Shewanella aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9QNB3|A0A6G9QNB3_9GAMM Na(+)-translocating NADH-quinone reductase subunit D OS=Shewanella aestuarii OX=1028752 GN=nqrD PE=3 SV=1
MM1 pKa = 7.34KK2 pKa = 10.22RR3 pKa = 11.84IYY5 pKa = 10.35PFALCISLVGLTACSDD21 pKa = 3.76DD22 pKa = 5.7DD23 pKa = 4.38DD24 pKa = 5.99DD25 pKa = 6.2KK26 pKa = 11.93VVTPDD31 pKa = 3.43TPEE34 pKa = 3.59VEE36 pKa = 4.4YY37 pKa = 11.07SHH39 pKa = 7.04VRR41 pKa = 11.84VIHH44 pKa = 6.39AGSDD48 pKa = 3.19APMVNVMANGAALLSDD64 pKa = 3.43VDD66 pKa = 4.02YY67 pKa = 11.77AMSSGLLEE75 pKa = 4.35VTSATYY81 pKa = 10.45DD82 pKa = 3.25IDD84 pKa = 4.1VDD86 pKa = 3.92ALLADD91 pKa = 4.21GTTLTVLEE99 pKa = 4.87ANLATEE105 pKa = 4.66ANTEE109 pKa = 4.11YY110 pKa = 10.31TAVALGTVADD120 pKa = 4.37EE121 pKa = 4.63SLMLKK126 pKa = 10.55LIANPTAEE134 pKa = 4.0IAAGYY139 pKa = 10.84ARR141 pKa = 11.84VQVLHH146 pKa = 6.2ATPSVGLVDD155 pKa = 4.21VYY157 pKa = 10.1VTAPGDD163 pKa = 4.44DD164 pKa = 3.39ISAIAPTLSANYY176 pKa = 8.8MDD178 pKa = 5.48NSTQLEE184 pKa = 4.71VPVGDD189 pKa = 3.54YY190 pKa = 10.46QIRR193 pKa = 11.84ITGSNSKK200 pKa = 10.33EE201 pKa = 3.88VVFDD205 pKa = 3.61SGTVALGDD213 pKa = 3.45MMDD216 pKa = 4.0YY217 pKa = 10.48FISAIPNTWSGDD229 pKa = 3.76SPVALHH235 pKa = 6.01VALPEE240 pKa = 4.25GQVILNDD247 pKa = 3.53INSGADD253 pKa = 2.93IRR255 pKa = 11.84VVHH258 pKa = 6.6AVADD262 pKa = 4.18APAVDD267 pKa = 3.87VFLDD271 pKa = 3.83EE272 pKa = 4.53ATTPAIDD279 pKa = 3.29MLEE282 pKa = 4.01FGKK285 pKa = 10.48IAGYY289 pKa = 10.86VNVAEE294 pKa = 4.74GQHH297 pKa = 5.2TVTVAADD304 pKa = 3.5ADD306 pKa = 3.88NSVVVIDD313 pKa = 4.08KK314 pKa = 11.26APVEE318 pKa = 4.11LMLGMSYY325 pKa = 10.65SALAIGSLSDD335 pKa = 4.09GMIEE339 pKa = 4.16PLVLTEE345 pKa = 3.59KK346 pKa = 9.4TRR348 pKa = 11.84RR349 pKa = 11.84VATEE353 pKa = 3.53AKK355 pKa = 9.53LTVTHH360 pKa = 7.03AAYY363 pKa = 10.2SAPEE367 pKa = 3.54VDD369 pKa = 4.29IYY371 pKa = 10.87LTATADD377 pKa = 3.2ISEE380 pKa = 4.45ATPALEE386 pKa = 4.58DD387 pKa = 3.73VPFKK391 pKa = 10.98ASSGSLSVAPGTYY404 pKa = 9.37TISVTVANTKK414 pKa = 10.27DD415 pKa = 3.54VAIGPLEE422 pKa = 4.22VTLEE426 pKa = 3.92AAGVYY431 pKa = 9.96GVAAVDD437 pKa = 3.77NVGGGAPFNVILLDD451 pKa = 4.29DD452 pKa = 4.1FTVMM456 pKa = 5.18

Molecular weight: 47.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9QNB1|A0A6G9QNB1_9GAMM YacL family protein OS=Shewanella aestuarii OX=1028752 GN=HBH39_14565 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84KK30 pKa = 8.78VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3605

0

3605

1213747

33

4547

336.7

37.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.806 ± 0.044

1.085 ± 0.019

5.706 ± 0.034

5.707 ± 0.037

4.129 ± 0.027

6.541 ± 0.039

2.267 ± 0.022

6.712 ± 0.037

5.423 ± 0.043

10.265 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.021

4.715 ± 0.042

3.8 ± 0.023

5.065 ± 0.041

4.117 ± 0.037

6.687 ± 0.045

5.433 ± 0.047

6.745 ± 0.032

1.135 ± 0.015

3.06 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski