Vibrio aerogenes CECT 7868
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4565 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5XSE5|A0A1M5XSE5_9VIBR Uncharacterized protein OS=Vibrio aerogenes CECT 7868 OX=1216006 GN=VA7868_01292 PE=4 SV=1
MM1 pKa = 7.48 IPVDD5 pKa = 3.62 SKK7 pKa = 10.17 TYY9 pKa = 9.68 HH10 pKa = 6.73 LLYY13 pKa = 10.23 TDD15 pKa = 3.92 EE16 pKa = 5.3 FGDD19 pKa = 4.71 DD20 pKa = 3.37 VGLDD24 pKa = 3.77 EE25 pKa = 5.4 VQLLDD30 pKa = 3.94 PPLKK34 pKa = 10.53 EE35 pKa = 5.09 RR36 pKa = 11.84 IPDD39 pKa = 4.13 LLALLDD45 pKa = 4.6 SPDD48 pKa = 3.56 QFISYY53 pKa = 10.09 QAMLILSAWGNDD65 pKa = 2.98 SGIYY69 pKa = 10.28 FLDD72 pKa = 4.73 SIIDD76 pKa = 3.56 DD77 pKa = 4.19 EE78 pKa = 5.28 KK79 pKa = 9.52 IHH81 pKa = 6.32 EE82 pKa = 4.71 SGFEE86 pKa = 3.69 LHH88 pKa = 7.4 RR89 pKa = 11.84 ITDD92 pKa = 3.44 EE93 pKa = 4.42 DD94 pKa = 3.57 NVYY97 pKa = 11.23 DD98 pKa = 4.04 EE99 pKa = 5.26 LAYY102 pKa = 9.44 ATYY105 pKa = 10.43 FF106 pKa = 3.47
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.516
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.859
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A1M5ZHA7|A0A1M5ZHA7_9VIBR tRNA modification GTPase MnmE OS=Vibrio aerogenes CECT 7868 OX=1216006 GN=mnmE PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4565
0
4565
1492859
29
4322
327.0
36.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.361 ± 0.034
1.168 ± 0.014
5.458 ± 0.026
6.023 ± 0.035
4.056 ± 0.025
6.864 ± 0.035
2.592 ± 0.016
6.398 ± 0.03
4.999 ± 0.034
10.126 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.684 ± 0.017
3.904 ± 0.022
4.122 ± 0.026
4.966 ± 0.033
4.807 ± 0.028
6.749 ± 0.034
5.468 ± 0.032
6.793 ± 0.031
1.254 ± 0.014
3.21 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here