Legionella rubrilucens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0XXK9|A0A0W0XXK9_9GAMM 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Legionella rubrilucens OX=458 GN=Lrub_1395 PE=3 SV=1
MM1 pKa = 7.43AAINAIPSSTTNVYY15 pKa = 10.3FSVSAADD22 pKa = 4.05KK23 pKa = 10.19VAALIAEE30 pKa = 4.38EE31 pKa = 5.1DD32 pKa = 3.86NPSLNLRR39 pKa = 11.84VYY41 pKa = 9.08ITGGGCSGFQYY52 pKa = 10.95GFTFDD57 pKa = 3.97EE58 pKa = 5.28TIQDD62 pKa = 3.74DD63 pKa = 4.18DD64 pKa = 4.28TVVVQTCSDD73 pKa = 3.56GQSTVKK79 pKa = 10.77LLIDD83 pKa = 3.8SMSHH87 pKa = 4.66QFLNEE92 pKa = 3.59AEE94 pKa = 3.86IDD96 pKa = 4.03YY97 pKa = 10.75VQGIQGEE104 pKa = 4.42QFVIRR109 pKa = 11.84NPNAKK114 pKa = 6.55TTCGCGSSFSMDD126 pKa = 3.49DD127 pKa = 3.28EE128 pKa = 4.47

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0XRK1|A0A0W0XRK1_9GAMM ABC_trans_aux domain-containing protein OS=Legionella rubrilucens OX=458 GN=Lrub_1914 PE=4 SV=1
MM1 pKa = 7.41HH2 pKa = 7.78EE3 pKa = 4.3SRR5 pKa = 11.84KK6 pKa = 10.28KK7 pKa = 10.53LIEE10 pKa = 4.23QIAHH14 pKa = 5.69QEE16 pKa = 3.64AALYY20 pKa = 9.97RR21 pKa = 11.84CYY23 pKa = 11.3DD24 pKa = 3.01KK25 pKa = 11.47VLAHH29 pKa = 6.3KK30 pKa = 10.27QYY32 pKa = 10.51LSSVIEE38 pKa = 4.11RR39 pKa = 11.84QEE41 pKa = 3.88SLIVLLVIPLLWGWRR56 pKa = 11.84QGRR59 pKa = 11.84VHH61 pKa = 6.56QGFRR65 pKa = 11.84QLLRR69 pKa = 11.84LTMSTLLVQAAALFRR84 pKa = 11.84RR85 pKa = 11.84KK86 pKa = 9.28LVQVARR92 pKa = 11.84RR93 pKa = 11.84SLVYY97 pKa = 9.74TPPDD101 pKa = 3.13VGKK104 pKa = 9.44PP105 pKa = 3.38

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2847

0

2847

955316

50

3874

335.6

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.64 ± 0.052

1.174 ± 0.015

5.036 ± 0.034

5.832 ± 0.04

4.256 ± 0.034

6.253 ± 0.047

2.694 ± 0.023

6.35 ± 0.035

5.484 ± 0.044

11.412 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.02

4.227 ± 0.034

4.386 ± 0.03

4.859 ± 0.035

4.899 ± 0.037

6.126 ± 0.032

5.24 ± 0.032

6.233 ± 0.039

1.183 ± 0.018

3.271 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski