Marivirga lumbricoides
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3452 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4DHK9|A0A2T4DHK9_9BACT Uncharacterized protein OS=Marivirga lumbricoides OX=1046115 GN=C9994_13130 PE=4 SV=1
QQ1 pKa = 8.12 DD2 pKa = 5.71 LLPQHH7 pKa = 6.49 NLPLHH12 pKa = 5.73 YY13 pKa = 10.6 LLLPQTTAFNVTVTDD28 pKa = 3.98 GAGTEE33 pKa = 4.53 LSFSPVTVSRR43 pKa = 11.84 TAPAIIPLSSGAGSPKK59 pKa = 10.41 GPNTKK64 pKa = 10.14 FLVDD68 pKa = 4.38 DD69 pKa = 4.06 SQLATVQTDD78 pKa = 2.9 EE79 pKa = 5.02 GLILTANKK87 pKa = 10.58 AFFTSIRR94 pKa = 11.84 ISEE97 pKa = 4.25 SAQAGFLTSKK107 pKa = 9.15 GTAGFGKK114 pKa = 9.85 EE115 pKa = 4.03 FRR117 pKa = 11.84 TGHH120 pKa = 5.13 IWNTTGAAGRR130 pKa = 11.84 KK131 pKa = 8.05 AHH133 pKa = 6.05 VFSFMATEE141 pKa = 4.48 DD142 pKa = 3.5 ATTVTISDD150 pKa = 4.16 FGAVDD155 pKa = 3.95 FEE157 pKa = 5.08 NISEE161 pKa = 4.27 SGDD164 pKa = 3.11 ITVSLDD170 pKa = 3.24 AGDD173 pKa = 4.51 SYY175 pKa = 12.07 VMAAFVDD182 pKa = 4.44 KK183 pKa = 10.86 PSTDD187 pKa = 2.94 NLNNVNGTRR196 pKa = 11.84 ISSNKK201 pKa = 9.84 NIVVNSGSWLGGSPGGTDD219 pKa = 3.18 EE220 pKa = 5.18 GRR222 pKa = 11.84 DD223 pKa = 3.18 IGLDD227 pKa = 3.12 QIAPIEE233 pKa = 4.22 QTGFEE238 pKa = 4.39 YY239 pKa = 10.47 ILVKK243 pKa = 10.6 GQGIASEE250 pKa = 4.12 NVIVVAHH257 pKa = 6.52 TDD259 pKa = 3.2 GTDD262 pKa = 2.68 IFLNGASSPANASPLNAGEE281 pKa = 3.91 FHH283 pKa = 7.31 RR284 pKa = 11.84 FTASDD289 pKa = 3.65 YY290 pKa = 10.81 SANEE294 pKa = 3.53 NMYY297 pKa = 8.64 ITSNQPVYY305 pKa = 10.73 VYY307 pKa = 10.6 QGLNGANSTNEE318 pKa = 3.75 RR319 pKa = 11.84 QLGMNYY325 pKa = 8.5 MPPIICLGGTNVDD338 pKa = 3.58 IPNINQLGNPVIQIIAEE355 pKa = 4.11 AGEE358 pKa = 4.24 QVTITDD364 pKa = 3.79 GSGTTDD370 pKa = 3.07 VTSSARR376 pKa = 11.84 AVTGNSNYY384 pKa = 7.83 VTYY387 pKa = 11.03 KK388 pKa = 10.04 LAGYY392 pKa = 7.32 TGNVTVEE399 pKa = 4.09 SPRR402 pKa = 11.84 PIRR405 pKa = 11.84 VSLTIEE411 pKa = 3.86 SGNIGGAGFFSGFTTSPVIEE431 pKa = 4.49 TPSGYY436 pKa = 10.34 DD437 pKa = 3.12 ATSCIPDD444 pKa = 3.89 NLPVTLTATGFDD456 pKa = 3.49 SYY458 pKa = 10.23 QWYY461 pKa = 10.03 RR462 pKa = 11.84 DD463 pKa = 3.82 GIILTGEE470 pKa = 4.13 TNASVSVDD478 pKa = 3.06 KK479 pKa = 11.11 PGVYY483 pKa = 8.66 TAAGTIAGCVSSEE496 pKa = 3.7 QSFPLSVTLCPGDD509 pKa = 3.91 LGVAKK514 pKa = 10.53 DD515 pKa = 3.94 VVSVTNVSGDD525 pKa = 3.42 LFDD528 pKa = 4.62 VVFDD532 pKa = 5.49 LIVTNYY538 pKa = 10.12 SPSNPAPNMQLIDD551 pKa = 5.68 DD552 pKa = 4.41 ITDD555 pKa = 4.19 GLPSGATASIQSAPTVVNGSFTTGGISSSYY585 pKa = 11.04 NGTSDD590 pKa = 3.19 IALLTTSLSGADD602 pKa = 3.66 TEE604 pKa = 4.5 LAAASTVTVRR614 pKa = 11.84 FKK616 pKa = 11.38 VRR618 pKa = 11.84 VDD620 pKa = 3.17 MSAATSPAYY629 pKa = 10.1 AGQAVVTTAISGPNDD644 pKa = 4.68 GITTTFDD651 pKa = 3.23 NQDD654 pKa = 3.36 FSDD657 pKa = 3.99 SGSNPDD663 pKa = 3.83 PNGNGDD669 pKa = 3.49 PTEE672 pKa = 4.37 AGEE675 pKa = 3.98 NDD677 pKa = 3.0 ITEE680 pKa = 4.36 VCLSNTTISYY690 pKa = 10.03 SASTYY695 pKa = 10.52 YY696 pKa = 9.85 STGSDD701 pKa = 3.48 PTPIINGVSGGTFSAPKK718 pKa = 10.13 GLVINSTSGEE728 pKa = 4.0 IDD730 pKa = 3.24 VSASIVDD737 pKa = 4.17 DD738 pKa = 4.51 YY739 pKa = 11.63 IVTYY743 pKa = 10.64 SFGGLCPSTTSISIALNPPAEE764 pKa = 4.14 PTVINQITNNTTPTLTGTAVVNVGEE789 pKa = 4.22 SLEE792 pKa = 4.05 VLLNGQTYY800 pKa = 10.7 VLGDD804 pKa = 3.78 GNLSISGTTWTLIVPVANAIGTDD827 pKa = 4.38 GIYY830 pKa = 10.8 DD831 pKa = 3.48 VTATHH836 pKa = 6.66 INGVGTRR843 pKa = 11.84 TDD845 pKa = 3.26 DD846 pKa = 3.53 TTNGEE851 pKa = 4.25 LEE853 pKa = 4.33 IDD855 pKa = 3.37 TDD857 pKa = 3.63 APEE860 pKa = 4.3 IVISGQPSIINNNSSYY876 pKa = 10.41 TVNLNFDD883 pKa = 3.58 EE884 pKa = 6.33 DD885 pKa = 3.71 ITGLQITDD893 pKa = 3.15 IVVTNASLSNLTSINASTYY912 pKa = 9.6 TIVVLPAGTGNITIDD927 pKa = 4.08 FPAGGVKK934 pKa = 10.4 DD935 pKa = 3.57 LANNNNLAASQVLTIYY951 pKa = 10.99 DD952 pKa = 3.79 DD953 pKa = 3.83 TAPATPTVNALTTSDD968 pKa = 3.62 NTPVVSGTAEE978 pKa = 3.72 IGSTVTVIINGVTFTTTADD997 pKa = 3.33 ASGNWSVDD1005 pKa = 3.14 TEE1007 pKa = 4.6 TATPTAGGPFTPLTDD1022 pKa = 3.16 GDD1024 pKa = 3.95 YY1025 pKa = 11.32 DD1026 pKa = 3.96 VAVTSTDD1033 pKa = 3.11 AAGNSSSDD1041 pKa = 3.17 ATSNEE1046 pKa = 3.55 VSIDD1050 pKa = 3.46 TTAPTAPIVNLLTTNDD1066 pKa = 3.71 VTPVITGTNGLGTSQLAGEE1085 pKa = 4.46 TLTVTVNGATYY1096 pKa = 9.57 TVTPDD1101 pKa = 3.57 ASGNWSVDD1109 pKa = 3.14 TEE1111 pKa = 4.57 TATPTSGTLGTFNDD1125 pKa = 4.13 GTSYY1129 pKa = 11.01 EE1130 pKa = 4.39 VVATVTDD1137 pKa = 3.68 AASNSTSDD1145 pKa = 3.14 ATSNEE1150 pKa = 3.67 VSIDD1154 pKa = 3.36 TTAPATPTVNTLTTSDD1170 pKa = 3.85 NTPVLSGTAEE1180 pKa = 3.77 IGSTVVVVINGVTFTTTADD1199 pKa = 3.43 ASGDD1203 pKa = 3.28 WSVDD1207 pKa = 3.17 TEE1209 pKa = 4.57 TATPTAGG1216 pKa = 3.2
Molecular weight: 124.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.592
IPC_protein 3.643
Toseland 3.414
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.592
Rodwell 3.465
Grimsley 3.312
Solomon 3.643
Lehninger 3.605
Nozaki 3.745
DTASelect 4.024
Thurlkill 3.465
EMBOSS 3.592
Sillero 3.77
Patrickios 0.795
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A2T4DSC5|A0A2T4DSC5_9BACT SanA protein OS=Marivirga lumbricoides OX=1046115 GN=C9994_06145 PE=4 SV=1
MM1 pKa = 7.59 ISALTNCRR9 pKa = 11.84 KK10 pKa = 9.69 ALKK13 pKa = 10.2 YY14 pKa = 10.1 YY15 pKa = 9.87 SAWTTTLLGCRR26 pKa = 11.84 WRR28 pKa = 11.84 TVIKK32 pKa = 8.86 TIPAAKK38 pKa = 8.9 TVTGTMPVAGTTTAPGITRR57 pKa = 11.84 CTTSIMMPLWVGLMWWILMPMSLPII82 pKa = 4.46
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.736
IPC_protein 10.131
Toseland 10.496
ProMoST 10.131
Dawson 10.628
Bjellqvist 10.321
Wikipedia 10.789
Rodwell 11.082
Grimsley 10.687
Solomon 10.687
Lehninger 10.672
Nozaki 10.54
DTASelect 10.292
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.906
IPC_peptide 10.701
IPC2_peptide 9.56
IPC2.peptide.svr19 8.397
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3452
0
3452
1013358
31
3123
293.6
33.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.448 ± 0.047
0.711 ± 0.013
5.442 ± 0.034
7.152 ± 0.044
5.208 ± 0.033
6.351 ± 0.044
1.774 ± 0.021
7.757 ± 0.036
7.348 ± 0.057
9.599 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.191 ± 0.02
5.89 ± 0.041
3.436 ± 0.026
3.716 ± 0.026
3.609 ± 0.028
6.895 ± 0.041
5.254 ± 0.044
5.986 ± 0.033
1.128 ± 0.015
4.104 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here