Gordonia Phage Jablanski
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5G1M6|A0A7D5G1M6_9CAUD Uncharacterized protein OS=Gordonia Phage Jablanski OX=2743982 GN=50 PE=4 SV=1
MM1 pKa = 6.83 TTPPPPPMFTLPEE14 pKa = 4.24 YY15 pKa = 10.39 PGVTYY20 pKa = 9.85 TVLTGSGGVPGLPSNAVRR38 pKa = 11.84 IVGTAVNPWYY48 pKa = 10.23 EE49 pKa = 3.52 PGYY52 pKa = 11.14 NYY54 pKa = 10.89 GQDD57 pKa = 3.41 PNGFTEE63 pKa = 5.2 ITDD66 pKa = 2.91 PWKK69 pKa = 10.68 RR70 pKa = 11.84 HH71 pKa = 4.27 TEE73 pKa = 4.0 QIEE76 pKa = 4.19 VTNTGFLAPTAPVPAEE92 pKa = 4.4 PPPSSPATEE101 pKa = 4.6 PEE103 pKa = 4.12 PEE105 pKa = 4.08 PTPEE109 pKa = 3.89 EE110 pKa = 4.26 VV111 pKa = 3.16
Molecular weight: 11.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.911
IPC2_protein 4.164
IPC_protein 3.973
Toseland 3.846
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.821
Grimsley 3.77
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.062
Thurlkill 3.859
EMBOSS 3.77
Sillero 4.075
Patrickios 0.693
IPC_peptide 3.897
IPC2_peptide 4.075
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A7D5JUM2|A0A7D5JUM2_9CAUD Uncharacterized protein OS=Gordonia Phage Jablanski OX=2743982 GN=32 PE=4 SV=1
MM1 pKa = 7.75 SVRR4 pKa = 11.84 LKK6 pKa = 10.91 FNIDD10 pKa = 2.68 GFYY13 pKa = 10.88 DD14 pKa = 3.72 LRR16 pKa = 11.84 RR17 pKa = 11.84 DD18 pKa = 3.66 PGIVGQEE25 pKa = 3.64 EE26 pKa = 4.98 AIAQQICDD34 pKa = 3.57 RR35 pKa = 11.84 ANSIGKK41 pKa = 8.2 GTYY44 pKa = 10.12 AVGSRR49 pKa = 11.84 QGRR52 pKa = 11.84 KK53 pKa = 9.29 APQGRR58 pKa = 11.84 WRR60 pKa = 11.84 TTVVTADD67 pKa = 3.27 ARR69 pKa = 11.84 AMANNARR76 pKa = 11.84 NNTLIRR82 pKa = 11.84 AMEE85 pKa = 3.98
Molecular weight: 9.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.56
IPC_protein 10.526
Toseland 10.613
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.774
Solomon 10.862
Lehninger 10.833
Nozaki 10.599
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.862
IPC2_peptide 9.56
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
17204
47
1878
209.8
22.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.701 ± 0.536
0.965 ± 0.164
6.934 ± 0.273
5.673 ± 0.247
2.738 ± 0.131
8.55 ± 0.465
2.052 ± 0.218
4.644 ± 0.183
3.313 ± 0.206
7.673 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.023 ± 0.121
2.953 ± 0.192
5.574 ± 0.326
3.784 ± 0.383
7.719 ± 0.405
5.743 ± 0.252
6.853 ± 0.28
6.952 ± 0.252
1.994 ± 0.153
2.162 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here