Amino acid dipepetide frequency for Shewanella aestuarii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.816AlaAla: 7.816 ± 0.116
1.026AlaCys: 1.026 ± 0.029
5.168AlaAsp: 5.168 ± 0.078
5.406AlaGlu: 5.406 ± 0.09
3.39AlaPhe: 3.39 ± 0.061
5.753AlaGly: 5.753 ± 0.086
1.632AlaHis: 1.632 ± 0.037
6.363AlaIle: 6.363 ± 0.085
5.74AlaLys: 5.74 ± 0.086
9.31AlaLeu: 9.31 ± 0.097
2.585AlaMet: 2.585 ± 0.053
4.408AlaAsn: 4.408 ± 0.064
2.807AlaPro: 2.807 ± 0.059
4.207AlaGln: 4.207 ± 0.072
3.269AlaArg: 3.269 ± 0.058
5.759AlaSer: 5.759 ± 0.077
4.633AlaThr: 4.633 ± 0.096
5.539AlaVal: 5.539 ± 0.076
0.843AlaTrp: 0.843 ± 0.028
2.404AlaTyr: 2.404 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.827CysAla: 0.827 ± 0.029
0.194CysCys: 0.194 ± 0.015
0.638CysAsp: 0.638 ± 0.026
0.669CysGlu: 0.669 ± 0.032
0.479CysPhe: 0.479 ± 0.024
0.875CysGly: 0.875 ± 0.034
0.456CysHis: 0.456 ± 0.03
0.661CysIle: 0.661 ± 0.025
0.493CysLys: 0.493 ± 0.02
1.008CysLeu: 1.008 ± 0.03
0.251CysMet: 0.251 ± 0.015
0.422CysAsn: 0.422 ± 0.019
0.429CysPro: 0.429 ± 0.022
0.609CysGln: 0.609 ± 0.024
0.459CysArg: 0.459 ± 0.02
0.74CysSer: 0.74 ± 0.042
0.47CysThr: 0.47 ± 0.026
0.68CysVal: 0.68 ± 0.026
0.127CysTrp: 0.127 ± 0.011
0.361CysTyr: 0.361 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.746AspAla: 4.746 ± 0.072
0.621AspCys: 0.621 ± 0.023
3.389AspAsp: 3.389 ± 0.074
3.841AspGlu: 3.841 ± 0.065
2.63AspPhe: 2.63 ± 0.047
3.877AspGly: 3.877 ± 0.119
1.037AspHis: 1.037 ± 0.029
4.464AspIle: 4.464 ± 0.065
3.634AspLys: 3.634 ± 0.055
5.287AspLeu: 5.287 ± 0.072
1.484AspMet: 1.484 ± 0.036
2.913AspAsn: 2.913 ± 0.051
2.006AspPro: 2.006 ± 0.045
1.892AspGln: 1.892 ± 0.047
2.058AspArg: 2.058 ± 0.041
3.404AspSer: 3.404 ± 0.062
2.976AspThr: 2.976 ± 0.062
3.932AspVal: 3.932 ± 0.072
0.81AspTrp: 0.81 ± 0.027
2.058AspTyr: 2.058 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
4.779GluAla: 4.779 ± 0.072
0.495GluCys: 0.495 ± 0.025
2.717GluAsp: 2.717 ± 0.056
2.796GluGlu: 2.796 ± 0.058
2.483GluPhe: 2.483 ± 0.055
3.066GluGly: 3.066 ± 0.06
1.497GluHis: 1.497 ± 0.043
3.652GluIle: 3.652 ± 0.06
3.16GluLys: 3.16 ± 0.06
6.793GluLeu: 6.793 ± 0.092
1.538GluMet: 1.538 ± 0.034
2.445GluAsn: 2.445 ± 0.062
1.903GluPro: 1.903 ± 0.048
4.129GluGln: 4.129 ± 0.063
2.805GluArg: 2.805 ± 0.058
3.422GluSer: 3.422 ± 0.053
2.931GluThr: 2.931 ± 0.054
4.171GluVal: 4.171 ± 0.073
0.593GluTrp: 0.593 ± 0.022
1.695GluTyr: 1.695 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.736PheAla: 3.736 ± 0.063
0.525PheCys: 0.525 ± 0.022
2.954PheAsp: 2.954 ± 0.047
2.66PheGlu: 2.66 ± 0.057
1.663PhePhe: 1.663 ± 0.043
3.046PheGly: 3.046 ± 0.056
0.76PheHis: 0.76 ± 0.024
2.972PheIle: 2.972 ± 0.055
2.173PheLys: 2.173 ± 0.049
3.112PheLeu: 3.112 ± 0.056
1.044PheMet: 1.044 ± 0.033
2.442PheAsn: 2.442 ± 0.043
1.311PhePro: 1.311 ± 0.028
1.158PheGln: 1.158 ± 0.034
1.326PheArg: 1.326 ± 0.033
3.381PheSer: 3.381 ± 0.055
2.372PheThr: 2.372 ± 0.055
2.746PheVal: 2.746 ± 0.053
0.484PheTrp: 0.484 ± 0.021
1.425PheTyr: 1.425 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.041GlyAla: 5.041 ± 0.083
0.887GlyCys: 0.887 ± 0.029
3.518GlyAsp: 3.518 ± 0.06
3.946GlyGlu: 3.946 ± 0.071
3.34GlyPhe: 3.34 ± 0.057
4.381GlyGly: 4.381 ± 0.086
1.584GlyHis: 1.584 ± 0.036
4.702GlyIle: 4.702 ± 0.077
3.892GlyLys: 3.892 ± 0.061
6.785GlyLeu: 6.785 ± 0.096
1.967GlyMet: 1.967 ± 0.048
2.668GlyAsn: 2.668 ± 0.055
1.57GlyPro: 1.57 ± 0.038
2.876GlyGln: 2.876 ± 0.051
2.805GlyArg: 2.805 ± 0.057
3.975GlySer: 3.975 ± 0.117
3.215GlyThr: 3.215 ± 0.08
4.965GlyVal: 4.965 ± 0.086
0.797GlyTrp: 0.797 ± 0.028
2.495GlyTyr: 2.495 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.686HisAla: 1.686 ± 0.041
0.352HisCys: 0.352 ± 0.019
1.168HisAsp: 1.168 ± 0.031
1.008HisGlu: 1.008 ± 0.033
1.109HisPhe: 1.109 ± 0.031
1.546HisGly: 1.546 ± 0.037
0.754HisHis: 0.754 ± 0.029
1.574HisIle: 1.574 ± 0.041
1.127HisLys: 1.127 ± 0.03
2.342HisLeu: 2.342 ± 0.052
0.479HisMet: 0.479 ± 0.021
1.024HisAsn: 1.024 ± 0.03
1.1HisPro: 1.1 ± 0.03
1.409HisGln: 1.409 ± 0.036
0.989HisArg: 0.989 ± 0.03
1.464HisSer: 1.464 ± 0.04
1.037HisThr: 1.037 ± 0.033
1.28HisVal: 1.28 ± 0.033
0.34HisTrp: 0.34 ± 0.018
0.882HisTyr: 0.882 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.827IleAla: 6.827 ± 0.091
0.772IleCys: 0.772 ± 0.026
4.812IleAsp: 4.812 ± 0.074
4.826IleGlu: 4.826 ± 0.066
2.264IlePhe: 2.264 ± 0.054
4.732IleGly: 4.732 ± 0.079
1.343IleHis: 1.343 ± 0.037
4.1IleIle: 4.1 ± 0.074
3.828IleLys: 3.828 ± 0.064
5.275IleLeu: 5.275 ± 0.084
1.406IleMet: 1.406 ± 0.037
3.859IleAsn: 3.859 ± 0.063
2.604IlePro: 2.604 ± 0.045
2.404IleGln: 2.404 ± 0.043
2.624IleArg: 2.624 ± 0.047
4.995IleSer: 4.995 ± 0.078
3.922IleThr: 3.922 ± 0.093
4.064IleVal: 4.064 ± 0.064
0.639IleTrp: 0.639 ± 0.023
1.822IleTyr: 1.822 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
5.176LysAla: 5.176 ± 0.078
0.379LysCys: 0.379 ± 0.023
2.66LysAsp: 2.66 ± 0.045
2.664LysGlu: 2.664 ± 0.056
1.705LysPhe: 1.705 ± 0.042
3.353LysGly: 3.353 ± 0.051
1.376LysHis: 1.376 ± 0.038
3.204LysIle: 3.204 ± 0.058
2.749LysLys: 2.749 ± 0.054
5.959LysLeu: 5.959 ± 0.073
1.574LysMet: 1.574 ± 0.036
2.313LysAsn: 2.313 ± 0.054
2.461LysPro: 2.461 ± 0.051
3.551LysGln: 3.551 ± 0.064
2.656LysArg: 2.656 ± 0.047
3.39LysSer: 3.39 ± 0.057
2.936LysThr: 2.936 ± 0.052
4.045LysVal: 4.045 ± 0.065
0.568LysTrp: 0.568 ± 0.027
1.51LysTyr: 1.51 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
10.271LeuAla: 10.271 ± 0.111
1.1LeuCys: 1.1 ± 0.03
5.847LeuAsp: 5.847 ± 0.066
5.296LeuGlu: 5.296 ± 0.078
4.134LeuPhe: 4.134 ± 0.062
6.476LeuGly: 6.476 ± 0.087
2.029LeuHis: 2.029 ± 0.049
6.576LeuIle: 6.576 ± 0.082
5.481LeuLys: 5.481 ± 0.082
10.444LeuLeu: 10.444 ± 0.14
2.78LeuMet: 2.78 ± 0.054
4.973LeuAsn: 4.973 ± 0.064
4.564LeuPro: 4.564 ± 0.071
3.993LeuGln: 3.993 ± 0.064
3.998LeuArg: 3.998 ± 0.07
7.787LeuSer: 7.787 ± 0.091
6.482LeuThr: 6.482 ± 0.083
6.838LeuVal: 6.838 ± 0.086
1.002LeuTrp: 1.002 ± 0.029
2.581LeuTyr: 2.581 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.664MetAla: 2.664 ± 0.05
0.204MetCys: 0.204 ± 0.014
1.252MetAsp: 1.252 ± 0.031
1.137MetGlu: 1.137 ± 0.033
0.901MetPhe: 0.901 ± 0.03
1.67MetGly: 1.67 ± 0.04
0.513MetHis: 0.513 ± 0.019
1.498MetIle: 1.498 ± 0.038
1.41MetLys: 1.41 ± 0.032
2.962MetLeu: 2.962 ± 0.065
0.808MetMet: 0.808 ± 0.029
1.142MetAsn: 1.142 ± 0.032
1.25MetPro: 1.25 ± 0.028
1.269MetGln: 1.269 ± 0.033
1.135MetArg: 1.135 ± 0.03
1.97MetSer: 1.97 ± 0.043
1.669MetThr: 1.669 ± 0.04
1.746MetVal: 1.746 ± 0.039
0.26MetTrp: 0.26 ± 0.015
0.566MetTyr: 0.566 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.866AsnAla: 3.866 ± 0.07
0.511AsnCys: 0.511 ± 0.02
2.837AsnAsp: 2.837 ± 0.076
2.642AsnGlu: 2.642 ± 0.056
1.683AsnPhe: 1.683 ± 0.039
3.199AsnGly: 3.199 ± 0.068
1.158AsnHis: 1.158 ± 0.033
3.201AsnIle: 3.201 ± 0.056
2.616AsnLys: 2.616 ± 0.049
4.391AsnLeu: 4.391 ± 0.064
1.125AsnMet: 1.125 ± 0.03
2.419AsnAsn: 2.419 ± 0.068
2.262AsnPro: 2.262 ± 0.045
2.776AsnGln: 2.776 ± 0.068
2.097AsnArg: 2.097 ± 0.042
2.936AsnSer: 2.936 ± 0.064
2.583AsnThr: 2.583 ± 0.061
2.824AsnVal: 2.824 ± 0.052
0.599AsnTrp: 0.599 ± 0.023
1.424AsnTyr: 1.424 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
3.074ProAla: 3.074 ± 0.055
0.309ProCys: 0.309 ± 0.015
2.254ProAsp: 2.254 ± 0.041
2.774ProGlu: 2.774 ± 0.056
1.687ProPhe: 1.687 ± 0.04
2.092ProGly: 2.092 ± 0.049
0.835ProHis: 0.835 ± 0.028
2.708ProIle: 2.708 ± 0.054
2.14ProLys: 2.14 ± 0.04
4.026ProLeu: 4.026 ± 0.063
0.999ProMet: 0.999 ± 0.033
1.859ProAsn: 1.859 ± 0.047
1.05ProPro: 1.05 ± 0.03
1.928ProGln: 1.928 ± 0.047
1.205ProArg: 1.205 ± 0.032
2.447ProSer: 2.447 ± 0.05
2.131ProThr: 2.131 ± 0.048
2.847ProVal: 2.847 ± 0.05
0.47ProTrp: 0.47 ± 0.02
1.163ProTyr: 1.163 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
4.948GlnAla: 4.948 ± 0.083
0.47GlnCys: 0.47 ± 0.03
2.3GlnAsp: 2.3 ± 0.053
2.076GlnGlu: 2.076 ± 0.039
2.097GlnPhe: 2.097 ± 0.047
3.175GlnGly: 3.175 ± 0.055
1.423GlnHis: 1.423 ± 0.043
3.089GlnIle: 3.089 ± 0.054
2.176GlnLys: 2.176 ± 0.051
5.895GlnLeu: 5.895 ± 0.103
1.267GlnMet: 1.267 ± 0.03
1.78GlnAsn: 1.78 ± 0.043
1.778GlnPro: 1.778 ± 0.045
4.258GlnGln: 4.258 ± 0.111
2.239GlnArg: 2.239 ± 0.041
3.113GlnSer: 3.113 ± 0.057
2.736GlnThr: 2.736 ± 0.05
3.524GlnVal: 3.524 ± 0.048
0.685GlnTrp: 0.685 ± 0.025
1.621GlnTyr: 1.621 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
3.025ArgAla: 3.025 ± 0.056
0.444ArgCys: 0.444 ± 0.031
2.302ArgAsp: 2.302 ± 0.041
2.371ArgGlu: 2.371 ± 0.05
2.031ArgPhe: 2.031 ± 0.039
2.403ArgGly: 2.403 ± 0.049
1.078ArgHis: 1.078 ± 0.029
2.939ArgIle: 2.939 ± 0.05
2.22ArgLys: 2.22 ± 0.05
4.534ArgLeu: 4.534 ± 0.066
1.108ArgMet: 1.108 ± 0.033
1.812ArgAsn: 1.812 ± 0.04
1.453ArgPro: 1.453 ± 0.033
2.226ArgGln: 2.226 ± 0.048
1.98ArgArg: 1.98 ± 0.046
2.372ArgSer: 2.372 ± 0.049
1.835ArgThr: 1.835 ± 0.04
2.815ArgVal: 2.815 ± 0.046
0.557ArgTrp: 0.557 ± 0.022
1.668ArgTyr: 1.668 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.635SerAla: 5.635 ± 0.081
0.715SerCys: 0.715 ± 0.035
3.722SerAsp: 3.722 ± 0.06
3.683SerGlu: 3.683 ± 0.064
2.957SerPhe: 2.957 ± 0.071
4.687SerGly: 4.687 ± 0.084
1.621SerHis: 1.621 ± 0.038
4.536SerIle: 4.536 ± 0.063
3.357SerLys: 3.357 ± 0.061
7.182SerLeu: 7.182 ± 0.077
1.71SerMet: 1.71 ± 0.038
2.96SerAsn: 2.96 ± 0.073
2.577SerPro: 2.577 ± 0.054
3.581SerGln: 3.581 ± 0.059
2.756SerArg: 2.756 ± 0.05
4.512SerSer: 4.512 ± 0.094
3.313SerThr: 3.313 ± 0.077
4.496SerVal: 4.496 ± 0.073
0.743SerTrp: 0.743 ± 0.025
2.126SerTyr: 2.126 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.607ThrAla: 4.607 ± 0.093
0.548ThrCys: 0.548 ± 0.032
3.166ThrAsp: 3.166 ± 0.064
3.233ThrGlu: 3.233 ± 0.057
2.07ThrPhe: 2.07 ± 0.044
3.906ThrGly: 3.906 ± 0.077
1.254ThrHis: 1.254 ± 0.032
3.522ThrIle: 3.522 ± 0.068
2.555ThrLys: 2.555 ± 0.052
6.163ThrLeu: 6.163 ± 0.091
1.104ThrMet: 1.104 ± 0.036
2.433ThrAsn: 2.433 ± 0.069
2.669ThrPro: 2.669 ± 0.052
2.798ThrGln: 2.798 ± 0.059
2.065ThrArg: 2.065 ± 0.042
3.548ThrSer: 3.548 ± 0.081
2.983ThrThr: 2.983 ± 0.07
3.598ThrVal: 3.598 ± 0.073
0.573ThrTrp: 0.573 ± 0.025
1.532ThrTyr: 1.532 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
6.257ValAla: 6.257 ± 0.087
0.748ValCys: 0.748 ± 0.034
4.161ValAsp: 4.161 ± 0.071
4.163ValGlu: 4.163 ± 0.066
2.731ValPhe: 2.731 ± 0.055
4.37ValGly: 4.37 ± 0.076
1.15ValHis: 1.15 ± 0.032
4.955ValIle: 4.955 ± 0.065
3.683ValLys: 3.683 ± 0.061
6.254ValLeu: 6.254 ± 0.087
1.865ValMet: 1.865 ± 0.044
3.573ValAsn: 3.573 ± 0.071
2.412ValPro: 2.412 ± 0.04
2.212ValGln: 2.212 ± 0.043
2.459ValArg: 2.459 ± 0.053
4.891ValSer: 4.891 ± 0.068
4.112ValThr: 4.112 ± 0.086
4.924ValVal: 4.924 ± 0.082
0.668ValTrp: 0.668 ± 0.023
1.861ValTyr: 1.861 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.77TrpAla: 0.77 ± 0.025
0.152TrpCys: 0.152 ± 0.011
0.573TrpAsp: 0.573 ± 0.02
0.415TrpGlu: 0.415 ± 0.018
0.528TrpPhe: 0.528 ± 0.021
0.689TrpGly: 0.689 ± 0.027
0.363TrpHis: 0.363 ± 0.018
0.661TrpIle: 0.661 ± 0.027
0.425TrpLys: 0.425 ± 0.019
1.546TrpLeu: 1.546 ± 0.039
0.316TrpMet: 0.316 ± 0.016
0.429TrpAsn: 0.429 ± 0.019
0.461TrpPro: 0.461 ± 0.021
1.078TrpGln: 1.078 ± 0.034
0.625TrpArg: 0.625 ± 0.023
0.657TrpSer: 0.657 ± 0.025
0.451TrpThr: 0.451 ± 0.022
0.7TrpVal: 0.7 ± 0.023
0.169TrpTrp: 0.169 ± 0.012
0.349TrpTyr: 0.349 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.308TyrAla: 2.308 ± 0.041
0.395TyrCys: 0.395 ± 0.021
1.619TyrAsp: 1.619 ± 0.041
1.463TyrGlu: 1.463 ± 0.044
1.403TyrPhe: 1.403 ± 0.039
2.112TyrGly: 2.112 ± 0.048
0.806TyrHis: 0.806 ± 0.025
1.742TyrIle: 1.742 ± 0.04
1.373TyrLys: 1.373 ± 0.038
3.389TyrLeu: 3.389 ± 0.054
0.614TyrMet: 0.614 ± 0.025
1.276TyrAsn: 1.276 ± 0.04
1.33TyrPro: 1.33 ± 0.04
2.296TyrGln: 2.296 ± 0.053
1.624TyrArg: 1.624 ± 0.035
2.109TyrSer: 2.109 ± 0.053
1.538TyrThr: 1.538 ± 0.058
1.716TyrVal: 1.716 ± 0.043
0.429TyrTrp: 0.429 ± 0.018
1.059TyrTyr: 1.059 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3605 proteins (1213747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski