Torque teno mini virus ALA22
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076V7U4|A0A076V7U4_9VIRU Uncharacterized protein OS=Torque teno mini virus ALA22 OX=1535290 GN=ALA22_gp1 PE=4 SV=1
MM1 pKa = 6.6 TTRR4 pKa = 11.84 IVPTKK9 pKa = 10.82 DD10 pKa = 3.38 SIRR13 pKa = 11.84 QKK15 pKa = 10.24 NLKK18 pKa = 8.25 WMNLLVHH25 pKa = 6.09 SHH27 pKa = 7.7 DD28 pKa = 4.19 IFCDD32 pKa = 3.54 CDD34 pKa = 3.86 SPLQHH39 pKa = 6.43 TLILICQQEE48 pKa = 4.3 PKK50 pKa = 10.12 IEE52 pKa = 4.15 LKK54 pKa = 10.6 PIEE57 pKa = 4.24 KK58 pKa = 10.39 DD59 pKa = 3.17 IIKK62 pKa = 10.25 KK63 pKa = 10.31 CLIGDD68 pKa = 3.75 TTEE71 pKa = 4.14 AAGDD75 pKa = 3.67 PAEE78 pKa = 5.05 DD79 pKa = 3.9 GVEE82 pKa = 3.98 EE83 pKa = 4.42 GLLDD87 pKa = 4.3 ALFKK91 pKa = 11.11 EE92 pKa = 4.88 DD93 pKa = 5.18 FGEE96 pKa = 4.18 EE97 pKa = 3.87 DD98 pKa = 3.24 TAGG101 pKa = 3.29
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.584
IPC2_protein 4.533
IPC_protein 4.444
Toseland 4.279
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.291
Rodwell 4.279
Grimsley 4.19
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.698
Thurlkill 4.291
EMBOSS 4.317
Sillero 4.558
Patrickios 3.961
IPC_peptide 4.406
IPC2_peptide 4.546
IPC2.peptide.svr19 4.499
Protein with the highest isoelectric point:
>tr|A0A076V365|A0A076V365_9VIRU Capsid protein OS=Torque teno mini virus ALA22 OX=1535290 GN=ALA22_gp2 PE=3 SV=1
MM1 pKa = 7.56 FFTLSLGEE9 pKa = 4.45 HH10 pKa = 6.4 HH11 pKa = 7.16 LLWPLSQNQTNNQNIPLPITSSKK34 pKa = 10.49 HH35 pKa = 3.73 LRR37 pKa = 11.84 CRR39 pKa = 11.84 VQHH42 pKa = 6.41 LPLNTSSTTSTKK54 pKa = 11.16 DD55 pKa = 3.2 EE56 pKa = 3.85 VHH58 pKa = 5.99 LQKK61 pKa = 11.09 KK62 pKa = 8.8 LLKK65 pKa = 10.12 EE66 pKa = 4.09 SQNTKK71 pKa = 10.26 RR72 pKa = 11.84 LNKK75 pKa = 10.12 LFSRR79 pKa = 11.84 LQNQLYY85 pKa = 8.78 PATQHH90 pKa = 6.1 PKK92 pKa = 9.95 KK93 pKa = 10.13 KK94 pKa = 9.61 HH95 pKa = 4.56 RR96 pKa = 11.84 HH97 pKa = 4.21 QKK99 pKa = 9.68 HH100 pKa = 4.16 RR101 pKa = 11.84 TRR103 pKa = 11.84 KK104 pKa = 9.36 KK105 pKa = 10.1 KK106 pKa = 9.84 KK107 pKa = 7.61 CHH109 pKa = 5.73 SRR111 pKa = 11.84 NSSSSSEE118 pKa = 4.13 SSKK121 pKa = 11.12 DD122 pKa = 3.43 SSEE125 pKa = 4.39 SEE127 pKa = 4.37 STNSSTDD134 pKa = 2.76
Molecular weight: 15.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.169
IPC2_protein 9.545
IPC_protein 9.56
Toseland 10.745
ProMoST 10.175
Dawson 10.804
Bjellqvist 10.365
Wikipedia 10.891
Rodwell 11.433
Grimsley 10.818
Solomon 10.847
Lehninger 10.847
Nozaki 10.716
DTASelect 10.365
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.716
Patrickios 11.169
IPC_peptide 10.862
IPC2_peptide 8.873
IPC2.peptide.svr19 8.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
909
101
674
303.0
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.63 ± 0.793
1.1 ± 0.877
4.4 ± 1.63
5.281 ± 1.211
4.07 ± 0.772
4.29 ± 0.974
2.97 ± 1.403
5.171 ± 1.282
8.361 ± 1.201
10.451 ± 0.536
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.76 ± 0.292
5.281 ± 0.855
5.831 ± 0.585
6.051 ± 0.544
6.051 ± 1.167
6.271 ± 3.066
10.451 ± 1.289
2.53 ± 0.286
1.98 ± 0.575
4.07 ± 1.887
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here