArgGlu: 4.048 ± 0.036
2.378ArgPhe: 2.378 ± 0.028
5.692ArgGly: 5.692 ± 0.044
2.163ArgHis: 2.163 ± 0.027
3.447ArgIle: 3.447 ± 0.032
1.398ArgLys: 1.398 ± 0.02
9.205ArgLeu: 9.205 ± 0.066
1.789ArgMet: 1.789 ± 0.022
1.474ArgAsn: 1.474 ± 0.023
5.008ArgPro: 5.008 ± 0.045
2.663ArgGln: 2.663 ± 0.031
8.261ArgArg: 8.261 ± 0.069
4.205ArgSer: 4.205 ± 0.037
4.928ArgThr: 4.928 ± 0.039
5.948ArgVal: 5.948 ± 0.042
1.571ArgTrp: 1.571 ± 0.024
2.003ArgTyr: 2.003 ± 0.023
0.001ArgXaa: 0.001 ± 0.0
Ser
6.941SerAla: 6.941 ± 0.043
0.418SerCys: 0.418 ± 0.012
2.576SerAsp: 2.576 ± 0.026
1.961SerGlu: 1.961 ± 0.023
1.545SerPhe: 1.545 ± 0.021
5.637SerGly: 5.637 ± 0.042
0.955SerHis: 0.955 ± 0.014
1.736SerIle: 1.736 ± 0.025
0.89SerLys: 0.89 ± 0.018
4.606SerLeu: 4.606 ± 0.042
1.053SerMet: 1.053 ± 0.014
0.937SerAsn: 0.937 ± 0.019
3.188SerPro: 3.188 ± 0.033
1.267SerGln: 1.267 ± 0.018
3.917SerArg: 3.917 ± 0.034
2.881SerSer: 2.881 ± 0.034
3.335SerThr: 3.335 ± 0.035
4.187SerVal: 4.187 ± 0.033
1.009SerTrp: 1.009 ± 0.019
1.26SerTyr: 1.26 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.156ThrAla: 9.156 ± 0.064
0.48ThrCys: 0.48 ± 0.013
3.743ThrAsp: 3.743 ± 0.035
2.913ThrGlu: 2.913 ± 0.028
1.831ThrPhe: 1.831 ± 0.026
6.797ThrGly: 6.797 ± 0.05
1.115ThrHis: 1.115 ± 0.016
2.369ThrIle: 2.369 ± 0.027
1.11ThrLys: 1.11 ± 0.023
5.821ThrLeu: 5.821 ± 0.041
1.1ThrMet: 1.1 ± 0.018
1.198ThrAsn: 1.198 ± 0.023
3.934ThrPro: 3.934 ± 0.04
1.373ThrGln: 1.373 ± 0.019
4.045ThrArg: 4.045 ± 0.036
3.218ThrSer: 3.218 ± 0.04
4.157ThrThr: 4.157 ± 0.048
6.418ThrVal: 6.418 ± 0.051
1.016ThrTrp: 1.016 ± 0.02
1.398ThrTyr: 1.398 ± 0.024
0.0ThrXaa: 0.0 ± 0.0
Val
12.175ValAla: 12.175 ± 0.069
0.688ValCys: 0.688 ± 0.013
5.279ValAsp: 5.279 ± 0.04
4.25ValGlu: 4.25 ± 0.039
2.316ValPhe: 2.316 ± 0.026
6.774ValGly: 6.774 ± 0.047
1.794ValHis: 1.794 ± 0.025
3.198ValIle: 3.198 ± 0.035
1.507ValLys: 1.507 ± 0.023
9.567ValLeu: 9.567 ± 0.064
1.218ValMet: 1.218 ± 0.021
1.759ValAsn: 1.759 ± 0.022
5.092ValPro: 5.092 ± 0.039
2.067ValGln: 2.067 ± 0.025
6.927ValArg: 6.927 ± 0.046
4.468ValSer: 4.468 ± 0.036
6.093ValThr: 6.093 ± 0.045
8.147ValVal: 8.147 ± 0.062
1.124ValTrp: 1.124 ± 0.019
1.55ValTyr: 1.55 ± 0.021
0.0ValXaa: 0.0 ± 0.0
Trp
1.769TrpAla: 1.769 ± 0.023
0.16TrpCys: 0.16 ± 0.008
0.787TrpAsp: 0.787 ± 0.016
0.604TrpGlu: 0.604 ± 0.013
0.478TrpPhe: 0.478 ± 0.011
0.982TrpGly: 0.982 ± 0.015
0.426TrpHis: 0.426 ± 0.012
0.606TrpIle: 0.606 ± 0.013
0.302TrpLys: 0.302 ± 0.009
1.935TrpLeu: 1.935 ± 0.025
0.296TrpMet: 0.296 ± 0.009
0.455TrpAsn: 0.455 ± 0.013
0.892TrpPro: 0.892 ± 0.017
0.758TrpGln: 0.758 ± 0.016
1.508TrpArg: 1.508 ± 0.021
1.087TrpSer: 1.087 ± 0.018
1.111TrpThr: 1.111 ± 0.02
1.08TrpVal: 1.08 ± 0.016
0.397TrpTrp: 0.397 ± 0.009
0.366TrpTyr: 0.366 ± 0.008
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.027
0.174TyrCys: 0.174 ± 0.007
1.431TyrAsp: 1.431 ± 0.026
1.019TyrGlu: 1.019 ± 0.016
0.656TyrPhe: 0.656 ± 0.014
2.118TyrGly: 2.118 ± 0.029
0.457TyrHis: 0.457 ± 0.012
0.452TyrIle: 0.452 ± 0.013
0.326TyrLys: 0.326 ± 0.011
2.379TyrLeu: 2.379 ± 0.03
0.205TyrMet: 0.205 ± 0.007
0.451TyrAsn: 0.451 ± 0.013
1.18TyrPro: 1.18 ± 0.018
0.724TyrGln: 0.724 ± 0.015
1.978TyrArg: 1.978 ± 0.021
0.99TyrSer: 0.99 ± 0.018
1.229TyrThr: 1.229 ± 0.023
1.723TyrVal: 1.723 ± 0.022
0.356TyrTrp: 0.356 ± 0.012
0.49TyrTyr: 0.49 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.001XaaGln: 0.001 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 11557 proteins (3748102 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski