Alicyclobacillus acidoterrestris (strain ATCC 49025 / DSM 3922 / CIP 106132 / NCIMB 13137 / GD3B)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Alicyclobacillus; Alicyclobacillus acidoterrestris

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0CVT4|T0CVT4_ALIAG AA_kinase domain-containing protein OS=Alicyclobacillus acidoterrestris (strain ATCC 49025 / DSM 3922 / CIP 106132 / NCIMB 13137 / GD3B) OX=1356854 GN=N007_16740 PE=4 SV=1
MM1 pKa = 7.43AAYY4 pKa = 10.21LIKK7 pKa = 10.54LYY9 pKa = 10.64VAAVSDD15 pKa = 4.21APTATGGTVTTTVSPVVTRR34 pKa = 11.84FHH36 pKa = 6.83APVSADD42 pKa = 3.48MIGTTDD48 pKa = 3.26TTIPAASFTDD58 pKa = 3.74DD59 pKa = 3.75AGNAVTTLPSPPTAGFFNVYY79 pKa = 10.07VNGVLQQSGLSTLSADD95 pKa = 3.92SLVLATTGITAGTPVVLEE113 pKa = 4.04VADD116 pKa = 4.81FSNTTSAITAEE127 pKa = 4.3PTISAPTITVMVV139 pKa = 4.33

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0BNK6|T0BNK6_ALIAG Uncharacterized protein OS=Alicyclobacillus acidoterrestris (strain ATCC 49025 / DSM 3922 / CIP 106132 / NCIMB 13137 / GD3B) OX=1356854 GN=N007_08635 PE=4 SV=1
MM1 pKa = 7.35LRR3 pKa = 11.84GAGAVPRR10 pKa = 11.84PPPLPRR16 pKa = 11.84AVGRR20 pKa = 11.84ASALGRR26 pKa = 11.84LTVAGGTRR34 pKa = 11.84IRR36 pKa = 4.02

Molecular weight: 3.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4102

0

4102

1149605

27

1925

280.3

31.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.353 ± 0.046

0.904 ± 0.013

5.243 ± 0.027

5.94 ± 0.043

3.867 ± 0.029

7.53 ± 0.036

2.469 ± 0.02

6.054 ± 0.03

3.976 ± 0.03

9.717 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.018

3.278 ± 0.025

4.253 ± 0.026

4.224 ± 0.031

5.98 ± 0.037

5.967 ± 0.035

5.822 ± 0.032

8.366 ± 0.038

1.336 ± 0.017

3.026 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski