Streptococcus satellite phage Javan364
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKN0|A0A4D5ZKN0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan364 OX=2558662 GN=JavanS364_0001 PE=4 SV=1
MM1 pKa = 7.66 SIYY4 pKa = 10.79 KK5 pKa = 10.5 NGVYY9 pKa = 10.39 LGALSGKK16 pKa = 8.86 YY17 pKa = 7.7 FHH19 pKa = 7.1 HH20 pKa = 7.03 SEE22 pKa = 5.29 DD23 pKa = 3.27 GALWVDD29 pKa = 4.11 YY30 pKa = 10.04 NACQYY35 pKa = 10.62 LISVCGFPEE44 pKa = 4.49 SMFSEE49 pKa = 4.56 PEE51 pKa = 3.6 EE52 pKa = 4.09 WW53 pKa = 3.67
Molecular weight: 6.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.52
IPC2_protein 4.635
IPC_protein 4.38
Toseland 4.266
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.202
Rodwell 4.24
Grimsley 4.177
Solomon 4.317
Lehninger 4.266
Nozaki 4.457
DTASelect 4.533
Thurlkill 4.279
EMBOSS 4.215
Sillero 4.495
Patrickios 0.095
IPC_peptide 4.317
IPC2_peptide 4.495
IPC2.peptide.svr19 4.444
Protein with the highest isoelectric point:
>tr|A0A4D5ZLM0|A0A4D5ZLM0_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan364 OX=2558662 GN=JavanS364_0007 PE=4 SV=1
MM1 pKa = 7.64 TEE3 pKa = 3.44 PAYY6 pKa = 10.56 AGMKK10 pKa = 8.96 LAKK13 pKa = 9.52 EE14 pKa = 4.5 DD15 pKa = 3.43 KK16 pKa = 10.0 TKK18 pKa = 10.53 VRR20 pKa = 11.84 RR21 pKa = 11.84 PKK23 pKa = 10.5 LKK25 pKa = 9.69 QLKK28 pKa = 8.74 EE29 pKa = 3.84 RR30 pKa = 11.84 KK31 pKa = 8.27 QLKK34 pKa = 9.73 KK35 pKa = 10.38 LRR37 pKa = 11.84 KK38 pKa = 8.98 KK39 pKa = 10.02 RR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 9.88 GSS44 pKa = 3.23
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.228
IPC2_protein 10.101
IPC_protein 10.921
Toseland 11.418
ProMoST 11.345
Dawson 11.462
Bjellqvist 11.199
Wikipedia 11.711
Rodwell 11.842
Grimsley 11.477
Solomon 11.696
Lehninger 11.652
Nozaki 11.403
DTASelect 11.199
Thurlkill 11.403
EMBOSS 11.842
Sillero 11.403
Patrickios 11.564
IPC_peptide 11.711
IPC2_peptide 10.116
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2539
41
489
169.3
19.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.908 ± 0.471
0.827 ± 0.267
5.672 ± 0.424
7.995 ± 0.764
3.742 ± 0.475
5.356 ± 0.436
1.93 ± 0.211
5.829 ± 0.445
9.807 ± 0.669
10.634 ± 0.754
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.521 ± 0.304
4.805 ± 0.567
3.663 ± 0.554
3.663 ± 0.351
5.12 ± 0.584
5.553 ± 0.392
6.341 ± 0.579
4.726 ± 0.433
1.024 ± 0.2
4.884 ± 0.427
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here