Streptococcus phage Javan400

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B4G1|A0A4D6B4G1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan400 OX=2548150 GN=Javan400_0038 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.75EE3 pKa = 3.77NNKK6 pKa = 10.35AGMMTLPIDD15 pKa = 3.76YY16 pKa = 10.53ANRR19 pKa = 11.84ALAKK23 pKa = 10.57EE24 pKa = 4.33EE25 pKa = 4.13ILDD28 pKa = 3.75EE29 pKa = 4.81LIEE32 pKa = 4.28RR33 pKa = 11.84EE34 pKa = 4.1IVEE37 pKa = 4.48VYY39 pKa = 10.11QLEE42 pKa = 4.75EE43 pKa = 3.81IAEE46 pKa = 4.36DD47 pKa = 4.49NPFLMNALNYY57 pKa = 8.9EE58 pKa = 4.26VQEE61 pKa = 4.18

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B1R6|A0A4D6B1R6_9CAUD Replication protein OS=Streptococcus phage Javan400 OX=2548150 GN=Javan400_0037 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 5.08DD3 pKa = 4.04VITISRR9 pKa = 11.84SEE11 pKa = 4.26LKK13 pKa = 10.75ALIAEE18 pKa = 4.45EE19 pKa = 4.04VAIQHH24 pKa = 5.99RR25 pKa = 11.84QVDD28 pKa = 3.73WVSIKK33 pKa = 10.25EE34 pKa = 4.05LQTITGWGRR43 pKa = 11.84TTLEE47 pKa = 3.44NWRR50 pKa = 11.84DD51 pKa = 3.47QGKK54 pKa = 8.89FRR56 pKa = 11.84YY57 pKa = 7.89HH58 pKa = 5.58QKK60 pKa = 10.54VKK62 pKa = 10.37GGKK65 pKa = 7.65YY66 pKa = 8.92TYY68 pKa = 10.55DD69 pKa = 3.43LQDD72 pKa = 2.97VQRR75 pKa = 11.84FLRR78 pKa = 11.84SMNKK82 pKa = 9.73

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10663

39

1452

205.1

23.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.377 ± 0.497

0.61 ± 0.112

6.452 ± 0.213

7.793 ± 0.457

4.305 ± 0.269

5.721 ± 0.381

1.36 ± 0.176

7.24 ± 0.251

8.937 ± 0.405

7.943 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.206

6.293 ± 0.299

2.56 ± 0.18

4.014 ± 0.197

3.367 ± 0.277

6.002 ± 0.289

6.912 ± 0.602

6.462 ± 0.287

1.313 ± 0.153

3.873 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski