Amino acid dipepetide frequency for Streptococcus phage Javan400

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.125AlaAla: 1.125 ± 0.439
0.657AlaCys: 0.657 ± 0.238
3.658AlaAsp: 3.658 ± 0.533
5.252AlaGlu: 5.252 ± 0.621
2.532AlaPhe: 2.532 ± 0.441
3.752AlaGly: 3.752 ± 0.812
1.313AlaHis: 1.313 ± 0.361
5.346AlaIle: 5.346 ± 0.949
7.034AlaLys: 7.034 ± 0.592
5.534AlaLeu: 5.534 ± 0.995
2.908AlaMet: 2.908 ± 0.549
3.376AlaAsn: 3.376 ± 0.711
1.594AlaPro: 1.594 ± 0.442
2.908AlaGln: 2.908 ± 0.539
1.876AlaArg: 1.876 ± 0.438
4.033AlaSer: 4.033 ± 0.552
3.564AlaThr: 3.564 ± 0.642
3.47AlaVal: 3.47 ± 0.499
1.313AlaTrp: 1.313 ± 0.356
2.532AlaTyr: 2.532 ± 0.526
0.0AlaXaa: 0.0 ± 0.0
Cys
0.188CysAla: 0.188 ± 0.198
0.094CysCys: 0.094 ± 0.099
0.281CysAsp: 0.281 ± 0.16
0.188CysGlu: 0.188 ± 0.122
0.188CysPhe: 0.188 ± 0.166
0.657CysGly: 0.657 ± 0.212
0.188CysHis: 0.188 ± 0.146
0.469CysIle: 0.469 ± 0.245
0.281CysLys: 0.281 ± 0.157
0.188CysLeu: 0.188 ± 0.124
0.188CysMet: 0.188 ± 0.149
0.375CysAsn: 0.375 ± 0.174
0.188CysPro: 0.188 ± 0.129
0.281CysGln: 0.281 ± 0.148
0.094CysArg: 0.094 ± 0.093
0.563CysSer: 0.563 ± 0.23
0.469CysThr: 0.469 ± 0.218
0.375CysVal: 0.375 ± 0.2
0.188CysTrp: 0.188 ± 0.131
0.657CysTyr: 0.657 ± 0.227
0.0CysXaa: 0.0 ± 0.0
Asp
2.72AspAla: 2.72 ± 0.63
0.375AspCys: 0.375 ± 0.261
3.47AspAsp: 3.47 ± 0.595
5.252AspGlu: 5.252 ± 0.788
3.47AspPhe: 3.47 ± 0.76
3.752AspGly: 3.752 ± 0.512
0.563AspHis: 0.563 ± 0.219
5.346AspIle: 5.346 ± 0.593
5.534AspLys: 5.534 ± 0.816
6.19AspLeu: 6.19 ± 0.665
0.844AspMet: 0.844 ± 0.288
4.408AspAsn: 4.408 ± 0.532
1.688AspPro: 1.688 ± 0.369
1.125AspGln: 1.125 ± 0.339
1.876AspArg: 1.876 ± 0.395
4.127AspSer: 4.127 ± 0.842
3.189AspThr: 3.189 ± 0.431
5.534AspVal: 5.534 ± 0.586
1.501AspTrp: 1.501 ± 0.285
3.564AspTyr: 3.564 ± 0.623
0.0AspXaa: 0.0 ± 0.0
Glu
4.971GluAla: 4.971 ± 0.978
0.563GluCys: 0.563 ± 0.305
3.752GluAsp: 3.752 ± 0.642
6.378GluGlu: 6.378 ± 0.734
3.752GluPhe: 3.752 ± 0.633
3.189GluGly: 3.189 ± 0.588
0.844GluHis: 0.844 ± 0.32
6.472GluIle: 6.472 ± 0.886
7.128GluLys: 7.128 ± 1.105
8.066GluLeu: 8.066 ± 1.033
2.063GluMet: 2.063 ± 0.581
3.564GluAsn: 3.564 ± 0.46
1.407GluPro: 1.407 ± 0.412
4.221GluGln: 4.221 ± 0.629
3.095GluArg: 3.095 ± 0.536
3.658GluSer: 3.658 ± 0.701
4.877GluThr: 4.877 ± 0.787
6.284GluVal: 6.284 ± 0.763
0.657GluTrp: 0.657 ± 0.258
2.908GluTyr: 2.908 ± 0.506
0.0GluXaa: 0.0 ± 0.0
Phe
2.626PheAla: 2.626 ± 0.48
0.094PheCys: 0.094 ± 0.099
3.939PheAsp: 3.939 ± 0.617
3.845PheGlu: 3.845 ± 0.658
1.407PhePhe: 1.407 ± 0.403
2.814PheGly: 2.814 ± 0.579
0.375PheHis: 0.375 ± 0.159
3.001PheIle: 3.001 ± 0.466
4.033PheLys: 4.033 ± 0.67
2.063PheLeu: 2.063 ± 0.571
1.219PheMet: 1.219 ± 0.347
3.658PheAsn: 3.658 ± 0.642
1.219PhePro: 1.219 ± 0.401
1.594PheGln: 1.594 ± 0.339
1.782PheArg: 1.782 ± 0.37
2.251PheSer: 2.251 ± 0.423
3.564PheThr: 3.564 ± 0.455
1.876PheVal: 1.876 ± 0.32
0.563PheTrp: 0.563 ± 0.31
1.125PheTyr: 1.125 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
3.939GlyAla: 3.939 ± 0.952
0.469GlyCys: 0.469 ± 0.244
3.001GlyAsp: 3.001 ± 0.423
3.376GlyGlu: 3.376 ± 0.551
3.47GlyPhe: 3.47 ± 0.451
3.564GlyGly: 3.564 ± 0.628
0.563GlyHis: 0.563 ± 0.227
3.658GlyIle: 3.658 ± 0.792
5.627GlyLys: 5.627 ± 0.848
4.971GlyLeu: 4.971 ± 0.78
1.501GlyMet: 1.501 ± 0.387
2.626GlyAsn: 2.626 ± 0.377
0.094GlyPro: 0.094 ± 0.093
2.251GlyGln: 2.251 ± 0.388
2.157GlyArg: 2.157 ± 0.517
3.658GlySer: 3.658 ± 0.686
4.408GlyThr: 4.408 ± 0.536
4.408GlyVal: 4.408 ± 0.553
0.938GlyTrp: 0.938 ± 0.287
2.532GlyTyr: 2.532 ± 0.526
0.0GlyXaa: 0.0 ± 0.0
His
0.657HisAla: 0.657 ± 0.294
0.188HisCys: 0.188 ± 0.125
1.313HisAsp: 1.313 ± 0.405
1.032HisGlu: 1.032 ± 0.351
0.657HisPhe: 0.657 ± 0.282
0.844HisGly: 0.844 ± 0.288
0.188HisHis: 0.188 ± 0.11
1.501HisIle: 1.501 ± 0.31
0.657HisLys: 0.657 ± 0.205
1.313HisLeu: 1.313 ± 0.291
0.281HisMet: 0.281 ± 0.177
0.469HisAsn: 0.469 ± 0.219
0.281HisPro: 0.281 ± 0.216
0.281HisGln: 0.281 ± 0.135
0.75HisArg: 0.75 ± 0.247
0.75HisSer: 0.75 ± 0.252
0.469HisThr: 0.469 ± 0.169
1.407HisVal: 1.407 ± 0.437
0.094HisTrp: 0.094 ± 0.099
0.469HisTyr: 0.469 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.314IleAla: 4.314 ± 0.719
0.75IleCys: 0.75 ± 0.306
7.222IleAsp: 7.222 ± 0.801
5.44IleGlu: 5.44 ± 0.772
3.189IlePhe: 3.189 ± 0.435
3.752IleGly: 3.752 ± 1.026
0.938IleHis: 0.938 ± 0.275
4.221IleIle: 4.221 ± 0.521
6.659IleLys: 6.659 ± 0.783
4.502IleLeu: 4.502 ± 0.633
1.97IleMet: 1.97 ± 0.416
5.534IleAsn: 5.534 ± 0.472
2.251IlePro: 2.251 ± 0.442
2.814IleGln: 2.814 ± 0.471
1.876IleArg: 1.876 ± 0.462
4.596IleSer: 4.596 ± 0.624
4.971IleThr: 4.971 ± 0.824
5.065IleVal: 5.065 ± 0.639
0.469IleTrp: 0.469 ± 0.234
1.876IleTyr: 1.876 ± 0.344
0.0IleXaa: 0.0 ± 0.0
Lys
6.472LysAla: 6.472 ± 0.639
0.094LysCys: 0.094 ± 0.103
4.596LysAsp: 4.596 ± 0.517
9.285LysGlu: 9.285 ± 1.398
3.001LysPhe: 3.001 ± 0.466
5.159LysGly: 5.159 ± 0.637
1.407LysHis: 1.407 ± 0.469
6.284LysIle: 6.284 ± 0.702
7.503LysLys: 7.503 ± 1.439
7.409LysLeu: 7.409 ± 0.863
1.97LysMet: 1.97 ± 0.394
4.783LysAsn: 4.783 ± 0.75
2.251LysPro: 2.251 ± 0.413
4.596LysGln: 4.596 ± 0.609
2.72LysArg: 2.72 ± 0.603
5.346LysSer: 5.346 ± 0.673
7.128LysThr: 7.128 ± 0.75
4.877LysVal: 4.877 ± 0.607
1.125LysTrp: 1.125 ± 0.321
3.376LysTyr: 3.376 ± 0.452
0.0LysXaa: 0.0 ± 0.0
Leu
5.534LeuAla: 5.534 ± 0.9
0.469LeuCys: 0.469 ± 0.181
4.971LeuAsp: 4.971 ± 0.492
6.565LeuGlu: 6.565 ± 0.773
3.283LeuPhe: 3.283 ± 0.569
4.69LeuGly: 4.69 ± 0.663
0.75LeuHis: 0.75 ± 0.31
5.909LeuIle: 5.909 ± 0.61
8.16LeuLys: 8.16 ± 1.267
5.159LeuLeu: 5.159 ± 0.765
2.345LeuMet: 2.345 ± 0.466
4.971LeuAsn: 4.971 ± 0.726
2.908LeuPro: 2.908 ± 0.567
4.033LeuGln: 4.033 ± 0.739
2.157LeuArg: 2.157 ± 0.632
5.065LeuSer: 5.065 ± 0.579
4.971LeuThr: 4.971 ± 0.639
3.845LeuVal: 3.845 ± 0.714
0.75LeuTrp: 0.75 ± 0.197
2.908LeuTyr: 2.908 ± 0.521
0.0LeuXaa: 0.0 ± 0.0
Met
1.407MetAla: 1.407 ± 0.328
0.188MetCys: 0.188 ± 0.127
1.594MetAsp: 1.594 ± 0.33
1.782MetGlu: 1.782 ± 0.435
0.375MetPhe: 0.375 ± 0.204
1.219MetGly: 1.219 ± 0.411
0.188MetHis: 0.188 ± 0.135
1.782MetIle: 1.782 ± 0.408
2.439MetLys: 2.439 ± 0.408
1.313MetLeu: 1.313 ± 0.301
0.563MetMet: 0.563 ± 0.198
2.439MetAsn: 2.439 ± 0.562
0.844MetPro: 0.844 ± 0.31
1.125MetGln: 1.125 ± 0.222
0.75MetArg: 0.75 ± 0.284
1.782MetSer: 1.782 ± 0.539
1.97MetThr: 1.97 ± 0.46
1.97MetVal: 1.97 ± 0.449
0.281MetTrp: 0.281 ± 0.15
0.657MetTyr: 0.657 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
4.502AsnAla: 4.502 ± 0.82
0.375AsnCys: 0.375 ± 0.212
4.127AsnAsp: 4.127 ± 0.652
3.283AsnGlu: 3.283 ± 0.399
2.532AsnPhe: 2.532 ± 0.476
6.19AsnGly: 6.19 ± 0.908
1.594AsnHis: 1.594 ± 0.334
4.127AsnIle: 4.127 ± 0.591
5.346AsnLys: 5.346 ± 0.573
4.502AsnLeu: 4.502 ± 0.469
1.407AsnMet: 1.407 ± 0.388
4.783AsnAsn: 4.783 ± 0.794
1.876AsnPro: 1.876 ± 0.33
2.157AsnGln: 2.157 ± 0.455
2.157AsnArg: 2.157 ± 0.448
3.095AsnSer: 3.095 ± 0.435
3.658AsnThr: 3.658 ± 0.617
3.845AsnVal: 3.845 ± 0.561
0.938AsnTrp: 0.938 ± 0.334
2.439AsnTyr: 2.439 ± 0.425
0.0AsnXaa: 0.0 ± 0.0
Pro
1.782ProAla: 1.782 ± 0.328
0.188ProCys: 0.188 ± 0.133
1.501ProAsp: 1.501 ± 0.388
1.594ProGlu: 1.594 ± 0.329
1.219ProPhe: 1.219 ± 0.35
0.469ProGly: 0.469 ± 0.313
0.469ProHis: 0.469 ± 0.196
2.908ProIle: 2.908 ± 0.45
2.532ProLys: 2.532 ± 0.619
1.594ProLeu: 1.594 ± 0.299
0.281ProMet: 0.281 ± 0.143
1.501ProAsn: 1.501 ± 0.364
0.563ProPro: 0.563 ± 0.293
1.876ProGln: 1.876 ± 0.409
1.313ProArg: 1.313 ± 0.353
1.688ProSer: 1.688 ± 0.459
2.063ProThr: 2.063 ± 0.483
0.844ProVal: 0.844 ± 0.275
0.188ProTrp: 0.188 ± 0.133
1.032ProTyr: 1.032 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
3.845GlnAla: 3.845 ± 0.744
0.188GlnCys: 0.188 ± 0.131
1.594GlnAsp: 1.594 ± 0.37
2.439GlnGlu: 2.439 ± 0.793
1.688GlnPhe: 1.688 ± 0.375
1.688GlnGly: 1.688 ± 0.365
0.75GlnHis: 0.75 ± 0.242
2.626GlnIle: 2.626 ± 0.468
2.063GlnLys: 2.063 ± 0.469
3.752GlnLeu: 3.752 ± 0.561
0.657GlnMet: 0.657 ± 0.252
2.157GlnAsn: 2.157 ± 0.504
0.657GlnPro: 0.657 ± 0.237
1.219GlnGln: 1.219 ± 0.314
1.407GlnArg: 1.407 ± 0.311
3.376GlnSer: 3.376 ± 0.625
4.596GlnThr: 4.596 ± 1.12
3.47GlnVal: 3.47 ± 0.638
0.75GlnTrp: 0.75 ± 0.366
1.219GlnTyr: 1.219 ± 0.368
0.0GlnXaa: 0.0 ± 0.0
Arg
2.626ArgAla: 2.626 ± 0.36
0.281ArgCys: 0.281 ± 0.156
1.97ArgAsp: 1.97 ± 0.427
3.001ArgGlu: 3.001 ± 0.47
1.501ArgPhe: 1.501 ± 0.334
1.501ArgGly: 1.501 ± 0.337
0.375ArgHis: 0.375 ± 0.18
2.439ArgIle: 2.439 ± 0.649
2.814ArgLys: 2.814 ± 0.518
3.658ArgLeu: 3.658 ± 0.571
0.188ArgMet: 0.188 ± 0.126
1.876ArgAsn: 1.876 ± 0.412
0.938ArgPro: 0.938 ± 0.276
1.501ArgGln: 1.501 ± 0.316
1.407ArgArg: 1.407 ± 0.442
1.688ArgSer: 1.688 ± 0.363
1.688ArgThr: 1.688 ± 0.392
1.782ArgVal: 1.782 ± 0.43
0.563ArgTrp: 0.563 ± 0.194
1.876ArgTyr: 1.876 ± 0.502
0.0ArgXaa: 0.0 ± 0.0
Ser
4.127SerAla: 4.127 ± 0.865
0.094SerCys: 0.094 ± 0.077
4.877SerAsp: 4.877 ± 0.757
4.783SerGlu: 4.783 ± 0.532
1.594SerPhe: 1.594 ± 0.32
4.502SerGly: 4.502 ± 0.65
0.563SerHis: 0.563 ± 0.193
3.564SerIle: 3.564 ± 0.527
4.596SerLys: 4.596 ± 0.664
4.783SerLeu: 4.783 ± 0.718
1.501SerMet: 1.501 ± 0.324
3.939SerAsn: 3.939 ± 0.481
1.688SerPro: 1.688 ± 0.453
2.063SerGln: 2.063 ± 0.426
2.157SerArg: 2.157 ± 0.492
3.845SerSer: 3.845 ± 0.679
5.065SerThr: 5.065 ± 0.739
3.939SerVal: 3.939 ± 0.576
0.563SerTrp: 0.563 ± 0.242
1.782SerTyr: 1.782 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
4.69ThrAla: 4.69 ± 0.927
0.188ThrCys: 0.188 ± 0.114
4.502ThrAsp: 4.502 ± 0.706
4.221ThrGlu: 4.221 ± 0.542
3.658ThrPhe: 3.658 ± 0.794
4.127ThrGly: 4.127 ± 0.701
1.125ThrHis: 1.125 ± 0.4
6.096ThrIle: 6.096 ± 0.66
6.003ThrLys: 6.003 ± 0.759
3.845ThrLeu: 3.845 ± 0.61
1.313ThrMet: 1.313 ± 0.332
5.346ThrAsn: 5.346 ± 0.811
1.876ThrPro: 1.876 ± 0.467
2.251ThrGln: 2.251 ± 0.5
1.782ThrArg: 1.782 ± 0.37
3.658ThrSer: 3.658 ± 0.643
5.159ThrThr: 5.159 ± 1.318
6.003ThrVal: 6.003 ± 1.028
0.75ThrTrp: 0.75 ± 0.226
2.532ThrTyr: 2.532 ± 0.56
0.0ThrXaa: 0.0 ± 0.0
Val
5.159ValAla: 5.159 ± 0.758
0.094ValCys: 0.094 ± 0.096
4.877ValAsp: 4.877 ± 0.687
5.44ValGlu: 5.44 ± 0.642
1.876ValPhe: 1.876 ± 0.454
2.251ValGly: 2.251 ± 0.446
0.563ValHis: 0.563 ± 0.212
3.752ValIle: 3.752 ± 0.609
7.128ValLys: 7.128 ± 0.751
5.534ValLeu: 5.534 ± 0.686
1.97ValMet: 1.97 ± 0.521
3.376ValAsn: 3.376 ± 0.842
2.157ValPro: 2.157 ± 0.593
1.876ValGln: 1.876 ± 0.425
2.439ValArg: 2.439 ± 0.475
3.564ValSer: 3.564 ± 0.521
4.783ValThr: 4.783 ± 0.779
3.752ValVal: 3.752 ± 0.681
1.032ValTrp: 1.032 ± 0.31
3.001ValTyr: 3.001 ± 0.636
0.0ValXaa: 0.0 ± 0.0
Trp
0.563TrpAla: 0.563 ± 0.292
0.375TrpCys: 0.375 ± 0.228
0.563TrpAsp: 0.563 ± 0.223
1.407TrpGlu: 1.407 ± 0.44
0.938TrpPhe: 0.938 ± 0.346
0.938TrpGly: 0.938 ± 0.222
0.188TrpHis: 0.188 ± 0.132
0.469TrpIle: 0.469 ± 0.226
0.844TrpLys: 0.844 ± 0.308
1.688TrpLeu: 1.688 ± 0.401
0.75TrpMet: 0.75 ± 0.307
0.938TrpAsn: 0.938 ± 0.227
0.188TrpPro: 0.188 ± 0.171
0.375TrpGln: 0.375 ± 0.179
0.657TrpArg: 0.657 ± 0.225
1.125TrpSer: 1.125 ± 0.292
0.375TrpThr: 0.375 ± 0.199
0.375TrpVal: 0.375 ± 0.166
0.094TrpTrp: 0.094 ± 0.085
0.281TrpTyr: 0.281 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.532TyrAla: 2.532 ± 0.583
0.375TyrCys: 0.375 ± 0.179
2.72TyrAsp: 2.72 ± 0.398
3.376TyrGlu: 3.376 ± 0.686
2.72TyrPhe: 2.72 ± 0.487
2.157TyrGly: 2.157 ± 0.483
0.657TyrHis: 0.657 ± 0.18
2.439TyrIle: 2.439 ± 0.613
3.001TyrLys: 3.001 ± 0.593
3.376TyrLeu: 3.376 ± 0.617
0.657TyrMet: 0.657 ± 0.289
2.814TyrAsn: 2.814 ± 0.539
0.938TyrPro: 0.938 ± 0.341
1.594TyrGln: 1.594 ± 0.377
1.219TyrArg: 1.219 ± 0.405
2.157TyrSer: 2.157 ± 0.516
2.157TyrThr: 2.157 ± 0.444
1.501TyrVal: 1.501 ± 0.288
0.375TyrTrp: 0.375 ± 0.157
1.97TyrTyr: 1.97 ± 0.486
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (10663 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski