Klebsiella phage ST13-OXA48phi12.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J6M5|A0A482J6M5_9CAUD DNA-binding transcriptional regulator Cro OS=Klebsiella phage ST13-OXA48phi12.2 OX=2510465 PE=4 SV=1
MM1 pKa = 6.98ITGTANYY8 pKa = 10.44DD9 pKa = 3.54DD10 pKa = 5.0VPDD13 pKa = 4.16VLCALCGGYY22 pKa = 10.23FKK24 pKa = 11.16ADD26 pKa = 3.36DD27 pKa = 4.59PEE29 pKa = 4.25SHH31 pKa = 6.6EE32 pKa = 4.58CDD34 pKa = 3.05EE35 pKa = 4.33EE36 pKa = 4.27ALNEE40 pKa = 4.15IACDD44 pKa = 3.02ICGFKK49 pKa = 9.98STDD52 pKa = 3.27PDD54 pKa = 3.84GAHH57 pKa = 6.11YY58 pKa = 10.66CCEE61 pKa = 4.64DD62 pKa = 3.51NSDD65 pKa = 3.84DD66 pKa = 4.09

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J736|A0A482J736_9CAUD Uncharacterized protein OS=Klebsiella phage ST13-OXA48phi12.2 OX=2510465 PE=4 SV=1
MM1 pKa = 7.64SKK3 pKa = 10.61RR4 pKa = 11.84LNFQATFSTPYY15 pKa = 9.76SDD17 pKa = 4.08LSVRR21 pKa = 11.84SFSSGKK27 pKa = 10.02AVPALRR33 pKa = 11.84AKK35 pKa = 8.25TQHH38 pKa = 6.59RR39 pKa = 11.84PLSPNPDD46 pKa = 3.59SEE48 pKa = 4.05NQTLGGSDD56 pKa = 4.21VYY58 pKa = 11.01PLTAGVYY65 pKa = 9.72LVQNSRR71 pKa = 11.84NRR73 pKa = 11.84RR74 pKa = 11.84FAVRR78 pKa = 11.84RR79 pKa = 11.84AAFCCRR85 pKa = 11.84NDD87 pKa = 3.35TGSASNASWYY97 pKa = 10.16ACAYY101 pKa = 9.97AIAIFSRR108 pKa = 11.84IPAGSVANCSLIHH121 pKa = 6.06GASSRR126 pKa = 11.84AKK128 pKa = 9.13TVGIVTWKK136 pKa = 10.34YY137 pKa = 10.82SSS139 pKa = 3.21

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10483

37

1192

205.5

22.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.102 ± 0.631

1.278 ± 0.19

5.914 ± 0.366

6.277 ± 0.347

3.367 ± 0.185

7.135 ± 0.382

1.564 ± 0.2

5.743 ± 0.238

5.733 ± 0.507

8.404 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.133

3.997 ± 0.225

4.216 ± 0.402

4.35 ± 0.466

5.6 ± 0.231

6.973 ± 0.382

6.01 ± 0.374

6.401 ± 0.321

1.545 ± 0.156

2.881 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski